Start Date: Dec 10, 2005
End Date: Dec 09, 2010
(1) Use a novel reporter system and transposon mutagenesis o identifiy DT104 genes involved in rumen protozoa-mediated hypervirulence. (2) Use an in vitro conjugation system to chracterize intra-protozoal transfer of plasmids from Klebsiella (and other bacteria) to Salmonella. (3) Compare the progression of salmonellosis for defaunated and fully-faunated calves infected with DT104. (4) Functional genomic analyses will be performed on Salmonella exposed to norepinephrine, low pH, and conditions relevant to intestinal colonization. (5) Use microarrays to identify swine genes that contribute to innate resistance to Salmonella colonization. IBC-0210R BSL-Exempt; Recertified 11/02/09.