Start Date: May 20, 2005
End Date: May 19, 2010
Research will be conducted using field and greenhouse chambers for control of ozone and carbon dioxide concentrations and temperature. Effects on yield, development, and quality for specific crops (e.g. soybean, snap bean, wheat and selected forages) and on changes in soil carbon will be tested. To evaluate the interactive effects of ozone, carbon dioxide, and temperature on plants, studies will include measurements of leaf conductance, photosynthesis, chlorophyll fluorescence, carbon metabolism, and biochemical systems that detoxify reactive oxygen intermediates formed from ozone. To evaluate the effects on agricultural soils, studies of plant response to ozone and carbon dioxide will be accompanied by measurements of soil microbial biomass, microbial community structure, and microbial activity, soil carbon distribution among carbon pools, soil carbon turnover using delta 13C mass spectrometry, and nitrogen mineralization. Genetic variability of response to ozone and carbon dioxide will be assessed through a combination of screening and pedigree analysis. Development and testing of Arabidopsis mutants will be conducted to identify genes associated with plant response to ozone.