2008 Annual Report
Nutrient Component Objective 1: Develop management practices and decision tools for long-term use of animal manure as an alternative source of fertilizer for forages and row crops with regard to the following factors: Impacts on crop yield, nutrient loading, availability and uptake, application rate and timing, tillage, methods of application, soil quality, and soil carbon sequestration and greenhouse gas emissions. Objective 2: Determine if nutrient loading from agricultural watersheds in karst terrain is a function of physical watershed characteristics.
Emission Component Objective 3: Reduce odiferous emissions by developing innovative molecular-based methods to identify and quantify microorganisms and biological activities responsible for production of odorous compounds in livestock wastes. Objective 4: Develop new analytical approaches to quantify gases (e.g. methane, H2S), volatile odor compounds (e.g. p-cresol, skatole, and other VOCs) and evaluate treatment technologies for odor abatement at animal production facilities and manure-applied fields. Pathogen Component Objective 5: Employ molecular-based methods to improve detection, quantification, and evaluation of transport, and survival of pathogens including Salmonella and E. coli O157:H7 from animal manure. Also, compare survival of these pathogens with indicator organisms through a series of laboratory and watershed studies.
It has been reported that over 11.4 million tons of broiler litter was generated in 1996, of which over 90% was land applied. With regard to permanent pasture systems, inability to incorporate waste materials leads to increased nutrient concentration, such as P, Cu, and Zn near the soil surface. We studied the impact of pasture management and broiler litter application rate on runoff nutrient concentration. The inorganic N content of the runoff exiting grazed or grazed/hayed combination paddocks with litter applied on N basis was greater than the same treatments that received litter on P basis. All the treatments that received broiler litter on N basis had greater total P and total dissolved P in the runoff water. The mean total P loss per runoff event ranged from 7 to 45 g ha-1, and the grazed treatment with litter applied on N basis had the most. Total dissolved P was the dominant P fraction in the runoff ranging from 85% to 93% of the total P. Our results indicate that litter should be applied on N basis if the soil P is low and the pasture is used for hay production. In contrast, litter should be applied on P basis if pasture is being used for grazing.
The accomplishment addresses Problem Area 4 -- Farming Systems and Practices for Efficient and Balanced Manure Nutrient Management -- of the Nutrient Management Component under NP 206.2. Broiler Chicken Litter Application Timing Effect on Coastal Bermudagrass in Southeastern U.S:
In many states swine effluent application timing is regulated and limited to spring and summer months. In contrast, poultry litter application timing is not regulated; hence it is being surfaced land applied year-round in many states. It is expected that soon, litter application during the winter months or rainy months will be prohibited. Therefore, producers have to store litter for application during spring and summer. In this study, we attempted to identify an optimum date or dates for litter application that maximizes nutrient availability and uptake to crops and minimizes nutrient buildup in soil or losses through runoff. All the litter application dates did not influenced coastal bermudagrass dry matter yield and nutrient uptake except for nitrogen. The residual buildup of carbon, which is a beneficial impact, and the phosphorus, cupper, and zinc with potential negative impact were observed with all litter application timings. We were expecting that late summer and early fall litter application to bermudagrass would be less efficient than early spring and early summer application dates, but high soil fertility level at the study site and climatic factors may have impacted the costal bermudagrass growth, which confounded the real response to litter application timing.
The accomplishment addresses Problem Area 4 -- Farming Systems and Practices for Efficient and Balanced Manure Nutrient Management -- of the Nutrient Management Component under NP 206.3. Identification of Odor Producing Microorganisms in Swine Wastes.
Hydrogen sulfide, produced by sulfate reducing bacteria (SRB), is one of the most potent malodors emitted from anaerobic swine waste storage systems. However, little is known about the prevalence and diversity of SRB in those systems. In this study, the SRB population in swine manure storage systems was characterized and four new quantitative, real-time polymerase chain reaction (PCR) assays were developed to target SRB in swine slurry. To date, this is the only study to evaluate the phylogeny and concentration of SRB in any livestock waste storage system.
This research addresses Problem Area 1 (Understanding the Biological, Chemical, and Physical Mechanisms Affecting Emissions) of Atmospheric Emissions Component of the National Program 206 - Manure and Byproduct Utilization.4. Laboratory and Field Evaluation of Broiler Litter Nitrogen Mineralization:
Broiler litter, a mixture of manure and bedding materials, is used widely as fertilizer on pasture and row crop lands in poultry producing regions. Most of the land applied litter is broadcasted on the pasture lands or incorporated for some row crops. Accurate estimation of litter nutrients is required for efficient utilization of this resource in agriculture. It is difficult to predict the availability of litter nitrogen (N) to plants since both N transformation (conversion of organic N to inorganic N) and losses of N influence availability. Limited evidence suggests that soil properties may affect the amount of net N availability from broiler litter. Two studies were conducted: first, a laboratory incubation study to quantify poultry litter N availability at two temperatures (18° and 25° C), two soil moisture regimes, constant and fluctuating, and three soil types, Brooksville silty clay loam and Ruston sandy loam from Mississippi, and Catlin silt loam from Illinois; second, a field study using only Brooksville and Ruston soil to validate the laboratory results, using similar soils in microplot cylinders and anion exchange resin to capture available N. The impact of temperature was significant, while soil moisture regimes had no significant impact on litter-derived inorganic N. Field results followed the same trend as the laboratory study but with much lower rate.
The accomplishment addresses Problem Area 4 -- Farming Systems and Practices for Efficient and Balanced Manure Nutrient Management -- of the Nutrient Management Component under NP 206.5. Understanding the role starvation status plays on the adhesion and transport properties of Escherichia coli in saturated porous media.
Escherichia coli (E. coli) is commonly used as an indicator of fecal pollution. Therefore, it is important to have a good understanding of how this microorganism moves in the environment. Cell surface characteristics such as surface charge and hydrophobicity have been shown to play an important role controlling microbial movement in the environment. What is not well known is how these surface characteristics are affected by the starvation status of the cells. Research showed that surface properties and transport behavior of E. coli depended strongly on the starvation status of the cells. This study highlights the importance of consistency in bacterial preparation for transport and characterization studies and has considerable implications for the future evaluation and prediction of E. coli fate in subsurface environments.
This research supports Focus Area 2a of the Pathogens Component of NP 206 – Inactivation Rates and Transport Characteristics of Pathogens in Animal Agriculture.6. Equilibrium Sampling Used to Monitor Malodors in a Swine Waste Lagoon.
The concentrations of malodorous compounds in an anaerobic swine waste lagoon were monitored during the late summer to late fall of 2006. While the lagoon was being pumped down, malodorous compounds fluctuated widely, then increased greatly as compared to the period before pumping, and continued to increase as fall progressed. Suspended solids, volatile suspended solids, and total organic carbon increased near the bottom of the lagoon during this same period.
This research addresses Problem Area 1 (Understanding the Biological, Chemical, and Physical Mechanisms Affecting Emissions) of Atmospheric Emissions Component of the National Program 206 - Manure and Byproduct Utilization.7. Effect of Stratification and Seasonal Variability on the Profile of an Anaerobic Swine Waste Treatment Lagoon.
An understanding of the nature of wastes in an anaerobic swine lagoon is essential in the design and operation of alternative collection, treatment, and disposal facilities for environmental quality management such as odor control, nutrient and pathogen reduction. In this study, the characterization of an anaerobic swine waste treatment lagoon from a farrowing operation (~2000 sows) was carried out to examine the dynamics of the system due to stratification and seasonal variability. The sampling process was carried out from spring to fall season. The pH and temperature were monitored and recorded continuously from the epilimnion (top) and hypolimnion (bottom) layers of the lagoon. Results showed that nutrient (C, N, P, S) concentrations varied according to stratified lagoon layers and season. Trace minerals such as Al, Ca, Fe, K, Na, and Mg, on the other hand, appeared to be affected more by stratification than seasonal variability. The reason for the decrease in nutrient concentrations in summer time may be due to increase microbial activities which required more essential nutrients (i.e., C, N, P, S) rather than trace minerals for growth during active season. Microbial community structure appeared to be affected by the stratification and seasonal variability. The microbial community appeared to be more diverse in the spring time than late summer or early fall. Based on these data, it is important to consider the effect of stratification and seasonal variability of waste loading from traditional anaerobic swine lagoon when designing and operating an alternative anaerobic digester.
This research addresses Problem Area 1 (Understanding the Biological, Chemical, and Physical Mechanisms Affecting Emissions) of Atmospheric Emissions Component of the National Program 206 - Manure and Byproduct Utilization.
Bolster, C.H., Hornberger, G.M. 2007. On the use of Linearized Langmuir Equations. Soil Science Society of America Journal. Vol.71 Issue 6 P 1796-1806
Sistani, K.R., Brink, G.E., Oldham, J.L. 2008. Managing Broiler Litter Application Rate and Grazing to Decrease Watershed Runoff Losses. Journal of Environmental Quality. 37:718-724
Sistani, K.R., Adeli, A., Tewolde, H., Brink, G.E. 2007 Broiler chicken litter application timing effect on coastal bermudagrass in southeastern u.s.. Nutrient Cycling in Agroecosystems. 81:49-57
Sistani, K.R., Adeli, A., McGowen, S.L., Tewolde, H., Brink, G.E. 2008. Laboratory and field evaluation of broiler litter nitrogen meralization. Bioresource Technology. 99:2603-2611
Sistani, K.R., Rasnake, M., Sikora, F. 2008. Poultry litter and tillage influence on corn production and soil nutrients in a Kentucky silt loam soil. Soil & Tillage Research. 98:130-139.
Warren, J.G., Penn, C.J., Mcgrath, J.M., Sistani, K.R. 2008. The Impact of Alum Additions on Organic P Transformations in Poultry Litter and Litter Amended Soil. Journal of Environmental Quality. 37:469-476
Griffin, T.S., Honeycutt, C.W., Albrecht, S.L., Sistani, K.R., Torbert Iii, H.A., Wienhold, B.J., Woodbury, B.L., Hubbard, R.K., Powell, J.M. 2008. Nationally coordinated evaluation of soil nitrogen mineralization rate using a standardized aerobic incubation protocol. Communications in Soil Science and Plant Analysis. 39:257-268.
Cook, K.L., Whitehead, T.R., Spence, C., Cotta, M.A. 2008. Evaluation of the sulfate-reducing bacterial population associated with stored swine slurry. Anaerobe. 14:172-180.
Haznedaruglu, B.Z., Bolster, C.H., Walker, S.L. 2008. The Role of Starvation on Escherichia coli Adhesion and Transport in Saturated Porous Media. Water Research. Vol 42 pp 1547-1554
Rothrock Jr, M.J., Cook, K.L., Lovanh, N.C., Warren, J.G., Sistani, K.R. 2008. Development of a Quantitative Real-Time Polymerase Chain Reaction Assay to Target a Novel Group of Ammonia-Producing Bacteria Found in Poultry Litter . Poultry Science. 87(6):1058-1067
Adeli, A., Bolster, C.H., Rowe, D.E., McLaughlin, M.R., Brink, G.E. 2008. Effects of long-term swine effluent application on selected soil properties. Soil Science. 173:223-235.