2008 Annual Report
Pathogen Detection on Agar Plates. Campylobacter commonly found in poultry is the leading foodborne pathogen causing diarrhea illness. Current methods for detecting and identifying Campylobacter are time-consuming and complicated. We are developing an imaging method, known as hyperspectral imaging, to discriminate Campylobacter cultures growing in agar from non-pathogenic cultures commonly found in poultry processing. Once fully developed, the imaging system is expected to automatically locate and identify Campylobacters grown on agar plates from non-pathogenic organisms and will have the potential to reduce the incubation time and identify other pathogenic bacteria (Objective 2b).
Fertility of Broiler Hatching Eggs. Approximately 30 billion broiler hatching eggs are produced in the world each year. Up to 10 percent are not fertile yet are incubated anyway. An accurate and automated system to remove these eggs prior to incubation would be an invaluable aid to poultry hatcheries (Objective 2b). A camera system was configured to image fertile and infertile eggs prior to incubation and then each during the first 3 days of incubation. Data from the camera was then analyzed with a mathematical modeling process to separate fertile from infertile eggs. Although the initial testing and analysis was promising, eventually the model system did not produce results that were accurate enough to be considered for a commercial system. Further analysis and modeling of the collected data will continue.
Nanotechnology for Food Pathogen Detection We used nanotechnology to develop a method for surface plasmon resonance for use in chemical and biological sensors. It is known that metallic nanoparticles can exhibit localized surface plasmon resonance (LSPR) when excited by light due to the collective oscillation of metallic nanoparticles. However, for sensing applications, it is very important to engineer the nanostructure so that one can adjust the LSPR wavelength that is excited. We developed a simple but versatile fabrication technique to tune the LSPR excitation wavelength of silver thin films to shorter wavelengths in the blue region. Since different applications require specific LSPR wavelengths and absorbance spectra, an optimal surface passivation method is being studied for foodborne pathogen detection, which can be of great potential in identifying different food safety and security contaminants (Objective 2b and 3).
5.Significant Activities that Support Special Target Populations
Berrang, M.E., Meinersmann, R.J., Smith, D.P., Zhuang, H. 2008. The effect of chilling in cold air or ice water on the microbiological quality of broiler carcasses and the population of Campylobacter. Poultry Science. 87(5):992-998.
Hannah, J.F., Fletcher, D.L., Cox Jr, N.A., Smith, D.P., Cason Jr, J.A., Northcutt, J.K., Buhr, R.J., Richardson, L.J. 2008. Effect of sand and shaking duration on the recovery of aerobic bacteria, coliforms, and Escherichia coli from prechill broiler whole carcass rinsates. Applied Poultry Research. 17:(2)272-277.
Huezo, R., Northcutt, J.K., Smith, D.P., Fletcher, D.L. 2007. Effect of chilling method and post-mortem aging time on broiler breast fillet quality. Journal of Applied Poultry Research. 16:537-545.
Kise, M., Park, B., Lawrence, K.C., Windham, W.R. 2007. Design and Calibration of a Dual-band Imaging System. Sensing and Instrumentation for Food Quality and Safety. 1(3): 113-121. Kise, M., Park, B., Lawrence, K.C., Windham, W.R. 2008. Development of Handheld Multispectral Imaging For Food Safety Inspection. Biological Engineering (ASABE).
Lawrence, K.C., Yoon, S.C., Heitschmidt, G.W., Jones, D.R., Park, B. 2008. Imaging system with modified-pressure chamber for crack detection in shell-eggs. Sensing and Instrumentation for Food Quality and Safety.2:116-122
Northcutt, J.K., Huezo, R.I., Ingram, K.D., Smith, D.P. 2008. Microbiology of Broiler Carcasses and Chemistry of Chiller Water as Affected by Water Reuse. Poultry Science. 87:1458-1463.
Park, B., Kise, M., Windham, W.R., Lawrence, K.C., Yoon, S.C. 2008. Textural Analysis of Hyperspectral Images for Improving Contaminant Detection Accuracy. Sensing and Instrumentation for Food Quality and Safety. 2(3):208-214.
Smith, D.P., Musgrove, M.T. 2008. Effect of blood spots on table egg albumen on Salmonella growth. Poultry Science. 87: 1659-1661
Windham, W.R., Heitschmidt, G.W., Lawrence, K.C., Park, B., Smith, D.P. 2008. Effect of spectrally mixed pixels on detection of cecal contaminated broiler carcasses. International Journal of Poultry Science. 6(12):955-959.
Fu, J., Park, B., Siragusa, G.R., Jones, L., Tripp, R.A., Zhao, Y., Cho, Y. 2008. Au/Si Hetero-Nanorod-based Biosensor for Salmonella Detection. Nanotechnoloyg 19: 155502.
Yoon, S.C., Lawrence, K.C., Smith, D.P., Park, B., Windham, W.R. 2008. Bone fragment detection in chicken breast fillets using transmittance image enhancement. Transactions of the ASABE 51 (1): 331-339.
Yoon, S.C., Lawrence, K.C., Smith, D.P., Park, B., Windham, W.R. 2008. Embedded bone fragment detection in poultry using transmittance image enhancement and hyperspectral reflectance imaging. Sensing and Instrumentation for Food Quality and Safety, 10.1007/s11694-008-9044-1 (online version).
Park, B. 2008. Quality Inspection of Poultry Carcasses. Food Quality and Preference. Chapter 7 in Computer Vision Technology for Food Quality Evaluation (ed. Da-Wen Sun). Elsevier Press: 157-187. 2008