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Lev Nemchinov
Research Molecular Biologist


Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Complete genome sequence of alfalfa-associated picorna-like virus 2 - (Peer Reviewed Journal)
Nemchinov, L.G., Grinstead, S.C. 2024. Complete genome sequence of alfalfa-associated picorna-like virus 2. Microbiology Resource Announcements. 13. Article e01052-23. https://doi.org/10.1128/mra.01052-23.
Alfalfa vein mottling virus, a novel potyvirid infecting Medicago sativa L - (Peer Reviewed Journal)
Nemchinov, L.G., Postnikova, O.A., Wintermantel, W.M., Palumbo, J.C., Grinstead, S.C. 2023. Alfalfa vein mottling virus, a novel potyvirid infecting Medicago sativa L. Virology Journal. 20:284. https://doi.org/10.1186/s12985-023-02250-5.
Composition of the alfalfa pathobiome in commercial fields - (Peer Reviewed Journal)
Nemchinov, L.G., Irish, B.M., Uschapovsky, I.V., Grinstead, S.C., Shao, J.Y., Postnikova, O.A. 2023. Composition of the alfalfa pathobiome in commercial fields. Frontiers in Microbiology. 14: Article e1225781. https://doi.org/10.3389/fmicb.2023.1225781.
Characterization of the seed virome of alfalfa (Medicago sativa L) - (Peer Reviewed Journal)
Nemchinov, L.G., Irish, B.M., Grinstead, S.C., Postnikova, O.A. 2023. Characterization of the seed virome of alfalfa (Medicago sativa L). Virology Journal. 20. Article 96. https://doi.org/10.1186/s12985-023-02063-6.
Snake River alfalfa virus, a persistent virus infecting alfalfa (Medicago sativa L.) in Washington State, USA - (Peer Reviewed Journal)
Postnikova, O.A., Irish, B.M., Eisenback, J., Nemchinov, L.G. 2023. Snake River alfalfa virus, a persistent virus infecting alfalfa (Medicago sativa L.) in Washington State, USA. Virology Journal. 20. Article 32. https://doi.org/10.1186/s12985-023-01991-7.
Diversity of the virome associated with alfalfa (Medicago sativa L.) in the U.S. Pacific Northwest - (Peer Reviewed Journal)
Nemchinov, L.G., Irish, B.M., Grinstead, S.C., Shao, J.Y., Vieira, P. 2022. Diversity of the virome associated with alfalfa (Medicago sativa L.) in the U.S. Pacific Northwest. Scientific Reports. 12:8726. https://doi.org/10.1038/s41598-022-12802-4.
Expanding the RNA virome of nematodes and other soil-inhabiting organisms - (Peer Reviewed Journal)
Vieira, P., Subbotin, S.A., Alkharouf, N., Eisenback, J.D., Nemchinov, L.G. 2022. Advancing virus discovery in nematodes and other soil-inhabiting organisms. Virus Evolution. 8(1):1–8. https://doi.org/10.1093/ve/veac019.
Targeted transcriptomics reveals signatures of large-scale independent origins and concerted regulation of effector genes in Radopholus similis - (Peer Reviewed Journal)
Viera, P., Myers, R.Y., Pellegrin, C., Wram, C., Hesse, C.N., Maier, T.R., Georgios, K.D., Zasada, I.A., Matsumoto Brower, T.K., Danchin, E.G., Baum, T.J., Eves-Van Den Akker, S., Nemchinov, L.G. 2021. Targeted transcriptomics reveals signatures of large-scale independent origins and concerted regulation of effector genes in Radopholus similis. PLoS Pathogens. 17:11. https://doi.org/10.1371/journal.ppat.1010036.
Genome-wide identification of endogenous viral sequences in alfalfa (Medicago sativa L.) - (Peer Reviewed Journal)
Transcription factors in alfalfa (Medicago sativa L.): genome-wide identification and a web resource center AlfalfaTFDB - ()
Nemchinov L.G., Shao J., Grinstead S., Postnikova O.A. (2021) Transcription Factors in Alfalfa (Medicago sativa L.): Genome-Wide Identification and a Web Resource Center AlfalfaTFDB. In: Yu LX., Kole C. (eds) The Alfalfa Genome. Compendium of Plant Genomes. Springer, Cham. https://doi.org/10.1007/978-3-030-74466-3_7
The root lesion nematode effector Ppen10370 is essential for parasitism of Pratylenchus penetrans - (Peer Reviewed Journal)
Vieira, P., Vicente, C.S., Branco, J., Bauchan, G.R., Mota, M., Nemchinov, L.G. 2021. The root lesion nematode effector Ppen10370 is essential for parasitism of Pratylenchus penetrans. Molecular Plant-Microbe Interactions. https://doi.org/10.1094/MPMI-09-20-0267-R.
High-throughput sequencing for deciphering the virome of alfalfa (Medicago sativa L.) - (Peer Reviewed Journal)
Bejerman, N., Roumagnac, P., Nemchinov, L.G. 2020. High-throughput sequencing for deciphering the virome of alfalfa (Medicago sativa L.). Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2020.553109.
Identification of complete genome sequencing of alfalfa virus S diagnosed in alfalfa plants (Medicago sativa L.) from Washington State, USA - (Peer Reviewed Journal)
Nemchinov, L.G., Grinstead, S.C., Irish, B.M., Shao, J.Y. 2020. Identification of complete genome sequencing of alfalfa virus S diagnosed in alfalfa plants (Medicago sativa L.) from Washington State, USA. Plant Disease. https://doi.org/10.1094/PDIS-06-20-1374-PDN.
Identification of a novel isolate of Alfalfa virus S from China suggests a possible role of seed contamination in the distribution of the virus - (Peer Reviewed Journal)
Nemchinov, L.G., Mollov, D.S., Grinstead, S.C. 2020. Identification of a novel isolate of Alfalfa virus S from China suggests a possible role of seed contamination in the distribution of the virus. Plant Disease. https://doi.org/10.1094/PDIS-04-20-0906-SC.
Prevalence of the root lesion nematode virus (RLNV1) in populations of Pratylenchus penetrans from North America - (Peer Reviewed Journal)
Vieira, P., Peetz, A.B., Mimee, B., Saikai, K., Mollov, D.S., Macguidwin, A., Zasada, I.A., Nemchinov, L.G. 2020. Prevalence of the root lesion nematode virus (RLNV1) in populations of Pratylenchus penetrans from North America. Journal of Nematology. 52:e2020-45. https://doi.org/10.21307/jofnem-2020-045.
Identification of emerging viral genomes in transcriptomic datasets of alfalfa (Medicago sativa L.) - (Peer Reviewed Journal)
Jiang, P., Shao, J.Y., Nemchinov, L.G. 2019. Identification of emerging viral genomes in transcriptomic datasets of alfalfa (Medicago sativa L.). Virology Journal. 16:153. https://doi.org/10.1186/s12985-019-1257-y.
An expansin-like candidate effector protein from Pratylenchus penetrans modulates immune responses in Nicotiana benthamiana - (Peer Reviewed Journal)
Vieira, P., Nemchinov, L.G. 2019. An expansin-like candidate effector protein from Pratylenchus penetrans modulates immune responses in Nicotiana benthamiana. Phytopathology. https://doi.org/10.1094/PHYTO-09-19-0336-R.
Genome size dynamics within multiple genera of diploid seed plants - (Peer Reviewed Journal)
Boutanaev, A.M., Nemchinov, L.G. 2020. Genome size dynamics within multiple genera of diploid seed plants. Russian Journal of Genetics: Applied Research. 56/667-676. https://doi.org/10.1134/S1022795420060046.
Identification of the coding-complete genome of Cycas necrotic stunt virus in transcriptomic data sets of alfalfa (Medicago sativa) - (Peer Reviewed Journal)
Jiang, P., Shao, J.Y., Nemchinov, L.G. 2019. Identification of the coding-complete genome of Cycas necrotic stunt virus in transcriptomic data sets of alfalfa (Medicago sativa). Microbiology Resource Announcements. 8:e00981-19. https://doi.org/10.1128/MRA.00981-19.
Cellular and trancriptional responses of resistant and susceptible cultivars of alfalfa to the root lesion nematode, Pratylenhus penetrans - (Peer Reviewed Journal)
Vieira, P., Mowery, J.D., Eisenback, J.D., Shao, J.Y., Nemchinov, L.G. 2019. Cellular and trancriptional responses of resistant and susceptible cultivars of alfalfa to the root lesion nematode, Pratylenhus penetrans. Frontiers in Plant Science. 10:971. https://doi.org/10.3389/fpls.2019.00971.
A novel species of RNA virus associated with root lesion nematode Pratylenchus penetrans - (Peer Reviewed Journal)
Vieira, P., Nemchinov, L.G. 2019. A novel species of RNA virus associated with root lesion nematode Pratylenchus penetrans. Journal of General Virology. https://doi.org/10.1099/jgv.0.001246#tab2.
Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans - (Peer Reviewed Journal)
Vicente, C.S., Nemchinov, L.G., Mota, M., Eisenback, J.D., Kamo, K.K., Vieira, P. 2019. Identification and characterization of the first pectin methylesterase gene discovered in the root lesion nematode Pratylenchus penetrans. PLoS One. 14 (2):e0212540. https://doi.org/10.1371/journal.pone.0212540.
First report of alphapartitiviruses infecting alfalfa (Medicago sativa) in the United States - (Peer Reviewed Journal)
Nemchinov, L.G., Lee, M., Shao, J.Y. 2018. First report of alphapartitiviruses infecting alfalfa (Medicago sativa) in the United States. Genome Announcements. https://doi.org/mra.asm.org/content/7/21/e01266-18.
Characterization of alfalfa virus F, a new member of the genus Marafivirus - (Peer Reviewed Journal)
Nemchinov, L.G., Francois, S., Roumagnac, P., Hammond, R., Mollov, D.S., Filloux, D. 2018. Characterization of alfalfa virus F, a new member of the genus Marafivirus. PLoS One. 13(9):e0203477. https://doi.org/journals.plos.org/plosone/article?id=10.1371/journal.pone.0203477.
Resistant and susceptible responses in alfalfa (Medicago sativa) to bacterial stem blight caused by Pseudomonas syringae pv. syringae - (Peer Reviewed Journal)
Nemchinov, L.G., Shao, J.Y., Lee, M., Postnikova, O.A., Samac, D.A. 2017. Resistant and susceptible responses in alfalfa (Medicago sativa) to bacterial stem blight caused by Pseudomonas syringae pv. syringae. PLoS One. 12(12):e0189781.
A first report and complete genome sequence of alfalfa enamovirus from Sudan - (Peer Reviewed Journal)
Nemchinov, L.G., Grinstead, S.C., Mollov, D.S. 2017. A first report and complete genome sequence of alfalfa enamovirus from Sudan. Genome Announcements. e00531-17. doi: 10.1128/genomeA.00531-17.
Alfalfa virus S, a new species in the family Alphaflexiviridae - (Peer Reviewed Journal)
Nemchinov, L.G., Grinstead, S.C., Mollov, D.S. 2017. Alfalfa virus S, a new species in the family Alhapflexiviridae. PLoS One. 12(5):e0178222.
Development and characterization of the first infectious clone of alfalfa latent virus, a strain of Pea streak virus - (Peer Reviewed Journal)
Nemchinov, L.G. 2017. Development and characterization of the first infectious clone of alfalfa latent virus, a strain of Pea streak virus. European Journal of Plant Pathology. doi:10.1007/s10658-017-1237-2.
Complete nucleotide sequence and genome organization of a novel allexivirus from alfalfa (Medicago sativa) - (Abstract Only)
Recombinant sheep pox virus proteins elicit neutralizing antibodies - (Peer Reviewed Journal)
Chervyakova, O.V., Zaitsev, V.L., Iskakov, B.K., Tailakova, E.T., Strochkov, V.M., Sultankulova, K.T., Sandybayev, N.T., Stanbekova, G.E., Beisenov, D.K., Abduraimov, Y.O., Mambetaliyev, M., Sansyzbay, A., Kovalskaya, N.Y., Nemchinov, L.G., Hammond, R. 2016. Recombinant sheep pox virus proteins elicit neutralizing antibodies. Viruses. 8(6)159.
Virus-induced gene silencing of RPC5-like subunit of RNA polymerase III caused pleiotropic effects in Nicotiana benthamiana - (Peer Reviewed Journal)
Nemchinov, L.G., Boutanaev, A.M., Postnikova, O.A. 2016. Virus-induced gene silencing of RPC5-like subunit of RNA polymerase III caused pleiotropic effects in Nicotiana benthamiana. Scientific Reports. 6:27785.
Gene expression profiling in viable but not culturable (VBNC) cells of Pseudomonas syringae pv syringae - (Peer Reviewed Journal)
Postnikova, O., Shao, J.Y., Mock, N.M., Baker, C.J., Nemchinov, L.G. 2015. Gene expression profiling in viable but not culturable (VBNC) cells of Pseudomonas syringae pv syringae. Frontiers in Microbiology. 6:1419.
Complete genome sequence of the alfalfa latent virus - (Peer Reviewed Journal)
Nemchinov, L.G., Shao, J.Y., Postnikova, O.A. 2015. Complete genome sequence of the alfalfa latent virus. Genome Announcements. 3(2):e00250-15.
Natural antisense transcripts associated with salinity response in alfalfa - (Peer Reviewed Journal)
Postnikova, O., Nemchinov, L.G. 2015. Natural antisense transcripts associated with salinity response in alfalfa. The Plant Genome. 8:1-5.
Transcriptome analysis of resistant and susceptible alfalfa cultivars infected with root-knot nematode Meloidogyne incognita - (Peer Reviewed Journal)
Postnikova, O., Hult, M.N., Shao, J.Y., Skantar, A.M., Nemchinov, L.G. 2015. Transcriptome analysis of resistant and susceptible alfalfa cultivars infected with root-knot nematode Meloidogyne incognita. PLoS One. 10(3):e0123157.
Characterization of apoplast phenolics: Invitro oxidation of acetosyringone results in a rapid prolonged increase in the redox potential - (Peer Reviewed Journal)
Baker, C.J., Mock, N.M., Whitaker, B.D., Hammond, R., Roberts, D.P., Nemchinov, L.G., Aver'Yanov, A.A. 2014. Characterization of apoplast phenolics: Invitro oxidation of acetosyringone results in a rapid prolonged increase in the redox potential. Physiological and Molecular Plant Pathology. 86:57-63.
In silico identification of transcription factors in medicago sativa using available transcriptomic resources - (Peer Reviewed Journal)
Postnikova, O., Shao, J.Y., Nemchinov, L.G. 2014. In silico identification of transcription factors in medicago sativa using available transcriptomic resources. Molecular Genetics and Genomics. 289:457-468.
Analysis of alfalfa root transcriptome in response to salinity stress - (Peer Reviewed Journal)
Postnikova, O.A., Shao, J.Y., Nemchinov, L.G. 2013. Analysis of alfalfa root transcriptome in response to salinity stress. Plant And Cell Physiology. 54(7):1041-1055.
Comparative analysis of microarray data in Arabidopsis transcriptome during compatible interactions with plant viruses - (Peer Reviewed Journal)
Postnikova, O.A., Nemchinov, L.G. 2012. Comparative analysis of microarray data in Arabidopsis transcriptome during compatible interactions with plant viruses. Virology Journal. 9(101):1-7.
Endomembrane Ca2+ -ATPases play significant role in virus-induced adaptation to oxidative stress - (Peer Reviewed Journal)
Shabala, S.N., Baekgaard, L., Shabala, L., Fuglsang, A.T., Cuin, T.A., Nemchinov, L.G., Palmgren, M.G. 2011. Endomembrane Ca2+ -ATPases play significant role in virus-induced adaptation to oxidative stress. Plant Signaling and Behavior. 6(7):1053-1056.
Multiple transport systems mediate virus-induced acquired resistance to oxidative stress - (Peer Reviewed Journal)
Shabala, S., Baekgaard, L., Shabala, L., Fuglsang, A.T., Cuin, T.A., Palmgren, M.G., Nemchinov, L.G. 2011. Multiple transport systems mediate virus-induced acquired resistance to oxidative stress. Plant Cell and Environment. 34(3):406-411.
Non-invasive microelectrode potassium flux measurements as a potential tool for early recognition of virus-host compatibility in plants - (Peer Reviewed Journal)
Shabala, S.N., Nemchinov, L.G. 2010. Microelectrode non-invasive potassium flux measurements as a potential tool for early recognition of plant virus-host compatibility. Planta. 232(4):807-815.
Mapping of Heterologous Expressed Sequence Tags as an Alternative to Microarrays for Study of Defense Responses in Plants - (Peer Reviewed Journal)
Boutaneav, A.M., Postnikova, O.A., Nemchinov, L.G. 2009. Mapping of Heterologous Expressed Sequence Tags as an Alternative to Microarrays for Study of Defense Responses in Plants. Biomed Central (BMC) Genomics. 10:273.
Further improvement of PVX/CMV CP expression tool for presentation of short foreign epitopes - (Peer Reviewed Journal)
Natilla, A., Nemchinov, L.G. 2008. Further improvement of PVX/CMV CP expression tool for presentation of short foreign epitopes. Protein Expression and Purification. 59(1):117-121.
Calcium Efflux as a Component of Hypersensitive Response of Nicotiana benthamiana to Pseudomonas syringae - (Peer Reviewed Journal)
Nemchinov, L.G., Shabala, L., Shabala, S. 2008. Calcium Efflux as a Component of Hypersensitive Response of Nicotiana benthamiana to Pseudomonas syringae. Plant And Cell Physiology. 49(1):40-46.
Expression of animal CED-9 anti-apoptotic gene in tobacco modifies plasma membrane ion fluxes in response to salinity and oxidative stress - (Peer Reviewed Journal)
Shabala, S., Cuin, T.A., Prismall, L., Nemchinov, L.G. 2007. Expression of animal CED-9 anti-apoptotic gene in tobacco modifies plasma membrane ion fluxes in response to salinity and oxidative stress. Planta. 227(1):189-197.
Transient expression of the ectodomain of matrix protein 2 (M2e) of Avian Influenza A Virus in plants - (Peer Reviewed Journal)
Nemchinov, L.G., Natilla, A. 2007. Transient expression of the ectodomain of matrix protein 2 (M2e) of Avian Influenza A Virus in plants. Protein Expression and Purification. Available: doi:10.1016/j.pep.2007.05.015.
BOVINE CD14 RECEPTOR PRODUCED IN PLANTS REDUCES SEVERITY OF INTRAMAMMARY BACTERIAL INFECTION - (Peer Reviewed Journal)
Nemchinov, L.G., Paape, M.J., Sohn, E.J., Bannerman, D.D., Zarlenga, D.S., Hammond, R. 2006. Bovine cd14 receptor produced in plants reduces severity of intramammary bacterial infection.
EPITOPE PRESENTATION SYSTEM BASED ON CUCUMBER MOSAIC VIRUS COAT PROTEIN EXPRESSED FROM A POTATO VIRUS X-BASED VECTOR - (Peer Reviewed Journal)
Natilla, A., Hammond, R., Nemchinov, L.G. 2005.Epitope presentation system based on cucumber mosaic virus coat protein expressed from a potato virus x-based vector. Archives of Virology. 15:1373-1386.
DEVELOPMENT OF PLANT-DERIVED SUBUNIT VACCINE CANDIDATE AGAINST NEWCASTLE DISEASE VIRUS - (Abstract Only)
Natilla, A., Hammond, R., Zhao, Y., Nemchinov, L.G. 2005. Development of plant-derived subunit vaccine candidate against Newcastle disease virus. American Phytopathological Society Potomac Division Meeting. 95:S163.
DISPLAY OF VACCINE CANDIDATE ANTIGENS ON THE SURFACE OF CUCUMBER MOSAIC VIRUS COAT PROTEIN, EXPRESSED THROUGH A POTATO VIRUS X VECTOR - (Abstract Only)
Natilla, A., Hammond, R., Zhao, Y., Nemchinov, L.G. 2005. Display of vaccine candidate antigens on the surface of cucumber mosaic virus coat protein, expressed through a potato virus x vector. International Congress of Virology. p.163.
PLANT VIRUS-BASED EXPRESSION OF FUNCTIONAL CD14 LPS RECEPTOR IN PLANTS - (Abstract Only)
Nemchinov, L.G., Paape, M.J., Sohn, E.J., Bannerman, D.D., Zarlenga, D.S., Hammond, R. 2005. Plant virus-based expression of functional cd14 lps receptor in plants. American Society for Virology Meeting. p.85.
PLANT PRODUCTION OF VETERINARY VACCINES AND THERAPEUTICS - ()
Hammond, R., Nemchinov, L.G. 2009. Plant Production Of Veterinary Vaccines and Therapeutics. Current Topics in Microbiology and Immunology. 332:79-102.
MUTATION AND RECOMBINATION MAY LIMIT THE USE OF CUCUMBER MOSAIC VIRUS AS A VIRUS-BASED GENE EXPRESSION VECTOR - (Abstract Only)
Hammond, R., Nemchinov, L.G., Zhao, Y. 2004. Mutation and recombination may limit the use of cucumber mosaic virus as a virus-based gene expression vector. American Phytopathological Society. 94(S):912
EXAMINATION OF THE UTILITY OF PLANT VIRUS CAPSID PROTEIN FUSIONS TO PRODUCE NEWCASTLE DISEASE VIRUS VACCINE ANTIGENS - (Proceedings)
Nemchinov, L.G., Zhao, Y., Hammond, R. 2004. Examination of the utility of plant virus capsid protein fusions to produce newcastle disease virus vaccine antigens. Meeting Proceedings. p.64.
THE COMPLETE NUCLEOTIDE SEQUENCE, GENOME ORGANIZATION, AND SPECIFIC DETECTION OF BEET MOSAIC VIRUS - (Peer Reviewed Journal)
Nemchinov, L.G., Hammond, J., Jordan, R.L., Hammond, R. 2004. The complete nucleotide sequence, genome organization, and specific detection of beet mosaic virus. Archives of Virology. 149:1201-1204.
CLONING AND SEQUENCE ANALYSIS OF THE GENOME OF BEET MOSAIC POTYVIRUS - (Abstract Only)
PLANT VIRUS-BASED VECTORS IN AGRICULTURE AND BIOTECHNOLOGY - (Abstract Only)