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United States Department of Agriculture

Agricultural Research Service

Research Project: MANIPULATION OF ARTHROPOD BEHAVIOR FOR PROTECTION OF HUMANS
2007 Annual Report


1a.Objectives (from AD-416)
Objective 1: Discover and develop next generation topical skin repellents, spatial repellents, contact irritants, and toxicants against mosquitoes and other arthropod disease vectors. Research toward this objective will primarily involve a consortium of military, government, academic, and private industry research groups using high throughput bioassays to test model compounds selected via computational chemistry from the large chemical library maintained by the BASF Corporation.

Objective 2: Develop new technology for preparation of fabrics having insect controlling and/or repelling properties. Repellent compounds will be covalently bonded to fabric polymers, and the resulting polymers will be tested to determine if repellent activity against mosquitoes is retained; if these derivatized polymers are repellent, then research will continue to optimize conditions required to produce insect repellent fabrics. Objective 3: Identify the mechanisms underlying the activity of key mosquito repellents and attractants, and develop ways to exploit the mechanisms for commercial use. This objective will focus on state-of-the-art application of odorant binding protein (OBP) research by covalently bonding cloned mosquito OBPs to HPLC columns for use in screening natural product and synthetic chemical libraries for binding activity. Bound compounds will be identified and tested in conventional behavioral bioassays for activity against mosquitoes. Also, the mechanisms of action for selected compounds will be investigated electrophysiologically, including single cell recording experimentation to pinpoint the sites of receptors involved in the perception of bioactive compounds.


1b.Approach (from AD-416)
Discover and develop next generation topical skin repellents, spatial repellents, contact irritants, and toxicants against mosquitoes and other arthropod disease vectors.

Develop new technology for preparation of fabrics having insect controlling and/or repelling properties.

Identify the mechanisms underlying the activity of key mosquito repellents and attractants, and develop ways to exploit the mechanisms for commercial use.


3.Progress Report
Behavioral assays to quantitatively measure mosquito responses to stimuli involved in host seeking and feeding were developed in the laboratory.


4.Accomplishments
New mosquito and tick repellent. There is a need for novel arthropod repellents with new chemical composition. A patent, Methods For Preparing Isolongifolenone And Its Use In Repelling Arthropods, has been applied for. It is expected that isolongifolenone will become available in large quantities for use in the perfume and arthropod repellent industries. National Program Component: NP104, goal 4.1.3 (Identify and test new classes of topical and area repellents for previously synthesized or natural volatiles.) Oviposition attractants for the arthropod disease vectors -The accomplishment concerns identification and patenting of a new mosquito oviposition attractant. There is a need for commercially viable oviposition attractants to utilize attract and kill technology in disease vector control. A patent, Novel biorational oviposition attractants to disease vector mosquitoes, has been applied for. It is expected that newly developed oviposition attractants would be used in strategies to curb the mosquito surveillance as well as control. National Program Component: NP104, goal 2.2.3 (Identify and synthesize host specific attractants for use in traps).


5.Significant Activities that Support Special Target Populations
None


6.Technology Transfer

Number of new CRADAs and MTAs3
Number of active CRADAs and MTAs6
Number of invention disclosures submitted3
Number of patent applications filed2
Number of non-peer reviewed presentations and proceedings7

Review Publications
Klun, J.A., Khrimian, A., Rowton, E., Kramer, M.H., Debboun, M. 2006. Biting-deterrent activity of a deet analog, two depa analogs and ss220 applied topically to human volunteers compared with deet against three species of blood-feeding flies. Journal of Medical Entomology. 43:1248-1251.

Tran, K., Chauhan, K.R. 2007. Structural activity of bovidic acid and related compounds as feeding deterrents against Aedes aegypti. Biopestic. Int. 3(1):53-57.

Chauhan, K.R., Raina, A. 2007. Effect of catnip oil and its major components on the Formosan subterranean termites. Biopesticides International 2(2):137-143.

Zhu, J., Zeng, X., Ma, Y., Liu, T., Qian, K., Han, Y., Xue, S., Tucker, B., Schultz, G., Coats, J. 2006. Comparisons of adult repellency and larvicidal activity of plant essential oils against mosquitoes. Journal of the Mosquito Control Association. 35:249-257

Carroll, J.F., Cantrell, C.L., Klun, J.A., Kramer, M.H. 2007. Repellency of two terpenoid compounds isolated from Callicarpa americana (Lamiaceae) against Ixodes scapularis and Amblyomma americanum ticks. Experimental and Applied Acarology. 41:215-224.

Last Modified: 10/1/2014
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