IDENTIFICATION AND CHARACTERIZATION OF PEST INSECT IMMUNE RESPONSES TO BIOLOGICAL CONTROL AGENTS
Project Number: 3622-22000-031-00
Start Date: Oct 01, 2005
End Date: Mar 25, 2010
To optimize the efficacy of biological control agents for integrated management of pests through the following: 1) Develop substantial new knowledge on host innate immune reactions to viral infection and determine important differences in the immune mechanisms to viral and bacterial infection; 2) Develop important new knowledge on the biological roles of selenium, other micronutrients and phytonutrients in insect immune reactions to challenge; 3) Develop new information on prostaglandins and other biochemical signals responsible for mediating and coordinating insect immune reactions to challenge; and 4)Develop new methods to inhibit synthesis of key mediators (eicosanoids) of insect immunity.
1) Identify immune response factors responsible for resistance to entomopathogenic viruses, bacteria and parasitoids using proteomic, mass spectrometric, liquid chromatographic, and genomic methods; 2) Isolate and clone selected immune response factors for detailed characterization; 3) Determine the mechanisms by which these immune response factors determine virulence and host range; 4) Test the effects of foliar immunosuppressive and immunostimulatory factors upon the activities or levels of immune response factors; and 5) Determine and characterize the role of eicosanoids in the biochemical regulatory pathways coordinating immune response factors using in vitro and in vivo approaches.