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United States Department of Agriculture

Agricultural Research Service

Gregory D. Wiens (Greg)

Molecular Biologist


Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Biochemical reference intervals for Flavobacterium psychrophilum -resistant and -susceptible rainbow trout lines, and pathophysiological changes after experimental infection - (Peer Reviewed Journal) - (12-Jun-14)
Validation of linked QTL for bacterial cold water disease resistance and spleen size on rainbow trout chromosome Omy19 - (Peer Reviewed Journal)
Vallejo, R.L., Palti, Y., Liu, S., Marancik, D.P., Wiens, G.D. 2014. Validation of linked QTL for bacterial cold water disease resistance and spleen size on rainbow trout chromosome Omy19. Aquaculture. 432:139-143.
Integration of selective breeding and vaccination to improve disease resistance in aquaculture: Application to control bacterial cold water disease - (Proceedings)
Wiens, G.D., Leeds, T.D. 2014. Integration of selective breeding and vaccination to improve disease resistance in aquaculture: Application to control bacterial cold water disease. In: Proceedings of One Hundred and Seventeenth Annual Meeting of the United States Animal Health Association, October 17 - October 23, 2013, San Diego, California. p. 448-453.
QTL for bacterial cold water disease resistance and spleen size are located on rainbow trout chromosome Omy19 - (Abstract Only)
Wiens, G.D., Vallejo, R.L., Liu, S., Marancik, D.P., Palti, Y. 2014. QTL for bacterial cold water disease resistance and spleen size are located on rainbow trout chromosome Omy19 [abstract]. International Conference on Integrative Salmonid Biology. Paper No. 65.
Genetic improvement through selective breeding: Part of an integrated strategy to reduce disease loss and Antibiotic use - (Abstract Only)
Wiens, G.D., Lapatra, S.E., Marancik, D.P., Leeds, T.D. 2014. Genetic improvement through selective breeding: Part of an integrated strategy to reduce disease loss and Antibiotic use [abstract]. Annual Eastern Fish Health Workshop. A98.
Histopathologic changes in disease resistant and susceptible-line juvenile rainbow trout experimentally infected with Flavobacterium psychrophilum - (Peer Reviewed Journal) - (10-Mar-14)
Flavobacterium branchiophilum and F. succinicans associated with bacterial gill disease in rainbow trout Oncorhynchus mykiss (Walbaum) in water recirculation aquaculture systems - (Peer Reviewed Journal)
Good, C., Davidson, J., Wiens, G.D., Welch, T.J., Summerfelt, S. 2014. Flavobacterium branchiophilum and F. succinicans associated with bacterial gill disease in rainbow trout Oncorhynchus mykiss (Walbaum) in water recirculation aquaculture systems. Journal of Fish Diseases. DOI: 10.1111/jfd.12249.
Transfer of serum and cells from Yersinia ruckeri vaccinated double-haploid Hot Creek Rainbow trout into outcross F1 progeny elucidates mechanisms of vaccine-induced protection - (Peer Reviewed Journal)
Evenhuis, J., Wiens, G.D., Wheeler, P., Welch, T.J., Lapatra, S.E., Thorgaard, G. 2013. Transfer of serum and cells from Yersinia ruckeri vaccinated double-haploid Hot Creek Rainbow trout into outcross F1 progeny elucidates mechanisms of vaccine-induced protection. Developmental and Comparative Immunology. 44: 145-151. DOI: 10.1016/j.dci.2013.12.004.
Validation of linkage between BCWD resistance and spleen size QTL on Omy19 in rainbow trout: Pleiotropy versus linkage - (Abstract Only)
Vallejo, R.L., Palti, Y., Liu, S., Marancik, D.P., Welch, T.J., Leeds, T.D., Evenhuis, J., Rexroad III, C.E., Wiens, G.D. 2014. Validation of linkage between BCWD resistance and spleen size QTL on Omy19 in rainbow trout: Pleiotropy versus linkage. Plant and Animal Genome Conference. 361.
Detection of QTL in rainbow trout affecting survival when challenged with Flavobacterium psychrophilum - (Peer Reviewed Journal)
Vallejo, R.L., Palti, Y., Evenhuis, J., Gao, G., Liu, S., Rexroad III, C.E., Wiens, G.D. 2014. Detection of QTL in rainbow trout affecting survival when challenged with Flavobacterium psychrophilum. Marine Biotechnology. 16(3) 349-360. DOI 10.1007/s10126-013-9553-9.
Microevolution of Renibacterium salmoninarum: evidence for intercontinental dissemination associated with fish movements - (Peer Reviewed Journal)
Brynildsrud, O., Feil, E.J., Bohlin, J., Castillo-Ramirez, S., Colquhoun, D., Mccarthy, U., Matejusova, I.M., Rhodes, L., Wiens, G.D., Verner-Jeffreys, D.W. 2013. Microevolution of Renibacterium salmoninarum: evidence for intercontinental dissemination associated with fish movements. The ISME Journal: Multidisciplinary Journal of Microbial Ecology. 8: 746-756. DOI: 10.1038/ismej.2013.186.
Vaccination against salmonid bacterial kidney disease - (Book / Chapter)
Elliott, D.G., Wiens, G.D., Hammell, K., Rhodes, L.D. 2014. Vaccination against salmonid bacterial kidney disease. In: Gudding, R., Lillehaug, A., Evensen, O., editors. Fish Vaccination. 1st edition. Hoboken, NJ: WIley & Sons, Ltd. p. 255-272.
Assessment of genetic correlation between bacterial cold water disease resistance and spleen index in a domesticated population of rainbow trout: Identification of QTL on chromosome Omy19 - (Peer Reviewed Journal)
Wiens, G.D., Vallejo, R.L., Leeds, T.D., Palti, Y., Hadidi, S., Liu, S., Evenhuis, J., Welch, T.J., Rexroad Iii, C.E. 2013. Assessment of genetic correlation between bacterial cold water disease resistance and spleen index in a domesticated population of rainbow trout: Identification of QTL on chromosome Omy19. PLoS One. 8(10): e75749: DOI: 10.1371/journal.pone.0075749.
Acute mortality, bacterial load and pathology of select lines of adult rainbow trout challenged with Weissella sp. NC36 - (Peer Reviewed Journal)
Marancik, D.P., Welch, T.J., Leeds, T.D., Wiens, G.D. 2013. Acute mortality, bacterial load and pathology of select lines of adult rainbow trout challenged with Weissella sp. NC36. Journal of Aquatic Animal Health. 25(4): 230-236.
Comparison of disease resistance between diploid, induced-triploid, and intercross-triploid rainbow trout including trout selected for resistance to Flavobacterium psychrophilum - (Peer Reviewed Journal)
Weber, G.M., Wiens, G.D., Welch, T.J., Hostuttler, M.A., Leeds, T.D. 2013. Comparison of disease resistance between diploid, induced-triploid, and intercross-triploid rainbow trout including trout selected for resistance to Flavobacterium psychrophilum. Aquaculture. 410-411, 66-71.
Aquacultured rainbow trout (Oncorhynchus mykiss) possess a large core intestinal microbiota that is resistant to variation in diet and rearing density - (Peer Reviewed Journal)
Wong, S., Waldrop, T., Summerfelt, S., Davidson, J., Barrows, F., Kenney, P., Welch, T.J., Wiens, G.D., Snekvik, K., Rawls, J.F., Good, C. 2013. Aquacultured rainbow trout (Oncorhynchus mykiss) possess a large core intestinal microbiota that is resistant to variation in diet and rearing density. Applied and Environmental Microbiology. DOI:10.1128/AEM.00924-13.
Examination of resistance and tolerance in rainbow trout bred for differential susceptibility to bacterial cold water disease - (Abstract Only)
Marancik, D.P., Leeds, T.D., Blazer, V.S., Wiens, G.D. 2013. Examination of resistance and tolerance in rainbow trout bred for differential susceptibility to bacterial cold water disease. Annual Eastern Fish Health Workshop. 056.
On-farm performance of rainbow trout (Oncorhynchus mykiss) selectively bred for resistance to bacterial cold water disease: effect of rearing environment on survival phenotype Reprint Icon - (Peer Reviewed Journal)
Wiens, G.D., Lapatra, S.E., Welch, T.J., Evenhuis, J., Rexroad III, C.E., Leeds, T.D. 2013. On-farm performance of rainbow trout (Oncorhynchus mykiss) selectively bred for resistance to bacterial cold water disease: effect of rearing environment on survival phenotype. Aquaculture. 388-391:128-136.
Mapping QTL for spleen size and BCWD resistance in rainbow trout - (Abstract Only)
Vallejo, R.L., Palti, Y., Liu, S., Welch, T.J., Leeds, T.D., Evenhuis, J., Rexroad III, C.E., Wiens, G.D. 2013. Mapping QTL for spleen size and BCWD resistance in rainbow trout. Annual International Plant & Animal Genome Conference. P0508.
A real-time polymerase chain reaction assay for identification and quantification of Flavobacterium psychrophilum and application to disease resistance studies in selectively bred rainbow trout - (Peer Reviewed Journal)
Marancik, D.P., Wiens, G.D. 2012. A real-time polymerase chain reaction assay for identification and quantification of Flavobacterium psychrophilum and application to disease resistance studies in selectively bred rainbow trout. FEMS Microbiology Letters. doi: 10.1111/1574-6968.12061.
Spleen size is an indirect indicator of rainbow trout bacterial cold water disease resistance - (Abstract Only)
Wiens, G.D., Dharmasri, P., Stephens, L., Zwollo, P., Kaattari, S.L. 2013. Spleen size is an indirect indicator of rainbow trout bacterial cold water disease resistance. Aquaculture Conference Proceedings. P0214.
The pathophysiology of bacterial cold water disease in selectively-bred rainbow trout - (Abstract Only)
Marancik, D.P., Leeds, T.D., Wiens, G.D. 2013. The pathophysiology of bacterial cold water disease in selectively-bred rainbow trout. Aquaculture Conference Proceedings. P0154.
Field evaluation of rainbow trout selectively bred for resistance to bacterial cold water disease - (Proceedings)
Wiens, G.D., Leeds, T.D., Lapatra, S.E., Wilson, C., Cavendar, W., Rexroad III, C.E. 2012. Field evaluation of rainbow trout selectively bred for resistance to bacterial cold water disease. Flavobacterium Meeting. CD-ROM.
Mapping of QTL for bacterial cold water disease resistance in rainbow trout - (Abstract Only)
Palti, Y., Vallejo, R.L., Welch, T.J., Evenhuis, J., Leeds, T.D., Gao, G., Liu, S., Rexroad III, C.E., Wiens, G.D. 2013. Mapping of QTL for bacterial cold water disease resistance in rainbow trout. Aquaculture Conference Proceedings. P0296.
Flavobacterium Columnare studies in rainbow trout (Oncorhynchus mykiss) - (Abstract Only)
Evenhuis, J., Wiens, G.D., Lapatra, S.E. 2013. Flavobacterium Columnare studies in rainbow trout (Oncorhynchus mykiss). Aquaculture Conference Proceedings. P0353.
First identification of regulatory B cell subsets expressing IL-10 in teleost fish - (Abstract Only)
Takizawa, F., Gomez, D., Xu, Z., Parra, D., Bjork, S., Zhang, Y., Bartholomew, J., Wiens, G.D., Sunyer, J. 2012. First identification of regulatory B cell subsets expressing IL-10 in teleost fish. Congress International Society Develop Comparative Immunology. 367.
Field evaluation of rainbow trout selectively bred for resistance to bacterial cold water disease - (Abstract Only)
Wiens, G.D., Leeds, T.D., La Patra, S.E., Wilson, C., Cavendar, W., Rexroad III, C.E. 2012. Field evaluation of rainbow trout selectively bred for resistance to bacterial cold water disease. Flavobacterium Meeting. 126.
Mapping of QTL for bacterial cold water disease resistance in rainbow trout - (Abstract Only)
Palti, Y., Vallejo, R.L., Welch, T.J., Leeds, T.D., Evenhuis, J., Gao, G., Liu, S., Rexroad III, C.E., Wiens, G.D. 2012. Mapping of QTL for bacterial cold water disease resistance in rainbow trout [abstract]. International Symposium on Genetics in Aquaculture. p. 354.
Mapping of QTL for bacterial cold water disease resistance in rainbow trout - (Abstract Only)
Palti, Y., Vallejo, R.L., Welch, T.J., Leeds, T.D., Evenhuis, J., Liu, S., Rexroad III, C.E., Wiens, G.D. 2012. Mapping of QTL for bacterial cold water disease resistance in rainbow trout. Plant and Animal Genome Conference. P0662.
Detection of quantitative trait loci (QTL) for resistance to bacterial cold water disease and spleen size in rainbow trout - (Abstract Only)
Palti, Y., Vallejo, R.L., Welch, T.J., Evenhuis, J., Leeds, T.D., Liu, S., Rexroad III, C.E., Wiens, G.D. 2011. Detection of quantitative trait loci (QTL) for resistance to bacterial cold water disease and spleen size in rainbow trout. Aquaculture America Conference. 4.
Identification of immunogenic proteins within distinct molecular mass fractions of Flavobacterium psychrophilum Reprint Icon - (Peer Reviewed Journal)
Lafrentz, B.R., Lapatra, S.E., Call, D.R., Wiens, G.D., Cain, K.D. 2011. Identification of immunogenic proteins within distinct molecular mass fractions of Flavobacterium psychrophilum. Journal of Fish Diseases. 34:823-830.
Whole genome sequencing: the future for molecular epidemiological studies on aquatic pathogens - (Abstract Only)
Verner-Jeffreys, D., Baker-Austin, C., Amaro, C., Colquoun, D., Febrer, M., Feil, E.J., Gulig, P.A., Mccarthy, U., Rhodes, L., Wiens, G.D. 2011. Whole genome sequencing: the future for molecular epidemiological studies on aquatic pathogens [abstract]. European Association of Fish Pathologists. p. 105.
Origin and evolution of TNF and TNF receptor superfamilies - (Review Article)
Wiens, G.D., Glenney, G.W. 2011. Origin and evolution of TNF and TNF receptor superfamilies. Developmental and Comparative Immunology. DOI: 10.1016/j.dci.2011.03.031.
Tetraploid production in salmonids - (Abstract Only)
Weber, G.M., Hostuttler, M.A., Wiens, G.D., Beers, B., Leeds, T.D. 2011. Tetraploid production in salmonids. Aquaculture America Conference. 392.
Preliminary field evaluation of rainbow trout selectively bred for increased resistance to bacterial cold water disease - (Abstract Only)
Wiens, G.D., Leeds, T.D., Lapatra, S., Wilson, C., Cavendar, W., Evenhuis, J., Welch, T.J., Silverstein, J., Rexroad III, C.E. 2011. Preliminary field evaluation of rainbow trout selectively bred for increased resistance to bacterial cold water disease. Aquaculture America Conference. 308.
Detection of QTL for spleen size and disease resistance in rainbow trout - (Abstract Only)
Palti, Y., Vallejo, R.L., Welch, T.J., Leeds, T.D., Evenhuis, J., Liu, S., Rexroad III, C.E., Wiens, G.D. 2011. Detection of QTL for spleen size and disease resistance in rainbow trout. Plant and Animal Genome Conference. p. 643.
Evidence of major genes affecting resistance to bacterial cold water disease in rainbow trout using Bayesian methods of segregation analysis - (Peer Reviewed Journal)
Vallejo, R.L., Wiens, G.D., Rexroad III, C.E., Welch, T.J., Evenhuis, J., Leeds, T.D., Janss, L.L., Palti, Y. 2010. Evidence of major genes affecting resistance to bacterial cold water disease in rainbow trout using Bayesian methods of segregation analysis. Journal of Animal Science. 88:3814-3832.
Validating selective breeding approaches for disease resistance - (Trade Journal)
Leeds, T.D., Rexroad Iii, C.E., Wiens, G.D. 2010. Validating selective breeding approaches for disease resistance. Trout Talk. 2010:6.
Temporal and pathogen-load dependent changes in rainbow trout (Oncorhynchus mykiss) immune response traits following challenge with biotype 2 Yersinia ruckeri - (Peer Reviewed Journal)
Wiens, G.D., Vallejo, R.L. 2010. Temporal and pathogen-load dependent changes in rainbow trout (Oncorhynchus mykiss) immune response traits following challenge with biotype 2 Yersinia ruckeri. Fish and Shellfish Immunology. doi:10.1016/j.fsi.2010.06.010 29:639-647.
Applying Selective Breeding And Vaccination To Improve Fish Health - (Abstract Only)
Wiens, G.D. 2010. Applying Selective Breeding And Vaccination To Improve Fish Health. Annual Eastern Fish Health Workshop. p.23-24
Identification, characterization and genetic mapping of the TLR1 gene in rainbow trout (Oncorhynchus mykiss) - (Peer Reviewed Journal)
Palti, Y., Rodriguez, M., Gahr, S.A., Purcell, M., Rexroad III, C.E., Wiens, G.D. 2010. Identification, characterization and genetic mapping of the TLR1 gene in rainbow trout (Oncorhynchus mykiss). Fish and Shellfish Immunology. 28:918-926.
Response to selection for bacterial cold water disease resistance in rainbow trout - (Peer Reviewed Journal)
Leeds, T.D., Silverstein, J., Weber, G.M., Vallejo, R.L., Palti, Y., Rexroad III, C.E., Evenhuis, J., Hadidi, S., Welch, T.J., Wiens, G.D. 0210. Response to selection for bacterial cold water disease resistance in rainbow trout. Journal of Animal Science. 88:1936-1946.
Bacterial kidney disease (Renibacterium salmoninarum) - (Book / Chapter)
Wiens, G.D. 2011. Bacterial kidney disease (Renibacterium salmoninarum). Fish Diseases and Disorders. 3:338-374.
Inheritance of rainbow trout Oncorhynchus mykiss spleen size and correlation with bacterial cold water disease resistance - (Abstract Only)
Wiens, G.D., Vallejo, R.L., Leeds, T.D., Palti, Y., Evenhuis, J., Welch, T.J., Rexroad Iii, C.E. 2010. Inheritance of rainbow trout Oncorhynchus mykiss spleen size and correlation with bacterial cold water disease resistance. World Aquaculture Society Meeting 2010. Paper No. 87.
Evidence of major genes affecting bacterial cold water disease resistance in rainbow trout using Bayesian methods of complex segregation analysis - (Abstract Only)
Vallejo, R.L., Wiens, G.D., Rexroad Iii, C.E., Welch, T.J., Evenhuis, J., Leeds, T.D., Janss, L.L., Palti, Y. 2010. Evidence of major genes affecting bacterial cold water disease resistance in rainbow trout using Bayesian methods of complex segregation analysis. Plant and Animal Genome Conference XVIII. Paper No. 103.
Resistance of rainbow trout to Flavobacterium psychrophilum infection: integrating selective breeding and microbial genomics - (Proceedings)
Wiens, G.D., Leeds, T.D., Vallejo, R.L., Palti, Y., Welch, T.J., Rexroad III, C.E. 2009. Resistance of rainbow trout to Flavobacterium psychrophilum infection: integrating selective breeding and microbial genomics. Flavobacterium Meeting.
Identification, characterization, and genetic mapping of TLR7, TLR8a1 and TLR8a2 genes in rainbow trout (Oncorhynchus mykiss) - (Peer Reviewed Journal)
Palti, Y., Gahr, S.A., Purcell, M.K., Hadidi, S., Rexroad III, C.E., Wiens, G.D. 2010. Identification, characterization, and genetic mapping of TLR7, TLR8a1 and TLR8a2 genes in rainbow trout (Oncorhynchus mykiss). Developmental and Comparative Immunology. 34:219-233.
Proteomic analysis of Flavobacterium psychrophilum cultured in vivo and in iron-limited media Reprint Icon - (Peer Reviewed Journal)
Lafrentz, B.R., Lapatra, S.E., Call, D.R., Wiens, G.D., Cain, K.D. 2009. Proteomic analysis of Flavobacterium psychrophilum cultured in vivo and in iron-limited media. Diseases of Aquatic Organisms. 87:171-182.
Response to selection for bacterial cold water disease resistance in rainbow trout - (Abstract Only)
Leeds, T.D., Silverstein, J., Vallejo, R.L., Palti, Y., Rexroad III, C.E., Evenhuis, J., Hadidi, S., Weber, G.M., Welch, T.J., Wiens, G.D. 2009. Response to selection for bacterial cold water disease resistance in rainbow trout. International Symposium on Genetics in Aquaculture.
Resistance of rainbow trout to Flavobacterium psychrophilum infection: integrating selective breeding and microbial genomics - (Abstract Only)
Wiens, G.D., Leeds, T.D., Vallejo, R.L., Palti, Y., Welch, T.J., Rexroad Iii, C.E. 2009. Resistance of rainbow trout to Flavobacterium psychrophilum infection: integrating selective breeding and microbial genomics. Flavobacterium 2009 Meeting. Paper No. 100.
Variable regions in Flavobacterium psychrophilum strains identified by comparative genomics: application to selective breeding for cold water disease resistance - (Abstract Only)
Wiens, G.D., Lapatra, S.E., Palti, Y., Vallejo, R.L., Leeds, T.D., Welch, T.J., Rexroad III, C.E. 2009. Variable regions in Flavobacterium psychrophilum strains identified by comparative genomics: application to selective breeding for cold water disease resistance. American Fishery Society (Fish Health Section) Proceedings.
Response to selection for bacterial cold water disease resistance in rainbow trout - (Abstract Only)
Leeds, T.D., Silverstein, J., Vallejo, R.L., Palti, Y., Rexroad III, C.E., Evenhuis, J., Hadidi, S., Weber, G.M., Welch, T.J., Wiens, G.D. 2009. Response to selection for bacterial cold water disease resistance in rainbow trout. American Fishery Society (Fish Health Section) Proceedings.
Comparative genomics of the Flavobacterium psychrophilum Frp locus - (Abstract Only)
Wiens, G.D., Lapatra, S.E., Call, D.R., Welch, T.J. 2009. Comparative genomics of the Flavobacterium psychrophilum Frp locus. American Society for Microbiology Meeting.
Proteomic analysis of Flavobacterium psychrophilum cultured in vivo and in iron-limited media - (Proceedings)
Lafrentz, B.R., Lapatra, S.E., Call, D.R., Wiens, G.D., Cain, K.D. 2009. Proteomic analysis of Flavobacterium psychrophilum cultured in vivo and in iron-limited media. In: Proceeding of the Members of the Genus Flavobacterium. Flavobacterium 2009, September 21-23, 2009, Paris, France. CDROM.
Microbial Genomics of Aquaculture Pathogens - (Book / Chapter)
Wiens, G.D. 2009. Microbial Genomics of Aquaculture Pathogens. In: Overturf, K., editor. Current Status of Molecular Techniques in Aquaculture. Hobolen, NJ: Wiley, John & Sons, Inc. p. 315-335.
Evidence of major genes for resistance to bacterial cold-water disease in rainbow trout using mixed inheritance multiple-threshold models and Bayesian segregation analysis - (Abstract Only)
Vallejo, R.L., Wiens, G.D., Welch, T.J., Rexroad Iii, C.E., Palti, Y. 2009. Evidence of major genes for resistance to bacterial cold-water disease in rainbow trout using mixed inheritance multiple-threshold models and Bayesian segregation analysis. Gordon Research Conference Proceedings.
Rainbow trout resistance to bacterial cold-water disease is moderately heritable and is not adversely correlated with growth Reprint Icon - (Peer Reviewed Journal)
Silverstein, J., Vallejo, R.L., Palti, Y., Welch, T.J., Wiens, G.D., Rexroad Iii, C.E., Ducrocq, V., Leeds, T.D. 2008. Rainbow trout resistance to bacterial cold-water disease is moderately heritable and is not adversely correlated with growth. Journal of Animal Science. 87:860-867.
Renibacterium salmoninarum p57 antigenic variation is restricted in geographic distribution and correlated with genomic markers - (Peer Reviewed Journal)
Wiens, G.D., Dale, O. 2009. Renibacterium salmoninarum p57 antigenic variation is restricted in geographic distribution and correlated with genomic markers. Diseases of Aquatic Organisms. 83:123-131.
Genome sequence of the fish pathogen Renibacterium salmoninarum suggests reductive evolution away from an environmental Arthrobacter ancestor. - (Peer Reviewed Journal)
Wiens, G.D., Rockey, D.D., Wu, Z., Crane, S., Chen, D.S., Capri, G.R., Burnett, J.R., Ponnerassary, S., Schimpma, M.J., Burd, H., Bhattacharyya, A., Rhodes, L.D., Strom, M.S. 2008. The genome sequence of Renibacterium salmoninarum suggests reductive evolution away from an environmental Arthrobacter ancestor. Journal of Bacteriology. 190(21):6970-6982.
Integrating selective breeding with microbial genomics to improve rainbow trout disease resistance - (Abstract Only)
Wiens, G.D. 2008. Integrating selective breeding with microbial genomics to improve rainbow trout disease resistance. Meeting Abstract. p. 104.
Challenges associated with heterologous expression of Flavobacterium psychrophilum proteins in Escherichia coli - (Peer Reviewed Journal)
Shah, D.H., Cain, K.D., Wiens, G.D., Call, D.R. 2008. Challenges associated with heterologous expression of Flavobacterium psychrophilum proteins in Escherichia coli. Marine Biotechnology. 10(6):719-730. Epub 2008 Jun 13.
Suggestive association of major histocompatibility IB genetic markers with resistance to bacterial cold water disease in rainbow trout (Oncorhynchus mykiss) - (Peer Reviewed Journal)
Johnson, N., Vallejo, R.L., Silverstein, J., Welch, T.J., Wiens, G.D., Hallerman, E.M., Palti, Y. 2008. Suggestive association of major histocompatibility IB genetic markers with resistance to bacterial cold water disease in rainbow trout (Oncorhynchus mykiss). Marine Biotechnology. 10:429-437.
Spleen Size Predicts Resistance of Rainbow Trout to Flavobacterium psychrophilum Challenge - (Peer Reviewed Journal)
Hadidi, S., Glenney, G., Welch, T.J., Silverstein, J., Wiens, G.D. 2008. Spleen Size Predicts Resistance of Rainbow Trout to Flavobacterium psychrophilum Challenge. Journal of Immunology. 180:4156-4165.
Progress Toward An Immunogenetic Map for Rainbow Trout - (Abstract Only)
Palti, Y., Vallejo, R.L., Wiens, G.D., Rexroad III, C.E. 2008. Progress toward an immunogenetic map for rainbow trout. Plant and Animal Genome Conference.
Recent Advances in Bacterial Coldwater Disease Research - (Trade Journal)
Wiens, G.D., Welch, T.J. 2007. Recent Advances in Bacterial Coldwater Disease Research. Trout Talk Pg 5-6.
Progress Toward An Immunogenetic Map for Rainbow Trout - (Abstract Only)
Palti, Y., Rexroad III, C.E., Vallejo, R.L., Wiens, G.D. 2007. Progress toward an immunogenetic map for rainbow trout. Meeting Abstract.
Genome Sequence of Flavobacterium psychrophilum Strain CSF 259-93 and Characterization of a Large Cluster of Genes Containing Short Repeats - (Abstract Only)
Wiens, G.D., Welch, T.J., Rexroad III, C.E., Lapatra, S., Call, D.R., Hunnicutt, D., Bhattacharyya, A., Campbell, J., Walunas, T. 2007. Genome Sequence of Flavobacterium psychrophilum Strain CSF 259-93 and Characterization of a Large Cluster of Genes Containing Short Repeats. Meeting Abstract Pg 12.
Rainbow Trout Innate Immunity against Flavobacterium psychrophilum - (Abstract Only)
Hadidi, S., Glenney, G.W., Welch, T.J., Silverstein, J., Blazer, V., Wiens, G.D. 2007. Rainbow Trout Innate Immunity against Flavobacterium psychrophilum. Meeting Abstract Pg 35.
Progress Toward An Immunogenetic Map for Rainbow Trout - (Abstract Only)
Fincham, M.R., Wiens, G.D., Rexroad III, C.E., Vallejo, R.L., Palti, Y. 2007. Progress toward an immunogenetic map for rainbow trout. Meeting Abstract.
Associations of Markers Linked to Major Histocompatibility (MH) Regions With Disease Resistance in Rainbow Trout - (Abstract Only)
Palti, Y., Johnson, N., Rexroad III, C.E., Welch, T.J., Wiens, G.D., Silverstein, J., Vallejo, R.L. Associations of Markers Linked to Major Histocompatibility (MH) Regions With Disease Resistance in Rainbow Trout. Meeting Abstract.
Early Diversification of the Tumor Necrosis Superfamily in Teleosts: Genomic Characterization and Expression Analysis - (Peer Reviewed Journal)
Glenney, G.W., Wiens, G.D. 2007. Early Diversification of the Tumor Necrosis Superfamily in Teleosts: Genomic Characterization and Expression Analysis. Journal of Immunology, 178:7955-7973.
Spleen Size Predicts Innate Resistance of Rainbow Trout to Flavobacterium psychrophilum Challenge - (Abstract Only)
Hadidi, S., Glenney, G., Welch, T.J., Silverstein, J., Wiens, G.D. 2007. Spleen Size Predicts Innate Resistance of Rainbow Trout to Flavobacterium psychrophilum Challenge. American Society for Microbiology.
Distribution of Yersinia pestis pIP1202-like Multidrug Resistance Plasmids Among Foodborne Pathogens - (Abstract Only)
Welch, T.J., Fricke, W.F., Eppinger, M., Rosovitz, M.J., Mcdermott, P., White, D., Wiens, G.D., Ravel, J. 2007. Distribution of Yersinia pestis pIP1202-like Multidrug Resistance Plasmids Among Foodborne Pathogens. American Society of Microbiologists Abstracts.
Identification of potential vaccine target antigens by immunoproteomic analysis of a virulent and a non-virulent strain of the fish pathogen Flavobacterium psychrophilum - (Peer Reviewed Journal)
Sudheesh, P.S., Lafrentz, B.R., Call, D.R., Siems, W.F., Lapatra, S.E., Wiens, G.D., Cain, K.D. 2007. Identification of potential vaccine target antigens by immunoproteomic analysis of a virulent and a non-virulent strain of the fish pathogen Flavobacterium psychrophilum. Diseases of Aquatic Organisms. 74(1):37-47.
Associations of Markers Linked to Major Histocompatibility (MH) Regions and to Other Immune Response Genes with Disease Resistance in Rainbow Trout - (Abstract Only)
Palti, Y., Johnson, N., Rexroad III, C.E., Welch, T.J., Wiens, G.D., Silverstein, J., Vallejo, R.L. 2006. Associations of Markers Linked to Major Histocompatibility (MH) Regions and to Other Immune Response Genes with Disease Resistance in Rainbow Trout. Plant and Animal Genome Abstracts Pg. 11.
Associations of Markers Linked to Major Histocompatibility (MH) Regions and to Other Immune Response Genes with Disease Resistance in Rainbow Trout - (Abstract Only)
Palti, Y., Johnson, N., Rexroad III, C.E., Welch, T.J., Wiens, G.D., Silverstein, J., Vallejo, R.L. 2006. Associations of Markers Linked to Major Histocompatibility (MH) Regions and to Other Immune Response Genes with Disease Resistance in Rainbow Trout. World Aquaculture Society Meeting.
Cracking the Code: Selecting for Resistance Against Bacterial Cold-Water Disease - (Trade Journal)
Silverstein, J., Palti, Y., Vallejo, R.L., Welch, T.J., Wiens, G.D. 2006. Cracking the code: selecting for resistance against bacterial cold-water disease. Trout Talk.
Application of Microbial Genomics to Improve Aquatic Animal Health - (Abstract Only)
Wiens, G.D. 2006. Application of Microbial Genomics to Improve Aquatic Animal Health. Meeting Abstract.
Selective Breeding and Genetic Mapping of Disease Resistance in Rainbow Trout - (Abstract Only)
Palti, Y., Rexroad Iii, C.E., Welch, T.J., Wiens, G.D., Silverstein, J., Vallejo, R.L. 2006. Selective breeding and genetic mapping of disease resistance in rainbow trout. Meeting Abstract. Presented at The 9th International Symposium of The International Association for Genetics in Aquaculture, June 26-30, 2006 at Montpellier, France. Meeting Book of Abstracts p. 81.
Gene Expression and Cellular Immune Response of Rainbow Trout (Oncorhynchus Mykiss) Exposed to Yersinia Ruckeri: a Model System for Understanding Mechanisms of Teleost Protective Immunity - (Abstract Only)
Wiens, G.D., Lapatra, S., Palti, Y., Rexroad III, C.E., Yao, J., Welch, T.J. 2006. Gene expression and cellular immune response of rainbow trout (Oncorhynchus mykiss) exposed to Yersinia ruckeri: A model system for understanding mechanisms of teleost protective immunity. 10th International Congress, International Society of Developmental and Comparative Immunology, July 1 - 6, 2006. Meeting Abstract. Page 249.
Early Diversification of the Tumor Necrosis Superfamily in Teleosts: Genomic Characterization and Expression Analysis - (Abstract Only)
Glenney, G., Wiens, G.D. 2006. Early diversification of the tumor necrosis superfamily in teleosts: genomic characterization and expression analysis. 10th International Congress, International Society of Developmental and Comparative Immunology. Meeting Abstract Page 113.
Bacterial Kidney Disease - (Book / Chapter)
Wiens, G.D. 2006. Bacterial kidney disease. Aquaculture Compendium. Online at www.cabicompendium.org/ac. Wallingford, UK: CAB International
Identification of Novel Rainbow Trout (Onchorynchus Mykiss) Chemokines, Cxcd1 and Cxcd2: Mrna Expression Following Yersinia Ruckeri Vaccination and Challenge - (Peer Reviewed Journal)
Wiens, G.D., Glenney, G.W., Lapatra, S.E., Welch, T.J. 2006. Identification of novel rainbow trout (Onchorynchus mykiss) chemokines, cxcd1 and cxcd2: Mrna expression following Yersinia ruckeri vaccination and challenge. Immunogenetics. 2006 May;58(4):308-23. Epub 2006 Mar 29.
Characterization of Major Histocompatibility (Mh) Haplotypes and Their Association with Disease Resistance in Rainbow Trout - (Abstract Only)
Palti, Y., Rexroad III, C.E., Welch, T.J., Wiens, G.D., Silverstein, J. 2005. Characterization of major histocompatibility (MH) haplotypes and their association with desease resistance in rainbow trout. Plant and Animal Genome Conference. Abstract ID P643, page 262.
Antigenic Variation in Renibacterium Salmoninarum P57: Functional and Diagnostic Implications - (Abstract Only)
Wiens, G.D., Ole Bendik, D. 2005. Antigenic variation in Renibacterium salmoninarum p57: functional and diagnostic implications. Bacterial Kidney Disease: Challenges for the 21st Century. Meeting Abstract. November 15-17, 05 Seattle, WA p. 21.
Novel Vaccine Targets and Assessment of Cellular Immunity - (Abstract Only)
Wiens, G.D. 2005. Novel vaccine targets and assessment of cellular immunity.Workshop on genomics of Renibacterium saloninarum. Meeting Abstract Bacterial Kidney Disease: Challenges for the 21st Century. p. 12.
Construction of a Virulent, Green Fluorescent Protein-Tagged Yersinia Ruckeri and Detection in Trout Tissues after Intraperitoneal and Immersion Challenge - (Peer Reviewed Journal)
Welch, T.J., Wiens, G.D. 2005. Construction of a virulent, green fluorescent protein-tagged yersinia ruckeri and detection in trout tissues after intraperitoneal and immersion challenge. Diseases of Aquatic Organisms. 67:267-272.
Identification and Expression of An Immuno-Reactive Heat Shock Protein from Flavobacterium Psychrophilum - (Abstract Only)
Cain, K., Sudheesh, P.S., Lapatra, S.E., Wiens, G.D., Lafrentz, B.R., Call, D.R. 2005. Identification and expression of an immuno-reactive heat shock protein from flavobacterium psychrophilum. Annual Meeting of the Fish Health Section/American Fisheries Society. (abstract).
Characterization of Toll-Like Receptor 3 Gene in Rainbow Trout (Oncorhynchus Mykiss) - (Peer Reviewed Journal)
Rodriguez, M.F., Wiens, G.D., Purcell, M., Palti, Y. 2005. Characterization of toll-like receptor 3 gene in rainbow trout (oncorhynchus mykiss). Immunogenetics 57(7):510-519. Online pub date 2005 Aug 6, :1-10.
Genomic Characterization of Rainbow Trout (Oncorhynchus Mykiss) Toll-Like Receptor Genes - (Abstract Only)
Rodriguez, M.F., Wiens, G.D., Gahr, S.A., Rexroad III, C.E., Palti, Y. 2005. Genomic characterization of rainbow trout (Oncorhynchus mykiss) toll-like receptor genes. Plant and Animal Genome XIII Abstract W030 page 12.
Mapping Neutralizing Epitopes on Renibacterium Salmoninarum P57 Using Transposon Mutagenesis and Synthetic Peptides - (Peer Reviewed Journal)
Wiens, G.D., Owens, J. 2005. Mapping neutralizing epitopes on renibacterium salmoninarum p57 using transposon mutagenesis and synthetic peptides. Applied and Environmental Microbiology 71 (6): 2894-2901.
Genome Sequencing of the Vertically-Transmitted Fish Pathogen Renibacterium Salmoninarum - (Abstract Only)
Strom, M., Wiens, G.D., Rockey, D. 2004. Genome sequencing of the vertically-transmitted fish pathogen renibacterium salmoninarum. Meeting Abstract P 90-93.
Estimation of the Genome Size of Renibacterium Salmoninarum Atcc 33209 by Pulsed-Field Gel Electrophoresis - (Abstract Only)
Wiens, G.D., Strom, M.S. 2005. Estimation of the genome size of renibacterium salmoniarum atcc 33209 by pulsed-tield gel electrophoresis. (Abstract). 30th Eastern Fish Health Workshop. p. 8.
Genomic Characterization and Expression Analysis of a Tumor Necrosis Factor Superfamily 13b (Tnfsf 13b) Homologue from Rainbow Trout (Oncorhynchus Mykiss) - (Abstract Only)
Wiens, G.D., Gahr, S.A., Morrison, C., Palti, Y., Rexroad III, C.E., Rodriguez, M.F., Welch, T.J. 2004. Genomic characterization and expression analysis of a tumor necrosis factor superfamily 13b (tnfsf 13b) homologue from rainbow trout (oncorhynchus mykiss). International Immunology Congress p. 82C (abstract W15.15).
Identification of Tumor Necrosis Factor (Tnf) and Tnf-Receptor Superfamily Members Expressed in Rainbow Trout (Oncorhynchus Mykiss) - (Abstract Only)
Wiens, G.D. 2004. Identification of tumor necrosis factor (tnf) and tnf-receptor superfamily members expressed in rainbow trout (oncorhynchus mykiss). Annual Eastern Fish Health Workshop p 52.
A Proposal Advocating Draft Sequencing the Genome of Rainbow Trout, Oncorhynchus Mykiss - (Review Article)
Rexroad III, C.E., Palti, Y., Thorgaard, G., Hansen, J., Silverstein, J., Weber, G.M., Wiens, G.D., Gahr, S.A., Rodriguez, M.F., Coulibaly, I., Overturf, K. 2004. A proposal advocating draft sequencing the genome of rainbow trout, oncorhynchus mykiss. Government Publication/Report.. Dept of Energy Joint Genome Institute Community Sequencing Program.
Molecular Characterization of a Putative Tumor Necrosis Factor Superfamily 13b (Tnfsf 13b) Homologue from Rainbow Trout (Oncorhynchus Mykiss) - (Abstract Only)
Wiens, G.D., Palti, Y., Rexroad III, C.E., Welch, T.J. 2003. Molecular characterization of a putative tumor necrosis factor superfamily 13b (tnfsf 13b) homologue from rainbow trout (oncorhynchus mykiss). Immunology Research Workshop. p.35.
Characterization and Mapping of Immune Response Genes in Rainbow Trout (Oncorhynchus Mykiss) - (Abstract Only)
Rodriguez, M.F., Hansen, J., Wiens, G.D., Welch, T.J., Palti, Y. 2003. Characterization and mapping of immune response genes in rainbow trout (oncorhynchus mykiss). Meeting Abstract. Nov 9-15, 2003. p.4.
Mapping Antibody Epitopes on Renibacterium Salmoninarum P57 Using Transposon Mutagenesis and Synthethic Peptides - (Abstract Only)
Wiens, G.D, Owen, J., Pascho, R., Winton, J.R. 2003. Mapping antibody epitopes on renibacterium salmoninarum p57 using transposon mutagenesis and synthethic peptides. 28th Annual Eastern Fish Health Workshop. p.70.
Repertoire Shift in the Humoral Response to Phosphocholine-Keyhole Limpet Hemocyanin: Vh Somatic Mutation in Germinal Center B Cells Impairs T15 Ig Function - (Peer Reviewed Journal)
Wiens, G.D., Brown, M., Rittenberg, M.B. 2003. Repertoire shift in the humoral response to phosphocholine-keyhole limpet hemocyanin: vh somatic mutation in germinal center b cells impairs t15 ig function.. Journal of Immunology. 170:5095-5102.
Last Modified: 4/17/2014
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