Start Date: Feb 17, 2006
End Date: Dec 15, 2010
Time of entry of Salmonella and Campylobacter will be monitored in turkeys. The study will document flock management practices which affect the prevalence of these foodborne pathogens initially in the brooder period and ultimately throughout live production. Key virulence attributes of C. jejuni and C. coli strains recovered from turkeys will be characterized in vitro (cell invasion assays)and in vivo (day of hatch poult model.) Ultimately, differential gene expression formats will be used to differentiate a virulent from virulent isolates of C. coli and C. jejuni. Bacterial and fungal communities of the ceca of domestic and wild turkeys will be described. This initial survey will provide a measurement of diversity between wild and domestic birds. Analysis of total community flux over time will focus on kinetics of Campylobacter community development and stability in the turkey ceca. Organisms that correlate with Campylobacter colonization or exclusion will be identified. Second generation PCR assays will be developed to detect and quantify Salmonella and Campylobacter on hog and turkey carcasses. Monitoring viscera will serve as an indicator of on-farm versus in-plant sources of contamination. These studies will assist in determining the critical control points of contamination during slaughter. IBC 0312 Certified 10/23/09. IBC 0331 Certified 02/16/10.