Projects

DEVELOP ENHANCED PHYTASES FOR ANIMAL FEED AND FOR INCORPORATION INTO NEW PLANT CULTIVARS REQUIRING LESS PHOSPHORUS FERTILIZERS Appropriated (D)   Accession Number: 418203

Publications

Impact of Assay conditions on activity estimate and kinetics comparison of Aspergillus niger PhyA and Escherichia coli AppA2 phytases - (Peer Reviewed Journal)

Weaver, J.D., Ullah, A.H., Sethumadhavan, K., Mullaney, E.J., Lei, X.G. 2009. Comparisons of activity assay methods and kinetics of Aspergillus niger PhyA and Escherichia coli AppA2 phytases. Journal of Agriculture and Food Chemistry. 57:5315-5320.

Unfolding and Refolding of Aspergillus Niger PhyB Phytase: Role of Disulfide Bridges - (Peer Reviewed Journal)

Ullah, A.H., Sethumadhavan, K., Mullaney, E.J. 2008. Unfolding and Refolding of Aspergillus Niger PhyB Phytase: Role of Disulfide Bridges. Journal of Agriculture and Food Chemistry. 56(17):8179-8183.

Kinetic Characterization of O-Phospho-L-Tyrosine Phosphohydrolase Activity of Two Fungal Phytases. - (Peer Reviewed Journal)

Ullah, A.H., Sethumadhavan, K., Mullaney, E.J. 2008. Kinetic characterization of o-phospho-L-tyrosine phosphohydrolase activity of two fungal phytases. Journal of Agriculture and Food Chemistry. 56(16):7467-7471.

Salt Effect on the pH Profile and Kinetic Parameters of Microbial Phytases - (Peer Reviewed Journal)

Ullah, A.H., Sethumadhavan, K., Mullaney, E.J. 2008. Salt effect on the pH profile and kinetic parameters of microbial phytases. Journal of Agricultural and Food Chemistry. 56:3398-3402.

OPTIMIZING THE PH PROFILE OF FUNGAL PHYTASE THROUGH PROTEIN ENGINEERING ALSO ENHANCES ACTIVITY - (Abstract)

Mullaney, E.J., Ullah, A.H., Sethumadhavan, K., Lei2, X. 2007. Optimizing the ph profile of fungal phytase through protein engineering also enhances activity (abstract). Society of Industrial Microbiology Annual Meeting. p. 146.

Enzymatic Comparisons of Aspergillus niger PhyA and Escherichia coli AppA2 Phytases - (Abstract)

Weaver, J.D., Ullah, A.H., Sethumadhavan, K., Mullaney, E.J., Lei, X.G. 2007. Enzymatic comparisons of Aspergillus Niger PhyA and Escherichia Coli AppA2 Phytases (abstract). Journal of Animal Science. 85(Supplement 1):647.

New Uses for Immobilized Enzymes & Substrates on Cotton & Cellulose Fibers - (Book/Chapter)

Edwards, J.V., Ullah, A.H., Sethumadhavan, K., Batiste, S.L., Bel, P., Von Hoven, T.M., Goynes Jr, W.R., Condon, B.D., Pierre, S.C. 2007. New Uses for Immobilized Enzymes & Substrates on Cotton & Cellulose Fibers. American Chemical Society Symposium Series. P. 171-185.

SHIFTING THE PH PROFILE OF ASPERGILLUS NIGER PHYA PHYTASE TO MATCH THE STOMACH PH ENHANCES ITS EFFECTIVENESS AS AN ANIMAL FEED ADDITIVE - (Peer Reviewed Journal)

Kim, T., Mullaney, E.J., Porres, J.M., Roneker, K.R., Crowe, S., Rice, S., Ko, T., Ullah, A.H., Daly, C.B., Welch, R.M., Lei, X.G. 2006. Shifting the ph profile of Aspergillus niger PhyA phytase to match the stomach ph enhances its effectiveness as an animal feed additive. Applied and Environmental Microbiology. 72(6):4397-4403.

CONSERVATION OF AN EIGHT-CYSTEINE MOTIF IN FUNGAL HISTIDINE ACID PHOSPHATASES - (Abstract)

Mullaney, E.J., Ullah, A.H. 2005. Conservation of an eight-cysteine motif in fungal histidine acid phosphatases (abstract). Mycological Society of America. p. 169.

PHYTASES: ATTRIBUTES, CATALYTIC MECHANISMS AND APPLICATIONS - (Abstract)

Mullaney, E.J., Ullah, A.H. 2005. Phytases: attributes, catalytic mechanisms and applications (abstract). Inositol Phosphates in the Plant and Soil System. p.17-18.

MONITORING OF UNFOLDING AND REFOLDING IN FUNGAL PHYTASE (PHYA) BY DYNAMIC LIGHT SCATTERING - (Peer Reviewed Journal)

Ullah, A.H., Sethumadhavan, K., Mullaney, E.J. 2005. Monitoring of unfolding and refolding in fungal phytase (phyA) by dynamic light scattering. Biochemical and Biophysical Research Communications. 327(4):993-998.

CONSERVATION OF CYSTEINE RESIDUES IN FUNGAL HISTIDINE ACID PHYTASES - (Peer Reviewed Journal)

Mullaney, E.J., Ullah, A.H., 2005. Conservation of cysteine residues in fungal histidine acid phytases. Biochemical and Biophysical Research Communications. Biochemical and Biophysical Research Communications. 328(2):404-408.

UNFOLDING AND REFOLDING STUDIES OF FUNGAL PHYTASE USING DYNAMIC LIGHT SCATTERING - (Abstract)

Ullah, A.H., Sethumadhavan, K. Unfolding and refolding studies of fungal phytase using dynamic light scattering(abstract). American Society for Biochemistry & Molecular Biology Annual Meeting 2004 FASEB Journal, Enzyme Structure, and Function. 8(18):C24.

THE TERM PHYTASE COMPRISES SEVERAL DIFFERENT CLASSES OF ENZYMES - (Peer Reviewed Journal)

Mullaney, E.J., Ullah, A.H. 2003. Phytases comprises structurally different classes of enzymes. Biochemical and Biophysical Research Communications. 312(1):179-184.

FUNGAL PHYA GENE EXPRESSED IN POTATO LEAVES PRODUCES ACTIVE AND STABLE PHYTASE - (Peer Reviewed Journal)

Ullah, A.H., Sethumadhavan, K., Mullaney, E.J., Ziegelhoffer, T., Austin-Phillips, S. 2003. Fungal phyA gene expressed in potato leaves produces active and stable phytase. Biochemical and Biophysical Research Communications. 306(2):603-609.

PHYA GENE PRODUCT OF ASPERGILLUS FICUUM AND PENIOPHORA LYCII PRODUCES TWO DISSIMILAR PHYTASES - (Peer Reviewed Journal)

Ullah, A.H., Sethumadhavan, K. 2003. Phya gene product of aspergillus ficuum and peniophora lycii produces two dissimilar phytases. Biochemical and Biophysical Research Communications. 303(2):463-468.

ALTERATION OF THE PH VERSUS ACTIVITY PROFILE OF FUNGAL PHYTASE BY SITE-DIRECTED MUTAGENESIS - (Abstract)

Ullah, A.H., Mullaney, E.J. 2003. Alteration of the ph versus activity profile of fungal phytase by site-directed mutagenesis (abstract). European Symposium of the Protein Society.

STRUCTURAL REFINEMENT OF FUNGAL PHYTASE (PHY A)FOR ALTERATION OF PH OPTIMUM - (Abstract)

Ullah, A.H., Mullaney, E.J. 2002. Structural refinement of fungal phytase (phy a)for alteration of ph optimum (Abstract). Protein Structure.