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United States Department of Agriculture

Agricultural Research Service

Research Project: MITIGATION OF OFF-FLAVORS IN CATFISH AQUACULTURE Project Number: 6435-43440-041-00
Project Type: Appropriated

Start Date: Dec 22, 2004
End Date: Dec 13, 2009

Objective:
1) Identify and characterize viruses that kill specific off-flavor producing cyanobacteria in aquaculture systems; 2) Identify, characterize, and develop novel bacterial strains that metabolize (biodegrade) geosmin and 2- methylisoborneol, and develop them for use in eliminating those chemicals from aquaculture systems; and 3) Identify and characterize genes and enzymes involved in the biosynthesis of off-flavor metabolites in aquaculture systems. Build on an understanding of the mechanisms used in regulating gene expression and in catalysis of individual reactions, and develop methods to control the production of geosmin and MIB.

Approach:
The cyanophage community in catfish ponds will be screened and its members and their cyanobacterial host strains will be identified. Virulent cyanophage that kill specific cyanobacteria (lytic) will be identified and isolated from the environment, and the kinetics of phage replication and cyanobacterial population reduction will be determined. Bacteria will be isolated and characterized regarding ability to degrade geosmin or MIB at relevant concentrations, metabolic pathways employed and metabolites produced, mechanisms for controlling geosmin and MIB degradative enzyme biosynthesis, and taxonomy. Towards development of methods for controlling off-flavor production, the genes and enzymes of the biosynthetic pathway of MIB and geosmin will be characterized.

Last Modified: 11/22/2014
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