Start Date: Apr 09, 2006
End Date: Sep 30, 2010
Investigate isoform specificity for nitrate reductase (NR) posttranslational modification in vivo, and elucidate the impact of 14-3-3 binding on NR protein degradation. Localize the membrane binding site(s) on sucrose synthase and identify factors that may control the interaction. Investigate the biochemical basis of the light regulation of Rubisco/activase in species that lack the redox-regulated large isoform of activase. Further elucidate the mechanism of the interaction between Rubisco and activase. Use high-resolution spatial and temporal analysis of leaf growth to identify specific areas where leaf growth is occurring. Determine the biochemical factors responsible for the lower activation state of Rubisco, at high temperatures and test potential transgenic amelioration strategies. Determine the biochemical basis for the "Green Seed Problem" of canola. Perform metabolite analysis of growing leaves under elevated CO2 and O3 to identify key components that may be involved in controlling growth. Determine the factors that lower the activation state of Rubisco under sink- and/or N-limited conditions, which are often encountered when plants are grown under high CO2. Explore the interaction of elevated O3 with drought on soybean performance. Determine if growth at elevated CO2 enhances or ameliorates oxidative stress.