Page Banner

United States Department of Agriculture

Agricultural Research Service

David Matthew. Donovan

Research Molecular Biologist


Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Potential of the virion-associated peptidoglycan hydrolase HydH5 and its derivative fusion proteins in milk biopreservation - (Peer Reviewed Journal)
Rodriguez-Rubio, L., Martinez, B., Donovan, D.M., Garcia, P., Rodriguez, A. 2013. Potential of the virion-associated peptidoglycan hydrolase HydH5 and its derivative fusion proteins in milk biopreservation. Applied Microbiology and Biotechnology. 8(1):e54828.
Bacteriophage endolysins expressed in yeast kill strains of Lactobacillus that contaminate fermentations - (Abstract Only) - (15-Aug-13)
Bacteriophage endolysin production in Nicotiana benthamiana plants - (Abstract Only) - (24-Jun-13)
Expression of bacteriophage endolysins in Saccharomyces cerevisiae - (Abstract Only) - (21-May-13)
Alternatives to antibiotics: a symposium on the challenges and solutions for animal production - (Peer Reviewed Journal)
Seal, B.S., Gay, C.G., Lillehoj, H.S., Donovan, D.M. 2013. Alternatives to antibiotics: a symposium on the challenges and solutions for animal production. Animal Health Research Reviews. 14(1):78-87.
Physicochemical characterization of Staphylococcus aureus-lysing LysK enzyme in complexes with polycationic - (Peer Reviewed Journal)
Filatova, L.Y., Donovan, D.M., Becker, S.C., Lebedev, D.N., Priyma, A.D., Koudriachova, E.V., Kabanov, A.V., Klyachko, N.L. 2013. Physicochemical characterization of Staphylococcus aureus-lysing LysK enzyme in complexes with polycationic. Biochimie.
Undetectable bacterial resistance to phage lytic proteins from the Staphylococcus aureus bacteriophage vB_SauS-phiIPLA88 - (Peer Reviewed Journal)
Rodriguez-Rubio, L., Martinez, B., Rodriguez, A., Donovan, D.M., Götz, F., García, P. 2013. Undetectable bacterial resistance to phage lytic proteins from the Staphylococcus aureus bacteriophage vB_SauS-phiIPLA88. PLoS One. 8(5):e64671.
Chimeric Ply187 endolysin kills Staphylococcus aureus more effectively than the parental enzyme. - (Peer Reviewed Journal)
Mao, J., Donovan, D.M. 2013. Chimeric Ply187 endolysin kills Staphylococcus aureus more effectively than the parental enzyme. Applied and Environmental Microbiology. 342(1):30-36.
Bacteriophage-encoded lytic enzymes control growth of contaminating Lactobacillus found in fuel ethanol fermentations - (Peer Reviewed Journal)
Roach, D.R., Khatibi, P.A., Bischoff, K.M., Hughes, S.R., Donovan, D.M. 2013. Bacteriophage-encoded lytic enzymes control growth of contaminating Lactobacillus found in fuel ethanol fermentations. Biotechnology for Biofuels. 6(1):20.
Virus of microbes - (Peer Reviewed Journal)
Ceyssens, P., Aertsen, A., Donovan, D.M., Lavigne, R., Sullivan, M., Debarbieux, L., Vaneechoutte, M. 2012. Virus of microbes. Microbiological Research. (12)00196-9.
Bacteriophage endolysins as novel antimicrobials - (Review Article)
Schmelcher, M., Donovan, D.M., Loessner, M. 2012. Bacteriophage endolysins as novel antimicrobials. Future Microbiology.
Bacteriophage virion-associated peptidoglycan hydrolases: potential new enzybiotics - (Review Article)
Rodriguez-Rubio, L., Martinez, B., Donovan, D.M., Rodriguez, A., Garcia, P. 2013. Bacteriophage virion-associated peptidoglycan hydrolases: potential new enzybiotics. Critical Reviews in Microbiology.
Staphylococcal phage 2638a endolysin is lytic for Staphylococcus aureus and harbors an inter-lytic-domain cryptic translational start site. - (Peer Reviewed Journal)
Abaev, I., Foster Frey, J.A., Korobova, O., Shishkova, N., Kopylov, P., Pyramchuk, S., Schmelcher, M., Becker, S.C., Donovan, D.M. 2013. Staphylococcal phage 2638a endolysin is lytic for Staphylococcus aureus and harbors an inter-lytic-domain cryptic translational start site. Applied and Environmental Microbiology. 97(8):3449-3456.
Chimeric phage lysins act synergistically with lysostaphin to kill mastitis causing staphylococcus aureus in murine mammary glands - (Peer Reviewed Journal)
Schmelcher, M., Powell, A.M., Becker, S.C., Camp, M.J., Donovan, D.M. 2012. Chimeric phage lysins act synergistically with lysostaphin to kill mastitis causing staphylococcus aureus in murine mammary glands. Applied and Environmental Microbiology. 78(7):2297-305.
Enhanced staphylolytic activity of the Staphylococcus aureus bacteriophage vB_SauS-phiIPLA88 HydH5 virion associated peptidoglycan hydrolase: fusions, deletions and synergy with LysH5 - (Peer Reviewed Journal)
Rodriguez, L., Martinez, B., Rodriguez, A., Donovan, D.M., Garcia, P. 2012. Enhanced staphylolytic activity of the Staphylococcus aureus bacteriophage vB_SauS-phiIPLA88 HydH5 virion associated peptidoglycan hydrolase: fusions, deletions and synergy with LysH5. Antimicrobial Agents and Chemotherapy. 78(7):2241-8.
Endolysins as antimicrobials - (Book / Chapter)
Nelson, D.C., Schmelcher, M., Rodirguez, L., Klumpp, J., Pritchard, D.G., Dong, S., Donovan, D.M. 2012. Endolysins as antimicrobials. Advances in Virus Research.
Lytic activity of the virion-associated peptidoglycan hydrolase HydH5 of staphylococcus aureus bacteriophage vB_SauS-phiIPLA88 - (Peer Reviewed Journal)
Rodriguez, L., Martinez, B., Zhou, Y., Rodriguez, A., Donovan, D.M., Garcia, P. 2011. Lytic activity of the virion-associated peptidoglycan hydrolase HydH5 of staphylococcus aureus bacteriophage vB_SauS-phiIPLA88. BioMed Central Biology. 11:138-145.
Lysostaphin: molecular changes that preserve staphylolytic activity. - (Proceedings) - (20-Feb-11)
Dopamine transporter-dependent and -independent striatal binding of the benztropine analog JHW 007, a cocaine antagonist with low abuse liability - (Peer Reviewed Journal)
Kopajtic, T.A., Liu, Y., Surratt, C.K., Donovan, D.M., Newman, A.H., Katz, J.L. 2010. Dopamine transporter-dependent and -independent striatal binding of the benztropine analog JHW 007, a cocaine antagonist with low abuse liability. Journal of Pharmacology and Experimental Therapeutics. 335(3):703-714.
Staphylococcus aureus - (Book / Chapter)
Weese, S.J., Mao, J., Donovan, D.M. 2010. Staphylococcus aureus. In: Fratamico, P., Liu, Y., Kathariou, S., editors. Genomes of Foodborne and Waterborne Pathogens. 1st edition. Washington, DC: American Society for Microbiology. p. 113-123.
LysK, the enzyme lysing Staphylococcus aureus cells: specific kinetic features and approaches towards stabilization - (Review Article)
Filatova, L.Y., Becker, S.C., Donovan, D.M., Gladilin, A.K., Klyachko, N.L. 2010. LysK, the enzyme lysing Staphylococcus aureus cells: specific kinetic features and approaches towards stabilization. Biochimie. 92(5):507-13.
Peptidoglycan Hydrolases for Control of Mastitis Pathogens - (Abstract Only)
Schmelcher, M., Becker, S.C., Foster Frey, J.A., Donovan, D.M. 2009. Peptidoglycan Hydrolases for Control of Mastitis Pathogens. Evergreen International Phage Meeting.
Engineering Antimicrobials Refractory to Resistance - (Abstract Only)
Becker, S.C., Pohl, C.S., Mohammadi, H., Schmelcher, M., Foster Frey, J.A., Lease, R.A., Don, S., Baker, J.R., Pritchard, D.G., Donovan, D.M. 2009. Engineering Antimicrobials Refractory to Resistance. Evergreen International Phage Meeting.
Peptidoglycan hydrolase fusion to protein transduction domains kill intracellular staphylococci. - (Abstract Only)
Becker, S.C., Foster Frey, J.A., Willard, R.R., Lease, R.A., Almeida, R., Marriott, I., Dong, S., Baker, J.R., Pritchard, D.G., Donovan, D.M. 2009. Peptidoglycan hydrolase fusion to protein transduction domains kill intracellular staphylococci. Evergreen International Phage Meeting.
Differentially conserved staphylococcal SH3b_5 cell wall binding domains confer increased staphylolytic and streptolytic activity to a streptococcal prophage endolysin domain. - (Peer Reviewed Journal)
Becker, S.C., Foster-Frey, J., Stodola, A.J., Anacker, D., Donovan, D.M., 2009. Differentially conserved staphylococcal SH3b_5 cell wall binding domains confer identical staphylolytic activity to a streptococcal prophage endolysin lytic domain. FEMS Immunology and Medical Microbiology. 443(1-2):32-41.
Antimicrobials for staphylococcal pathogens that are refractory to resistance development - (Abstract Only)
Becker, S.C., Foster Frey, J.A., Willard, R.R., Lease, R.A., Almeida, R., Marriott, I., Dong, S., Pritchard, D.G., Donovan, D.M. 2009. Antimicrobials for staphylococcal pathogens that are refractory to resistance development. International Symposium on Antimicrobial Peptides.
Engineering of Peptidoglycan Hydrolases for Control of Pathogenic Bacteria - (Abstract Only)
Schmelcher, M., Loessner, M.J., Donovan, D.M. 2009. ENGINEERING OF PEPTIDOGLYCAN HYDROLASES FOR CONTROL OF PATHOGENIC BACTERIA. BARC Poster Day.
Antimicrobials for mastitis causing pathogens that are refractory to resistance development - (Abstract Only)
Becker, S.C., Don, S., Baker, J.R., Foster Frey, J.A., Pritchard, D.G., Donovan, D.M. 2009. Antimicrobials for mastitis causing pathogens that are refractory to resistance development. BARC Poster Day.
Comparison of two bacteriophage derived enzymes with lytic activity against strains of Clostridium perfringens - (Abstract Only)
Simmons, I.M., Siragusa, G.R., Donovan, D.M., Seal, B.S. 2009. Comparison of two bacteriophage derived enzymes with lytic activity against strains of Clostridium perfringens. American Society for Microbiology.
Expression of Two Bacteriophage Enzymes that Lyse Clostridium perfringens which Share Sequences in the Cell Binding Domain of the Molecules but are Dissimilar in their Catalytic Enzymatic Domain - (Abstract Only)
Simmons, I.M., Siragusa, G., Donovan, D.M., Seal, B.S. 2009. Expression of Two Bacteriophage Enzymes that Lyse Clostridium perfringens which Share Sequences in the Cell Binding Domain of the Molecules but are Dissimilar in their Catalytic Enzymatic Domain. International Symposium on Antimicrobial Peptides.
Peptidoglycan hydrolase enzyme fusions are uniquely suited for treating multi-drug resistant pathogens - (Review Article)
Donovan, D.M., Becker, S.C., Dong, S., Baker, J.R., Foster Frey, J.A., Pritchard, D.G. 2009. Peptidoglycan hydrolase enzyme fusions are uniquely suited for treating multi-drug resistant pathogens. Biotech International 21(2):6-10.
LysK CHAP endopeptidase domain is required for lysis of live staphylococcal cells. - (Peer Reviewed Journal)
Becker, S.C., Don, S., Baker, J.R., Foster Frey, J.A., Pritchard, D.G., Donovan, D.M. 2009. LysK CHAP endopeptidase domain is required for lysis of live staphylococcal cells. FEMS Microbiology Letter. 294(1):52-60.
Significant modulation of mitochondrial electron transport system by nicotine in various rat brain regions - (Peer Reviewed Journal)
Wang, J., Kim, J., Donovan, D.M., Becker, K.G., Li, M.D. 2009. Significant modulation of mitochondrial electron transport system by nicotine in various rat brain regions. Mitochondrion. 9:186-195.
Genome walk of an unknown upstream region of myostatin gene in Spanish goats - (Abstract Only)
Singh, M., Amoah, E., Kannan, G., Stice, S., Donovan, D.M. 2009. Genome walk of an unknown upstream region of myostatin gene in Spanish goats. Reproductive Biology.
A Novel Use of a Bacteriophage Lysin, PlyC, as a Disinfectant against Streptococcus equi - (Peer Reviewed Journal)
Hoopes, J.T., Stark, C.J., Kim, H.A., Sussman, D.J., Donovan, D.M., Nelson, D.C. 2009. A Novel Use of a Bacteriophage Lysin, PlyC, as a Disinfectant against Streptococcus equi. Applied and Environmental Microbiology. 75(5):1388-94.
Identification of Known Phage Endolysin Genes by Pcr - (Abstract Only) - (05-Aug-08)
SH3b Cell wall binding domains can enhance anti-staphylococcal activity of endolysin lytic domains. - (Abstract Only) - (10-Jul-08)
Engineering Antimicrobials that are Refractory to Resistance Development. - (Abstract Only)
Donovan, D.M., Becker, S.C. 2009. Engineering Antimicrobials that are Refractory to Resistance Development. Meeting Abstract.
Engineering MRSA antimicrobials that are refractory to resistance development - (Abstract Only)
Becker, S.C., Donovan, D.M., Foster Frey, J.A. 2008. Engineering MRSA antimicrobials that are refractory to resistance development. BARC Poster Day.
Use of a bacteriophage lysin for barnyard decontamination of Streptococcus equi - (Abstract Only)
Hoopes, J.T., Donovan, D.M., Sussman, D.J., Trayer, T.P., Stark, C.J., Nelson, D.C. 2008. Use of a bacteriophage lysin for barnyard decontamination of Streptococcus equi. American Society for Microbiology.
Genotyping Staphylococcus aureus allows one to identify bacteriophages harboring unknow endolysins. Reprint Icon - (Abstract Only)
Donovan, D.M., Abaev, I., Schischkova, N., Myakinina, V., Korobova, O., Pecherskich, E., Kopylov, P., Kiseleva, N. 2008. Genotyping Staphylococcus aureus allows one to identify bacteriophages harboring unknow endolysins. Meeting Abstract.
Chronic nicotine exposure regulates the mitochondrial electron transport system in multiple brain regions of rat - (Abstract Only)
Wang, J., Kim, J., Donovan, D.M., Becker, K.G., Li, M. 2007. Chronic nicotine exposure regulates the mitochondrial electron transport system in multiple brain regions of rat. Society for Neuroscience Abstracts and Proceedings.
Resolving the database sequence discrepancies for the Staphylococcus aureus bacteriophage phi 11 amidase. Reprint Icon - (Peer Reviewed Journal)
Donovan D.M., Foster-Frey J., Garrett W.M., Blomberg, L. 2008. Resolving the database sequence discrepancies for the Staphylococcus aureus bacteriophage phi11 amidase. Journal of Basic Microbiology. 48(1):48-52.
Assessment of the in vitro binding of JHW 007, a dopamine transport inhibitor that blocks the effects of cocaine - (Abstract Only)
Kopajtic, T.A., Donovan, D.M., Newman, A.H., Katz, J.L. 2007. Assessment of the in vitro binding of JHW 007, a dopamine transport inhibitor that blocks the effects of cocaine. Society for Neuroscience Abstracts and Proceedings.
A summary of staphylococcal C-terminal SH3b_5 cell wall binding domains. - (Abstract Only)
Donovan, D.M., Stodola, A.J., Annacker, D. 2007. A summary of staphylococcal C-terminal SH3b_5 cell wall binding domains.. Evergreen International Phage Meeting.
Bacteriophage and peptidoglycan degrading enzymes with antimicrobial applications. - (Review Article)
Donovan, D.M. 2007. Bacteriophage and peptidoglycan degrading enzymes with antimicrobial applications. Review Article. Recent Patents on Biotechnology. 1(2):113-122.
Designer Antimicrobials: Peptidoglycan Hydrolase Module Shuffling - (Abstract Only) - (30-Oct-06)
Bacteriophage Phi11 Endolysin Has Antimicrobial Activity Against the Mastitis Pathogens Staphylococcus Aureus and Coagulase Negative Staphylococcus - (Peer Reviewed Journal)
Donovan, D.M., Foster Frey, J.A. 2006. Bacteriophage phi11 endolysin has antimicrobial activity against the mastitis pathogens staphylococcus aureus and coagulase negative staphylococcus. Applied and Environmental Microbiology. 265(1):133-139.
The Cell Lysis Activity of the Streptococcus Agalactiae Bacteriophage B30 Endolysin Relies on the Chap Endopeptidase Domain. - (Peer Reviewed Journal)
Donovan, D.M., Foster Frey, J.A., Dong, S., Rousseau, G., Moineau, S., Pritchard, D.G. 2006. The cell lysis activity of the streptococcus agalactiae bacteriophage b30 endolysin relies on the chap endopeptidase domain. Applied Environmental Microbiology. 72:5108-5112.
Designer Antimicrobials: Peptidoglycan Hydrolase Module Shuffling - (Abstract Only)
Donovan, D.M., Dong, S., Garrett, W.M., Foster Frey, J.A., Rousseau, G.M., Moineau, S., Pritchard, D.G. 2006. Designer Antimicrobials: Peptidoglycan Hydrolase Module Shuffling. American Society for Microbiology.
Engineering Disease Resistant Cattle - (Review Article)
Donovan, D.M., Kerr, D.E., Wall, R.J. 2005. Engineering disease resistant cattle. Transgenic Research. 14(5):563-567.
Follicular Expression of a Human Beta-Cell Leukemia/lymphoma-2 (Bcl-2)transgene Does Not Decrease Atresia Or Increase Ovulation Rate in Swine - (Peer Reviewed Journal)
Guthrie, H.D., Wall, R.J., Pursel, V.G., Foster Frey, J.A., Donovan, D.M., Dawson, H.D., Welch, G.R., Garrett, W.M. 2005. Follicular expression of a human B-cell leukemia/lymphoma-2 (Bcl-2)transgene does not decrease atresia or increase ovulation rate in swine. Journal Of Reproduction, Fertility And Development. 17(4):457-468.
Follicular Expression of a Human Beta-Cell Leukemia/lymphoma-2 (Bcl-2)transgene Does Not Decrease Atresia Or Increase Ovulation Rate in Swine - (Abstract Only)
Guthrie, H.D., Wall, R.J., Pursel, V.G., Donovan, D.M., Welch, G.R., Garrett, W.M. 2004. Follicular expression of a human beta-cell leukemia/lymphoma-2 (bcl-2)transgene does not decrease atresia or increase ovulation rate in swine [abstract]. Society for the Study of Reproduction Annual Meeting. p. 275.
Expression of a Group B Streptococcus Bacteriophage Lysin in Mammalian Cells. - (Abstract Only)
Donovan, D.M., Wall, R.J., Pritchard, D., Talbot, N.C. 2004. Expression of a group b streptococcus bacteriophage lysin in mammalian cells. [abstract]. American Society for Microbiology. No. M-035.
Identification and Characterization of Metallothionein-1 and 2 Gene Expression in the Context of (±)3,4-Methylenedioxymethamphetamine (Mdma)-Induced Toxicity to Brain Dopaminergic Neurons. - (Abstract Only)
Xie, T., Tong, L., Mccann, U., Yuan, J., Becker, K., Mechan, A., Cheadle, C., Donovan, D.M., Ricaurte, G. 2004. Identification and characterization of metallothionein-1 and 2 gene expression in the context of (±)3,4-methylenedioxymethamphetamine (mdma)-induced toxicity to brain dopaminergic neurons. Society for Neuroscience Abstracts and Proceedings. 24(32):7043-7050.
Identification and Annotation of 11,363 Clones from a Murine Dopamine Neuron-Specific Cdna Library Using Sequencing and Various Bioinformatics Approaches - (Abstract Only)
Li, M.D., Zhang, D., Taylor, W., Donovan, D.M., Becker, K.G. 2004. Identification and annotation of 11,363 clones from a murine dopamine neuron-specific cdna library using sequencing and various bioinformatics approaches. [abstract]. Society for Neuroscience Abstracts and Proceedings. 809.10.
Identification and Characterization of Metallothionein-1 and 2 Gene Expression in the Context of (±)3,4-Methylenedioxymethamphetamine (Mdma)-Induced Toxicity to Brain Dopaminergic Neurons - (Peer Reviewed Journal)
Xie, T., Tong, L., Mccann, U., Yuan, J., Becker, K., Mechan, A., Cheadle, C., Donovan, D.M., Ricaurte, A. 2004. Identification and characterization of metallothionein-1 and 2 gene expression in the context of (±)3,4-methylenedioxymethamphetamine (mdma)-induced toxicity to brain dopaminergic neurons. Journal of Neuroscience. 24(32):7043-7050.
Follicular Expression of a Human Beta-Cell Leukemia/lymphoma-2 (Bcl-2)transgene Does Not Decrease Atresia Or Increase Ovulation Rate in Swine. - (Abstract Only)
Guthrie, H.D., Wall, R.J., Pursel, V.G., Foster Frey, J.A., Donovan, D.M., Welch, G.R., Garrett, W.M. 2004. Follicular expression of a human beta-cell leukemia/lymphoma-2 (bcl-2)transgene does not decrease atresia or increase ovulation rate in swine. Society for the Study of Reproduction Annual Meeting. Abstract. p275.
Microsoft Excel Spread-Sheet Based Animal Colony Management for Genetically Altered Mouse Strains. - (Peer Reviewed Journal)
Donovan, D.M., Rowley, D.L., Williams, N.G. 2004. Microsoft excel spread-sheet based animal colony management for genetically altered mouse strains. Biotechniques. 37(1):50-56.
Role of U-Opioid Receptors in Formalin-Induced Pain Behavior in Mice - (Peer Reviewed Journal)
Zhao, C., Tao, Y., Tall, J.M., Donovan, D.M., Meyer, R.A., Raja, S.N. Role of u-opioid receptors in formalin-induced pain behavior in mice. Journal of Experimental Neurology. 2003. 184(2):839-45.
Microarray Analysis Reveals Interleukin-6 As a Novel Secretory Product of the Hypothalamo-Neurohypophyseal. - (Peer Reviewed Journal)
Ghorbel, M.T., Sharman, G., Leroux, M., Barrett, T., Donovan, D.M., Becker, K.G., Murphy, D. Microarray analysis reveals interleukin-6 as a novel secretory product of the hypothalamo-neurohypophyseal.. Journal of Biological Chemistry.2003 MAY 23; 278(21):19280-5. EPUB 2003 MARCH 14
Last Modified: 9/26/2012
Footer Content Back to Top of Page