2007 Annual Report
1931-21000-016-03R - Reimbursable Cooperative Agreement with The Pennsylvania State University, Cornell University, and the USDA-CSREES National Research Initiative (NRI). ESTs associated with fire blight infection were identified. Project is monitored through monthly conference calls among the project members. For a complete report on the progress of this agreement, see the report for 1931-21000-016-03R.
1931-21000-016-04G - Assistance-type Cooperative Agreement with the Pennsylvania State University. Genes associated with fire blight infection were identified. Progress is monitored through monthly conference calls among the project members. For a complete report on the progress of this agreement, see the report for 1931-21000-016-04G.
1931-21000-016-05G - Assistance-type Cooperative Agreement with Cornell University. Gene expression of fire blight resistant and susceptible cultivars was measured. Progress is monitored through monthly conference calls among the project members. For a complete report on the progress of this agreement, see the report for 1931-21000-016-05G.
1931-21000-016-06S - Specific Cooperative Agreeement with the University of Illinois. The objective of this agreement is to produce a 40,000 gene apple microarray to be used for functional genomic studies in the Rosaceae. Progress: The first printing of the microarray was completed in July, 2007. The quality of the array is currently being assessed by both parties. Progess is monitored through bi-weekly to monthly phone conferences to deal with critical questions regarding the design, printing, and quality analysis of the microarray. For a complete report on the progress of this agreement, see the report for 1931-21000-016-06S.
For a complete report on the progress of these subordinate projects, see the corresponding annual report.
TITLE: Identification of Genes Associated with Apple’s Response to Fire Blight Disease. PROBLEM: Fire blight is a destructive disease of apple and pear trees that is estimated to cost the U.S. fruit industry over $100 million a year in crop losses and disease control. ACCOMPLISHMENT: A technique known as 'suppression subtractive cDNA hybridization' was used to identify genes in apple that respond to fire blight infection. A total of 468 apple genes were identified that responded to the disease between 1 and 72 hours after challenge with the fire blight pathogen. The DNA sequence of these genes was deposited in a publicly accessible database maintained by the National Center for Biotechnology Information (http://www.ncbi.nlm.nih.gov). IMPACT: This genetic information will be used by biologists, horticulturalists, and plant breeders to develop new strategies for improving plant resistance to fire blight disease. NATIONAL PROGRAM COMPONENT 2: Plant Molecular and Biological Processes. NATIONAL PROGRAM PROBLEM STATEMENT 2B: Plant Interactions with their Environment.
Bassett, C.L. 2007. Control of gene expression by mRNA transport and turnover. In: Bassett, C.L., editors. Regulation of Gene Expression in Plants: The Role of Transcript Structure and Processing. New York, NY: Springer Life Sciences. p. 148-188.
Farrell, Jr., R., Bassett, C.L. 2007. Multiple transcript initiation as a mechanism for regulating gene expression. In: Bassett, C.L., editor. Regulation of Gene Expression in Plants: The Role of Transcript Structure and Processing. New York, NY: Springer Life Sciences. p. 39-66.
Bassett, C.L., Wisniewski, M.E., Farrell Jr., R., Artlip, T.S., Norelli, J.L. 2006. Global analysis of genes regulated by low temperature and photoperiod in peach bark. Journal of the American Society for Horticultural Science 131:551-563.
Wang, Y., Meilan, R., Wisniewski, M.E., Cui, M., Fuchigami, L. 2007. Transgenic tomato (Lycopersicon esculentum) that overexpress cAPX exhibits enhanced tolerance to UV-B and heat stress. Journal of Applied Horticulture. 8:1-4.
Kalberer, S., Wisniewski, M.E., Arora, R. 2006. Deacclimation and reacclimation of cold-hardy plants: current understanding and emerging concepts. Plant Science. 171:3-16.