ENVIRONMENTAL AND GENETIC FACTORS AFFECTING PATHOGEN PERSISTENCE IN ANIMAL WASTE AND TRANSFER TO CROPS
Project Number: 5325-32000-006-00
Start Date: Mar 15, 2006
End Date: Mar 14, 2011
1: The identification of factors contributing to the persistence and horizontal transfer of MAP and other pathogens on dairy farms. We will examine the role alternate protozoan hosts play in MAP persistence and horizontal transfer. We will also perform functional genomic analysis of MAP in the environment to elucidate clues about the strategies employed by this organism for environmental survival.
2: The development of methodologies for the reduction of pathogens in waste. Two of the most common waste treatment methodologies are aerobic and anaerobic digestion, but little is known about the effect these processes have on the microbial population structure, pathogen levels, and volatile compound missions. The bacterial community structure, chemical composition and volatile compounds emitted from animal waste, aerobic and anaerobic digesters, and holding tanks receiving effluent will be characterized. We will also analyze the effect of diet and dietary supplements on the microbial population structure, pathogen levels, and volatile compound emissions from animal waste and animal waste treatment systems.
3: Pathogen transfer from manure and CAFOs (confined animal feeding operations) to crops. We will use high volume aerosol collection and agar impaction devices to collect bacteria from aerosols near CAFOs. Aerosol samples will be evaluated for pathogens using culture and non-culture based methods. We will examine the bacterial community structure and pathogen levels on crops fertilized with waste using culture and non-culture based methods and compare them to those of plants fertilized with only chemical fertilizers. We will study the colonization of crop plants by Salmonella enterica grown in soil amended with manure spiked with pathogens using standard culture and quantitative PCR methods.
The goal of this project is to reduce the incidence of foodborne illnesses by reducing the levels of pathogens in animal waste and in CAFO environments. In the next five years we will identify the locations in CAFOs that serve as reservoirs for pathogenic bacteria such as MAP, develop low cost pathogen abatement strategies for contaminated animal waste, examine the effect of diet on pathogen load and evaluate the risk of pathogen transfer from CAFOs to surrounding crop plants via aerosols and from fertilizing crop plants with contaminated manure. FORMERLY: 5325-32000-002-00D; 5325-42000-023-00D. 5325-42000-028-00D combined into this project (4/04). Replaces 5325-32000-005-00D (2/06). Combining to 5325-42000-043-00D, 5325-41000-035-00D, 5325-42000-044-00D (1/09).