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Research Project:
EVOLUTIONARY ENZYMES AND SEPARATION PROCESSES FOR IMPROVED BIOREFINING OF CROPS AND RESIDUES
Location: Bioproduct Chemistry and Engineering Research
Publications
(Clicking on the reprint icon
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Ethanol in biorefining and dehydration of agricultural materials: energy, capital cost, and product quality implications
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| Robertson, G.H., Offeman, R.D., Cao, T., Orts, W.J. 2011. Ethanol in biorefining and dehydration of agricultural materials: energy, capital cost, and product quality implications. Biofuels, Bioproducts, & Biorefining (Biofpr). 5:37-53. |
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Functional characterization of two distinct xyoglucanases from rumenal microbes
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| Wong, D., Chan, V.J., Mccormack, A.A., Batt Throne, S.B. Functional characterization of two distinct xyoglucanases from rumenal microbes. Meeting Abstract, AGFD 125. |
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and applications of microbial ß-D-xylosidases featuring the catalytically efficient enzyme from Selenomonas ruminantium
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| Jordan, D.B., Wagschal, K.C. 2010. Properties and applications of microbial beta-D-xylosidases. Applied Microbiology and Biotechnology. 86(6):1647-1658 |
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Cloning and characterization of alpha-glucuronidase enzymes from mixed cultures
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| Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzymes from mixed cultures. Meeting Abstract. 85. |
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Extraction of ethanol with higher carboxylic acid solvents and their toxicity to yeast
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| Offeman, R.D., Franquivillanueva, D.M., Cline, J.L., Robertson, G.H., Orts, W.J. 2010. Extraction of ethanol with higher carboxylic acid solvents and their toxicity to yeast. Separation and Purification Technology. 72: 180-185. |
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Development and validation of a hydrophilic interaction liquid chromatographic method for determination of aromatic amines in environmental water
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| Li, R., Zhang, Y., Lee, C.C., Lu, R., Huang, Y. Development and validation of a hydrophilic interaction liquid chromatographic method for determination of aromatic amines in environmental water. Journal of Chromatography, 2010. A 1217(11), pp 1799-1805. |
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Cloning and characterization of an exo-xylglucanase from rumenal microbial metagenome
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| Wong, D., Chan, V.J., Mccormack, A.A., Batt Throne, S.B. 2010. Cloning and characterization of an exo-xylglucanase from rumenal microbial metagenome. Protein and Peptide Letters. 17:803-808. |
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Cloning and characterization of alpha-glucuronidase enzyme
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| Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Wong, D., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzyme. Meeting Abstract. |
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Cloning and characterization of alpha-glucuronidase enzyme
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| Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Wong, D., Orts, W.J. 2010. Cloning and characterization of alpha-glucuronidase enzyme. Meeting Abstract. |
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Expression and secretion of cellulosmal enzymes by engineered Saccharomyces cerevisiae
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| Batt Throne, S.B., Wan, D., Doi, R.H., Wong, D. 2010. Expression and secretion of cellulosmal enzymes by engineered Saccharomyces cerevisiae. Meeting Abstract, 2010; CELL Poster Session; Poster #57 (Paper ID 18330). |
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Chromosomal integration of recombinant alpha-amylase and glucoamylase genes in saccharomyces cerevisiae for starch conversion
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| Wong, D., Batt Throne, S.B., Robertson, G.H., Lee, C.C., Wagschal, K.C. 2010. Chromosomal integration of recombinant alpha-amylase and glucoamylase genes in saccharomyces cerevisiae for starch conversion. Industrial Biotechnology. 6:112-118. |
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Engineering lower inhibitor affinities in beta-D-xylosidase by site-directed mutagenesis of Trp 145
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| Jordan, D.B., Wagschal, K.C., Fan, Z., Yuan, L., Braker, J.D., Heng, C. 2010. Engineering lower inhibitor affinities in beta-D-xylosidase by site-directed mutagenesis of Trp 145. Meeting Abstract. 84. |
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A novel xyloglucan-specific endo-beta-1,4-glucanase: biochemical properties and inhibition studies
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| Wong, D., Chan, V.J., Mccormack, A.A., Batt Throne, S.B. 2010. A novel xyloglucan-specific endo-beta-1,4-glucanase: biochemical properties and inhibition studies. Applied Microbiology and Biotechnology. 86:1463-1471. |
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Engineering Lower Inhibitor Affinities in Beta-D-Xylosidase
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| Fan, Z., Yuan, L., Jordan, D.B., Wagschal, K.C., Heng, C., Braker, J.D. 2010. Engineering lower inhibitor affinities in beta-D-xylosidase. Applied Microbiology and Biotechnology. 86(4):1099-1113. |
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Cloning and characterization of an alpha-glucuronidase from a mixed microbial population
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| Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Robertson, G.H., Orts, W.J. 2009. Cloning and characterization of an alpha-glucuronidase from a mixed microbial population. Enzyme and Microbial Technology. 155(1-3):314-320. |
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Molecular cloning and characterization of multidomain xylanase from manure library
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| Li, R., Kibblewhite, R.E., Orts, W.J., Lee, C.C. 2009. Molecular cloning and characterization of multidomain xylanase from manure library. World Journal of Microbiology and Biotechnology. doi:10:1007/s11274-009-011106 |
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Engineer Sccharomyces cerevisiae for consolidated bioprocessing
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| Wong, D., Batt Throne, S.B., Wan, D., Lee, C.C., Wagschal, K.C. 2009. Engineer Sccharomyces cerevisiae for consolidated bioprocessing. Meeting Abstract. |
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Applications of metagenomics for industrial bioproducts
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| Wong, D. 2010. Applications of metagenomics for industrial bioproducts. Wong, D. Applications of metagenomics for industrial bioproducts. In: Marco, D., editor. Metagenomics: Theory, methods and applications. Norfolk, UK. Caister Academic Press.p. 141-158. |
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Cloning biomass-degrading enzymes from environmental sources
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| Lee, C.C., Wong, D., Wagschal, K.C., Orts, W.J. 2009. Cloning biomass-degrading enzymes from environmental sources. Meeting Abstract. |
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Purification and characterization of a glycoside hydrolase family 43 Beta-xylosidase from Geobacillus thermoleovorans IT-08
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| Wagschal, K.C., Heng, C., Lee, C.C., Robertson, G.H., Orts, W.J., Wong, D. 2008. Purification and characterization of a glycoside hydrolase family 43 Beta-xylosidase from Geobacillus thermoleovorans IT-08. Applied Biochemistry and Biotechnology. 155(1-3):1-10. |
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Biochemical characterization of a novel dual-function a-L-arabinofuranosidase/b-xylosidase isolated from a compost starter mixture
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| Wagschal, K.C., Heng, C., Lee, C.C., Wong, D. 2009. Biochemical characterization of a novel dual-function a-L-arabinofuranosidase/b-xylosidase isolated from a compost starter mixture. Applied Microbiology and Biotechnology. 81:855-863. |
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Metagenomic gene discovery and enzyme engineering for biomass conversion
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| Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. Metagenomic gene discovery and enzyme engineering for biomass conversion. American Chemical Society Western Regional Meeting. American Chemical Society Symposium: New Developments in Biofuels Research. Abstract 204. |
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Gene discovery and engineering for biomass degradation
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| Lee, C.C., Wagschal, K.C., Wong, D. 2008. Gene discovery and engineering for biomass degradation. United States-Japan Cooperative Program in Natural Resources. 2008. 50-51. |
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Structure and Action Mechanism of Ligninolytic Enzymes
- (Review Article)
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| Wong, D. 2009. Structure and Action Mechanism of Ligninolytic Enzymes. Applied Biochemistry and Biotechnology. 157(2):174-209. |
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Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme
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| Lee, C.C., Accinelli, R., Smith, M.R., Wagschal, K.C., Orts, W.J., Wong, D. 2008. Cloning of Bacillus licheniformis xylanase gene and characterization of recombinant enzyme. Current Microbiology. 57:301-305. |
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Cloning, Expression and Characterization of a Glycoside Hydrolase Family 39 Xylosidase from Bacillus Halodurans C-125
- (Peer Reviewed Journal)
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| Wagschal, K.C., Franquivillanueva, D.M., Lee, C.C., Robertson, G.H., Wong, D. Cloning, Expression and Characterization of a Glycoside Hydrolase Family 39 Xylosidase from Bacillus Halodurans C-125. Applied Biochemistry and Biotechnology. |
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Cloning and characterization of xylanase gene from hot spring microorganism
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| Lee, C.C., Accinelli, R., Smith, M.R., Wagschal, K.C., Orts, W.J., Wong, D. Cloning and characterization of xylanase gene from hot spring microorganism. Meeting Abstract #307. |
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Enzymatic Deconstruction of Backbone Structures of the Ramified Regions in Pectins
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| Wong, D. 2007. Enzymatic Deconstruction of Backbone Structures of the Ramified Regions in Pectins. Protein Journal. 27(1):30-42. |
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Thermally Stable Amylases from Antarctic Psychrophilic Bacteria
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| (16-Feb-07) |
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Developing enzyme systems for biomass deconstruction
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| Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. 2007. Developing enzyme systems for biomass deconstruction. Meeting Abstract. Paper No. 217 |
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SATB1 Packages Densely Looped, Transcriptionally Active Chromatin for Coordinated Expression of Cytokine Genes
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| Cai, S., Lee, C.C., Kohwi-Shigematsu, T. 2006. Satb1 packages densely looped, transcriptionally active chromatin for coordinated expression of cytokine genes. Nature Genetics. (38):1278-1288 |
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EXPRESSION AND SECRETION OF CLOSTRIDIUM CELLULOVORANS XYNA IN SACCHAROMYCES CEREVISIAE
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| Batt, S.B., Wong, D., Wagschal, K.C., Lee, C.C. 2006. Expression and secretion of clostridium cellulovorans xyna in saccharomyces cerevisiae. Division of Biological Chemistry, Enzymes Program. Abstract 220. |
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GENETIC AND BIOCHEMICAL CHARACTERIZATION OF A NOVEL BACTERIAL A-L-ARABINOFURANOSIDASE
- (Peer Reviewed Journal)
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| Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Accinelli, R., Robertson, G.H., Wong, D. 2007. Genetic and biochemical characterization of a novel bacterial a-l-arabinofuranosidase. Enzyme and Microbial Technology. (40):747-753. |
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EVOLUTIONARY ENZYME DESIGN FOR IMPROVED BIOREFINING OF CROPS AND RESIDUES
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| Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. 2006. Evolutionary enzyme design for improved biorefining of crops and residues. Technical Program (abstract). P8. |
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SYNERGISTIC ACTION OF BARLEY ALPHA-AMYLASE AND LENTINULA EDODES GLUCOAMYLASE ON RAW STARCH HYDROLYSIS
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| Wong, D., Robertson, G.H., Lee, C.C., Wagschal, K.C. 2006. Synergistic action of barley alpha-amylase and lentinula edodes glucoamylase on raw starch hydrolysis. Approved 10/27/2005. American Society of Biochemistry & Molecular Biology, San Francisco, CA. April 1-4, 2006, Program 73.16, vol A27. |
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FERULOYL ESTERASE - A KEY ENZYME IN BIOMASS DEGRADATION
- (Peer Reviewed Journal)
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| Wong, D. Feruloyl esterase - a key enzyme in biomass degradation. 2005. Applied Biochemistry and Biotechnology, 133:87-112. |
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CLONING AND CHARACTERIZATION OF COLD-ACTIVE XYLANASE ENZYMES
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| Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of cold-active xylanase enzymes. 28th Symposium on Biotechnology for Fuels and Chemicals. Paper No. 1A-41. |
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CLONING AND CHARACTERIZATION OF A COLD ACTIVE XYLANASE ENZYME
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| Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of a cold active xylanase enzyme. Extremophiles. 10:295-300. |
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NATIVE OR RAW STARCH DIGESTION
- (Review Article)
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| Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Orts, W.J. 2006. Native or raw starch digestion: A Key step in energy efficient biorefining of grain. Journal of Agricultural and Food Chemistry, 54:353-65. |
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FERULOYL ESTERASE - A KEY ENZYME IN BIOMASS DEGRADATION
- (Review Article)
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| Wong, D. 2005. Feruloyl esterase - a key enzyme in biomass degradation. Applied Biochemistry and Biotechnology. 133:87-112. |
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CHARACTERIZATION AND DIRECTED EVOLUTION OF HEMICELLULOTIC ENZYMES USING NATURAL XYLOOLIGOACCHARIDE SUBSTRATES
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| Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Robertson, G.H., Wong, D.W.S. 2005. Characterization of hemicellulotic degrading enzymes using natural substrates. Symposium on Biotechnology for Fuels and Chemicals, Denver, CO. Paper No. 1B-65. Abstract A10, Enzyme Engineering XVIII. |
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