Start Date: Mar 01, 2006
End Date: Sep 30, 2010
Single quantitative trait locus (QTL) lines for loci involved in FHB and mycotoxin resistance from Zhedar 2 will be developed by backcrossing, fine-mapped using all available molecular markers, and characterized in replicated field trials. Candidate genes from collaborative projects will be verified by inserting the genes into susceptible cultivars via transformation, and testing homozygous progeny in the field. RWA resistance genes in ARS germplasm releases will be mapped using a variety of molecular markers. The involvement of ethylene in tissue culture regeneration will be tested will be tested by manipulating ethylene levels with inhibitors and precursors. Optimal timing for ethylene exposure will be determined and tested using commercial barley cultivars. Genes responsible for ethylene perception, synthesis and regulation will be isolated and characterized by following their expression through the tissue culture process. Antifungal and antitoxin genes will be inserted into a commercial barley cultivar using particle bombardment. Resulting lines will be tested in the lab for gene expression using northern and western analyses, and in the field for reaction FHB and other diseases. BSL1 & BSL1-P, 6-23-05.