2010 Annual Report
Research is needed to enhance biological control of insects and mites through improved methods for rearing and deploying arthropod predators. The impact of predators can be increased through discovery of essential nutritional factors from prey or hosts that increase reproductive efficiency. Following bioassay-guided isolation and characterization, these factors will be employed as additives for artificial diets. Biochemical assays of contents of these factors in prey will also allow estimates of prey quality as food for predators. Biochemical and behavioral analyses of predators will produce estimates of fitness in insectary and field. Correlations of predator fitness with species and abundance of prey in field-cages will yield knowledge of the quality of prey as food and the quality of predators introduced into cages after rearing on selected diets in the laboratory. Predatory efficiency of generalists such as Orius insidiosus, Podisus maculiventris, and Geocoris punctipes will be assessed through predation rates and selection of prey. Their reproductive potential will be assessed as egg load. General fitness of the predators will be measured through quantitative and qualitative analyses of lipids and proteins. Results will yield commercial additives for artificial diets for predators, biochemical markers for assessment of insect quality, and tools for improved management of existing and introduced populations of predators and pest populations in the field and greenhouse.
This project has termed and been replaced by project #6615-22000-024-00D.
Mena-Correa, J., Sivinski, J.M., Anzures-Dadda, A., Ramirez-Romero, R., Gates, M.W., Aluja, M. 2010. Consideration of Eurytoma sivinskii Gates and Grissell, a eurytomid (Hymenoptera) with unusual foraging behaviors, as a biological control agent of tephritid (Diptera) fruit flies. Biological Control. 53:9-17.
Joyce, A.L., Aluja, M., Sivinski, J.M., Vinson, B., Ramirez-Romero, R., Bernal, J.S., Guillen, L. 2010. Effect of continuous rearing on courtship acoustics of five braconid parasitoids, candidates for augmentative biological control of Anastrepha species. Biocontrol. 55:573-582.
Aluja, M., Rull, J., Sivinski, J.M., Trujillo, G., Perez-Staples, D. 2009. Male and female condition influence mating performance and sexual receptivity in two tropical fruit flies (Diptera: Tephritidae) with contrasting life histories. Journal of Insect Physiology. 55:1091-1098.
Lampson, B., Han, Y., Khalilian, A., Greene, J., Mankin, R.W., Foreman, E.G. 2010. Characterization of substrate-borne vibrational signals of Euschistus servus (Heteroptera: Pentatomidae). American Journal of Agricultural and Biological Science. 5:32-36.
Prasifka, J.R., Bradshaw, J.D., Meagher Jr, R.L., Nagoshi, R.N., Gray, M.E., Steffey, K.L. 2009. Development and feeding of fall armyworm, Spodoptera frugiperda, on Miscanthus x giganteus and switchgrass. Journal of Economic Entomology. 102(6):2154-2159.
Holler, T.C., Peebles, M., Young, A., Whiteman, L., Olson, S., Sivinski, J.M. 2009. Efficacy of the Suterra biolure individual female fruit fly attractant packages vs. the Unipak version. Florida Entomologist. 92(4):667-669.
Nirmala, X., Zimowska, G., Handler, A.M. 2009. Characterization of the proteasome ß2 subunit gene and its mutant allele in the tephritid fruit fly pest, Anastrepha suspensa. Insect Molecular Biology. 18(3):333-340.
Nagoshi, R.N. 2010. The fall armyworm Triose phosphate isomerase (Tpi) gene as a marker of strain identity and interstrain mating. Annals of the Entomological Society of America. 103(2):283-292.
Nagoshi, R.N., Meagher Jr, R.L., Jenkins, D.A. 2010. Puerto Rico fall armyworm has only limited interactions with those from Brazil or Texas, but could have substantial exchanges with Florida populations. Journal of Economic Entomology. 103(2):360-367.
Zimowaska, G.J., Nirmala, X., Handler, A.M. 2009. The ß 2-tubulin gene from three tephritid fruit fly species and use of its promoter for sperm marking. Insect Biochemistry and Molecular Biology. 30:508-515.
Schetelig, M.F., Scolari, F., Handler, A.M., Kittelmann, S., Gasperi, G., Wimmer, E.A. 2009. Site-specific recombination for the modification of transgenic strains of the Mediterranean fruit fly Ceratitis capitata. Proceedings of the National Academy of Sciences. 106(43):18171-18176.
Pereira, R., Sivinski, J.M., Teal, P.E. 2010. Influence of a juvenile hormone analog and dietary protein on male Anastrepha suspensa (Diptera:Tephritidae) sexual success. Journal of Economic Entomology. 103(1): 40-46.
Cancino, J., Ruiz, L., Lopez, P., Sivinski, J.M. 2009. The suitability of Anastrepha spp. and Ceratitis capitata larvae as hosts of Diachasmimorpha longicaudata and Diachasmimorpha tryoni: Effects of host age and radiation dose and implications for quality control in mass rearing. Biocontrol Science and Technology. 19:81-94.
Palenchar, J., Holler, T., Moses-Rowley, A., Mcgovern, R., Sivinski, J.M. 2009. Evaluating the use of irradiated caribean fruit fly (Diptera:Tephritidae) larvae for laboratory rearing of Doryctobracon areolatus (Hymenoptera: Braconidae). Florida Entomologist. 92(4)535-537.
Mankin, R.W., Moore, M. 2010. Acoustic detection of Oryctes rhinoceros (Coleoptera: Scarabaeidae: Dynastinae) and Nasutitermes luzonicus (Isoptera: Termitidae) in palm trees of urban Guam. Journal of Economic Entomology. 103:1135-1143.
Pereira, R., Sivinski, J.M., Teal, P.E., Brockmann, J. 2010. Enhancing male sexual success in a lekking fly (Ananstrepha suspensa (Diptera: Tephritidae) through a juvenile hormone analog has no effect on adult mortality. Journal of Insect Physiology. 56:1552-1557.
Aluja, M., Ovruski, S.M., Guillen, L., Orono, L.E., Sivinski, J.M. 2009. Comparison of the host searching and oviposition behaviors of the tephtitid (Diptera) parasitoids Aganaspis pelleranoi and Odontosema anastrephae (Hymenoptera: Figitidae, Eucoilinae). Journal of Insect Behavior. 22:423-451.
Meagher Jr, R.L., Nagoshi, R.N. 2010. Identification of fall armyworm (Lepidoptera:Noctuidae) host strains using male-derived spermatophores. Florida Entomologist. 93(2):191-197.
Nagoshi, R.N., Adamczyk Jr, J.J., Meagher Jr, R.L., Gore, J., Jackson, R.E. 2007. Using stable isotope analysis to examine the fall armyworm (Lepidoptera: Noctuidae) host strains in a cotton habitat. Journal of Economic Entomology. 100(5):1569-1576.
Mankin, R.W., Samson, P.R., Chandler, K.J. 2009. Acoustic detection of Melolonthine larvae in Australian sugarcane. Journal of Economic Entomology. 102:1523-1535.
Wenninger, E., Hall, D.G., Mankin, R.W. 2009. Vibrational communication between the sexes in Diaphorina citri (Hemiptera: Psyllidae). Annals of the Entomological Society of America. 102:547-555.
Mankin, R.W., Anderson, J.B., Mizrach, A., Epsky, N.D., Shuman, D., Heath, R.R., Mazor, M., Hetzroni, A., Grinspun, J., Taylor, P.W., Garrett, S.L. 2004. Broadcasts of wing-fanning vibrations recorded from calling male Ceratitis capitata (Wiedemann) (Diptera: Tephritidae) increase captures of females in traps. Journal of Economic Entomology. 97:1229-1309.
Mankin, R.W., Benshemesh, J. Geophone detection of subterranean termite and ant activity. Journal of Economic Entomology. 2006. 99(1):244-250.
Mankin, R.W. 2006. Increase in acoustic detectability of Plodia interpunctella larvae after low-engergy microwave radar exposure. Florida Entomologist. 89:416-418.
Shirk, P.D., Bossin, H., Furlong, R.B., Gillett, J.L. 2007. Regulation of Junonia coenia Densovirus P9 Promoter Expression. Insect Molecular Biology.
Schetelig, M.F., Horn, C., Handler, A.M., Wimmer, E.A. 2007. Development of an embryonic lethality system for transgenic sit in the fruit pest, ceratitis capitata. IAEA-FAO Area Wide Insect Management Symposium Proceedings. 85-93.
Schetelig, M.F., Scolari, F., Handler, A.M., Gasperi, G., Wimmer, E.A. 2009. New genetic tools for improving SIT in Ceratitis capitata: embryonic lethality and sperm marking. Fruit Flies of Economic Importance International Symposium. 299-305.
Leopold, R.A., Rajamohan, A., Shelly, T.E., Handler, A.M. 2010. Quality Testing of Three Species of Tephritid Fruit Flies After Embryo Cryopreservation. Annals of the Entomological Society of America. 103(2):264-272.
Meagher Jr, R.L., Landolt, P.J. 2010. Binary floral lure attractive to velvetbean caterpillar adults (Lepidoptera: Noctuidae). Florida Entomologist. 93(1):73-79
Copeland, C.S., Hoy, M.A., Jeyaprakash, A., Aluja, M., Ramirez-Romero, R., Sivinski, J.M. 2010. Genetic characteristics of bisexual and female-only populations of Odontosema anastrephae (Hymenoptera:Figitidae). Florida Entomologist. 93(3):437-443.