Start Date: Dec 28, 2005
End Date: Dec 27, 2010
Resistance to aflatoxin contamination will be enhanced in corn and cottonseed through marker assisted breeding and genetic engineering, respectively. In order to accomplish these goals, complex natural resistance mechanisms in corn kernels will be elucidated in resistant corn inbreds through identification of resistance associated proteins using proteomics and other resistance associated compounds through chemical analysis. Understanding the molecular basis of seed based resistances will lead to identification of biochemical factors correlated with resistance for use in marker assisted breeding and/or when pertinent resistance genes are identified and cloned, for use in enhancement of resistance in crops through genetic engineering. This strategy is especially pertinent to cottonseed, which does not possess practical levels of natural resistance to aflatoxin producing fungi in its germplasm base. Another goal is to assess resistance related biochemical products for their stability of expression in native and transgenic crops under environmental conditions (e.g. drought) known to be conducive to aflatoxin contamination. Also as a part of this project, rapid, non destructive detection methodology based upon hyperspectral imaging will be developed to measure fungal growth and aflatoxin in corn kernels and spectral signatures associated with traits for resistance to fungal infection and aflatoxin contamination in corn kernels, and also to measure physical and biochemical attributes in kernels potentially useful in resistance marker selection.