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United States Department of Agriculture

Agricultural Research Service

Related Topics

PAUL W TOOLEY
Foreign Disease-Weed Science
Supervisory Research Plant Pathologist

Phone: (301) 619-2632
Fax: (301) 619-2880
Room 202
1301 DITTO AVENUE
BLDG 1301
FORT DETRICK, MD, 21702-5023

Projects
EMERGING FOREIGN FUNGAL PLANT PATHOGENS: DETECTION, BIOLOGY, AND INTERACTIONS WITH HOST PLANTS
Appropriated (D)
  Accession Number: 423056
BIOLOGY, PATHOLOGY, AND EPIDEMIOLOGY OF EMERGING OOMYCETE PATHOGENS
Appropriated (D)
  Accession Number: 423057

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
The necrosis-inducing Phytophthora protein gene family of Phytophthora capsici is involved in pathogenicity - (Peer Reviewed Journal)
Feng, B., Sun, B., Lv, R., Fu, L., Wang, H., Zhu, J., Wu, Q., Tooley, P.W., Zhang, X. 2014. The necrosis-inducing Phytophthora protein gene family of Phytophthora capsici is involved in pathogenicity. Biomed Central (BMC) Plant Biology. 14:126.
Effects of inoculum density and wounding on stem infection of three Eastern U.S. forest species by Phytophthora ramorum - (Peer Reviewed Journal) - (24-Feb-14)
The effect of temperature on germination of chlamydospores of Phytophthora ramorum - (Peer Reviewed Journal)
Tooley, P.W., Browning, M.E., Leighty, R.M. 2014. The effect of temperature on germination of chlamydospores of Phytophthora ramorum. Mycologia. 106:424-430.
Inoculum density relationships for infection of some Eastern forest species by Phytophthora ramorum - (Peer Reviewed Journal)
Tooley, P.W., Browning, M.E., Leighty, R.M. 2013. Inoculum density relationships for infection of some Eastern forest species by Phytophthora ramorum. Journal of Phytopathology. 161:595-603.
Molecular cloning and functional analysis of three genes encoding polygalacturonase-inhibiting proteins from Capsicum annuum, and their relation to increased resistance to two fungal pathogens - (Peer Reviewed Journal)
Wang, X., Zhu, X., Tooley, P.W., Zhang, X. 2013. Molecular cloning and functional analysis of three genes encoding polygalacturonase-inhibiting proteins from Capsicum annuum, and their relation to increased resistance to two fungal pathogens. Plant Physiology. 81:379-400.
Enhanced recovery of Phytophthora ramorum from soil following 30 days storage at 4C - (Peer Reviewed Journal)
Tooley, P.W., Carras, M.M. 2011. Enhanced recovery of Phytophthora ramorum from soil following 30 days storage at 4C. Journal of Phytopathology. 159:641-643.
Inoculum density effects on infection of selected Eastern US forest species by Phytophthora ramorum - (Abstract Only)
Tooley, P.W., Browning, M.E. 2011. Inoculum density effects on infection of selected Eastern US forest species by Phytophthora ramorum. Phytopathology. 101:S274-275.
Infectivity and sporulation of Phytophthora ramorum on northern red oak and chestnut oak - (Peer Reviewed Journal)
Tooley, P.W., Browning, M.E., Leighty, R.M. 2011. Infectivity and sporulation of Phytophthora ramorum on northern red oak and chestnut oak. Journal of Phytopathology. 159:516-521.
Isolation of nine Phytophthora capsici pectin methylesterase genes which are differentially expressed in various plant species - (Peer Reviewed Journal)
Li, P., Feng, B., Wang, H., Tooley, P.W., Zhang, X. 2011. Isolation of nine Phytophthora capsici pectin methylesterase genes which are differentially expressed in various plant species. Journal of Basic Microbiology. 51:61-70.
Detection of Phytophthora ramorum chlamydospores in soil by baiting and dilution plating - (Abstract Only)
Tooley, P.W., Carras, M.M. 2010. Detection of Phytophthora ramorum chlamydospores in soil by baiting and dilution plating. Phytopathology. 100:S208.
Real-time PCR detection of sorghum ergot pathogens Claviceps africana, C. sorghi, and C. sorghicola - (Peer Reviewed Journal)
Tooley, P.W., Carras, M.M., Sechler, A.J., Rajasab, A.H. 2010. Real-time PCR detection of sorghum ergot pathogens Claviceps africana, C. sorghi, and C. sorghicola. Journal of Phytopathology. doi: 10.1111/j.1439-0434.2010.01683.x
Adaptation of a Phytophthora ramorum Real-Time Polymerase Chain Reaction Assay based on a mitochondrial gene region for use on the Cepheid SmartCycler - (Peer Reviewed Journal)
Sechler, K.E., Carras, M.M., Shishkoff, N., Tooley, P.W. 2010. Adaptation of a Phytophthora ramorum Real-Time Polymerase Chain Reaction Assay based on a mitochondrial gene region for use on the Cepheid SmartCycler. Plant Health Progress. 2010 - 0212-01-RS.
Recovery Plan for Red Leaf Blotch of Soybean Caused by Phoma glycinicola Reprint Icon - (Government Publication)
Hartman, G.L., Haudenshield, J.S., Smith, K.L., Tooley, P.W., Shelton, J., Bulluck, R., Engle, J., Magarey, R., Royer, M., Sutker, E., Cardwell, K., Herman, T., Datnoff, L. 2009. Recovery Plan for Red Leaf Blotch of Soybean Caused by Phoma glycinicola. Government Publication/Report. http://www.ars.usda.gov/SP2UserFiles/Place/00000000/opmp/Soybean%20RLB%20FINAL%20July%202009.pdf. pp. 4-21.
Factors Affecting Onset of Sporulation in Phytophthora ramorum - (Abstract Only)
Tooley, P.W., Browning, M.E. 2010. Factors Affecting Onset of Sporulation in Phytophthora ramorum. Sudden Oak Death Science Symposium. 2010: 373-374.
The effect of temperature and moisture period on infection of Rhododendron Cunningham’s White by Phytophthora ramorum - (Peer Reviewed Journal)
Tooley, P.W., Browning, M.E., Kyde, K.L., Berner, D.K. 2009. The effect of temperature and moisture period on infection of Rhododendron Cunningham’s White by Phytophthora ramorum. Phytopathology. 99:1045-1052.
Sporulation capacity of Phytophthora ramorum on northern red oak and chestnut oak - (Abstract Only)
Tooley, P.W., Browning, M.E. 2009. Sporulation capacity of Phytophthora ramorum on northern red oak and chestnut oak. Phytopathology. 99:S05.
Standardizing the Nomenclature for Clonal Lineages of the Sudden Oak Death Pathogen, Phytophthora ramorum - (Peer Reviewed Journal)
Grunwald, N.J., Goss, E.M., Ivors, K., Garbelotto, M., Martin, F.N., Prospero, S., Hansen, E., Bonants, P., Hamelin, R., Chastagner, G., Werres, S., Rizzo, D.M., Abad, G., Beales, P., Bilodeau, G.J., Blomquist, C., Brasier, C., Briere, S., Chandelier, A., Davidson, J.M., Denman, S., Elliott, M., Fichtner, E.J., Frankel, S.J., Goheen, E.M., De Gruyter, H., Heungens, K., James, D., Kanaskie, A., Mcwilliams, M., Moralejo, E., Osterbauer, N.K., Palm, M.E., Parke, J.L., Shamoun, S.F., Shishkoff, N., Tooley, P.W., Vettraino, A., Webber, J., Widmer, T.L. 2009. Standardizing the nomenclature for clonal lineages of the sudden oak death pathogen, Phytophthora ramorum. Phytopathology. 99:792-795.
Evaluation of molecular markers for Phytophthora ramorum detection and identification; testing for specificity using a standardized library of isolates. - (Peer Reviewed Journal)
Martin, F.N., Coffey, M., Berger, P., Hamelin, R.C., Tooley, P.W., Garbelotto, M., Hughes, K., Kubisiak, T. 2009. Evaluation of molecular markers for Phytophthora ramorum detection and identification; testing for specificity using a standardized library of isolates. Phytopathology 98:390-403.
Recovery Plan for Phytophthora kernoviae Causing Bleeding Trunk Cankers, Leaf Blight and Stem Dieback in Trees and Shrubs - (Government Publication)
Benson, M., Ivors, K., Koch, F., Fichtner, E., Garbelotto, M., Rizzo, D., Tjosvold, S., Hansen, E., Parke, J., Hong, C., Chastagner, G., Jeffers, S., Woodward-Williams, J., Kim, S., Britton, K., Denitto, G., Frankel, S., Micales, J., Grunwald, N.J., Martin, F.N., Shishkoff, N., Smith, K.L., Tooley, P.W., Widmer, T.L., Bulluck, R., Burnett, G., Ferguson, L., Fowler, G., Garrett, L., Jones, J., Magarey, R., Randall-Schadel, B., Cardwell, K., Draper, M., Chand-Goyal, T. 2009. Recovery Plan for Phytophthora kernoviae Causing Bleeding Trunk Cankers, Leaf Blight and Stem Dieback in Trees and Shrubs. Government Publication/Report. Available at: http://www.ars.usda.gov/SP2UserFiles/Place/00000000/opmp/P.%20kernoviae%2081100.pdf
Susceptibility of some common Eastern forest understory plant species to Phytophthora ramorum - (Peer Reviewed Journal)
Tooley, P.W., Browning, M.E. 2009. Susceptibility of some common Eastern forest understory plant species to Phytophthora ramorum. Plant Disease. 93:249-256.
Development and Validation of a Tissue based Panel for the P. ramorum Proficiency Testing Program - (Abstract Only)
Mavrodieva, V., Negi, S., Picton, D., Levy, L., Tooley, P.W., Shishkoff, N., Luster, D.G. 2008. Development and Validation of a Tissue based Panel for the P. ramorum Proficiency Testing Program. Phytopathology. 98:S100
Survival of Phytophthora ramorum hyphae following exposure to temperature extremes and various humidities - (Peer Reviewed Journal)
Browning, M.E., Englander, L., Tooley, P.W., Berner, D.K. 2008. Survival of Phytophthora ramorum hyphae following exposure to temperature extremes and various humidities. Mycologia. 100:236-245.
Recovery of Phytophthora ramorum following exposure to extreme temperatures - (Peer Reviewed Journal)
Tooley, P.W., Browning, M.E., Berner, D.K. 2008. Recovery of Phytophthora ramorum following exposure to extreme temperatures. Plant Disease. 92:431-437
Real-time PCR detection of sorghum ergot pathogens Claviceps africana, C. sorghi, and C. sorghicola - (Abstract Only)
Tooley, P.W., Carras, M.M., Sechler, A.J. 2008. Real-time PCR detection of sorghum ergot pathogens Claviceps africana, C. sorghi, and C. sorghicola. International Congress of Plant Pathology Abstracts and Proceedings. Journal of Plant Pathology. Vol. 90 (2, Supplement). 52.312.2008
Survival of Phytophthora ramorum chlamydospores at high and low temperatures - (Abstract Only)
Tooley, P.W., Browning, M.E. 2008. Survival of Phytophthora ramorum chlamydospores at high and low temperatures. Page 475 in: Frankel, Susan J; Kliejunas, John T; Palmieri, Katherine M., tech. coords. 2008. Proceedings of the sudden oak death third science symposium. Gen. Tech. Rep. PSW-GTR-214. Albany, CA: U.S. Department of Agriculture, Forest Service, Pacific Southwest Research Station. 491 p.
Genetic and Morphological Diversity of Temperate and Tropical Isolates of Phytophthora capsici - (Peer Reviewed Journal)
Bowers, J.H., Martin, F.N., Tooley, P.W., Luz, E. 2007. Genetic and Morphological Diversity of Temperate and Tropical Isolates of Phytophthora capsici. Phytopathology 97:492-503. DOI:10.1094/PHYTO-97-4-0492
Susceptibility of Some Eastern Forest Species to Phytophthora Ramorum - (Peer Reviewed Journal)
Tooley, P.W., Kyde, K.L. 2007. Susceptibility of some eastern forest species to phytophthora ramorum. Plant Disease. 91:435-438
Recovery of Phytophthora Ramorum Following Exposure to Temperature Extremes - (Abstract Only)
Tooley, P.W., Browning, M.E., Englander, L. 2006. Recovery of phytophthora ramorum following exposure to temperature extremes. Phytopathology 96:S115
Growth and Sporulation of Phytophthora Ramorum in Vitro in Response to Temperature and Light - (Peer Reviewed Journal) - (29-Mar-06)
Analysis of Claviceps Africana and C. Sorghi from India Using Aflps, Ef-1alpha Gene Intron 4, and Beta-Tubulin Gene Intron 3 - (Peer Reviewed Journal)
Tooley, P.W., Bandyopadhyay, R., Carras, M.M. 2006. Analysis of claviceps africana and c. sorghi from india using aflps, ef-1alpha gene intron 4, and beta-tubulin gene intron 3. Mycological Research. 110:441-451.
Cultural Characteristics, Morphology, and Variation Within Claviceps Africana and C. Sorghi from India - (Peer Reviewed Journal) - (16-Dec-05)
Real-Time Fluorescent Pcr Detection of the Sudden Oak Death Pathogen, Phytophthora Ramorum, Using Mitochondrial Gene Regions - (Peer Reviewed Journal)
Tooley, P.W., Martin, F.N., Carras, M.M., Frederick, R.D. 2006. Real-time fluorescent pcr detection of the sudden oak death pathogen, phytophthora ramorum, using mitochondrial gene regions. Phytopathology. 95:336-345.
The Effect of Temperature on Infection of Rhododendron 'cunningham's White' by Phytophthora Ramorum - (Abstract Only)
Tooley, P.W., Browning, M.E., Kyde, K.L. 2005. The effect of temperature on infection of Rhododendron 'Cunningham's white' by Phytophthora ramorum [abstract]. Phytopathology. 95:S104.
Nep1 Orthologs Encoding the Necrosis and Ethylene Inducing Proteins Exist As a Multigene Family in Phytophthora Megakarya, Causal Agent of Black Pod Disease on Cacao - (Peer Reviewed Journal)
Bae, H., Bowers, J.H., Tooley, P.W., Bailey, B.A. 2005. Nep1 orthologs encoding the necrosis and ethylene inducing proteins exist as a multigene family in phytophthora megakarya, causal agent of black pod disease on cacao. Mycological Research. 109:1373-1385.
Prevalence of Major Diseases of Sorghum in Deccan Regions of India - (Abstract Only)
Navi, S., Bandyopadhyay, R., Tonapi, V., Rao, T., Reddy, R., Tooley, P.W., Thomas, D. 2005. Prevalence of major diseases of sorghum in deccan regions of india. Phytopathology 95:574
The Use of Mitochondrial DNA for Clarifying Phylogenetic Relationships and Isolate Identification with the Genus Phytophthora - (Abstract Only)
Martin, F.N., Tooley, P.W. 2005. The use of mitochondrial dna for clarifying phylogenetic relationships and isolate identification with the genus phytophthora. Meeting Abstract. No page numbers.
Host Range of Sorghum Ergot Pathogens C. Africana and C. Sorghi in India - (Peer Reviewed Journal)
Journal of Phytopathology 153:1-4
The Use of Mitochondrial DNA for Clarifying Phylogenetic Relationships and Isolate Identification with the Genus Phytophthora. - (Abstract Only)
Martin, F.N., Tooley, P.W. The use of mitochondrial dna for clarifying phylogenetic relationships and isolate identification with the genus phytophthora. Phytopathology. 2004. v. 94 p. S66.
Susceptibility of Selected Ericaceous Ornamental Host Species to Phytophthora Ramorum - (Peer Reviewed Journal)
Plant Disease 88:993-999
Identification of Phytophthora Isolates to Species Level Using Rflp Analysis of a Pcr Amplified Region of Mitochondrial DNA - (Peer Reviewed Journal)
Martin, F.N., Tooley, P.W. 2004. Identification of phytophthora isolates to species level using rflp analysis of a pcr amplified region of mitochondrial dna. Phytopathology. v. 94. p. 983-991.
Real-Time Fluorescent Pcr Detection of the Sudden Oak Death Pathogen Phytophthora Ramorum - (Abstract Only)
Mycologia 55:37
Persistence of Phytophthora Ramorum in Nursery Plants and Soil - (Abstract Only)
Shishkoff, N., Tooley, P.W. 2004. Persistence of Phytophthora ramorum in nursery plants and soil. Phytopathology. 94:S95. Publication no. P-2004-0649-AMA.
Rapid Identification of Phytophthora Ramorum Using Pcr-Sscp Analysis of Ribosomal DNA Its-1 - (Peer Reviewed Journal)
Letters in Applied Microbiology 38:433-439
Molecular Detection of Phytophthora Ramorum and a Phytophthora Ilicis-Like Species Associated with Sudden Oak Death in California - (Peer Reviewed Journal)
Martin, F.N., Tooley, P.W., Blomquist, C. 2004. Molecular detection of phytophthora ramorum and a phytophthora ilicis-like species associated with sudden oak death in california. Phytopathology. v. 94. p. 621-631.
Phylogenetic Relationships of Phytophthora Ramorum and a Phytophthora Ilicis-Like Species Associated with Sudden Oak Death in California - (Peer Reviewed Journal)
Martin, F.N., Tooley, P.W. 2003. Phylogenetic relationships of phytophthora ramorum and a phytophthora ilicis-like species associated with sudden oak death in california. Mycological Research. v. 107. p. 1379-1391.
Prevalence of Sorghum Ergot in Southeast Asia - (Peer Reviewed Journal)
Tonapi, V.A., Wirojwattanakul, K., Vinh, D.V., Thein, M.M., Navi, S.S., Bandyopadhyay, R., Pande, S., Tooley, P.W. 2004. Prevalence of sorghum ergot in southeast asia. International Sorghum and Millets Newsletter. 44:95-97.
Development of a Molecular Marker System for Detection of Phytophthora Spp. and Diagnosis of Species Associated with Sudden Oak Death in California. - (Abstract Only)
Martin, F.N., Tooley, P.W., Frederick, R.D. 2003. Development of a molecular marker system for detection of phytophthora spp. and diagnosis of species associated with sudden oak death in california.. American Phytopathological Society Annual Meeting. Phytopathology v. 93. p. S58.
Factors Influencing Growth and Sporulation of Phytophthora Ramorum, Causal Agent of `sudden Oak Death' - (Abstract Only)
BROWNING, M., ENGLANDER, L., TOOLEY, P.W. FACTORS INFLUENCING GROWTH AND SPORULATION OF PHYTOPHTHORA RAMORUM, CAUSAL AGENT OF `SUDDEN OAK DEATH.' Phytopathology. 93:S12. 2003.
Plant Hosts in the Nursery - (Other)
ENGLANDER, L., TOOLEY, P.W. PLANT HOSTS IN THE NURSERY. WORLD WIDE WEB. 2003.
Intraspecific Variation in Claviceps Africana - (Book / Chapter)
pp.151-155 In: Sorghum and Millet Diseases (J. F. Lester, ed.) Iowa State Press, Ames, 1A.504pp.
Phylogenetic Relationships among Phytophthora Species Inferred from Sequence Analysis of the Mitochondrially-Encoded Cytochrome Oxidase I and II Genes - (Peer Reviewed Journal)
MARTIN, F.N., TOOLEY, P.W. PHYLOGENETIC RELATIONSHIPS AMONG PHYTOPHTHORA SPECIES INFERRED FROM SEQUENCE ANALYSIS OF THE MITOCHONDRIALLY-ENCODED CYTOCHROME OXIDASE I AND II GENES. MYCOLOGIA. 95:269-284. 2003.
Aflp Comparisons among Claviceps Africana Isolates Causing Sorghum Ergot in the U.S., Africa, India, and Japan - (Peer Reviewed Journal)
Tooley, P.W., Goley, E.D., Carras, M. M., Oneill, N.R. AFLP comparisons among claviceps africana isolates from the U.S., Mexico, Africa, Australia, India, and Japan. Plant Disease. 86:1247-1252. 2002.
Aflp Comparisons among Claviceps Africana Isolates Causing Sorghum Ergot in the U.S., Africa, India, and Japan - (Peer Reviewed Journal)
Tooley, P.W., Goley, E.D., Carras, M. M., Oneill, N.R. AFLP comparisons among claviceps africana isolates from the U.S., Mexico, Africa, Australia, India, and Japan. Plant Disease. 86:1247-1252. 2002.
Aflp Comparisons among Claviceps Africana Isolates Causing Sorghum Ergot in the U.S., Africa, India, and Japan - (Peer Reviewed Journal)
Tooley, P.W., Goley, E.D., Carras, M. M., Oneill, N.R. AFLP comparisons among claviceps africana isolates from the U.S., Mexico, Africa, Australia, India, and Japan. Plant Disease. 86:1247-1252. 2002.
Aflp Comparisons among Claviceps Africana Isolates Causing Sorghum Ergot in the U.S., Africa, India, and Japan - (Peer Reviewed Journal)
Tooley, P.W., Goley, E.D., Carras, M. M., Oneill, N.R. AFLP comparisons among claviceps africana isolates from the U.S., Mexico, Africa, Australia, India, and Japan. Plant Disease. 86:1247-1252. 2002.
Prevalence of Ergot of Sorghum in India - (Peer Reviewed Journal)
NAVI, S.S., BANDYOPADYAY, R., TONAPI, V.A., NAGESHWAR RAO, T.G., TOOLEY, P.W., REDDY, R.K., INDIRA, S., PANDE, S. PREVALENCE OF ERGOT OF SORGHUM IN INDIA. INTERNATIONAL SORGHUM AND MILLETS NEWSLETTER. 43:70-71. 2002.
An Outbreak of Sorghum Ergot in Parts of Andhra Pradesh, India - (Peer Reviewed Journal)
NAVI, S.S., BANDYOPADYSY, R., NAGESWARA RAO, T.G., TOOLEY, P.W. AN OUTBREAK OF SORGHUM ERGOT IN PARTS OF ANDHRA PRADESH, INDIA. INTERNATIONAL SORGHUM AND MILLETS NEWSLETTER. 43:68-70. 2002.
Use of Ligase Chain Reaction for Detection of Phytophthora Infestans in Potatoes - (Peer Reviewed Journal) - (08-Apr-02)
Infectivity of Phytophthora Ramorum on Selected Ericaceous Host Species - (Abstract Only)
TOOLEY, P.W., ENGLANDER, L. INFECTIVITY OF PHYTOPHTHORA RAMORUM ON SELECTED ERICACEOUS HOST SPECIES. Phytopathology. 92:S81. 2002.
Analysis of Claviceps Africana and C. Sorghi from India Using Aflps, Ef-1 Alpha Gene Intron 4, and Beta-Tubulin Gene Intron 3 - (Book / Chapter)
BANDYOPADHYAY, R., MUTHUSUBRAMANIAN, V., TOOLEY, P.W., CHAKRABORTY, S., PAZOUTOVA, S., NAVI, S.S. ANALYSIS OF CLAVICEPS AFRICANA AND C. SORGHI FROM INDIA USING AFLPS, EF-1 ALPHA GENE INTRON 4, AND BETA-TUBULIN GENE INTRON 3. BOOK CHAPTER. pp75-79. IN Sorghum and Millet Diseases, J.F. Leslie, ed, Iowa State Press, Ames, IA 504pp.2002.
Pcr-Rflp Analysis As An Alternative to Morphological Criteria in Phytophthora Species Identification - (Peer Reviewed Journal) - (13-Sep-99)
The Use of An Oligonucleotide That Hybridizes to DNA Sequences Interspersedin the Genome of Members of the Genus Phytophthora As An Adjunct to Morphological Criteria in Species Identification - (Peer Reviewed Journal) - (20-Aug-98)
Somatic Hybrids Between Solanum Bulbocastanum and Potato: a New Source of Resistance to Late Blight - (Peer Reviewed Journal) - (10-Jun-98)
Extraction of DNA from Phytophthora Infestans Using Qiagen Columns - (Peer Reviewed Journal) - (19-Apr-96)
Relationships among Group IV Phytophthora Species Inferred by Restriction Analysis of the Its2 Region - (Peer Reviewed Journal) - (19-Apr-96)
Presence of Tyl-Copia Group Retrotransposon Sequences in the Potato Late Blight Pathogen Phytophthora Infestans - (Peer Reviewed Journal) - (07-Feb-96)
Isolation of a Species-Specific Mitochondrial DNA Sequence for Identification of Tilletia Indica, the Karnal Bunt of Wheat Fungus - (Peer Reviewed Journal) - (24-Oct-95)
ARS News Articles
Last Modified: 8/19/2014
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