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United States Department of Agriculture

Agricultural Research Service

Richard H. Shukle (Rich)

Research Entomologist


Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Cloning, characterization, expression analysis and inhibition studies of a novel gene encoding Bowman-Birk type protease inhibitor from rice bean - (Peer Reviewed Journal)
Katoch, R., Singh, S.K., Thakur, N., Dutt, S., Yadav, S.K., Shukle, R.H. 2014. Cloning, characterization, expression analysis and inhibition studies of a novel gene encoding Bowman-Birk type protease inhibitor from rice bean. Gene. 546:342-351.
A genome-wide survey of small interfering RNA and micro RNA pathway genes in a galling insect - (Peer Reviewed Journal)
Shreve, J.T., Shukle, R.H., Subrqamanyam, S., Johnson, A.J., Williams, C.E., Schemerhorn, B.J., Stuart, J.J. 2013. A genome-wide survey of small interfering RNA and micro RNA pathway genes in a galling insect. Journal of Insect Physiology. 59:367-376.
Biological and molecular characterization of Hessian fly (Diptera: Cecidomyiidae) from Israel - (Peer Reviewed Journal)
Johnson, A.J., Weintraub, P., Schemerhorn, B.J., Shukle, R.H., Katoch, R. 2012. Biological and molecular characterization of Hessian fly (Diptera: Cecidomyiidae) from Israel. Bulletin of Entomological Research. 102:632-643.
Effects of antinutrient proteins on Hessian fly (Diptera: Cecidomyiidae) larvae - (Abstract Only)
Shukle, R.H., Subramanyam, S., Williams, C.E. 2012. Effects of antinutrient proteins on Hessian fly (Diptera: Cecidomyiidae)larvae [abstract]. In: Proceedings of the 20th Biennial International Plant Resistance to Insect Workshop. April 1-4, 2012, Minneapolis, Minnesota. p. 25.
Gall midges (hessian flies) as plant pathogens Reprint Icon - (Review Article)
Stuart, J.J., Chen, M., Harris, M.O., Shukle, R.H. 2012. Gall midges (hessian flies) as plant pathogens. Annual Review of Phytopathology. 50:17.1-17.19.
Effects of antinutrient proteins on Hessian fly (Diptera: Cecidomyiidae) larvae - (Peer Reviewed Journal)
Shukle, R.H., Subramanyam, S., Williams, C.E. 2011. Effects of antinutrient proteins on Hessian fly (Diptera: Cecidomyiidae) larvae. Journal of Insect Physiology. 58:41-48.
Translational insect genomics: pheromone traps and molecular markers genotype Hessian flies for virulence to resistance gene H13 in wheat Reprint Icon - (Abstract Only)
Johnson, A.J., Buntin, G., Flanders, K., Reay-Jones, F., Reisig, D., Schemerhorn, B.J., Stuart, J.J., Shukle, R.H. 2022. Translational insect genomics: pheromone traps and molecular markers genotype Hessian flies for virulence to resistance gene H13 in wheat. Plant Genomics Series. 78:30.
Induced epidermal permeability modulates resistance and susceptibility of wheat seedlings to herbivory by Hessian fly larvae - (Peer Reviewed Journal)
Williams, C.E., Shukle, J.T., Nemacheck, J.A., Subramanyam, S., Saltzmann, K., Shukle, R.H. 2011. Induced epidermal permeability modulates resistance and susceptibility of wheat seedlings to herbivory by Hessian fly larvae. Journal of Experimental Botany. 63:4521-4531.
Cloning and characterization of mariner-like elements in the soybean aphid, Aphis glycines Matsumura Reprint Icon - (Peer Reviewed Journal)
Mittapalli, O., Rivera-Vega, L., Bhandary, B., Bautista, M.A., Mamidala, P., Michel, A.P., Shukle, R.H., Mian, R.M. 2011. Cloning and characterization of mariner-like elements in the soybean aphid, Aphis glycines Matsumura. Bulletin of Entomological Research. 102(6):697-704.
Effects of antinutrient and toxic proteins on Hessian fly (Diptera: Cecidomyiidae) larvae: Potential for transgenic resistance in wheat to complement native resistance - (Abstract Only) - (26-Mar-11)
Annotation of metabolic and biosynthesis genes from Hessian fly (Diptera: Cecidomyiidae) - (Abstract Only) - (26-Mar-11)
Genotyping virulence to H13 wheat in field collections of Hessian fly from the southeastern United States - (Abstract Only) - (26-Mar-11)
Use of a nuclear marker to assess population structure in Hessian fly (Mayetiola destructor) - (Peer Reviewed Journal)
Johnson, A.J., Morton, P.K., Schemerhorn, B.J., Shukle, R.H. 2011. Use of a nuclear marker to assess population structure in Hessian fly (Mayetiola destructor). Annals of the Entomological Society of America. 104:666-674.
Toxin Production as a Wheat Defense Strategy against Hessian fly - (Abstract Only)
Williams, C.E., Shukle, R.H., Subramanyam, S. 2011. Toxin Production as a Wheat Defense Strategy against Hessian fly. Plant and Animal Genome Conference Proceedings. http:www.intl-pag.org/.
Unconventional conservation among genes encoding small secreted salivary sland proteins from a gall midge Reprint Icon - (Peer Reviewed Journal)
Chen, M., Liu, X., Zhao, H., Stuart, J.J., Shukle, R.H., Yang, Z., Hulbert, S. 2010. Unconventional conservation among genes encoding small secreted salivary sland proteins from a gall midge. BMC Evolutionary Biology. 10:296.
Gene characterization of two digestive serine proteases in orange blossom wheat midge (Sitodiplosis mosellana) - (Peer Reviewed Journal)
Arrueta, L., Shukle, R.H., Wise, I.L., Mitapalli, O. 2011. Gene characterization of two digestive serine proteases in orange blossom wheat midge (Sitodiplosis mosellana). The Canadian Entomologist. 142:(6)532-545.
Virulence in Hessian fly (Diptera: cecidomyiidae) field collections from the Southeastern United States to twenty-one resistance (R) genes in wheat - (Peer Reviewed Journal)
Cambron, S.E., Buntin, G.D., Weisz, R., Holland, J.D., Flanders, K.L., Schemerhorn, B.J., Shukle, R.H. 2010. Virulence in Hessian fly (Diptera: cecidomyiidae) field collections from the Southeastern United States to twenty-one resistance (R) genes in wheat. Journal of Economic Entomology. 103:229-2235.
Assessment of Structural Variation and Molecular Mapping of Insertion Sites of Desmar-like Elements in the Hessian Fly Genome - (Peer Reviewed Journal)
Behura, S.K., Shukle, R.H., Stuart, J.J. 2010. Assessment of Structural Variation and Molecular Mapping of Insertion Sites of Desmar-like Elements in the Hessian Fly Genome. Insect Molecular Biology. 19(6):707-715.
The Gut Transcriptome of the Hessian Fly (Mayetiola Destructor), a Member of the Gall Midges Reprint Icon - (Peer Reviewed Journal)
Zhang, S., Shukle, R. H., Mittapalli, O., Zhu, Y. C., Reese, J. C., Wang, H., Hua, B.Z., Chen, M.S. 2010. The gut transcriptome of a gall midge, Mayetiola destructor. Journal of Insect Physiology. 56:1198-1206.
Ultrastructural Changes in the Midgut of Hessian Fly Larvae Feeding on Resistant Wheat Reprint Icon - (Peer Reviewed Journal)
Shukle, R.H., Subramanyam, S., Williams, C.E. 2010. Ultrastructural Changes in the Midgut of Hessian Fly Larvae Feeding on Resistant Wheat. Journal of Insect Physiology. 56:754-760.
Ultrastructural and Transcriptional Changes in Response to Dietary Lectins in the Hessian Fly Larval Midgut - (Abstract Only)
Shukle, R.H., Subramanyam, S., Williams, C.E. 2009. Ultrastructural and Transcriptional Changes in Response to Dietary Lectins in the Hessian Fly Larval Midgut. Entomological Society of America Annual Meeting. Available: http://esa.confex.com/esa/2009/webprogram/Paper43341.html.
Plant Lectins: Wheat Defense Strategy Against Hessian Fly - (Abstract Only)
Subramanyam, S., Shukle, R.H., Williams, C.E. 2009. Plant Lectins: Wheat Defense Strategy Against Hessian Fly. Entomological Society of America Proceedings. Available: http://esa.confex.com/esa/2009/webprogram/Paper43341.html.
Hessian Fly-Induced Resistance and Susceptibility in Wheat - (Abstract Only) - (16-Jul-09)
Ultrastructural Responses in the Midgut of Hessian Fly Larvae Feeding on Resistant Wheat - (Abstract Only) - (15-Feb-09)
Analyzing the diversity of secreted salivary gland transcripts in Hessian fly populations from Israel and the United States - (Abstract Only) - (15-Feb-09)
Characterization and expression analysis of a gene encoding a secreted lipase-like protein expressed in the salivary glands of larval Hessian fly, Mayetiola destructor (Say) - (Peer Reviewed Journal)
Shukle, R.H., Mittapalli, O., Morton, P.K., Chen, M. 2009. Characterization and expression analysis of a gene encoding a secreted lipase-like protein expressed in the salivary glands of larval Hessian fly, Mayetiola destructor (Say). Journal of Insect Physiology. 55(2):104-111.
Ultrastructural changes and transcriptional responses to resistant wheat and starvation in the midgut of Hessian fly larvae - (Abstract Only)
Shukle, R.H., Subramanyan, S., Omprakash, M., Williams, C.E. 2008. Ultrastructural changes and transcriptional responses to resistant wheat and starvation in the midgut of Hessian fly larvae. Annals of the Entomological Society of America. Available: http://esa.confex.com/esa/2008/webprogram/Paper37700.html.
Genetic Variation of Hessian Fly Populations from Manitoba Canada - (Abstract Only)
Shreve, J.J., Morton, P.K., Wise, I.L., Shukle, R.H., Schemerhorn, B.J. 2008. Genetic Variation of Hessian Fly Populations from Manitoba Canada. Annals of the Entomological Society of America. Available: http://esa.confex.com/esa/2008/webprogram/Paper37737.html.
Molecular characterization and responsive expression of a defender against apoptotic cell death homologue from Hessian fly, Mayetiola destructor Reprint Icon - (Peer Reviewed Journal)
Mittapalli, O., Shukle, R.H. 2008. Molecular characterization and responsive expression of a defender against apoptotic cell death homologue from Hessian fly, Mayetiola destructor. Comparative Biochemistry and Physiology. Part B 149: 517-523.
Tissue and developmental expression of a gene from Hessian fly encoding an ABC-active-transporter protein during interactions with wheat Reprint Icon - (Peer Reviewed Journal)
Shukle, R.H., Yoshiyama, M., Morton, P.K., Schemerhorn, B.J. 2007. Tissue and developmental expression of a gene from Hessian fly encoding an ABC-active-transporter protein during interactions with wheat. Journal of Insect Physiology. 55:146-154.
Hessian fly (Mayetiola destructor) larval midgut and salivary gland morphology: Light and electron microscopy studies - (Abstract Only)
Shukle, R.H. 2007. Hessian fly (Mayetiola destructor) larval midgut and salivary gland morphology: Light and electron microscopy studies. Annuals of the Entomological Society of America. Paper No. DO486.
Comparison of gene expression in the salivary glands of three major insect pests of cereals - (Abstract Only)
Mittapalli, O., Shukle, R.H., Bentur, J., Neal, J., Stuart, J., Chen, M., Wise, I. 2007. Comparison of gene expression in the salivary glands of three major insect pests of cereals. Annuals of the Entomological Society of America. Paper No. D0507.
Antioxidant Defense Response in a Galling Insect Reprint Icon - (Peer Reviewed Journal)
Mittapalli, O., Neal, J.J., Shukle, R.H. 2007. Antioxidant defense response in a galling insect. Proceedings of the National Academy of Sciences. 104(6):1889-1894.
Hessian fly larval attack induces cell permeability in wheat - (Abstract Only)
Subramanyam, S., Saltzmann, K.D., Shukle, J., Shukle, R.H., Williams, C.E. 2007. Hessian fly larval attack induces cell permeability in wheat. [abstract] Plant and Animal Genome VX Conference Abstracts, San Diego, CA, Jan. 13-17, 2007.
Tissue and Life State Specificity of Glutatione S-Transferase Expression in the Hessian Fly, Mayetiola Destructor: Implications for Resistance to Host Allelochemicals Reprint Icon - (Peer Reviewed Journal)
Mittapalli, O., Neal, J.J., Shukle, R.H. 2007. Tissue and life state specificity of glutatione s-transferase expression in the Hessian fly, Mayetiola destructor: implications for resistance to host allelochemicals. Journal of Insect Science. 7(20):1-13.
Pyramiding of Insecticidal Compounds for Control of the Cowpea Bruchid (Callosobruchus Maculates F.) - (Peer Reviewed Journal)
Tarver, M.R., Shade, R.E., Shukle, R.H., Moar, W.J., Muir, W.M., Murdock, L.M., Pittendrigh, B.P. 2007. Pyramiding of insecticidal compounds for control of the cowpea bruchid (Callosobruchus maculates f.). Pest Management Science. Available at http://www3.interscience.wiley.com/cgi-bin/jhome/68504529?SRETRY=0.
Cloning and characterization of a salivary digestive lipase from Hessian fly (Diptera: Cecidomyiidae) - (Abstract Only) - (02-Oct-06)
Cdna Cloning and Transcriptional Expression of a Peritorphin-Like Gene in the Hessian Fly, Mayetiola Destructor (Say) Reprint Icon - (Peer Reviewed Journal)
Omprakash, M., Sardesai, N., Shukle, R.H. 2007. CDNA cloning and transcriptional expression of a peritorphin-like gene in the Hessian fly, Mayetiola destructor (say). Archives of Insect Biochemistry and Physiology. 64:19-29.
Expression Patterns of Antibacterial Genes in the Hessian Fly Reprint Icon - (Peer Reviewed Journal)
Mittapalli, O., Nagesh, S., Williams, C.E., Shukle, R.H. 2006. Expression patterns of antibacterial genes in the Hessian fly. Journal of Insect Physiology. 52:1143-1152.
Antioxidant defense response in the Hessian fly (Diptera: Cecidomyiidae) - (Abstract Only)
Mittapalli, O., Shukle, R.H., Neal, J. 2006. Antioxidant defense response in the Hessian fly (Diptera: Cecidomyiidae). [Abstract]. National Entomological Society of America Annual Meeting.
Gene-for-Gene Defense of Wheat Against the Hessian Fly Lacks a Classical Oxidative Burst Reprint Icon - (Peer Reviewed Journal)
Giovanini, M.P., Puthoff, D.P., Nemacheck, J.A., Mittapalli, O., Saltzmann, K.D., Ohm, H., Shukle, R.H., Williams, C.E. 2006. Gene-for-gene defense of wheat against the Hessian fly lacks a classical oxidative burst. Molecular Plant Microbe Interactions. 19:1023-1033.
Assessing the Phylogenetics of Hessian Fly Using Mitochondrial and Nuclear Markers - (Abstract Only)
Johnson, A.J., Schemerhorn, B.J., Shukle, R.H. 2006. Assessing the phylogenetics of Hessian fly using mitochondrial and nuclear markers [abstract]. International Plant Resistance to Insects Workshop Abstracts & Proceedings. p. 41.
Response of Genes Expressed in the Larval Hessian Fly During Interactions with Wheat - (Abstract Only)
Shukle, R.H., Mittapalli, O., Neal, J.J. 2006. Response of genes expressed in the larval Hessian fly during interactions with wheat [abstract]. Plant and Animal Genome Conference. p.14.
Response of Genes Expressed in the Larval Hessian Fly During Interactions with Wheat - (Abstract Only)
Shukle, R.H., Mittapalli, O. 2006. Response of genes expressed in the larval Hessian fly during interactions with wheat. Plant and Animal Genome Conference Proceedings. Available at: www.intl-pag.org/14/abstracts/PAG14_W334.html.
Identification of Mariner-Like Elements from Sitodiplosis Mosellana (Diptera: Cecidomyiidae) - (Peer Reviewed Journal)
Mittapalli, O., Shukle, R.H., Wise, I.L. 2006. Identification of mariner-like elements in Sitodiplosis mosellana (Diptera: Cecidomyiidae). Canadian Entomologist. 138(2):138-146.
Characterization of a Serine Carboxypeptidase in the Salivary Glands and Fat Body of the Orange Wheat Blossom Midge, Sitodiplosis Mosellana (Diptera: Cecidomyiidae) Reprint Icon - (Peer Reviewed Journal)
Omprakash, M., Wise, I.L., Shukle, R.H. 2006. Characterization of a serine carboxypeptidase in the Salivary glands and fat body of the orange wheat blossom midge, Sitodiplosis mosellana (Diptera: Cecidomyiidae). Insect Biochemistry and Molecular Biology 36:154-160.
Differential Expression of Two Cytochrome P450 Genes in Compatible and Incompatible Hessian Fly/wheat Interactions Reprint Icon - (Peer Reviewed Journal)
Mittapalli, O., Neal, J.J., Shukle, R.H. 2005. Differential expression of two cytochrome p450 genes in compatible and incompatible hessian fly/wheat interactions. Insect Biochemistry and Molecular Biology. 35:981-989.
Molecular Cloning and Characterization of Two Digestive Serine Proteases from the Hessian Fly, Mayetiola Destructor - (Peer Reviewed Journal)
Mittapalli, O., Shukle, R.H. 2005. Molecular cloning and characterization of two digestive serine proteases from the hessian fly, mayetiola destructor. Insect Molecular Biology. 14(3):309-318.
A first assessment of mitochondrial DNA variation and geographic distribution of haplotypes in the Hessian fly (Diptera: Cecidomyiidae) Reprint Icon - (Peer Reviewed Journal)
Johnson, A.J., Schemerhorn, B.J., Shukle, R.H. 2004. A first assessment of mitochondrial DNA variation and geographic distribution of haplotypes in the Hessian fly (Diptera: Cecidomyiidae). Annals of the Entomological Society of America.97(5):940-048.
A First Assessment of Mitochondrial DNA Variation and Geographic Distribution of Haplotypes in the Hessian Fly (Diptera: Cecidomyiidae) - (Peer Reviewed Journal)
Johnson, A.J., Schemerhorn, B.J., Shukle, R.H. 2004. A first assessment of mitochondrial dna variation and geographic distribution of haplotypes in the hessian fly (diptera: cecidomyiidae). Annals of the Entomological Society of America. 97(5):940-948.
Molecular Cloning and Characterization of a Glutathione S-Transferase Gene from Hessian Fly (Diptera: Cecidomyiidae) Reprint Icon - (Peer Reviewed Journal)
Yoshyiama, M., Shukle, R.H. 2004. Molecular cloning and characterization of a glutathione s-transferase gene from hessian fly (diptera: cecidomyiidae). Annals of the Entomological Society of America. 97(6):1285-1293.
Molecular Cloning and Characterization of a Major Digestive Serine Protease from the First Larval Instar of Hessian Fly - (Abstract Only)
Mittapalli, O. and Shukle, R.H. 2004. Molecular cloning and characterization of a major digestive serine protease from the first larval instar of Hessian fly. North Central Branch Meeting of the Entomological Society of America, Kansas City, MO.
Revealing Lineages of Hessian Fly Populations in the United States and Worldwide - (Abstract Only)
Johnson, A.J., Schemerhorn, B.J. and Shukle, R.H. 2004. Revealing lineages of Hessian fly populations in the United States and worldwide. North Central Branch Meeting of the Entomological Society of America, Kansas City, MO Available: http://esa.ent.iastate.edu/confreg/?gridaction=viewonepresentation&year=2004&presnum=033
Geographic Distribution of Hessian Fly (Diptera: Cecidomyiidae) Mitochondrial Haplotypes in the United States - (Abstract Only)
Johnson, A.J., Shukle, R.H., Schemerhorn, B.J. 2003. Geographic distribution of hessian fly (diptera: cecidomyiidae) mitochondrial haplotypes in the united states. Entomological Society of America Annual Meeting North Central Branch. National Meeting of the Entomological Society of America, Cincinnati, OH. Available: http://esa.confex.com/esa/2003/techprogram/paper_11083.htm
Expression of Hessian Fly Genes During Compatible Interactions with Wheat - (Abstract Only)
Shukle, R.H., Johnson, A.J. 2003. Expression of hessian fly genes during compatible interactions with wheat. NCB-ESA North Central Branch Entomological Society of America.
Use of Double-Stranded Rna Interference to Assess the Role of a Gst-Like Gene in Hessian Fly on Its Interaction with Wheat - (Abstract Only)
Yoshiyama, M., Shukle, R.H. 2003. Use of double-stranded RNA interference to assess the role of a GST-like gene in hessian fly on its interaction with wheat. Plant and Animal Genome Conference Proceedings.
Hessian Fly, Mayetiola Destructor (Say)(diptera:cecidomyiidae) - (Popular Publication)
Shukle, R.H. 2004. Hessian fly, mayetiola destructor (say)(diptera:cecidomyiidae). Encyclopedia of Entomology. 1(1):27-31.
Mitochondrial DNA Sequence Divergence in Hessian Fly - (Proceedings) - (09-Dec-01)
Analysis of a Putative Glutathione S-Transferase Gene from Hessian Fly - (Proceedings) - (09-Dec-01)
The Potential Role of Protease Inhibitors and Parasitoids on the Populationdynamics of Sitotroga Cerealella (Lepidoptera: Gelechiidae - (Peer Reviewed Journal) - (04-Oct-01)
Mitochondrial DNA Variation in the Hessian Fly - (Proceedings) - (05-Feb-01)
Molecular and Cytological Characterization of An Actin Gene from Hessian Fly - (Peer Reviewed Journal) - (07-Jun-00)
Biology and Host-Plant Relationships of the Hessian Fly: Past and Current Research - (Book / Chapter) - (27-Jul-99)
Biology and Host-Plant Relationships of the Hessian Fly: Past and Current Research - (Proceedings)
Harris, M.O., Williams, C.E., Ratcliffe, R.H., Foster, S.P., Griffin, W., Kanno, H., Morris, B.D., Ohm, H.W., Pickering, R.A., Rani, U., Shukle, R.H., Stuart, J.J., Zeismann, J. 2004. Biology and host-plant relationships of the hessian fly: past and current research. Proceedings of the International Workshop on Plant Genetic Resources. p. 31-44.
An Sts Linked to Virulence in Hessian Fly to Resistance Gene H13 in Wheat - (Peer Reviewed Journal) - (15-May-98)
Genetic Analysis of Virulence in the Hessian Fly - (Peer Reviewed Journal) - (29-Jul-96)
Molecular and Cytological Analysis of a Mariner Transposon from Hessian Fly - (Peer Reviewed Journal) - (20-May-96)
Last Modified: 7/23/2010
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