2005 Annual Report
The research to be undertaken falls under National Program 107 - Human Nutrition and addresses performance goal 3.1.2.B as described in the National Program Action Plan. Goal 3.1.2 is "Food Composition and Consumption: Develop techniques for determining food composition, maintain national food composition databases, monitor the food and nutrient consumption of the U.S. population, and develop and transfer effective nutrition intervention strategies." This project directly addresses Priority Objective B. Develop Analytical Methodology for Nutrients.
The field of nutrition and public health will benefit, in general, from an improved nutrition database for trace metals and increased knowledge regarding the concentrations and species of trace metals in foods. The field of analytical chemistry will benefit from improved methods for.
Ultratrace Methods *Develop GF-AAS and ICP methods for Co and Cr.
Cobalamin Speciation *Develop method for cobalamin species using capillary electrophoresis and ICP-MS. *Start development of sample preparation method for cobalamins focusing on solid phase extraction.
Fe Speciation *Acquire standards for Fe species. *Initiate development of an High Performance Liquid Chromatography (HPLC) separation method for Fe species extracted from meat.
Year 2 (FY 2005) Robust Multielement Methods *Complete evaluation of questionnaire. *Develop in-house food control materials for evaluation by food industry labs.
Ultratrace Methods *Complete development of GF-AAS and ICP methods for Co and Cr. *Initiate development of GF-AAS and ICP methods for Ni, and V.
Cobalamin Speciation *Complete development of sample preparation method for cobalamins.
Fe Speciation *Continue development of HPLC method for Fe species extracted from meat. *Determine effect of cooking and acidification on heme Fe. *Set up in vitro digestion system to study heme Fe. *Initiate study of bioavailability of heme Fe.
Year 3 (FY 2006) Robust Multielement Methods *Initiate development of ICP methods for food materials.
Ultratrace Methods *Complete development of methods for Ni, and V. *Initiate development of in-house control materials for Co, Cr, Ni, and V.
Cobalamin Speciation *Initiate determination of cobalamins in foods and supplements. *Develop a fresh frozen quality control materials.
Fe Speciation *Continue study with in vitro digestion system for heme Fe. *Continue study of bioavailability of heme Fe. *Initiate quantification of total Fe and heme Fe in foods for database.
Year 4 (FY 2007) Robust Multielement Methods *Develop Association Of Analytical Chemists (AOAC) method(s) for determination of food materials using ICP.
Ultratrace Methods *Complete in-house control materials for Co, Cr, Ni, and V. *Initiate determination of Co, Cr, Ni, and V in food materials.
Cobalamin Speciation *Initiate development of a dietary supplement quality control material for cobalamins.
Fe Speciation *Finish study with in vitro digestion system for heme Fe. *Finish study of bioavailability of heme Fe. *Determine total Fe and heme Fe in foods for database.
Year 5 (FY 2008) Robust Multielement Methods *Complete AOAC method(s) for determination of food materials using ICP.
Ultratrace Methods *Complete determination of Co, Cr, Ni, and V in food materials
Cobalamin Speciation *Complete development of dietary supplement quality control material for cobalamins.
Fe Speciation *Determine total Fe and heme Fe in foods for database.
Collaborated with the Plant, Soil, and Nutrition Lab in Ithaca, NY, on evaluation of cooking on the bioavailability. Bile juices are critical to solubilizing heme Fe after denaturation of the myoglobin after cooking.
Developed and circulated a questionnaire to colleagues from the National Food Processor's Analytical Chemists Subcommittee regarding low level trace element methods. Information gathered includes: methods used for trace element determinations, quality control strategies, interest in fresh food QC materials, and interest in participation in a study to improve low level trace element determinations for Mn, Zn, Fe and Cu. All of the laboratories contacted are commercial analytical labs. Many are associated with food producing companies and some are contract labs but all of them generate food composition data. The expected outcome of the study is development and technology transfer of robust methods suitable for industrial labs for the production of multielement food composition data. Another product is the production of much needed in-house food QC materials.
Participated in an interlaboratory collaborative study determining nine trace elements (Ca. Cu, Fe, Na, K, P, Mg, Zn, and Mn) in a nutritional liquid (similar to Ensure) and a cereal (similar to Cheerios). We were 1 of 22 laboratories who participated and our data were in good agreement with the mean values from the study.
Developed method for heme Fe in meats that employed the classic Hornsey extraction (acidified acetone) and determined the extracted Fe using atomic absorption spectrometry. Demonstrated that the use of spectrophotometry for the determination of Fe in the Hornsey extract gave results that are biased high by 15% to 40%. Initiated development of HPLC method to separate components extracted by the Hornsey method to verify that all the Fe was heme FE.