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United States Department of Agriculture

Agricultural Research Service

Research Project: MICROBIAL CATALYSTS TO PRODUCE FUEL ETHANOL AND VALUE ADDED PRODUCTS Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)

Chapter 3: Biofuel Demand Realization - (Book / Chapter)
Hughes, S.R., Qureshi, N. 2010. Biofuel demand realization. In: Vertes, A., Qureshi, N., Blascheck, H.P., Yukawa, H., editors. Biomass to Biofuels: Strategies to Global Industries. UK:John Wiley & Sons Limited. p. 55-69.
Functional Expression of the Thiolase Gene thl from Clostridium beijerinckii p260 in Lactococcus lactis and Lactobacillus buchneri - (Peer Reviewed Journal)
Liu, S., Bischoff, K.M., Qureshi, N., Hughes, S.R., Rich, J.O. 2010. Functional expression of the thiolase gene THl from Clostridium beijerinckii P260 in Lactococcus lactis and Lactobacillus buchneri. New Biotechnology. 27(4):283-288.
Fermentation of Corn Fiber Hydrolysate to Lactic Acid by the Moderate Thermophile Bacillus coagulans - (Peer Reviewed Journal)
Bischoff, K.M., Liu, S., Hughes, S.R., Rich, J.O. 2010. Fermentation of corn fiber hydrolysate to lactic acid by the moderate thermophile Bacillus coagulans. Biotechnology Letters. 32:823-828.
Production of Value-added Products by Lactic Acid Bacteria - (Book / Chapter)
Liu, S., Li, Y., Azaizeh, H., Cui, F., Tafesh, A., Bischoff, K.M. 2010. Production of value-added products by lactic acid bacteria. In: Hou, C.T., Shaw, J.-F., editors. Biocatalysis and Molecular Engineering. New York: John Wiley and Sons. p. 421-435.
Enhanced Production of Carboxylic Acids by Engineering of Rhizopus - (Abstract Only)
Skory, C.D., Hector, R.E., Gorsich, S.W., Rich, J.O. 2009. Enhanced Production of Carboxylic Acids by Engineering of Rhizopus [abstract].
Cloning and Expression of Laccase from Trametes versicolor in Saccharomyces cerevisiae using a Novel Vector System - (Abstract Only)
Pinkelman, R.J., Hughes, S.R., Choi, H., Bang, S.S. 2009. Cloning and expression of Laccase from Trametes versicolor in Saccharomyces cerevisiae using a novel vector system [abstract]. American Institute for Chemical Engineers. Talk 1. p. 1.
Production of the Anaerobic GMAX-L Yeast Using High-Throughput Mating and Transformation of Saccharomyces cerevisiae With Identified Genes For Simultaneous Cellulosic Ethanol and Biodiesel Production - (Abstract Only)
Hughes, S.R., Rich, J.O., Moser, B.R., Doll, K.M., Bischoff, K.M., Qureshi, N., Jones, M., Tasaki, K. 2009. Production of the anaerobic GMAX-L yeast using high-throughput mating and transformation of Saccharomyces cerevisiae with identified genes For simultaneous cellulosic ethanol and biodiesel production [abstract]. Bradley Biology Chemisty Seminar Series.
Lactic Acid Bacteria – Friend or Foe? Lactic Acid Bacteria in the Production of Polysaccharides and Fuel Ethanol     Reprint Icon - (Peer Reviewed Journal)
Rich, J.O., Bischoff, K.M., Leathers, T.D., Cote, G.L., Liu, S. 2010. Lactic acid bacteria – Friend or Foe? Lactic acid bacteria in the production of Polysaccharides and fuel ethanol. KKU Research Journal. 15(5):424-435.
Genetic Engineering of Rhizopus for Enhancing Lactic Acid Production - (Abstract Only)
Rich, J.O., Skory, C.D. 2009. Genetic Engineering of Rhizopus for enhancing lactic acid production [abstract]. Conference on Fermentation Technology for Value Added Agricultural Products. Paper Fer406. p. 25.
Lactic Acid Bateria - Friend or Foe? Lactic Acid Bacteria in the Production of Polysaccharides and Fuel Ethanol - (Abstract Only)
Rich, J.O., Liu, S., Leathers, T.D., Bischoff, K.M. 2009. Lactic acid bacteria - Friend or Foe? Lactic acid bacteria in the production of polysaccharides and fuel ethanol [abstract]. Conference on Lactic Acid Bacteria in Thai Food and Feed Industries. Paper L01. p. 69.
How Microbes Tolerate Ethanol and Butanol - (Review Article)
Liu, S., Qureshi, N. 2009. How microbes tolerate ethanol and butanol. New Biotechnology. 26(3/4):117-121.
Development of Thermotolerant Consolidated Bioprocessing Microbes for Cost-efficient Cellulosic Ethanol Production - (Abstract Only)
Christopher, L.P., Gibbons, W., Bleakeley, B., West, T., Hughes, S.R., Sani, R.K., Bang, S.S. 2009. Development of thermotolerant consolidated bioprocessing microbes for cost-efficient cellulosic ethanol production [abstract]. Industrial Biotechnology and Bioprocessing. Talk #4. p. 1.
Industrial Saccharomyces cerevisiae Yeast Strain Engineered to Convert Glucose, Mannose, Arabinose, and Xylose (GMAX) to Ethanol Anaerobically - (Abstract Only)
Hughes, S.R., Qureshi, N., Butt, T., Bang, S., Bischoff, K.M., Saha, B.C., Liu, S., Rich, J.O. 2009. Industrial Saccharomyces cerevisiae yeast strain engineered to convert glucose, mannose, arabinose, and xylose (GMAX) to ethanol anaerobically [abstract]. American Institute of Chemical Engineers. Talk #2. p. 1.
Advanced Biorefineries for Production of Fuel Ethanol - (Book / Chapter)
Hughes, S.R., Gibbons, W., Kohl, S. 2010. Advanced biorefineries for production of fuel ethanol. In: Vertes, A., Qureshi, N., Blaschek, H., Yukawa, H., editors. Biomass in Biofuels: Strategies for Global Industries. Chapter 4. p. 71-88.
Gmax Yeast Background Strain Made from Industrial Tolerant Saccharomyces Cerevisiae Engineered to Convert Sucrose, Starch and Cellulosic Sugars Universally to Ethanol Anaerobically with Concurrent Coproduct Expression - (Abstract Only)
Hughes, S.R., Butt, T., Boiley, J., Bang, S., Gibbons, W., Bischoff, K.M. 2009. GMAX yeast background strain made from industrial tolerant Saccharomyces cerevisiae engineered to convert sucrose, starch and cellulosic sugars universally to ethanol anaerobically with concurrent coproduct expression [abstract]. Society for In vitro Biology. Abstract #106. p. 135.
Gmax-L Saccharomyces Cerevisiae Strains for Profitable Sustainable Cellulosic Ethanol and Biodiesel Production Concurrently Using Engineered Workcell - (Abstract Only)
Hughes, S.R., Moser, B.R., Doll, K.M., Tasaki, K., Harmsen, A., Jones, M., Bang, S., Gibbons, W., Butt, T. 2009. GMAX-L Saccharomyces cerevisiae strains for profitable sustainable cellulosic ethanol and biodiesel production concurrently using engineered workcell [abstract]. Center for Process Analytical Chemisty. Abstract No. 2. p. 1.
Characterization of Extremophilic Cellulose-Degrading Bacteria from the Deep Subsurface of the Homestake Gold Mine, Lead, South Dakota, USA - (Abstract Only)
Rastogi, G., Muppidi, G., Gurram, R., Adhikari, A., Bang, S., Christopher, L., Bischoff, K.M., Hughes, S.R., Apel, W., Sani, R.K. 2009. Characterization of extremophilic cellulose-degrading bacteria from the deep subsurface of the Homestake Gold Mine, Lead, South Dakota [abstract]. Symposium on Biotechnology for Fuels and Chemicals. Poster No. 6-11. p. 131.
Hemicellulolytic Enzymes from the Maize Endophyte Acremonium zeae - (Abstract Only)
Bischoff, K.M., Jordan, D.B., De Rezende, S.T., Rich, J.O. 2009. Hemicellulolytic enzymes from the maize endophyte Acremonium zeae [abstract]. Biotechnology for Fuels and Chemicals. Poster No. 5-57. p. 120.
Rapid evaluation of fuel ethanol contaminant biofilms - (Abstract Only)
Rich, J.O., Leathers, T.D., Bischoff, K.M. 2009. Rapid Evaluation of Fuel Ethanol Contaminant Biofilms [abstract]. 31st Symposium on Biotechnology for Fuels and Chemicals.
Gmax Yeast Background Strain Made from Industrial Tolerant Saccharomyces Cerevisiae Engineered to Convert Pretreated Lignocellulosic Starch and Cellulosic Sugars Universally to Ethanol Anaerobically - (Abstract Only)
Hughes, S.R., Tasaki, K., Moser, B., Doll, K., Butt, T., Sterner, D., Bischoff, K., Hector, R., Jones, M., and Bang, S. 2009. GMAX yeast background strain made from industrial tolerant Saccharomyces cerevisiae engineered to convert pretreated Lignocellulosic starch and cellulosic sugars universally to ethanol anaerobically [abstract]. Cambridge Healthtech Institute, Advanced Biofuels Development Summit. Abstract No. 2. p. 3.
Historical Perspective of Biofuels: Learning from the Past to Rediscover the Future - (Peer Reviewed Journal)
Songstad, D.D., Lakshmanan, P., Chen, J., Gibbons, W., Hughes, S.R., Nelson, R. 2009. Historical perspective of biofuels: Learning from the past to rediscover the future. In Vitro Cellular and Developmental Biology - Plants. 45:189-192.
Application of combinatorial biocatalysis for a unique ring expansion of dihydroxymethylzearalenone - (Peer Reviewed Journal)
Rich, J.O., Budde, C.L., Mcconeghey, L.D., Cotterill, I.C., Mozhaev, V.V., Singh, S.B., Goetz, M.A., Zhao, A., Michels, P.C., Khmelnitsky, Y.L. 2009. Application of combinatorial biocatalysis for a unique ring expansion of dihydroxymethylzearalenone. Bioorganic and Medicinal Chemistry Letters. 19:3059-3062.
Integrated Biorefineries with Engineered Microbes and High-value Co-products for Profitable Biofuels Production - (Review Article)
Gibbons, W.R., Hughes, S.R. 2009. Integrated biorefineries with engineered microbes and high-value co-products for profitable biofuels production. In Vitro Cellular and Developmental Biology - Plants. 45:218-228.
Automated Yeast Transformation Protocol to Engineer S. cerevisiae Strains for Cellulosic Ethanol Production with Open Reading Frames that Express Proteins Binding to Xylose Isomerase Identified using Robotic Two-hybrid Screen - (Peer Reviewed Journal)
Hughes, S.R., Rich, J.O., Bischoff, K.M., Hector, R.E., Qureshi, N., Saha, B.C., Dien, B.S., Liu, S., Jackson Jr, J.S., Sterner, D.E., Butt, T.R., Labaer, J., Cotta, M.A. 2009. Automated yeast transformation protocol to engineer S. cerevisiae strains for cellulosic ethanol production with open reading frames that express proteins binding to xylose isomerase identified using robotic two-hybrid screen. Journal of the Association for Laboratory Automation. 8:200-212.
Creating libraries for commercial yeast strains through miniaturization of cloning and transformations using the BioRAPTR FRD Microfluidic workstation - (Abstract Only)
Yoder, A., Pajak, L., Jackson Jr, J.S., Hughes, S.R. 2009. Creating libraries for commercial yeast strains through miniaturization of cloning and transformations using the BioRAPTR FRD microfluidic workstation [abstract]. LabAutomation. Poster #12. p. 49.
Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings - (Abstract Only)
Hughes, S.R., Doll, K., Moser, B., Bang, S., Rich, J., Qureshi, N., Hector, R., Dien, B., Cotta, M., Bischoff, K., Liu, S., Jones, M. 2009. Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings [abstract]. LabAutomation 2009. Abstract No. 109. p. 42.
Isolation and Characterization of Cellulose-degrading Bacteria from the Deep Subsurface of the Homestake Gold Mine, Lead, South Dakota, USA - (Peer Reviewed Journal)
Rastogi, G., Muppidi, G.L., Gurram, R.N., Adhikari, A., Bischoff, K.M., Hughes, S.R., Apel, W.A., Bang, S.S., Dixon, D.J., Sani, R.K. 2009. Isolation and characterization of cellulose-degrading bacteria from the deep subsurface of the Homestake Gold Mine, Lead, South Dakota, USA. Journal of Industrial Microbiology and Biotechnology. 36:585-598.
Automated Yeast Mating Protocol Using Open Reading Frames from Saccharomyces cerevisiae Genome to Improve Yeast Strains for Cellulosic Ethanol Production - (Peer Reviewed Journal)
Hughes, S.R., Hector, R.E., Rich, J.O., Qureshi, N., Bischoff, K.M., Dien, B.S., Saha, B.C., Liu, S., Jackson Jr, J.S., Sterner, D.E., Butt, T.R., Labaer, J., Cotta, M.A. 2009. Automated yeast mating protocol using open reading frames from Saccharomyces cerevisiae genome to improve yeast strains for cellulosic ethanol production. Journal of the Association for Laboratory Automation. 8:190-199.
Extracellular Hemicellulolytic Enzymes from the Maize Endophyte Acremonium zeae - (Peer Reviewed Journal)
Bischoff, K.M., Wicklow, D.T., Jordan, D.B., De Rezende, S.T., Liu, S., Hughes, S.R., Rich, J.O. 2009. Extracellular hemicellulolytic enzymes from the maize endophyte Acremonium zeae. Current Microbiology. 58:499-503.
Modeling Bacterial Contamination of Fuel Ethanol Fermentation - (Peer Reviewed Journal)
Bischoff, K.M., Liu, S., Leathers, T.D., Worthington, R.E., Rich, J.O. 2009. Modeling bacterial contamination of fuel ethanol fermentation. Biotechnology and Bioengineering. 103(1):117-122.
Make use of lactic acid bacteria in biomass to biofuel - (Abstract Only)
Liu, S., Bischoff, K.M., Leathers, T.D., Hughes, S.R., Rich, J.O. 2008. Make use of lactic acid bacteria in biomass to biofuel [abstract]. International Symposium on Biocatalysis and Biotechnology. Post No. M-2. p. 60.
Conversion of Biomass Hydrolysates and Other Substrates to Ethanol and Other Chemicals by Lactobacillus buchneri - (Peer Reviewed Journal)
Liu, S., Bischoff, K.M., Hughes, S.R., Leathers, T.D., Price, N.P., Qureshi, N., Rich, J.O. 2009. Conversion of biomass hydrolysates and other substrates to ethanol and other chemicals by Lactobacillus buchneri. Letters of Applied Microbiology. 48(3):337-342.
Conversion of Biomass to Ethanol by Other Organisms - (Book / Chapter)
Liu, S. 2009. Conversion of biomass to ethanol by other organisms. In: Vertes, A.A., Blascheck, H.P., Yukawa, H., Qureshi, N., editors. Biomass to Biofuels. Chapter 14. West Sussex, UK:John Wiley & Sons, Ltd. p. 293-310.
Three-vector System for use on Automated Systems for High-level Functional Expression of Value-added Co-products with Xylose Isomerase and Xylulokinase in an Industrial Saccharomyces cerevisiae Strain - (Abstract Only)
Rich, J.O., Hughes, S.R., Bischoff, K.M., Leathers, T.D. 2008. Three-vector system for use on automated systems for high-level functional expression of value-added co-products with Xylose Isomerase and Xylulokinase in an industrial Saccharomyces cerevisiae strain [abstract]. Indiana Council of Engineering Societies. Abstract 13. p. 4.
Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system - (Peer Reviewed Journal)
Hughes, S.R., Sterner, D.E., Bischoff, K.M., Hector, R.E., Dowd, P.F., Qureshi, N., Bang, S.S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Dien, B.S., Mertens, J.A., Caughey, R.J., Liu, S., Butt, T.R., Labaer, J., Cotta, M.A., Rich, J.O. 2009. Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system. Plasmid Journal. 61(1):22-38.
Improving Biocatalysts for the Production of Biofuels from Lignocellulosic Feedstocks - (Abstract Only)
Rich, J.O., Hughes, S.R., Bischoff, K.M., Leathers, T.D., Liu, S. 2008. Improving biocatalysts for the production of biofuels from Lignocellulosic feedstocks [abstract]. U.S. Japan Natural Resources Meeting. p. 54-55.
Cellulosic Ethanol Production from Saccharomyces cerevisiae Engineered for Anaerobic Conversion of Pretreated Lignocellulosic Sugars to Ethanol - (Abstract Only)
Rich, J.O., Bischoff, K.M., Hughes, S.R. 2008. Cellulosic ethanol production from Saccharomyces cerevisiae engineered for anaerobic conversion of pretreated Lignocellulosic sugars to ethanol [abstract]. American Chemical Society. Paper ID1211547.
Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars - (Abstract Only)
Hughes, S.R., Javers, J., Labaer, J., Butt, T., Hector, R.E., Dien, B.S., Saha, B.C., Bischoff, K.M., Kohl, S., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars [abstract]. Society of Industrial Microbiology. Session 34, S164, p. 106.
Thermophilic Hydrolytic Enzymes from the Maize Endophyte Acremonium zeae - (Abstract Only)
Bischoff, K.M. 2008. Thermophilic hydrolytic enzymes from the maize endophyte Acremonium zeae [abstract]. Society of Industrial Microbiology. Abstract No. S162. p. 105.
Lactobacillus buchneri: A novel biocatalyst for ethanol production - (Abstract Only)
Liu, S. 2008. Lactobacillus buchneri: A Novel Biocatalyst for Ethanol Production [abstract]. MWA Bioenergy Research Forum. Poster No. 14.
Proteomic Approach for Molecular Mechanisms under Ethanol Stress in Lactobacillus buchneri - (Abstract Only)
Liu, S., Bischoff, K.M., Leathers, T.D., Hughes, S.R., Rich, J.O. 2008. Proteomic approach for molecular mechanisms under ethanol stress in Lactobacillus buchneri [abstract]. American Society for Microbiology. 0-030. p. 155.
Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain - (Abstract Only)
Hughes, S.R., Jackson Jr, J.S., Dowd, P.F., Hector, R.E., Bischoff, K.M., Sterner, D., Bang, S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Li, X., Caughey, R.J., Liu, S., Skory, C.D., Butt, T., Labaer, J., Dien, B.S., Saha, B.C., Bowen, M., Qureshi, N., Rich, J.O., Leathers, T.D., Cotta, M.A., Farrelly, P., Slininger P.J. 2008. Three-vector system for high-level functional expression of value-added co-products with Xylose Isomerase and Xylulokinase in an industrial Saccharomyces cerevisiae strain [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit. Poster #1. p. 4.
Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic sugars anaerobically to ethanol - (Abstract Only)
Hughes, S.R., Bischoff, K.M., Hector, R.E., Dien, B.S., Saha, B.C., Javers, J., Gibbons, W., Bang, S., Nelson, R., Standish, K., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae engineered to convert pretreated Lignocellulosic sugars anaerobically to ethanol [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit, Seminar 6. p. 1.
Biofuels Group NSF DUSEL Project - (Abstract Only)
Bang, S., Sani, R., Bleakly, B., Bischoff, K.M., Hughes, S.R., Wagschal, K.C., Wong, D., Lee, C.C., Jordan, D.B., Pokkuluri, R., Schiffer, M. 2008. Biofuels Group NSF DUSEL Project [abstract]. Homestake DUSEL Spring Workshop. Talk 10. p. 2.
Ethanol production from modern biorefinery: Robotic platform for production of Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic sugars to ethanol anaerobically - (Abstract Only)
Hughes, S.R., Rich, J.O. 2008. Ethanol production from modern biorefinery: Robotic platform for production of Saccharomyces cerevisiae engineered to convert pretreated Lignocellulosic sugars to ethanol anaerobically [abstract]. Laboratory Robotics Information Group (LRIG). Seminar 1. p. 1.
Controlled Antibiotic use during Fuel Ethanol Production - (Abstract Only)
Bischoff, K.M., Scott, J., Leathers, T.D., Liu, S., Worthington, R. 2008. Controlled antibiotic use during fuel ethanol production [abstract]. National Science Foundation, Partnerships for Innovation Workshop. Poster No. 13.
Reversible derivatization to enhance enzymatic synthesis: Chemoenzymatic synthesis of Doxorubicin-14-O-Esters - (Peer Reviewed Journal)
Cotterill, I.C., Rich, J.O., Scholten, M.W., Mozhaeva, L., Michels, P.C. 2008. Reversible derivatization to enhance enzymatic synthesis: Chemoenzymatic synthesis of Doxorubicin-14-O-Esters. Biotechnology and Bioengineering. 101(3):435-440.
Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain - (Peer Reviewed Journal)
Hughes, S.R., Dowd, P.F., Hector, R.E., Panavas, T., Sterner, D.E., Qureshi, N., Bischoff, K.M., Bang, S.B., Mertens, J.A., Johnson, E.T., Li, X., Jackson Jr, J.S., Caughey, R.J., Riedmuller, S.B., Bartolett, S., Liu, S., Rich, J.O., Farrelly, P.J., Butt, T.R., Labaer, J., Cotta, M.A. 2008. Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain. Journal of Peptide Science. 14(9):1039-1050. Available: http://www3.interscience.wiley.com/cgi-bin/fulltext/119030240/PDFSTART.
Regulatory concerns associated with use of value-added recombinant proteins and peptides screened in hgh-throughput for expression in fuel ethanol yeast strains - (Abstract Only)
Hughes, S.R., Dowd, P.F., Hector, R.E., Bischoff, K.M., Rich, J.O., Cotta, M.A., Slininger, P.J., Rosentrater, K.A. 2008. Regulatory concerns associated with use of value-added recombinant proteins and peptides screened in hgh-throughput for expression in fuel ethanol yeast strains [abstract]. LabAutomation 2008, Track 5, Seminar 4. p. 5.
Carbohydrate utilization and detection of a nucleotide hydrolase in Lactobacillus buchneri - (Abstract Only)
Liu, S., Bischoff, K.M., Price, N.P., Hughes, S.R. 2007. Carbohydrate utilization and detection of a nucleotide hydrolase in Lactobacillus buchneri [abstract]. Symposium on Biocatalysis and Biotechnology. p. 49.
Regulatory steps associated with use of value-added recombinant proteins and peptides screened in high-throughput for expression in genetically engineered starch and cellulosic fuel ethanol yeast strains - (Abstract Only)
Hughes, S.R., Chen, H., Tsai, D., Thompson, W., Dowd, P.F., Hooberman, B., Benjamin, L., Alexander, L.J., Rosentrater, K.A., Rich, J.O. 2007. Regulatory steps associated with use of value-added recombinant proteins and peptides screened in high-throughput for expression in genetically engineered starch and cellulosic fuel ethanol yeast strains [abstract]. FDA Meeting. Talk 1.
Industrially useful microorganisms isolated from deep underground science and engineering laboratory (DUSEL) mine biofilms - (Abstract Only)
Hughes, S.R., Bang, S., Bleakley, B. 2007. Industrially useful microorganisms isolated from deep underground science and engineering laboratory (DUSEL) mine biofilms [abstract]. Meeting with South Dakota State Mine & Technology (SDSM&T) and South Dakota State University (SDSU). BioDUSEL Section, Day 2, Talk 3. p. 15.
Lactobacillus buchneri strain NRRL B-30929 converts a concentrated mixture of C5 and C6 sugars into ethanol and other products - (Peer Reviewed Journal)
Liu, S., Skinner-Nemec, K.A., Leathers, T.D. 2008. Lactobacillus buchneri strain NRRL B-30929 converts a concentrated mixture of C5 and C6 sugars into ethanol and other products. Journal of Industrial Microbiology and Biotechnology. 35:75-81.
Gram-positive bacteria as biocatalysts to convert biomass derived sugars into biofuel and chemicals - (Book / Chapter)
Liu, S., Cotta, M.A. 2007. Gram-positive bacteria as biocatalysts to convert biomass derived sugars into biofuel and chemicals. In: Hou, C.T., Shaw, J.-F., editors. Biocatalysis and Bioenergy. Chapter 14. New Jersey:John Wiley & Sons, Ltd. p. 294-264.
Antimicrobial susceptibility of Lactobacillus species isolated from commercial ethanol plants - (Peer Reviewed Journal)
Bischoff, K.M., Skinner-Nemec, K.A., Leathers, T.D. 2007. Antimicrobial susceptibility of Lactobacillus species isolated from commercial ethanol plants. Journal of Industrial Microbiology and Biotechnology. 34(11):739-744.
Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis - (Peer Reviewed Journal)
Liu, S., Dien, B.S., Nichols, N.N., Bischoff, K.M., Hughes, S.R., Cotta, M.A. 2007. Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis. FEMS Microbiolology Letters. 274(2):291-297.
Monensin inhibits growth of bacterial contaminants from fuel ethanol plants - (Abstract Only)
Bischoff, K.M., Liu, S., Dien, B.S. 2007. Monensin inhibits growth of bacterial contaminants from fuel ethanol plants [abstract]. American Society for Microbiology. Poster #0-010. p. 60.
Cloning and characterization of a recombinant family 5 endoglucanase from Bacillus licheniformis strain B-41361 - (Peer Reviewed Journal)
Bischoff, K.M., Liu, S., Hughes, S.R. 2007. Cloning and characterization of a recombinant family 5 endoglucanase from Bacillus licheniformis strain B-41361. Process Biochemistry. 42:1150-1154.
A novel Lactobacillus buchneri strain converts a concentrated mixture of C5 and C6 sugars into ethanol and other products - (Abstract Only)
Liu, S., Skinner-Nemec, K.A., Leathers, T.D. 2007. A novel Lactobacillus buchneri strain converts a concentrated mixture of C5 and C6 sugars into ethanol and other products [abstract]. Society of Industrial Microbiology Annual Meeting. p. 158.
High-Throughput Fully Automated Construction of a Multiplex Library of Mutagenized Open Reading Frames for an Insecticidal Peptide Using a Plasmid-Based Functional Proteomic Robotic Workcell with Improved Vacuum System - (Peer Reviewed Journal)
Hughes, S.R., Dowd, P.F., Hector, R.E., Riedmuller, S.B., Bartolett, S., Mertens, J.A., Qureshi, N., Liu, S., Bischoff, K.M., Li, X., Jackson Jr, J.S., Sterner, D., Panavas, T., Cotta, M.A., Farrelly, P.J., Butt, T. 2007. High-throughput fully automated construction of a multiplex library of mutagenized open reading frames for an insecticidal peptide using a plasmid-based functional proteomic robotic workcell with improved vacuum system. Journal of Laboratory Automation. 12(4):202-212.
Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose Expressing "Stealth" Insecticidal Peptides. - (Abstract Only)
Hughes, S.R., Li, X., Hector, R.E., Bischoff, K.M., Cotta, M.A., Mertens, J.A., Dowd, P.F., Johnson, E.T. 2007. Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and characterization of yeast strains optimized for growth on xylose expressing "stealth" insecticidal peptides [abstract]. American Chemical Society. p. 108.
Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Improved Growth on Xylose Expressing Xylanase A(Xyn A) and Celulase F(Cel F) from Anaerobic Fungi Orpinomyces - (Abstract Only)
Hughes, S.R., Mertens, J.A., Qureshi, N., Cotta, M.A., Bischoff, K.M., Liu, S., Li, X. 2007. Plasmid-based functional proteomic workcell evaluation and characterization of yeast strains with improved growth on xylose expressing Xylanase A(Xyn A) and Celulase F(Cel F) from anaerobic fungi Orpinomyces [abstract]. World Congress on Industrial Biotechnology and Bioprocessing. Seminar 2. p. 19.
Adaptation to an automated platform of algorithmic combinations of advantageous mutations in genes generated using amino acid scanning mutational strategy. - (Abstract Only)
Riedmuller, S., Hughes, S.R., Hector, R.E., Bischoff, K.M., Farrelly, P., Cotta, M.A., Li, X. 2007. Adaptation to an automated platform of algorithmic combinations of advantageous mutations in genes generated using amino acid scanning mutational strategy [abstract]. PEPTALK 2007. Cambridge Healthtech Institute. Seminar 43. p. 4.
Fully Automated Molecular Biology Routines on a Plasmid-Based Functional Proteomic Workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose and Engineered to Express an Insecticidal Peptide - (Abstract Only)
Hughes, S.R., Li, X., Liu, S., Bischoff, K.M., Mertens, J.A., Qureshi, N., Riedmuller, S., Bartolett, S., Farrelly, P., Panavas, T., Butt, T., Cotta, M.A. 2007. Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and characterization of yeast strains optimized for growth on xylose and engineered to express an insecticidal peptide [abstract]. PEPTALK 2007, Cambridge Healthtech Institute. Talk 83, p. 7.
Fully automated molecular biology: Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Optimized "Trojan Horse" Amino Acid Scanning Mutational Inserts. - (Abstract Only)
Hughes, S.R., Qureshi, N., Mertens, J.A., Cotta, M.A., Hector, R.E., Bischoff, K.M., Liu, S., Li, X-L., 2007. Fully automated molecular biology: Plasmid-based functional proteomic workcell evaluation and characterization of yeast strains with optimized "Trojan Horse" amino acid scanning mutational inserts [abstract]. PEPTALK 2007. Cambridge Healthtech Institute. Poster 509, p. 4.
Developing Gram-Positive Ethanologens - (Abstract Only)
Liu, S. 2006. Developing gram-positive ethanologens [abstract]. International Symposium of Biocatalysis and Bioenergy. p. 33.
Chromosomal Inactivation of the Als Gene in Lactobacillus Plantarum Strain Tf103 to Eliminate Undesired Fermentation Products - (Abstract Only)
Liu, S. 2006. Chromosomal inactivation of the als gene in Lactobacillus plantarum strain TF103 to eliminate undesired fermentation products [abstract]. Society of Industrial Microbiology. p. 166.
Purification and Characterization of a Family 5 Endoglucanase from a Moderately Thermophilic Strain of Bacillus Licheniformis - (Peer Reviewed Journal)
Bischoff, K.M., Rooney, A.P., Li, X., Liu, S., Hughes, S.R. 2006. Purification and characterization of a family 5 endoglucanase from a moderately thermophilic strain of Bacillus licheniformis. Biotechnology Letters. 28:1761-1765.
Genetically Engineered Lactic Acid Bacteria for the Production of Fuels and Chemicals - (Abstract Only)
Liu, S. 2006. Genetically engineered lactic acid bacteria for the production of fuels and chemicals [abstract]. 10th International Symposium on the Genetics of Industrial Microorganisms. Paper #015.
Isolation of Xylose-Utilizing Thermophilic Bacteria from Dairy Manure Compost - (Abstract Only)
Bischoff, K.M., Liu, S., Edrington, T.S., Hughes, S.R. 2006. Isolation of xylose-utilizing thermophilic bacteria from dairy manure compost [abstract]. Extremophiles 2006. p. 19. Poster P045.
Antimicrobial Susceptibility of Lactobacillus Species Isolated from Fuel-Ethanol Plants - (Abstract Only)
Bischoff, K.M., Skinner, K.A., Leathers, T.D. 2006. Antimicrobial susceptibility of lactobacillus species isolated from fuel-ethanol [abstract]. American Society for Microbiology. Poster No. O-038.
Functional Proteomic Plasmid-Based Integrated Workcell for High-Throughput Transformation of Bl21 De3 E. Coli for Expression in Vivo with Piromyces Strain Xylose Isomerase - (Abstract Only)
Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Li, X., Bischoff, K.M., Liu, S., Qureshi, N., Cotta, M.A., Skory, C.D., Gorsich, S.W., Farrelly, P.J. 2006. Functional proteomic plasmid-based integrated workcell for high-throughput transformation of BL21 DE3 E. coli for expression in vivo with piromyces strain xylose isomerase [abstract]. Midwest Laboratory Robotics Information Group. p. 2.
High-Throughput Screening of Cellulase F Mutants from Multiplexed Plasmid Sets Using An Automated Plate Assay on a Functional Proteomic Robotic Workcell - (Peer Reviewed Journal)
Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Li, X., Bischoff, K.M., Qureshi, N., Cotta, M.A., Farrelly, P.J. 2006. High-throughput screening of cellulase F mutants from multiplexed plasmid sets using an automated plate assay on a functional proteomic robotic workcell. Proteome Science. 4:10.
A Cold-Sensitive Listeria Monocytogenes Mutant Has a Transposon Insertion in a Gene Encoding a Putative Membrane Protein and Shows Altered (P)ppgpp Levels - (Peer Reviewed Journal)
Liu, S., Bayles, D.O., Mason, T.M., Wilkinson, B.J. 2006. A cold-sensitive Listeria monocytogenes mutant has a transposon insertion in a gene encoding a putative membrane protein and shows altered (p)ppGpp levels. Applied and Environmental Microbiology. 72(6):3955-3959.
Automated Strategy Using a Functional Proteomic Assay to Identify and Isolate Cellulase F Mutants with Improved Activity from Multiplexed Sets of Plasmid - (Abstract Only)
Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Qureshi, N., Farrelly, P., Cotta, M.A. 2006. Automated strategy using a functional proteomic assay to identify and isolate cellulase F mutants with improved activity from multiplexed sets of plasmid [abstract]. PepTalk 2006. p. 10.
Mass Transformation of Plasmid Libraries of Cdna Or Mutagenized Clone Sets into Yeast Or Bacteria Using a Functional Proteomic Robotic Workcell - (Abstract Only)
Hughes, S.R., Riedmuller, S., Li, X., Qureshi, N., Liu, S., Bischoff, K.M., Cotta, M.A., Farrelly, P. 2006. Mass transformation of plasmid libraries of cDNA or mutagenized clone sets into yeast or bacteria using a functional proteomic robotic workcell [abstract]. PepTalk 2006. p. 10.
Plasmid-Based Functional Proteomic Robotic Workcell Process for High-Throughput Screening of Multiplexed Libraries of Mutagenized Clones - (Abstract Only)
Hughes, S.R., Mertens, J.A., Li, X., Bischoff, K.M. 2005. Plasmid-based functional proteomic robotic workcell process for high-throughput screening of multiplexed libraries of mutagenized clones [abstract]. Laboratory Robotics Information Group. p. 1.
A Simple Method to Generate Chromosomal Mutations in Lactobacillus Plantarum Strain Tf103 to Eliminate Undesired Fermentation Products - (Peer Reviewed Journal)
Liu, S. 2006. A simple method to generate chromosomal mutations in Lactobacillus plantarum strain TF103 to eliminate undesired fermentation products. Applied Biochemistry and Biotechnology. 129-132:854-863.
Plasmid-Based Functional Proteomic Robotic Workcell Process for High-Throughput Screening of Multiplexed Libraries of Mutagenized Clones - (Abstract Only)
Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Qureshi, N., Bischoff, K.M., Jordan, D.B., Cotta, M.A., Farrelly, P. 2005. Plasmid-based functional proteomic robotic workcell process for high-throughput screening of multiplexed libraries of mutagenized clones [abstract]. Optimization high-throughput Cultures for Bioprocessing 2005. p. 3.
Functional Proteomic Workcell for High Volume Plasmid Preparations for Repeated in Vitro Protein Expression and High Throughput Screening to Identify Mutant Enyzmes for Use at Low Ph - (Abstract Only)
Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Jordan, D.B., Li, X., Qureshi, N., Cotta, M.A., Farrelly, P.J., Bischoff, K.M. 2005. Functional proteomic workcell for high volume plasmid preparations for repeated in vitro protein expression and high throughput screening to identify mutant enyzmes for use at low pH [abstract]. Optimization High-throughput Cultures for Bioprocessing 2005. 13:3.
Antimicrobial Susceptibility of Bacterial Contaminants from a Wet-Mill Ethanol Plant - (Abstract Only)
Bischoff, K.M., Skinner-Nemec, K., Leathers, T.D., Hughes, S.R. 2005. Antimicrobial susceptibility of bacterial contaminants from a wet-mill ethanol plant [abstract]. Society for Industrial Microbiology. Poster P42.
Characterization of Carboxymethylcellulase Activity from Geobacillus Stearothermophilus - (Abstract Only)
Bischoff, K.M., Li, X., Rooney, A.P., Liu, S., Hughes, S.R. 2005. Characterization of carboxymethylcellulase activity from Geobacillus stearothermophilus [abstract]. Society for Industrial Microbiology. Paper #P38.
Lactobacillus Brevis: a Potential Biocatalyst for Lignocellulosic Biomass to Ethanol - (Abstract Only)
Liu, S., Dien, B.S., Cotta, M.A., Bischoff, K.M., Hughes, S.R. 2005. Lactobacillus brevis: a potential biocatalyst for lignocellulosic biomass to ethanol [abstract]. Society of Industrial Microbiology. Paper #P05.
Development of a Liquid Handler Component for a Plasmid-Based Functional Proteomic Robotic Workcell - (Peer Reviewed Journal)
Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Bischoff, K.M., Cotta, M.A., Farrelly, P. 2005. Development of a liquid handler component for a plasmid-based functional proteomic robotic workcell. Journal of the Association for Laboratory Automation. 10(5):287-300.
Metabolic Engineering of a Lactobacillus Plantarum Double Ldh Knockout Strain for Enhanced Ethanol Production - (Peer Reviewed Journal)
Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2006. Metabolic engineering of a Lactobacillus plantarum double LDH knockout strain for enhanced ethanol production. Journal of Industrial Microbiology and Biotechnology. 33(1):1-7.
Ethanol Fermentation by Overexpressing a Gram-Positive Pdc Gene in Lactobacillus Plantarum Strain Tf103 - (Abstract Only)
Liu, S., Nichols, N.N., Dien, B.S., Cotta, M.A. 2005. Ethanol fermentation by overexpressing a Gram-positive PDC gene in Lactobacillus plantarum strain TF103 [abstract]. Biotechnology for Fuels and Chemicals Symposium Proceedings, May 1-4, 2005, Denver, Colorado. p. 158.
Metabolic Engineering of Lactobacillus Brevis for Ethanol Production - (Abstract Only)
Liu, S., Dien, B.S. 2005. Metabolic engineering of Lactobacillus brevis for ethanol production [abstract]. The World Congress on Industrial Biotechnology and Bioprocessing. p. 23.
Development of a Liquid Handler Component for a Functional Plasmid-Based Proteomic Workcell That Generates Multiplex Samples Expresses in Yeast - (Abstract Only)
Hughes, S.R., Riedmuller, S.B., Bischoff, K.M., Mertens, J.A., Li, X., Cotta, M.A., Farrelly, P.J. 2005. Development of a liquid handler component for a functional plasmid-based proteomic workcell that generates multiplex samples expresses in yeast [abstract]. Association for Laboratory Automation, LabAutomation 2005. Poster WP128.
Modified Liquid Handler Used to Produce High Throughput High Quality Plasmids for Integration into Plasmid-Based Functional Proteomics Workcell - (Abstract Only)
Hughes, S.R., Li, X., Cotta, M.A., Mertens, J.A., Bischoff, K.M., Riedmuller, S., Farrelly, P., Patel, M., Brown, L., Carter, L. 2005. Modified liquid handler used to produce high throughput high quality plasmids for integration into plasmid-based functional proteomics workcell [abstract]. CHI's Annual PEP Talk Meeting, January 10-14, 2005, San Diego, California. Poster 151.
A Plasmid-Based Functional Proteomic Workcell Assay Designed to Screen Orpinomyces Cellulase F Nny Mutagenized Libraries in High Throughtput - (Abstract Only)
Hughes, S.R., Li, X., Cotta, M.A., Bischoff, K.M., Riedmuller, S., Farrelly, P. 2004. A plasmid-based functional proteomic workcell assay designed to screen Orpinomyces cellulase F NNY mutagenized libraries in high throughtput [abstract]. 36th Great Lakes Regional American Chemical Society Symposium, October 17-20, 2004, Peoria, Illinois. Poster 361.
Insights into Growth of Listeria Monocytogenes at Refrigeration Temperatures Through Studies of Tn917-Induced Cold-Sensitive Mutants - (Abstract Only)
Zhu, K., Liu, F., Liu, S., Wilkinson, B.J. 2003. Insights into growth of Listeria monocytogenes at refrigeration temperatures through studies of Tn917-induced cold-sensitive mutants [abstract]. Great Lake Regional Center of Excellence, First Annual Conference, Northwestern University, December 12-14, 2003, Chicago, Illinois. p. 25.
Last Modified: 8/21/2014
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