Projects

BIOPRODUCTS FROM AGRICULTURAL FEEDSTOCKS Appropriated (D)   Accession Number: 420235

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Physical characteristics of genetically-altered wheat related to technological protein separation - (Peer Reviewed Journal)

Robertson, G.H., Blechl, A.E., Hurkman Ii, W.J., Anderson, O.D., Cao, T., Tanaka, C.K., Gregorski, K.S., Orts, W.J. 2013. Physical characteristics of genetically-altered wheat related to technological protein separation . Cereal Chemistry. 90(1): 1-12.

Differences in alcohol-soluble protein from genetically altered wheat using capillary zone electrophoresis, one- and two-dimensional electrophoresis and a novel gluten matrix association factor analysis. - (Peer Reviewed Journal)

Robertson, G.H., Hurkman Ii, W.J., Anderson, O.D., Tanaka, C.K., Cao, T., Orts, W.J. 2012. Characterizing alcohol-soluble protein from genetically-altered wheat. Cereal Chemistry. dx.doi.org/101094/cchem-10-11-0123.

Wheat flour exposed to ethanol yields dough with unexpected properties - (Peer Reviewed Journal)

Robertson, G.H., Hurkman Ii, W.J., Cao, T., Gregorski, K.S., Tanaka, C.K., Glenn, G.M., Orts, W.J. 2011. Wheat flour exposed to ethanol yields dough with unexpected properties. Journal of Cereal Science. DOI:10.1094/CCHEM-03-11-0041.

Ethanol in biorefining and dehydration of agricultural materials: energy, capital cost, and product quality implications - (Peer Reviewed Journal)

Robertson, G.H., Offeman, R.D., Cao, T., Orts, W.J. 2011. Ethanol in biorefining and dehydration of agricultural materials: energy, capital cost, and product quality implications. Biofuels, Bioproducts, & Biorefining (Biofpr). 5:37-53.

Finding the Bio in Biobased Products: Electrophoretic Identification of Wheat Proteins in Processed Products - (Peer Reviewed Journal)

Robertson, G.H., Hurkman Ii, W.J., Cao, T., Tanaka, C.K., Orts, W.J. 2010. Finding the Bio in Biobased Products: Electrophoretic Identification of Wheat Proteins in Processed Products. Journal of Agricultural Food & Chemistry. 2010 April 14;58(7):4169-79.

Extraction of ethanol with higher carboxylic acid solvents and their toxicity to yeast - (Peer Reviewed Journal)

Offeman, R.D., Franquivillanueva, D.M., Cline, J.L., Robertson, G.H., Orts, W.J. 2010. Extraction of ethanol with higher carboxylic acid solvents and their toxicity to yeast. Separation and Purification Technology. 72: 180-185.

Chromosomal integration of recombinant alpha-amylase and glucoamylase genes in saccharomyces cerevisiae for starch conversion - (Peer Reviewed Journal)

Wong, D., Batt Throne, S.B., Robertson, G.H., Lee, C.C., Wagschal, K.C. 2010. Chromosomal integration of recombinant alpha-amylase and glucoamylase genes in saccharomyces cerevisiae for starch conversion. Industrial Biotechnology. 6:112-118.

Water absorbance and thermal properties of sulfated wheat gluten films - (Peer Reviewed Journal)

Chiou, B., Robertson, G.H., Rooff, L.E., Cao, T., Jafri, H.H., Gregorski, K.S., Imam, S.H., Glenn, G.M. 2010. Water absorbance and thermal properties of sulfated wheat gluten films. Journal of Applied Polymer Science. 116: 2638-2644.

Cloning and characterization of an alpha-glucuronidase from a mixed microbial population - (Peer Reviewed Journal)

Lee, C.C., Kibblewhite, R.E., Wagschal, K.C., Robertson, G.H., Orts, W.J. 2009. Cloning and characterization of an alpha-glucuronidase from a mixed microbial population. Enzyme and Microbial Technology. 155(1-3):314-320.

Purification and characterization of a glycoside hydrolase family 43 Beta-xylosidase from Geobacillus thermoleovorans IT-08 - (Peer Reviewed Journal)

Wagschal, K.C., Heng, C., Lee, C.C., Robertson, G.H., Orts, W.J., Wong, D. 2008. Purification and characterization of a glycoside hydrolase family 43 Beta-xylosidase from Geobacillus thermoleovorans IT-08. Applied Biochemistry and Biotechnology. 155(1-3):1-10.

Metagenomic gene discovery and enzyme engineering for biomass conversion - (Abstract Only)

Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. Metagenomic gene discovery and enzyme engineering for biomass conversion. American Chemical Society Western Regional Meeting. American Chemical Society Symposium: New Developments in Biofuels Research. Abstract 204.

Extraction of ethanol with higher alcohol solvents and their toxicity to yeast - (Peer Reviewed Journal)

Offeman, R.D., Stephenson, S.K., Franquivillanueva, D.M., Cline, J.L., Robertson, G.H., Orts, W.J. 2008. Extraction of ethanol with higher alcohol solvents and their toxicity to yeast. Separation and Purification Technology. 63: 444-451.

Effect on dough functioned properties of partial fractionation, redistribution and in-site deposition of wheat flour gluten proteins exposed to ethanol and aqueous ethanol. - (Peer Reviewed Journal)

Robertson, G.H., Cao, T., Orts, W.J. 2008. Effect on dough functioned properties of partial fractionation, redistribution and in-site deposition of wheat flour gluten proteins exposed to ethanol and aqueous ethanol. Cereal Chemistry. 85(5): 599-606.

SPIRAL-WOUND LIQUID MEMBRANE MODULE FOR SEPARATION OF FLUIDS AND GASES - (Patent Application)

Offeman, R.D., Robertson, G.H. Spiral-wound liquid membrane module for separation of fluids and gases. U.S. Patent No. 7,341,663 Issued 03/11/2008

Electrospun Nanofibers of Poly(vinyl alcohol) Reinforced with Cellulose Nanofibrils - (Peer Reviewed Journal)

Medeiros, E.S., Mattoso, L.H., Ito, E.N., Gregorski, K.S., Robertson, G.H., Offeman, R.D., Wood, D.F., Orts, W.J. 2008. Electrospun Nanofibers of Poly(vinyl alcohol) Reinforced with Cellulose Nanofibrils. Journal of Biobased Materials and Bioenergy. 231-242.

Electrospun nanofibers of poly (vinyl alcohol) reinforced with cellulose nanofibrils - (Peer Reviewed Journal)

Medeiros, E.S., Mattoso, L.H., Ito, E.N., Gregorski, K.S., Robertson, G.H., Offeman, R.D., Wood, D.F., Orts, W.J. 2008. Electrospun nanofibers of poly (vinyl alcohol) reinforced with cellulose nanofibrils. Polymer Engineering & Science. 2(3):231-242.

Cloning, Expression and Characterization of a Glycoside Hydrolase Family 39 Xylosidase from Bacillus Halodurans C-125 - (Peer Reviewed Journal)

Wagschal, K.C., Franquivillanueva, D.M., Lee, C.C., Robertson, G.H., Wong, D. Cloning, Expression and Characterization of a Glycoside Hydrolase Family 39 Xylosidase from Bacillus Halodurans C-125. Applied Biochemistry and Biotechnology.

Wheat proteins extracted from flour and batter with aqueous ethanol at subambient temperatures. - (Peer Reviewed Journal)

Robertson, G.H., Cao, T., Orts, W.J. 2007. Wheat proteins extracted from flour and butter with aqueous ethanol at subambient temperatures. Cereal Chemistry. 84(5): 497-501.

Hydrogen-bond networks in linear, branched and tertiary alcohols - (Peer Reviewed Journal)

Stephenson, S.K., Offeman, R.D., Robertson, G.H., Orts, W.J. 2007. Hydrogen-bond networks in linear, branched and tertiary alcohols. Chemical Engineering Science. 62: 3019-3031.

Developing enzyme systems for biomass deconstruction - (Abstract Only)

Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. 2007. Developing enzyme systems for biomass deconstruction. Meeting Abstract. Paper No. 217

GENETIC AND BIOCHEMICAL CHARACTERIZATION OF A NOVEL BACTERIAL A-L-ARABINOFURANOSIDASE - (Peer Reviewed Journal)

Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Accinelli, R., Robertson, G.H., Wong, D. 2007. Genetic and biochemical characterization of a novel bacterial a-l-arabinofuranosidase. Enzyme and Microbial Technology. (40):747-753.

EVOLUTIONARY ENZYME DESIGN FOR IMPROVED BIOREFINING OF CROPS AND RESIDUES - (Abstract Only)

Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Robertson, G.H., Orts, W.J. 2006. Evolutionary enzyme design for improved biorefining of crops and residues. Technical Program (abstract). P8.

Ethanol and water capacities of alcohols: a molecular dynamics study - (Peer Reviewed Journal)

Stephenson, S.K., Offeman, R.D., Robertson, G.H., Orts, W.J. 2006. Ethanol and water capacities of alcohols: a molecular dynamics study. Chemical Engineering Science. 61: 5834-5840.

SYNERGISTIC ACTION OF BARLEY ALPHA-AMYLASE AND LENTINULA EDODES GLUCOAMYLASE ON RAW STARCH HYDROLYSIS - (Abstract Only)

Wong, D., Robertson, G.H., Lee, C.C., Wagschal, K.C. 2006. Synergistic action of barley alpha-amylase and lentinula edodes glucoamylase on raw starch hydrolysis. Approved 10/27/2005. American Society of Biochemistry & Molecular Biology, San Francisco, CA. April 1-4, 2006, Program 73.16, vol A27.

CLONING AND CHARACTERIZATION OF COLD-ACTIVE XYLANASE ENZYMES - (Abstract Only)

Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of cold-active xylanase enzymes. 28th Symposium on Biotechnology for Fuels and Chemicals. Paper No. 1A-41.

CLONING AND CHARACTERIZATION OF A COLD ACTIVE XYLANASE ENZYME - (Peer Reviewed Journal)

Lee, C.C., Accinelli, R., Wagschal, K.C., Robertson, G.H., Wong, D. 2006. Cloning and characterization of a cold active xylanase enzyme. Extremophiles. 10:295-300.

Solvent extraction of ethanol from aqueous solutions using biobased oils, alcohols and esters - (Peer Reviewed Journal)

Offeman, R.D., Stephenson, S.K., Robertson, G.H., Orts, W.J. 2006. Solvent extraction of ethanol from aqueous solutions using biobased oils, alcohols and esters. Journal of the American Oil Chemists' Society. 83: 153-157.

NATIVE OR RAW STARCH DIGESTION - (Review Article)

Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R., Orts, W.J. 2006. Native or raw starch digestion: A Key step in energy efficient biorefining of grain. Journal of Agricultural and Food Chemistry, 54:353-65.

THERMO-FORMED WHEAT GLUTEN PROTEIN BIOPOLYMERS - (Peer Reviewed Journal)

Pallos, F.M., Robertson, G.H., Pavlath, A.E., Orts, W.J. 2006. Thermo-formed wheat gluten protein biopolymers. Journal of Agricultural and Food Chemistry. 54:349-52.

ISOLATION AND CHARACTERIZATION OF A PSYCHROPHILIC XYLANASE ENZYME FROM FLAVOBACTERIUM SP. - (Peer Reviewed Journal)

Lee, C.C., Smith, M.R., Accinelli, R., Williams, T.G., Wagschal, K.C., Wong, D., Robertson, G.H. 2006. Isolation and characterization of a psychrophilic xylanase enzyme from flavobacterium sp. Current Microbiology, 52(2):112-116.

CHANGES IN SECONDARY PROTEIN STRUCTURES DURING MIXING DEVELOPMENT OF HIGH ABSORPTION (90%) WATER AND FLOUR MIXTURES - (Peer Reviewed Journal)

Robertson, G.H., Gregorski, K.S., Cao, T. 2005. Changes in secondary protein structures during mixing development of high absorption (90%) flour and water mixtures. Cereal Chemistry, 83:136-42.

CHARACTERIZATION OF ACTIVE LENTINULA EDODES GLUCOAMYLASE EXPRESSED AND SECRETED BY SACCHAROMYCES CEREVISIAE - (Peer Reviewed Journal)

Wong, D., Batt, S.B., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2005. Characterization of active lentinula edodes glucoamylase expressed and secreted by saccharomyces cerevisiae. Protein Journal. vol 24, pp 455-463.

Solvent extraction of ethanol from aqueous solutions. 2. Linear, branched and ring-containing alcohol solvents - (Peer Reviewed Journal)

Offeman, R.D., Stephenson, S.K., Robertson, G.H., Orts, W.J. 2005. Solvent extraction of ethanol from aqueous solutions. 2. Linear, branched and ring-containing alcohol solvents. Industrial and Engineering Chemistry Research. 44: 6797-6803.

Solvent extraction of ethanol from aqueous solutions. 1. Screening methodology for solvents - (Peer Reviewed Journal)

Offeman, R.D., Stephenson, S.K., Robertson, G.H., Orts, W.J. 2005. Solvent extraction of ethanol from aqueous solutions. 1. Screening methodology for solvents. Industrial and Engineering Chemistry Research. 44: 6789-6796.

COLD-ETHANOL FOR BIOREFINING - (Abstract Only)

Robertson, G.H. 2005. Cold-ethanol for biorefining (abstract). 4th International Starch Technology Conference. Paper No. 2.

CHARACTERIZATION AND DIRECTED EVOLUTION OF HEMICELLULOTIC ENZYMES USING NATURAL XYLOOLIGOACCHARIDE SUBSTRATES - (Abstract Only)

Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Robertson, G.H., Wong, D.W.S. 2005. Characterization of hemicellulotic degrading enzymes using natural substrates. Symposium on Biotechnology for Fuels and Chemicals, Denver, CO. Paper No. 1B-65. Abstract A10, Enzyme Engineering XVIII.

COLD ETHANOL FOR BIOREFINING - (Proceedings)

Robertson, G.H., Orts, W.J. 2005. Cold ethanol for biorefining. International Starch Technology Conference. June 5-8, 2005, Bioproducts, Editors Rausch, K., Singh, V., Tumbleson, M. University of Illinois, Urbana, IL. pp 3-3.

PROTEIN SOLUBILITY CONSIDERATIONS FOR LOW TEMPERATURE PROCESSING WITH AQUEOUS ETHANOL - (Abstract Only)

Robertson, G.H., Cao, T. 2005. Protein solubility considerations for low temperature processing with aqueous ethanol. American Association of Cereal Chemists Annual Meeting, September 11-14, 2005, Orlando. FL. Paper No. 0-76.

ALTERATION OF WHEAT FUNCTIONAL PROPERTIES BY EXTRACTIVE AND NON-EXTRACTIVE CONTACT WITH ETHANOL - (Abstract Only)

Robertson, G.H., Cao, T. 2005. Alteration of wheat functional properties by extractive and non-extractive contact with ethanol. American Association of Cereal Chemists Annual Meeting. September 11-14, 2005, Orlando, FL. Paper No. P-229.

ENZYME-COUPLED ASSAY FOR BETA-XYLOSIDASE HYDROLYSIS OF NATURAL SUBSTRATES - (Peer Reviewed Journal)

Wagschal, K.C., Franqui Espiet, D., Lee, C.C., Robertson, G.H., Wong, D. 2005. Enzyme-coupled assay for beta-xylosidase hydrolysis of natural substrates. Applied and Environmental Microbiology. 71:5318-5323.

EVOLVED ENZYMES FOR COLD OR NON-COOKING HYDROLYSIS OF STARCHES TO FERMENTATION FEEDSTOCK - (Abstract Only)

Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C., Smith, M.R. 2005. Evolved enzymes for cold or non-cooking hydrolysis of starches to fermentation feedstock. In Proceedings of the North American Grain Congress, February 20-22, 2005, Reno, NV. 2005 CDROM.

EXPRESSION AND CHARACTERIZATION OF ACTIVE LENTINUS EDODES GLUCOAMYLASE IN SACCHAROMYCES CEREVISIAE - (Abstract Only)

Wong, D., Batt, S.B., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2005. Expression and characterization of active. lentinus edodes glucoamylase in saccharomyces cerevisiae. Presentation 04:05. Paper No 11277.

ETHANOL-BASED WHEAT REFINING FOR BIOPRODUCTS - (Abstract Only)

Robertson, G.H., Cao, T. 2005. Ethanol-based wheat refining for bioproducts [abstract]. In: Proceedings of the North American Grain Congress, February 20-22, 2005, Reno, NV. CDROM.

THERMOFORMED BIOPOLYMERS FROM WHEAT GLUTEN PROTEIN - (Abstract Only)

Pallos, F.M., Robertson, G.H., Pavlath, A.E. 2004. Thermoformed biopolymers from wheat gluten protein (abstract). United States-Japan Cooperative Program in Natural Resources. Paper No. 6.

CLONING AND CHARACTERIZATION OF BIOMASS DEGRADING ENZYMES - (Abstract Only)

Lee, C.C., Accinelli, R., Batt, S.B., Williams, T.G., Wong, D., Wagschal, K.C., Robertson, G.H. 2005. Cloning and characterization of biomass degrading enzymes. Symposium on Biotechnology for Fuels and Chemicals, Denver, CO. Paper No. IB-41 (Abstract).

THERMOFORMED BIOPOLYMERS FROM WHEAT PROTEIN - (Proceedings)

Pallos, F.M., Robertson, G.H., Pavlath, A.E. 2004. Thermoformed biopolymers from wheat protein. In: Proceedings of the United States-Japan Cooperative Program in Natural Resources. December 11-18, 2004, Honolulu, HI. p.72-75.

RAW STARCH DIGESTION OR NON-COOKING BREAKDOWN OF STARCH PRIOR TO FERMENTATION - (Abstract Only)

Robertson, G.H., Wong, D. 2005. Raw starch digestion or non-cooking breakdown of starch prior to fermentation. Fuel Ethanol Workshop and Trade Show, June 28, 2005, Kansas City, MO. Paper No.8:1.

DIRECTED EVOLUTION OF BARLEY ALPHA-AMYLASE FOR INCREASED ACTIVITY - (Abstract Only)

Batt, S.B., Wong, D., Lee, C.C., Wagschal, K.C., Robertson, G.H. 2004. Directed evolution of barley alpha-amylase for increased activity. American Chemical Society Western Regional Meeting, Sacramento, CA. Paper No. 144 (Abstracts).

HETEROGENEOUS BIOCATALYIC LIQUEFACTION AND SACCHARIFICAATION OF STARCH FOR ETHANOL - (Abstract Only)

Robertson, G.H., Wong, D., Lee, C.C., Wagschal, K.C. 2004. Heterogeneous biocatalyic liquefaction and saccharificaation of starch for ethanol. Corn Utilization Conference Proceedings, June 6-9, 2004, Indianapolis, Indiana, Abstract.

IN VITRO EVOLUTION OF HIGH-ACTIVITY BARLEY ALPHA-AMYLASE FOR RAW STARCH DIGESTION - (Abstract Only)

Wong, D., Robertson, G.H., Batt, S.B., Lee, C.C. 2004. In vitro evolution of high-activity barley alpha-amylase for raw starch digestion. American Association of Cereal Chemists Meetings, September 19-22, 2004, San Diego, California. Abstract.

HIGH-ACTIVITY BARLEY ALPHA-AMYLASE BY DIRECTED EVOLUTION - (Peer Reviewed Journal)

Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. 2004. High-activity barley alpha-amylase by directed evolution. Journal of Protein Chemistry, 23:53-460.

APPLYING COMBINATORIAL CHEMISTRY AND BIOLOGY TO FOOD RESEARCH - (Review Article)

Wong, D., Robertson, G.H. 2004. Applying combinatorial chemistry and biology to food research. Journal of Agricultural and Food Chemistry, 52:7187-7198.

PROTEINS EXTRACTED BY WATER OR AQUEOUS ETHANOL DURING REFINING OF DEVELOPED WHEAT DOUGH TO VITAL WHEAT GLUTEN AND CRUDE STARCH AS DETERMINED BY CAPILLARY-ZONE ELECTROPHORESIS (CZE). - (Peer Reviewed Journal)

Robertson, G.H., Cao, T. 2004. Proteins extracted by water or aqueous ethanol during refining of developed wheat dough to vital wheat gluten and crude starch as determined by capillary-zone electrophoresis (cze). Cereal Chemistry. 81(5):673-680.

RAPID SELECTION OF ALPHA-AMYLASE MUTANTS IN YEAST LIBRARIES FOR RAW STARCH ACTIVITY - (Abstract Only)

Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. Rapid selection of alpha-amylase mutants in yeast libraries for raw starch activity. 2003. Society of Biomolecular Screening Annual Conference, Portland, Oregon.

ENGINEERING APHA-AMYLASE FOR ENHANCED ACTIVITY BY MOLECULAR EVOLUTION - (Abstract Only)

Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. Engineering apha-amylase for enhanced activity by molecular evolution. American Society of Biochemistry and Molecular Biology Annual Meeting, June 12-16, 2004, Boston, Massachusetts. Abstract No. B38.

AN EPISOMAL EXPRESSION VECTOR FOR SCREENING MUTANT GENE LIBRARIES IN PICHIA PASTORIS - (Peer Reviewed Journal)

Lee, C.C., Williams, T.G., Wong, D., Robertson, G.H. 2004. An episomal expression vector for screening mutant gene libraries in pichia pastoris. Plasmid Journal. 54(1):80-85.

DIRECT SCREENING OF LIBRARIES OF YEAST CLONES FOR ALPHA-AMYLASE ACTIVITY ON RAW STARCH - (Peer Reviewed Journal)

Wong, D., Batt, S.B., Lee, C.C., Robertson, G.H. 2004. Direct screening of libraries of yeast clones for alpha-amylase activity on raw starch. Protein and Peptide Letters. 10:459-468.

CLONING AND CHARACTERIZATION OF THE XYN11A GENE FROM LENTINULA EDODES - (Peer Reviewed Journal)

Lee, C.C., Wong, D., Robertson, G.H. 2005. Cloning and characterization of the xyn11a gene from lentinula edodes. Protein Journal. 24(1):21-26.

COMBINED ELECTROFLOTATION AND DISINFECTION IN FOOD PROCESSING - (Abstract Only)

Hernlem, B.J., Tsai, L.-S., Huxsoll, C., Robertson, G. 2000. Combined Electroflotation and Disinfection in Food Processing. Paper Number 74-12, presented at the 2000 Institute of Food Technologists Annual Meeting, June 10-14, 2000, Dallas, TX. Available: http://ift.confex.com/ift/2000/techprogram/paper_4502.htm.

PHAGE DISPLAYED PEPTIDE LIGANDS FOR PANCREATIC A-AMYLASE CROSS-REACT WITH BARLEY A-AMYLASE - (Peer Reviewed Journal) - (01-Jul-99)

LIMONOIDS AND FLAVONOIDS IN JUICES OF OROBLANCO AND MELOGOLD - (Peer Reviewed Journal) - (01-Dec-97)