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Research Project:
INDUSTRIALLY ROBUST ENZYMES AND MICROORGANISMS FOR PRODUCTION OF SUGARS AND ETHANOL FROM AGRICULTURAL BIOMASS
Location: National Center for Agricultural Utilization Research
Publications
(Clicking on the reprint icon
will take you to the publication reprint.)
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Catalytic properties of two Rhizopus oryzae 99-880 glucoamylase enzymes without starch binding domains expressed in Pichia pastoris
- (Peer Reviewed Journal)
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| Mertens, J.A., Braker, J.D., Jordan, D.B. 2010. Catalytic properties of two Rhizopus oryzae 99-880 glucoamylase enzymes without starch binding domains expressed in Pichia pastoris. Applied Biochemistry and Biotechnology. 162(8):2197-2213. |
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Fermentation of Bioenergy Crops Into Ethanol Using Biological Abatement for Removal of Inhibitors
- (Peer Reviewed Journal)
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| Nichols, N.N., Dien, B.S., Cotta, M.A. 2010. Fermentation of Bioenergy Crops Into Ethanol Using Biological Abatement for Removal of Inhibitors. Bioresource Technology. 101(19):7545-7550. |
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Use of Coniochaeta ligniaria to Detoxify Fermentation Inhibitors Present in Cellulosic Sugar Streams
- (Book / Chapter)
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| Nichols, N.N., Dien, B.S., Lopez, M.J., Moreno, J. 2010. Use of Coniochaeta ligniaria to Detoxify Fermentation Inhibitors Present in Cellulosic Sugar Streams. In: Hou, C.T., Shaw, J.-F., editors. Biocatalysis and Molecular Engineering. New York: John Wiley and Sons. p. 253-263. |
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Beta-D-xylosidase from Selenomonas ruminantium: Role of Glutamate 186 in Catalysis Revealed by Site-Directed Mutagenesis, Alternate Substrates, and Active-site Inhibitor
- (Peer Reviewed Journal)
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| Jordan, D.B., Braker, J.D. 2010. Beta-D-xylosidase from Selenomonas ruminantium: Role of Glutamate 186 in Catalysis Revealed by Site-directed Mutagenesis, Alternate Substrates, and Active-site Inhibitor. Applied Biochemistry and Biotechnology. 161(1-8):395-410. |
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Inhibition of cellulases by phenols
- (Peer Reviewed Journal)
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| Ximenes, E., Kim, Y., Mosier, N., Dien, B.S., Ladisch, M. 2010. Inhibition of cellulases by phenols. Enzyme and Microbial Technology. 46(3-4):170-176. |
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Increasing Biomass Conversion Efficiencies to Ethanol by Engineering Energy Crops
- (Proceedings)
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| Dien, B.S., Sarath, G., Pedersen, J.F., Funnell-Harris, D.L., Sattler, S.E., Cotta, M.A. 2009. Increasing Biomass Conversion Efficiencies to Ethanol by Engineering Energy Crops. Proceedings of the United States-Japan Natural Resources Food and Agriculture Panel. p. 37-40. |
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Beta-D-xylosidase from Selenomonas ruminantium: Role of Glutamate 186 in Catalysis Revealed by Site-directed Mutagenesis, Alternate Substrates, and Inhibitor
- (Abstract Only)
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| (31-Jul-09) |
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Identification, Characterization, and Evolution of a Large Polygalacturonase Gene Family from Rhizopus oryzae
- (Abstract Only)
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| (31-Jul-09) |
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Anaerobic conversion of lignocellulosic corn fiber to butyric acid, a substrate for microbial butanol production
- (Abstract Only)
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| (22-Jul-09) |
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Ion Exchange Membrane Influence on Ohmic Resistance
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| (12-Jun-09) |
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Efficiencies of Bio-electrocatalytic Production of Hydrogen from Lactate Using Shewanella oneidensis MR-1
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| (12-Jun-09) |
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Use of Coniochaeta ligniaria to detoxify fermentation inhibitors present in cellulosic sugar streams
- (Abstract Only)
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| Nichols, N.N., Dien, B.S., Cotta, M.A. 2009. Use of Coniochaeta ligniaria to detoxify fermentation inhibitors present in cellulosic sugar streams [abstract]. American Society for Microbiology. Paper No. O-055. |
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Conversion of switchgrass to sugars and ethanol using dilute ammonium hydroxide pretreatment
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| (06-May-09) |
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Beta-D-xylosidase from Selenomonas ruminantium: role of glutamate 186 in catalysis revealed by site-directed mutagenesis, alternate substrates, and inhibitors
- (Abstract Only)
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| (06-May-09) |
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Improved Sugar Conversion and Ethanol Yield for Forage Sorghum (Sorghum bicolor L. Moench) Lines with Reduced Lignin Contents
- (Peer Reviewed Journal)
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| Dien, B.S., Sarath, G., Pedersen, J.F., Sattler, S.E., Chen, H., Funnell-Harris, D.L., Nichols, N.N., Cotta, M.A. 2009. Improved Sugar Conversion and Ethanol Yield for Forage Sorghum (Sorghum bicolor L. Moench) Lines with Reduced Lignin Contents. BioEnergy Research. 2(3):153-164. |
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Conversion of lower lignin mutants of Sorghum bicolor (L.) to ethanol
- (Abstract Only)
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| (13-Mar-09) |
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Development of an Ethanol Yield Procedure for Dry-grind Corn Processing
- (Peer Reviewed Journal)
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| Lemuz, C.R., Dien, B.S., Singh, V., Mckinney, J., Tumbleson, M.E., Rausch, K.D. 2009. Development of an Ethanol Yield Procedure for Dry-grind Corn Processing. Cereal Chemistry. 86(3):355-360. |
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Measurement of Hydrocarbon Transport in Bacteria
- (Book / Chapter)
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| Ditty, J.L., Nichols, N.N., Parales, R.E. 2010. Measurement of Hydrocarbon Transport in Bacteria. In: Timmis, K.N., editor. Handbook of Hydrocarbon and Lipid Microbiology. Berlin Heidelberg: Springer-Verlag. p. 4213-4222. |
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Mass Balances and Analytical Methods for Biomass Pretreatment Experiments
- (Book / Chapter)
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| Dien, B.S. 2010. Mass Balances and Analytical Methods for Biomass Pretreatment Experiments. In: Vertes, A. A., Qureshi, N., Blaschek, H. P., Yukawa, H., editors. Biomass to Biofuels. Strategies for Global Industries. United Kingdom: Wiley and Sons. p. 213-231. |
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Novel Family of Carbohydrate Esterases, Based on Identification of the Hypocrea jecorina Acetyl Esterase Gene
- (Peer Reviewed Journal)
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| Li, X., Skory, C.D., Cotta, M.A., Puchart, V., Biely, P. 2008. Novel family of carbohydrate esterases, based on identification of the Hypocrea jecorina Acetyl Esterase Gene. Applied and Environmental Microbiology. 74(24):7482-7489. |
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Beta-D-xylosidase from Selenomonas ruminantium: thermodynamics of enzyme-catalyzed and noncatalyzed reactions
- (Peer Reviewed Journal)
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| Jordan, D.B., Braker, J.D. 2009. Beta-D-xylosidase from Selenomonas ruminantium: Thermodynamics of Enzyme-catalyzed and Noncatalyzed Reactions. Applied Biochemistry and Biotechnology. 155(1-3):330-346. |
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Identification, Biochemical Characterization, and Evolution of the Rhizopus oryzae 99-880 Polygalacturonase Gene Family
- (Peer Reviewed Journal)
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| Mertens, J.A., Burdick, R.C., Rooney, A.P. 2008. Identification, Biochemical Characterization, and Evolution of the Rhizopus oryzae 99-880 Polygalacturonase Gene Family. Fungal Genetics and Biology. 45(12):1616-1624. |
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Simultaneous Saccharification and Fermentation and Partial Saccharification and Co-fermentation of Lignocellulosic Biomass for Ethanol Production
- (Book / Chapter)
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| (26-Sep-08) |
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Aminoalcohols as Probes of the Two-subsite Active Site of Beta-D-xylosidase from Selenomonas ruminantium
- (Peer Reviewed Journal)
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| Jordan, D.B., Mertens, J.A., Braker, J.D. 2009. Aminoalcohols as Probes of the Two-subsite Active Site of Beta-D-xylosidase from Selenomonas ruminantium. Biochimica et Biophysica Acta. 1794(1):144-158. |
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Challenges of converting agricultural abundance to biofuels: Agricultural Research Service-United States Department of Agriculture Research
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| (28-Aug-08) |
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Microbial Fuel Cell Performance with a Pressurized Cathode Chamber
- (Peer Reviewed Journal)
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| Fornero, J.J., Rosenbaum, M., Cotta, M.A., Angenent, L.T. 2008. Microbial Fuel Cell Performance with a Pressurized Cathode Chamber. Environmental Science and Technology. 42(22):8578-8584. |
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Opportunities for increasing biomass conversion efficiencies to ethanol by engineering energy crops
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| (17-Aug-08) |
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Isolation and characterization of 17 different genes encoding putative endopolygalacturonase genes from Rhizopus oryzae
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| Mertens, J.A., Burdick, R.C. 2008. Isolation and characterization of 17 different genes encoding putative endopolygalacturonase genes from Rhizopus oryzae [abstract]. Society of Industrial Microbiology. p. 116. |
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Beta-D-xylosidase from Selenomonas ruminantium: thermodynamics of enzyme-catalyzed and noncatalyzed reactions
- (Abstract Only)
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| (18-Jul-08) |
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Thin Stillage Fractionation Using Ultrafiltration: Resistance in Series Model
- (Peer Reviewed Journal)
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| Arora, A., Dien, B.S., Belyea, R.L., Wang, P., Singh, V., Tumbleson, M.E., Rausch, K.D. 2009. Thin Stillage Fractionation Using Ultrafiltration: Resistance in Series Model. Bioprocess and Biosystems Engineering. 32(2):225-233. |
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Evaluating low lignin mutants of forage sorghum for increased conversion efficiency to sugars and ethanol
- (Abstract Only)
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| Dien, B.S., Sarath, G., Pedersen, J.F., Funnell-Harris, D.L., Sattler, S.E., Nichols, N.N. 2008. Evaluating low lignin mutants of forage sorghum for increased conversion efficiency to sugars and ethanol. Biotechnology for Fuels and Chemicals. p. 267. |
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Beta-D-xylosidase from Selenomonas ruminantium: thermodynamics of enzyme-catalyzed and noncatalyzed reactions
- (Abstract Only)
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| (07-May-08) |
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Beta-D-xylosidase from Selenomonas ruminantium: catalyzed reactions with natural substrates
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| (07-May-08) |
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Energy crops for ethanol: a processing perspective
- (Abstract Only)
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| Dien, B.S. 2008. Energy crops for ethanol: a processing perspective. International Crop Science. Paper No. CS4-S4-06. |
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Energy crops for ethanol: a processing perspective
- (Proceedings)
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| Dien, B.S., Sarath, G., Pedersen, J.F., Vogel, K.P., Jung, H.G., Sattler, S.E., Casler, M.D., Mitchell, R., Cotta, M.A. 2008. Energy crops for ethanol: a processing perspective. In: Proceedings of the 5th International Crops Science Congress, April 13-18, 2008, Jeju Island, Korea. p. 1-5. |
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Identification and transcriptional profiling of Pseudomonas putida genes involved in furoic acid metabolism
- (Peer Reviewed Journal)
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| Nichols, N.N., Mertens, J.A. 2008. Identification and transcriptional profiling of Pseudomonas putida genes involved in furoic acid metabolism. FEMS Microbiology Letters. 284:52-57. |
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Structure of the two-subsite beta-D-xylosidase from Selenomonas ruminantium in complex with 1,3-bis[tris(hydroxymethyl)methylamino]propane
- (Peer Reviewed Journal)
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| Brunzelle, J.S., Jordan, D.B., McCaslin, D.R., Olczak, A., Wawrzak, Z. 2008. Structure of the two-subsite beta-D-xylosidase from Selenomonas ruminantium in complex with 1,3-bis[tris(hydroxymethyl)methylamino] propane. Archives Of Biochemistry and Biophysics. 474(1):157-166. |
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Crystallization and Preliminary X-ray Diffraction Analysis of the Glucuronoyl Esterase Catalytic Domain from Hypocrea jecorina
- (Peer Reviewed Journal)
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| Wood, S.J., Li, X., Cotta, M.A., Biely, P., Duke, N.E., Schiffer, M., Pokkuluri, P.R. 2008. Crystallization and Preliminary X-ray Diffraction Analysis of the Glucuronoyl Esterase Catalytic Domain from Hypocrea jecorina. Acta Crystallographica Section F. 64(4):255-257. |
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Fungal metabolism of fermentation inhibitors present in corn stover dilute acid hydrolysate
- (Peer Reviewed Journal)
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| Nichols, N.N., Sharma, L.N., Mowery, R.A., Chambliss, C.K., Van Walsum, G.P., Dien, B.S., Iten, L.B. 2008. Fungal metabolism of fermentation inhibitors present in corn stover dilute acid hydrolysate. Enzyme and Microbial Technology. 42(7):624-630. |
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PRODUCTION OF ETHANOL FROM CORN AND SUGARCANE
- (Book / Chapter)
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| Nichols, N.N., Dien, B.S., Monceaux, D.A., Bothast, R.J. 2008. Production of ethanol from corn and sugarcane. In: Wall, J., Harwood, C.S., Demain, A.L., editors. Bioenergy. Chapter 1. Washington, DC: ASM Press. p. 3-15. |
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PRODUCTION OF ETHANOL FROM GRAIN
- (Book / Chapter)
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| Nichols, N.N., Bothast, R.J. 2008. Production of ethanol from grain. In: Vermerris, W., editor. Genetic Improvement of Bioenergy Crops. New York, NY: Springer. p. 75-88. |
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Process review of lignocellulose biochemical conversion to fuel ethanol
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| Dien, B.S. 2007. Process review of lignocellulose biochemical conversion to fuel ethanol. Proceedings of U.S. Frontiers of Engineering. p. 65-74. |
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Developing herbaceous energy crops as feedstocks for biofuel production
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| Dien, B.S., Jung, H.G., Vogel, K.P., Casler, M.D., Lamb, J.F., Iten, L.B., Mitchell, R., Sarath, G. 2007. Developing herbaceous energy crops as feedstocks for biofuel production. In: Proceedings of the UJNR Food and Agricultural Panel, October 21-25, 2007, Tsukuba, Japan. p. 137-140. |
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BETA-D-XYLOSIDASE FROM SELENOMONAS RUMINANTIUM: CATALYZED REACTIONS WITH NATURAL AND ARTIFICIAL SUBSTRATES
- (Peer Reviewed Journal)
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| Jordan, D.B. 2008. Beta-D-xylosidase from Selenomonas ruminantium: catalyzed reactions with natural and artificial substrates. Applied Biochemistry and Biotechnology. 146:137-149. |
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Enzyme characterization for hydrolysis of AFEX and liquid hot-water pretreated distillers' grains and their conversion to ethanol
- (Peer Reviewed Journal)
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| Dien, B.S., Ximenes, E.A., O'Bryan, P.J., Moniruzzaman, M., Li, X., Balan, V., Dale, B., Cotta, M.A. 2008. Enzyme characterization for hydrolysis of AFEX and liquid hot-water pretreated distillers' grains and their conversion to ethanol. Bioresource Technology. 99(12):5216-5225. |
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Composition of corn dry-grind ethanol by-products: DDGS, wet cake, and thin stillage
- (Peer Reviewed Journal)
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| Kim, Y., Mosier, N.S., Hendrickson, R., Ezeji, T., Blaschek, H., Dien, B.S., Cotta, M.A., Dale, B., Ladisch, M.R. 2008. Composition of corn dry-grind ethanol by-products: DDGS, wet cake, and thin stillage. Bioresource Technology. 99(12):5165-5176. |
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STRUCTURE-FUNCTION RELATIONSHIPS OF A CATALYTICALLY-EFFICIENT BETA-D-XYLOSIDASE
- (Abstract Only)
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| (03-Aug-07) |
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THE CESA (CE3B) CARBOXY-TERMINAL DOMAIN OF RUMINOCOCCUS FLAVEFACIENS 17 HAS GLUCURONOYL ESTERASE ACTIVITY
- (Abstract Only)
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| (03-Aug-07) |
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Identification of Genes Encoding Microbial Glucuronoyl Esterases
- (Peer Reviewed Journal)
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| Li, X., Spanikova, S., De Vries, R.P., Biely, P. 2007. Identification of Genes Encoding Microbial Glucuronoyl Esterases. The Federation of European Biochemical Societies. 581(21):4029-4035. |
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VARIATION IN RELATIVE SUBSTRATE SPECIFICTY OF BIFUNCTIONAL BETA-D-XYLOSIDASE/ALPHA-L-ARABINOFURANOSIDASE BY SINGLE-SITE MUTATIONS: ROLES OF SUBSTRATE DISTORTION AND RECOGNITION
- (Peer Reviewed Journal)
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| Jordan, D.B., Li, X. 2007. Variation in relative substrate specificity of bifunctional beta-D-xylosidase/alpha-L-arabinofuranosidase by single-site mutations: roles of substrate distortion and recognition. Biochimica et Biophysica Acta. 1774(9):1192-1198. |
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FIBER SEPARATED FROM DISTILLERS DRIED GRAINS WITH SOLUBLES AS A FEEDSTOCK FOR ETHANOL PRODUCTION TO INCREASE OUTPUT FROM DRY GRIND CORN PLANTS
- (Peer Reviewed Journal)
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| Srinivasan, R., Dien, B.S., Rausch, K.D., Tumbleson, M.E., Singh, V. 2007. Fiber Separated from Distillers Dried Grains with Solubles as a Feedstock for Ethanol Production. Cereal Chemistry. 84(6):563-566. |
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A PRIMER FOR LIGNOCELLULOSE BIOCHEMICAL CONVERSION TO FUEL ETHANOL
- (Proceedings)
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| Dien, B.S. 2007. A primer for lignocellulose biochemical conversion to fuel ethanol. Proceedings of the Fifth International Starch Technology Conference. p. 5.1-5.9. |
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INHIBITION OF THE TWO-SUBSITE BETA-D-XYLOSIDASE FROM SELENOMONAS RUMINANTIUM BY SUGARS: COMPETITIVE, NONCOMPETITIVE, DOUBLE BINDING, AND SLOW BINDING MODES
- (Peer Reviewed Journal)
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| Jordan, D.B., Braker, J.D. 2007. Inhibition of the two-subsite beta-d-xylosidase from Selenomonas ruminantium by sugars: competitive, noncompetitive, double binding, and slow binding modes. Archives of Biochemistry and Biophysics. 465(1):231-246. |
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A NOVEL PRETREATMENT (DIETHYL OXALATE) RELEASING FERMENTABLE SUGARS AND USEFUL FIBERS FROM LIGNOCELLULOSE
- (Abstract Only)
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| (02-May-07) |
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BETA-D-XYLOSIDASE FROM SELENOMONAS RUMINANTIUM: THERMODYNAMICS OF ENZYME-CATALYZED AND NONCATALYZED REACTIONS
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| Jordan, D.B. 2007. Beta-D-xylosidase from Selenomonas ruminantium: thermodynamics of enzyme-catalyzed and noncatalyzed reactions [abstract]. Biotechnology for Fuels and Chemicals. p. 105. |
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BETA-D-XYLOSIDASE FROM SELENOMONAS RUMINANTIUM: CATALYZED REACTIONS WITH NATURAL AND ARTIFICIAL SUBSTRATES
- (Abstract Only)
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| Jordan, D.B. 2007. Beta-D-xylosidase from Selenomonas ruminantium: catalyzed reactions with natural and artificial substrates [abstract]. Biotechnology for Fuels and Chemicals. p. 106. |
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REMOVAL OF INHIBITORS FROM BIOMASS SUGARS USING A BIOLOGICAL PROCESS
- (Abstract Only)
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| (02-May-07) |
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EVALUATING HERBACEOUS PERENNIALS AS ENERGY CROPS FOR PRODUCTION OF FUEL ETHANOL
- (Abstract Only)
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| (02-May-07) |
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DEVELOPING HERBACEOUS ENERGY CROPS AS FEEDSTOCKS FOR BIOETHANOL PRODUCTION
- (Abstract Only)
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| Dien, B.S., Cotta, M.A., Jung, H.G., Vogel, K.P. 2007. Developing herbaceous energy crops as feedstocks for bioethanol production [abstract]. American Chemical Society. Paper No. Agro-148. |
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BIOLOGICAL ABATEMENT FOR REMOVAL OF INHIBITORS FROM BIOMASS SUGARS
- (Abstract Only)
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| (29-Mar-07) |
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STRUCTURE-FUNCTION RELATIONSHIPS OF A CATALYTICALLY EFFICIENT BETA-D-XYLOSIDASE
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| Jordan, D.B. 2007. Structure-function relationships of a catalytically efficient beta-D-xylosidase [abstract]. American Chemical Society. Paper No. 175. |
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ISOLATION AND CHARACTERIZATION OF TWO GENES THAT ENCODE ACTIVE GLUCOAMYLASE WITHOUT A STARCH BINDING DOMAIN FROM A TYPE II RHIZOPUS ORYZAE
- (Peer Reviewed Journal)
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| Mertens, J.A., Skory, C.D. 2007. Isolation and characterization of two genes that encode active glucoamylase without a starch binding domain from Rhizopus oryzae. Current Microbiology. 54:462-466. |
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PRODUCTION OF HIGHLY ACTIVE LIGNOCELLULOSE-DEGRADING ENZYMES OF ANAEROBIC FUNGI BY INDUSTRIALLY RELEVANT FUNGI
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| Li, X., Skory, C.D., Ximenes, E.A., Jordan, D.B., Dien, B.S., Hughes, S.R., Cotta, M.A. 2007. Production of highly active lignocellulose-degrading enzymes of anaerobic fungi by industrially relevant fungi [abstract]. Physiology to Genomics to Fuels. |
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A primer for lignocellulose biochemical conversion to fuel ethanol
- (Book / Chapter)
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| Dien, B.S., Bothast, R.J. 2009. A primer for lignocellulose biochemical conversion to fuel ethanol. In: Ingledew,W.M., Austin, G.D., Kluhspies, C., Kelsall, D.R., editors. The Alcohol Textbook. Nottingham, U.K.: Nottingham University Press. p. 73-94. |
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PROTEIN EXPRESSION AND SECRETION BY TRICHODERMA REESEI UNDER LOW ENDOGENOUS PROTEIN BACKGROUND
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| (12-Jan-07) |
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FORAGE ENERGY CROPS AS FEEDSTOCKS FOR PRODUCTION OF FUEL ETHANOL
- (Abstract Only)
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| Cotta, M.A., Dien, B.S., Iten, L.B., Jung, H.G., Lamb, J.F., Vogel, K.P., Mitchell, R., Sarath, G., Casler, M.D., Weimer, P.J. 2006. Forage energy crops as feedstocks for production of fuel ethanol [abstract]. Biocatalysis and Bioenergy. p. 21. |
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EXPRESSION OF AN AT-RICH XYLANASE GENE FROM THE ANAEROBIC FUNGUS ORPINOMYCES SP. STRAIN PC-2 IN AND SECRETION OF THE HETEROLOGOUS ENZYME BY HYPOCREA JECORINA
- (Peer Reviewed Journal)
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| Li, X., Skory, C.D., Ximenes, E.A., Jordan, D.B., Dien, B.S., Hughes, S.R., Cotta, M.A. 2007. Expression of an AT-rich xylanase gene from the anaerobic fungus Orpinomyces sp. strain PC-2 in and secretion of the heterologous enzyme by Hypocrea jecorina. Applied Microbiology and Biotechnology. 74:1264-1275. |
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FUNGICIDAL CARBOXAMIDES
- (Other)
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| Basarab, G.S., Jordan, D.B., Lessen, T.A., Hansen, S.L. 2006. Fungicidal carboxamides. U.S. Patent 7,135,585. |
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BETA-D-XYLOSIDASE FROM SELENOMONAS RUMINANTIUM OF GLYCOSIDE HYDROLASE FAMILY 43
- (Peer Reviewed Journal)
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| Jordan, D.B., Li, X., Dunlap, C.A., Whitehead, T.R., Cotta, M.A. 2007. Beta-D-xylosidase from Selenomonas ruminantium of glycoside hydrolase family 43. Applied Biochemistry and Biotechnology. 136-140:93-104. |
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HEMICELLULASES FOR MEDIATING BIOMASS SACCHARIFICATION
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| (13-Oct-06) |
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HEMICELLULASES FOR MEDIATING BIOMASS SACCHARIFICATION
- (Proceedings)
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| Jordan, D.B., Dien, B.S., Li, X., Cotta, M.A. 2006. Hemicellulases for mediating biomass saccharification. In: Proceedings of Renewable Energy 2006, October 9-13, 2006, Makuhari Messe, Chiba, Japan. p. 1036-1041. |
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REVIEW OF CURRENT RESEARCH DIRECTIONS FOR PRODUCING BIOLOGICAL ETHANOL AT THE NATIONAL CENTER FOR AGRICULTURAL UTILIZATION RESEARCH
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| Dien, B.S., Saha, B.C. 2006. Review of current research directions for producing biological ethanol at the National Center for Agricultural Utilization Research [abstract]. Advancing Renewable Energy. p. 27. |
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BETA-D-XYLOSIDASE FROM SELENOMONAS RUMINANTIUM OF GLYCOSIDE HYDROLASE FAMILY 43
- (Abstract Only)
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| Jordan, D.B., Li, X., Dunlap, C.A., Whitehead, T.R., Cotta, M.A. 2006. Beta-D-xylosidase from Selenomonas ruminantium of glycoside hydrolase family 43 [abstract]. Gordon Research Conference. p. 3. |
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ISOLATION AND CHARACTERIZATION OF A SECOND GLUCOAMYLASE WITHOUT A STARCH BINDING DOMAIN FROM RHIZOPUS ORYZAE
- (Peer Reviewed Journal)
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| Mertens, J.A., Skory, C.D. 2007. Isolation and characterization of a second glucoamylase gene without a starch binding domain from Rhizopus oryzae. Enzyme and Microbial Technology. 40:874-880. |
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CULTURE CONTAINING BIOMASS ACID HYDROLYSATE AND CONIOCHAETA LIGNIARIA FUNGUS
- (Patent Application)
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| Nichols, N.N., Lopez, M.J., Dien, B.S., Bothast, R.J. 2006. Culture containing biomass acid hydrolysate and Coniochaeta ligniaria fungus. U.S. Patent 7,067,303. |
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LIGNOCELLULOSE-DEGRADING ENZYMES PRODUCED BY THE ASCOMYCETE CONIOCHAETA LIGNIARIA AND RELATED SPECIES: APPLICATION FOR A LIGNOCELLULOSIC SUBSTRATE TREATMENT
- (Peer Reviewed Journal)
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| Lopez, M.J., Vargas-Garcia, M., Suarez-Estrella, F., Nichols, N.N., Dien, B.S., Moreno, J. 2007. Lignocellulose-degrading enzymes produced by the ascomycete Coniochaeta ligniaria and related species: application for a lignocellulosic substrate treatment. Enzyme and Microbial Technology. 40:794-800. |
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PRETREATMENT AND HYDROLYSIS OF DISTILLER'S GRAINS TO FERMENTABLE SUGARS: AN INTEGRATED APPROACH BY THE MIDWEST CONSORTIUM FOR SUSTAINABLE BIOBASED PRODUCTS AND ENERGY
- (Abstract Only)
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| Cotta, M.A., Dien, B.S., Li, X., Ladisch, M., Mosier, N., Tyner, W., Woodson, R., Blaschek, H., Dale, B., Shanks, B., Verkade, J. 2006. Pretreatment and hydrolysis of distiller's grains to fermentable sugars: an integrated approach by the Midwest Consortium for sustainable biobased products and energy [abstract]. Corn Utilization. Paper No. 24. |
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ISOLATION OF EXTREMELY AT-RICH GENOMIC DNA AND ANALYSIS OF GENES ENCODING CARBOHYDRATE-DEGRADING ENZYMES FROM ORPINOMYCES SP. STRAIN PC-2
- (Peer Reviewed Journal)
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| Chen, H., Hopper, S.L., Li, X., Ljungdahl, L.G., Cerniglia, C.E. 2006. Isolation of extremely AT-rich genomic DNA and analysis of genes encoding carbohydrate-degrading enzymes from Orpinomyces sp. strain PC-2. Current Microbiology. 53:396-400. |
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HIGH LEVEL EXPRESSION AND CHARACTERIZATION OF THE CYCLOPHILIN B GENE FROM THE ANAEROBIC FUNGUS ORPINOMYCES SP. STRAIN PC-2
- (Peer Reviewed Journal)
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| Chen, H., Li, X., Xu, H., Ljungdahl, L.G., Cerniglia, C.E. 2006. High level expression and characterization of the cyclophilin B gene from the anaerobic fungus Orpinomyces sp. strain PC-2. Protein and Peptide Letters. 13:727-732. |
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IDENTIFICATION AND TRANSCRIPTIONAL PROFILING OF FUROIC ACID METABOLISM GENES IN PSEUDOMONAS PUTIDA
- (Abstract Only)
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| Nichols, N.N., Mertens, J.A., Dien, B.S. 2006. Identification and transcriptional profiling of furoic acid metabolism genes in Pseudomonas putida [abstract]. American Society for Microbiology. Paper No. Q-449. |
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AN OVERVIEW OF RECENT ADVANCEMENTS IN LIGNOCELLULOSE TO ETHANOL CONVERSION TECHNOLOGY
- (Abstract Only)
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| Dien, B.S., Nichols, N.N., Li, X., Cotta, M.A. 2006. An overview of recent advancements in lignocellulose to ethanol conversion technology [abstract]. International Conference on Bioenergy. p. 8. |
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BETA-D-XYLOSIDASE FROM SELENOMONAS RUMINANTIUM OF GLYCOSIDE HYDROLASE FAMILY 43
- (Abstract Only)
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| Jordan, D.B., Li, X., Dunlap, C.A., Whitehead, T.R., Cotta, M.A. 2006. Beta-D-xylosidase from Selenomonas ruminantium of glycoside hydrolase family 43 [abstract]. Biotechnology for Fuels and Chemicals. p.93 |
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RESEARCH CHALLENGES AND OPPORTUNITIES FOR CELLULOSE CONVERSION TECHNOLOGY IN A DRY MILL PATHWAY
- (Abstract Only)
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| Ladisch, M., Tyner, W., Mosier, N., Cotta, M.A., Dien, B.S., Blaschek, H., Dale, B., Shanks, B., Petersen, G. 2006. Research challenges and opportunities for cellulose conversion technology in a dry mill pathway [abstract]. Biotechnology for Fuels and Chemicals. p. 48. |
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PLASMIDS FOR EXPRESSION OF HETEROLOGOUS PROTEINS IN RHIZOPUS ORYZAE
- (Peer Reviewed Journal)
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| Mertens, J.A., Skory, C.D., Ibrahim, A.S. 2006. Plasmids for expression of heterologous proteins in Rhizopus oryzae. Archives of Microbiology. 186:41-50. |
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CODON OPTIMIZATION OF AN A+T RICH XYLANASE GENE FROM THE ANAEROBIC FUNGUS ORPINOMYCES PC-2 FOR EXPRESSION BY TRICHODERMA REESEI
- (Abstract Only)
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| Li, X., Jordan, D.B., Ximenes, E.A., Cotta, M.A., Dien, B.S., Hughes, S.R. 2006. Codon optimization of an A+T rich xylanase gene from the anaerobic fungus Orpinomyces PC-2 for expression by Trichoderma reesei [abstract]. In: Proceedings of the 9th International Workshop on Trichoderma and Gliocladium, April 6-8, 2006, Vienna, Austria. Paper No. T3. |
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DEVELOPMENT OF FORAGE CROPS AS FEEDSTOCKS FOR PRODUCTION OF FUEL ETHANOL
- (Abstract Only)
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| Cotta, M.A., Dien, B.S., Jung, H.G., Vogel, K.P., Casler, M.D., Lamb, J.F., Weimer, P.J., Iten, L.B., Mitchell, R., Sarath, G. 2006. Development of forage crops as feedstocks for production of fuel ethanol [abstract]. International Conference on Bioenergy. p. I-5. |
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CHEMICAL COMPOSITION AND RESPONSE TO DILUTE-ACID PRETREATMENT AND ENZYMATIC SACCHARIFICATION OF ALFALFA, REED CANARYGRASS, AND SWITCHGRASS
- (Peer Reviewed Journal)
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| Dien, B.S., Jung, H.G., Vogel, K.P., Casler, M.D., Lamb, J.F., Iten, L.B., Mitchell, R., Sarath, G. 2006. Chemical composition and response to dilute-acid pretreatment and enzymatic saccharification of alfalfa, reed canarygrass, and switchgrass. Biomass and Bioenergy. 30:880-891. |
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ENZYMATIC SACCHARIFICATION OF HOT-WATER PRETREATED CORN FIBER FOR PRODUCTION OF MONOSACCHARIDES
- (Peer Reviewed Journal)
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| Dien, B.S., Li, X., Iten, L.B., Jordan, D.B., Nichols, N.N., O Bryan, P.J., Cotta, M.A. 2006. Enzymatic saccharification of hot-water pretreated corn fiber for production of monosaccharides. Enzyme and Microbial Technology. 39:1137-1144. |
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HETEROLOGOUS PRODUCTION BY TRICHODERMA REESEI OF A XYLANASE OF THE ANAEROBIC FUNGUS ORPINOMYCES PC-2
- (Abstract Only)
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| (09-Nov-05) |
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ACTIVE-SITE MODELS OF RIBOFLAVIN SYNTHASE
- (Proceedings)
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| Jordan, D.B., Calabrese, J.C., Liao, D., Wawrzak, Z., Zheng, Y. 2005. Active-site models of riboflavin synthase. In: Nishino, T., Miura, R., Tanokura, M., Fukui, K., editors. Flavins and Flavoproteins 2005. Tokyo: ARchiTect, Inc. p. 737-742. |
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DEVELOPMENT OF PLASMIDS FOR EXPRESSION OF HETEROLOGOUS PROTEINS IN RHIZOPUS ORYZAE
- (Abstract Only)
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| Mertens, J.A., Skory, C.D. 2005. Development of plasmids for expression of heterologous proteins in Rhizopus oryzae [abstract]. Society of Industrial Microbiology. p. 100. |
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ETHANOL FERMENTATION OF SUGARS IN CORN STOVER DILUTE ACID HYDROLYSATES
- (Abstract Only)
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| Nichols, N.N., Dien, B.S., Cotta, M.A. 2005. Ethanol fermentation of sugars in corn stover dilute acid hydrolysates [abstract]. Society of Industrial Microbiology. p. 85. |
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USE OF FIELD PEA STARCH AS A FEEDSTOCK FOR ETHANOL FERMENTATION
- (Abstract Only)
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| Nichols, N.N., Dien, B.S., Wu, V., Cotta, M.A. 2005. Use of field pea starch as a feedstock for ethanol fermentation [abstract]. International Starch Technology. p. 96. |
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ENZYMATIC SACCHARIFICATION OF PRETREATED CORN FIBER FOR PRODUCTION OF SUGARS
- (Abstract Only)
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| Dien, B.S., Li, X., Jordan, D.B., Nichols, N.N., Iten, L.B., Cotta, M.A. 2005. Enzymatic saccharification of pretreated corn fiber for production of sugars [abstract]. International Starch Technology. p. 90. |
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THE CORN ETHANOL INDUSTRY
- (Book / Chapter)
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| Nichols, N.N., Dien, B.S., Bothast, R.J., Cotta, M.A. 2006. The corn ethanol industry. In: Minteer, S., editor. Alcoholic Fuels. Boca Raton, FL: CRS Press. p. 59-78. |
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HYDROLYSIS AND FERMENTATION OF PERICARP AND ENDOSPERM FIBER RECOVERED FROM ENZYMATIC CORN DRY GRIND PROCESS
- (Peer Reviewed Journal)
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| Dien, B.S., Johnston, D., Hicks, K.B., Cotta, M.A., Singh, V. 2005. Hydrolysis and fermentation of pericarp and endosperm fibers recovered from enzymatic corn dry-grind process. Cereal Chemistry. 82(5):616-620. |
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ETHANOL FERMENTATION OF STARCH FROM FIELD PEAS
- (Peer Reviewed Journal)
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| Nichols, N.N., Dien, B.S., Wu, Y., Cotta, M.A. 2005. Ethanol fermentation of starch from field peas. Cereal Chemistry. 82(5):554-558. |
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ENZYMATIC SACCHARIFICATION OF PRETREATED CORN FIBER FOR PRODUCTION OF SUGARS
- (Abstract Only)
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| Dien, B.S., Li, X., Cotta, M.A. 2005. Enzymatic saccharification of pretreated corn fiber for production of sugars [abstract]. Biotechnology for Fuels and Chemicals. Paper No. B-36. |
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BUTANOL PRODUCTION FROM AGRICULTURAL RESIDUES: IMPACT OF DEGRADATION PRODUCTS ON CLOSTRIDIUM BEIJERINCKII GROWTH AND BUTANOL FERMENTATION
- (Abstract Only)
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| Ezeji, T., Qureshi, N., Blaschek, H.P. 2005. Butanol production from agricultural residues: impact of degradation products on Clostridium beijerinckii growth and butanol fermentation [abstract]. World Congress on Industrial Biotechnology and Bioprocessing. p. 163. |
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ACTIVE-SITE MODELS OF RIBOFLAVIN SYNTHASE
- (Abstract Only)
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| Jordan, D.B., Calabrese, J.C. 2005. Active-site models of riboflavin synthase [abstract]. International Symposium on Flavins and Flavoproteins. p. 104. |
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SUBSTRATE SPECIFICITIES OF TWO GLYCOSIDE HYDROLASE FAMILY 11 XYLANASES
- (Abstract Only)
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| Li, X., Jordan, D.B., Cotta, M.A. 2005. Substrate specificities of two glycoside hydrolase family 11 xylanases [abstract]. American Chemical Society. Paper No. 066. |
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REVIEW OF PROCESS FOR PRODUCING CORN FIBER OIL AND ETHANOL FROM "QUICK FIBER"
- (Review Article)
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| Dien, B.S., Nagle, N., Singh, V., Moreau, R.A., Tucker, M.P., Nichols, N.N., Johnston, D., Cotta, M.A., Hicks, K.B., Nguyen, Q., Bothast, R.J. 2005. Review of process for producing corn fiber oil and ethanol from "Quick Fiber." International Sugar Journal. 107(1275):187-191. |
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