Location: Food and Feed Safety Research
2007 Annual Report
Under a second Non-Funded Cooperative Agreement between ARS and LSU (6435-41000-097-02N) research was conducted to discover novel fungicides produced by Asian plants. One experimental protocol initially tests crude plant extracts for fungicidal properties. One such extract, code name ARW, was found to contain a potent fungicide.
a) CAY-1 synergy studies, status of other tested plant fungicides. -- In our lab, we discovered that silver is a potent fungicide, but its activity against Aspergillus and Fusarium is enhanced with pure CAY-1 (our patented fungicide from cayenne pepper). Ethylene diamine tetraacetic acid (EDTA) was not synergistic with CAY-1 against these fungi. Bay leaf extracts contain a potent fungicide that is active against Fusarium. We have semipurified the compound, but final purification was not achieved. (National Program 306, Component 1, Problem statement 1.1.1; Crude extraction and initial determination of active agent; and Problem statement 1.1.2; Purification of fungicidal compound)
b) CAY-1 collaborative studies. -- Our patented plant fungicide, CAY-1, continues to be studied both in our lab and that of collaborators. Initial studies by USDA, ARS collaborators (Mosquito and Fly Research Unit, Gainesville, FL) showed that pure CAY-1 kills mosquito larva species (weak activity against Aedes aegypti and Culex quinquefasciatus; strong activity against Anopheles albimanus), but does not kill A. aegypti adults. Our crude bay leaf extract, which has fungicidal properties, was also found to kill A. aegypti mosquito adults. Collaborators at Children’s Hospital (New Orleans) initiated studies of CAY-1 efficacy against the human pathogens, Cryptococcus and Candida species. (National Program 306, Component 2, Determination of commercial potential of discovered antifungal compounds)
De Lucca II, A.J. 2007. Harmful fungi in both agriculture and medicine. Revista Iberoamericana De Micologia. 24:3-13.
De Lucca II, A.J., Bland, J.M., Boue, S.M., Vigo, C.B., Cleveland, T.E., Walsh, T.J. 2006. Synergism of Cay-1 with amphotericin B and itraconazole. Chemotherapy. 52:285-287.