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United States Department of Agriculture

Agricultural Research Service

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Location: Animal Metabolism-Agricultural Chemicals Research

2009 Annual Report

1a.Objectives (from AD-416)
Determine the activity and/or concentration of hormones and ag chemicals in animal waste under various manure handling conditions in order to develop practices that minimize potential environmental impact.

1b.Approach (from AD-416)
This will be a joint effort between VPI and SU, who will be responsible for setting up and conducting the experimental trails, and ARS who will develop methodology and perform analyses of samples. Both partners will collaborate in the design of the experiments and interpretation of the results.

3.Progress Report

This cooperative study is essentially completed except for the chemical analysis of bacitracin in animal wastes. A microbial-based method for the detection of bacitracin in water extracts has been completed. Bacitracin is an antibiotic commonly used as a feed additive in the poultry industry. Although extracts of excreta from bacitracin-treated birds contained antibiotic activity in >90% of the pens assayed (but in none of the control pens) water-extractable residues of the bedding were typically non detectable and were similar for both control and antibiotic-fed animals. To date, results of this study suggest minimal environmental impacts emanating from field-application of poultry litter from bacitracin treated birds. Standards and samples were sent to a cooperator’s laboratory in 2007 for independent analyses, but the collaborators have not completed the analyses to date. Efforts are underway to complete the study by developing a method to determine bacitracin via a liquid chromatography-mass spectrometry. We plan to compare results of the relatively inexpensive bioassay with results obtained from the more expensive chemical assay.

ADODR monitoring activites include regular conference calls, and emails between parties are utilized for updates on the status of the cooperative agreement activities.

Last Modified: 9/22/2014
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