Start Date: Dec 22, 2004
End Date: Dec 13, 2009
The cyanophage community in catfish ponds will be screened and its members and their cyanobacterial host strains will be identified. Virulent cyanophage that kill specific cyanobacteria (lytic) will be identified and isolated from the environment, and the kinetics of phage replication and cyanobacterial population reduction will be determined. Bacteria will be isolated and characterized regarding ability to degrade geosmin or MIB at relevant concentrations, metabolic pathways employed and metabolites produced, mechanisms for controlling geosmin and MIB degradative enzyme biosynthesis, and taxonomy. Towards development of methods for controlling off-flavor production, the genes and enzymes of the biosynthetic pathway of MIB and geosmin will be characterized.