Projects

PATHOGEN DETECTION AND INTERVENTION METHODS FOR SHELLFISH Appropriated (D)   Accession Number: 420935

COMPARISON OF THE SURVIVAL AND PROLIFERATION OF VIRULENT & AVIRULENT STRAINS OF VIBRIO VULNIFICUS & VIBRIO PARA. IN SEAWATER & SHELLFISH Specific Cooperative Agreement (S)   Accession Number: 419417

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Murine macrophage inflammatory cytokine production and immune activation in response to Vibrio parahaemolyticus infection - (Peer Reviewed Journal)

Waters, S., Luther, S., Joerger, T., Richards, G.P., Boyd, E., Parent, M.A. 2013. Murine macrophage inflammatory cytokine production and immune activation in response to Vibrio parahaemolyticus infection. Microbiology and Immunology. 57(4):323-328.

Resilience of norovirus GII.4 to freezing and thawing:implications for virus infectivity - (Peer Reviewed Journal)

Richards, G.P., Watson, M.A., Meade, G.K., Hovan, G.L., Kingsley, D.H. 2012. Resilience of norovirus GII.4 to freezing and thawing:implications for virus infectivity. Food and Environmental Virology. 4:192-197.

Predatory bacteria as natural modulators of Vibrio parahaemolyticus and Vibrio vulnificus in seawater and oysters - (Peer Reviewed Journal)

Richards, G.P., Fay, J.P., Dickens, K.A., Parent, M.A., Soroka, D.S., Boyd, E. 2012. Predatory bacteria as natural modulators of Vibrio parahaemolyticus and Vibrio vulnificus in seawater and oysters. Applied and Environmental Microbiology. 78:7455-7466.

Vibrio parahaemolyticus ToxRS regulator is required for stress tolerance and colonization in a novel orogastric streptomycin-induced adult murine model - (Peer Reviewed Journal)

Whitaker, B.W., Parent, M.A., Aoife, B., Richards, G.P., Boyd, F.E. 2012. Vibrio parahaemolyticus ToxRS regulator is required for stress tolerance and colonization in a novel orogastric streptomycin-induced adult murine model. Infection and Immunity. 80:1834-1845.

Light scattering sensor for real-time identification of Vibrio parahaemolyticus, V. vulnificus and V. cholera colonies on solid agar plates - (Peer Reviewed Journal)

Huff, K., Aroonnual, A., Bae, E., Banada, P., Rajwa, B., Rajwa, B., Hirleman, E.D., Robinson, J.P., Richards, G.P., Bhunia, A. 2012. Light scattering sensor for real-time identification of Vibrio parahaemolyticus, V. vulnificus and V. cholera colonies on solid agar plates. Microbial Biotechnology. DOI: 10.1111/j.1751-7915.2012.00349.x.

Water quality parameters and total aerobic bacterial and vibrionaceae loads in eastern oysters (Crassostrea virginica) from oyster gardening sites - (Peer Reviewed Journal)

Fay, J., Richards, G.P., Ozbay, G. 2012. Water quality parameters and total aerobic bacterial and vibirionaceae loads in eastern oysters (crassostrea virginica) from oyster gardening sites. Archives of Environmental Contamination and Toxicology. 64:628-637.

Critical review of norovirus surrogates in food safety research: rationale for considering volunteer studies - (Review Article)

Richards, G.P. 2012. Critical review of norovirus surrogates in food safety research: rationale for considering volunteer studies. Food and Environmental Virology. 4:6-13.

Hemocytes are sites of persistence for virus-contaminated oysters - (Peer Reviewed Journal)

Provost, K., Ozbay, G., Anderson, R., Richards, G.P., Kingsley, D.H. 2011. Hemocytes are sites of persistence for virus-contaminated oysters. Applied and Environmental Microbiology. 77:8360-8369.

Foodborne viruses - (Book / Chapter) - (23-Aug-11)

In memoriam Dean Otis Cliver 1935-2011 - (Other)

Richards, G.P. 2011. In memoriam Dean Otis Cliver 1935-2011. Food and Environmental Virology. 3(3):99-108. DOI: 10.1007/s12560-011-9064-7.

Human norovirus inactivation in oysters by high hydrostatic pressure processing: A randomized double-blinded study - (Peer Reviewed Journal)

Leon, J., Kingsley, D.H., Montes, J., Richards, G.P., Lyon, G., Abdulhafid, G., Seitz, S., Fernandez, M., Teunis, P.F., Flick, G.J., Moe, C.L. 2011. Human norovirus inactivation in oysters by high hydrostatic pressure processing: A randomized double-blinded study. Journal of Infectious Diseases. 77(15):5476-82.

Sensitive detection of multiple hepatitis A virus genotypes with a single polony-based assay - (Abstract Only) - (04-Mar-11)

Inaccuracies in predicting human norovirus inactivation using surrogate viruses - (Abstract Only)

Richards, G.P. 2011. Inaccuracies in predicting human norovirus inactivation using surrogate viruses. Journal of Shellfish Research, Volume 30, Issue 2, P. 548.

Pursuit of methods to propagate norovirus: decades of research - (Proceedings)

Richards, G.P. 2010. Pursuit of methods to propagate norovirus: decades of research. Pursuit of human norovirus propagation methods: decades of research. Edited by P.Lassus. Proceedings of the 7th International Conf. on Molluscan Shellfish Safety, Nantes, France, 14-19, June, 2009, pages 100-107

High pressure processing inactivates human norovirus within oysters - (Abstract Only)

Kingsley, D.H., Leon, J.S., Richards, G.P., Flick, G., Lyon, G.M., Abdulhafid, G., Sobolik, J., Creadore, A., Seitz, S., Smith, E., Fernandez, M., Moe, C. 2010. High pressure processing inactivates human norovirus within oysters [abstract]. Annual Meeting at American Society for Virology. p. 1.

Growth of Vibrio parahaemolyticus O3:K6 at Different Salt Concentrations Modulates Responses to pH and Temperature Stresses - (Peer Reviewed Journal)

Whitaker, B.W., Michelle, P.A., Naughton, L.M., Richards, G.P., Blumerman, S.L., Boyd, F.E. 2010. Growth of Vibrio parahaemolyticus O3:K6 at Different Salt Concentrations Modulates Responses to pH and Temperature Stresses. Applied and Environmental Microbiology. 76:720-729.

Processing Strategies to Inactivate Enteric Viruses in Shellfish: Limitations of Surrogate Viruses and Molecular Methods - (Peer Reviewed Journal)

Richards, G.P., Macleod, C., Le Guyader, F.S. 2010. Processing Strategies to Inactivate Enteric Viruses in Shellfish: Limitations of Surrogate Viruses and Molecular Methods. Food and Environmental Virology. Available: doi:10.107/s12560-010-9045-2.

Fluorogenic membrane overlays to enumerate total coliforms, Escherichia coli, and total Vibrionaceae in shellfish and seawater - (Peer Reviewed Journal)

Richards, G.P., Watson, M.A. 2010. Fluorogenic membrane overlays to enumerate total coliforms, Escherichia coli, and total Vibrionaceae in shellfish and seawater. Internationl Journal of Microbiology. Vol 2010, Article ID 910486, http://www.hindawi.com/journals/ijmb/2010/910486.html.

The Influence of Feral Horse Activity on Water and Shellfish (Gukensia demissa) Quality Along the Western Coast of Assateague Island National Seashore, Maryland - (Peer Reviewed Journal)

Lambert, M.S., Ozbay, G., Richards, G.P. 2009. The Influence of Feral Horse Activity on Water and Shellfish (Gukensia demissa) Quality Along the Western Coast of Assateague Island National Seashore, Maryland. Estuaries and Coasts - Journal of the Estuarine Research Federation. 57:405-415.

AN OVERVIEW OF ENTERIC VIRUS EXTRACTION AND ASSAY METHODS - (Proceedings)

Richards, G.P. 2009. AN OVERVIEW OF ENTERIC VIRUS EXTRACTION AND ASSAY METHODS. In:P.Busby (ed)., Proceedings of the 6th Int'l Conference on Molluscan Shellfish Safety, Blenheim, New Zealand. pp.111-118.

SHEWANELLA AND PHOTOBACTERIUM IN OYSTERS AND SEAWATER FROM THE DELAWARE BAY - (Peer Reviewed Journal)

Richards, G.P., Watson, M.A., Crane, E.J., Bushek, D., Burt, I. 2008. Shewanella and photobacterium in oysters and seawater from the delaware bay. Applied and Environmental Microbiology. 74(11):3323-3327.

AN OVERVIEW OF VIRUS EXTRACTION AND ASSAY METHODS - (Abstract Only)

Richards, G.P. 2007. An overview of virus extraction and assay methods. Abstract. 6th International Conference on Molluscan Shellfish Safety, Blenheim, New Zealand. p.78.

A SIMPLE FLUOROGENIC METHOD TO ASSESS VIBRIO CHOLERAE AND AEROMONAS HYDROPHILA IN WELL WATER - (Peer Reviewed Journal)

Richards, G.P., Watson, M.A. 2006. A simple fluorogenic method to assess vibrio cholerae and aeromonas hydrophila in well water. American Journal of Tropical Medicine and Hygiene. 65(3):516-521

DETECTION AND IDENTIFICATION OF FOODBORNE PATHOGENS TO GENUS AND SPECIES LEVELS USING A NONINVASIVE MODIFIED LIGHT SCATTEROMETER - BARDOT - (Abstract Only)

Huff, K.R., Banada, P.P., Bae, E., Bayraktar, B., Rajwa, B., Robinson, J.P., Hirleman, E.D., Richards, G.P., Bhunia, A.K. 2006. Detection and identification of foodborne pathogens to genus and species levels using a noninvasive modified light scatterometer - bardot. American Society for Microbiology. P-075.

SPECIFICITY OF A VIBRIO VULNIFICUS AMINOPEPTIDASE TOWARD KININS AND OTHER PEPTIDYL SUBSTRATES - (Peer Reviewed Journal)

Richards, G.P., Nunez, A. 2006. Specificity of a vibrio vulnificus aminopeptidase toward kinins and other peptidyl substrates. Journal of Bacteriology. Mar. 2006. p. 2056-2062.

SHELLFISH-ASSOCIATED VIRAL DISEASE OUTBREAKS - (Book / Chapter)

Richards, G.P. 2006. Shellfish-associated viral disease outbreaks. Book Chapter. In Viruses in Foods, (S.M. Goyal, Editor), Springer, New York. pp. 223-238.

THE COLONY OVERLAY PROCEDURE FOR PEPTIDASES TO DETECT AND ENUMERATE TOTAL VIBRIONACEAE IN MOLLUSCAN SHELLFISH AND THEIR GROWING WATERS - (Abstract Only)

Richards, G.P. 2005. The colony overlay procedure for peptidases to detect and enumerate total Vibrionaceae in molluscan shellfish and their growing waters. First Interstate Shellfish Safety Conference/International Conference on Molluscan Shellfish Safety Joint Workshop and Integration of Science into Policy: Special Emphasis on Vibrios and Viruses Abstract p.33.

A NOVEL, ENZYME-BASED ASSAY FOR THE RAPID AND SIMPLE DETECTION OF VIBRIOS IN SHELLFISH AND SEAWATER - (Other)

Richards, G.P., Bushek, D. 2005. A novel, enzyme-based assay for the rapid and simple detection of vibrios in shellfish and seawater. International Association for Food Protection. Abstract P4-27. p. 130.

DIVERSITY AND SEASONALITY OF THE VIBRIONACEAE IN OYSTERS (CRASSOSTREA VIRGINICA)AND SEAWATER IN DELAWARE BAY - (Abstract Only)

Richards, G.P., Burt, I.G., David, B. 2005. Diversity and seasonality of the vibrionaceae in oysters (crassostrea virginica)and seawater in delaware bay. (Abstract). National Shellfisheries Association. Pg. 46.

DEVELOPMENT OF A SIMPLE AND RAPID FLUOROGENIC PROCEDURE FOR THE IDENTIFICATION OF VIBRIONACEAE FAMILY MEMBERS - (Peer Reviewed Journal)

Richards, G.P., Watson, M.A., Parveen, S.A. 2005. Development of a simple and rapid fluorogenic procedure for the identification of vibrionaceae family members. Applied and Environmental Microbiology. 71:3524-3527.

A SURVEY FOR PHOSPHOGLUCOSE ISOMERASE WITH LYSYL AMINOPEPTIDASE ACTIVITY IN VIBRIONACEAE AND NON-VIBRIO PATHOGENS - (Peer Reviewed Journal)

Richards, G.P., Parveen, S.A. 2005. A survey for phosphoglucose isomerase with lysyl aminopeptidase activity in vibrionaceae and non-vibrio pathogens. Biochimica et Biophysica Acta. 1748:128-133.

MOLECULAR METHODS TO DETECT ENTERIC VIRUSES IN MOLLUSCAN SHELLFISH - (Proceedings)

Richards, G.P. 2004. Molecular methods to detect enteric viruses in molluscan shellfish. Proceedings of Molecular Methods to detect enteric viruses in molluscan shellfish. p.43-49.

DETECTION OF VIBRIOS IN OYSTERS CRASSOSTRTEA VIRGINICA AND SEAWATER USING A NEW, RAPID, SIMPLE, AND QUANTITATIVE ENZYME-BASED ASSAY - (Abstract Only)

Richards, G.P., Watson, M.A., Burt, I.G., Bushek, D. 2005. Detection of vibrios in oysters crassostrtea virginica and seawater using a new, rapid, simple, and quantitative enzyme-based assay. (Abstract). Aquaculture America. Paper # 8128.

STRUCTURAL AND FUNCTIONAL ANALYSES OF PHOSPHOGLUCOSE ISOMERASE FROM VIBRIO VULNIFICUS AND ITS LYSYL AMINOPEPTIDASE ACTIVITY - (Peer Reviewed Journal)

Richards, G.P. 2004. Structural and functional analyses of phosphoglucose isomerase from Vibrio vulnificus and its lysyl aminopeptidase activity. Biochimica et Biophysica Acta. 1702:89-102.

GENOGROUP I AND II NOROVIRUSES DETECTED IN STOOL BY REAL-TIME REVERSE TRANSCRIPTION-PCR USING HIGHLY DEGENERATE, "UNIVERSAL" PRIMERS - (Peer Reviewed Journal)

Richards, G.P., Watson, M.A., Frankhauser, R.L., Monroe, S.S. 2004. Genogroup I and II noroviruses detected in stool by real-time reverse transcription-PCR using highly degenerate, "universal" primers. Applied and Environmental Microbiology. Vol.70. No.12.

CHARACTERIZATION OF A LYSYL AMINOPEPTIDASE ACTIVITY ASSOCIATED WITH PHOSPHOGLUCOSE ISOMERASE OF VIBRIO VULNIFICUS - (Peer Reviewed Journal)

Richards, G.P. 2004. Characterization of a lysyl aminopeptidase activity associated with phosphoglucose isomerase of vibrio vulnificus. Biochimica et Biophysica Acta. V.1700, 2. 219-229.

MOLECULAR METHODS TO DETECT ENTERIC VIRUSES IN MOLLUSCAN SHELLFISH - (Proceedings)

Richards, G.P. 2004. Molecular methods to detect enteric viruses in molluscan shellfish. Meeting Abstract.

FOOD- AND WATER-BORNE ENTERIC VIRUSES - (Book / Chapter)

Richards, G.P. 2005. Food- and water-borne enteric viruses. Foodborne Pathogens Microbial and Mol. Biology. (P.M. Frtamico, A.K. Bhumia, J.L. Smith) Caister Academic Press. Book Chapter. pg. 121-143

INACTIVATION OF ENTERIC VIRUSES WITH INTERVENTION TECHNOLOGIES - (Abstract Only)

Richards, G.P. 2004. Inactivation of enteric viruses with intervention technologies. (Abstract). World Aquaculture Society. p. 499.

CHARACTERIZATION OF LYSYL AMINOPEPTIDASE ACTIVITY FROM PHOSPHOGLUCOSE ISOMERASE OF VIBRIO VULNIFICUS - (Peer Reviewed Journal)

Richards, G.P., Parveen, S., Hammer, C., Garfield, M. 2004. Characterization of lysyl aminopeptidase activity from phosphoglucose isomerase of vibrio vulnificus. Journal of Bacteriology. 1700(2):219-229.

A SYBR GREEN REAL-TIME RT-PCR METHOD TO DETECT AND QUANTITATE NORWALK VIRUS IN STOOLS - (Peer Reviewed Journal)

RICHARDS, G.P., WATSON, M.A., KINGSLEY, D.H. A SYBR GREEN REAL-TIME RT-PCR METHOD TO DETECT AND QUANTITATE NORWALK VIRUS IN STOOLS. JOURNAL OF VIROLOGICAL METHODS. 2004. vol. 116. pg. 67-70.

INACTIVATION OF ENTERIC VIRUSES WITH INTERVENTION TECHNOLOGIES - (Abstract Only)

Richards, G.P. 2003. Inactivation of enteric viruses with intervention technologies. (Abstract). International Association of Food Protection. p. 188.

UPTAKE, PERSISTENCE, AND LOCALIZATION OF VIRULENT AND AVIRULENT VIBRIO VULNIFICUS IN THE EASTERN OYSTER, CRASSOSTREA VIRGINICA - (Other)

PARVEEN, S., WOLFE, W.C., RICHARDS, G.P. UPTAKE, PERSISTENCE, AND LOCALIZATION OF VIRULENT AND AVIRULENT VIBRIO VULNIFICUS IN THE EASTERN OYSTER, CRASSOSTREA VIRGINICA. MEETING ABSTRACT. 2003.

THE EVOLUTION OF MOLLUSCAN SHELLFISH SAFETY - (Proceedings)

RICHARDS, G.P. THE EVOLUTION OF MOLLUSCAN SHELLFISH SAFETY. MEETING ABSTRACT. 2003. p. 221-245

PERSISTENCE OF HEPATITIS A VIRUS WITHIN OYSTERS - (Peer Reviewed Journal)

KINGSLEY, D.H., RICHARDS, G.P. PERSISTENCE OF HEPATITIS A VIRUS WITHIN OYSTERS. JOURNAL OF FOOD PROTECTION. 2003. Vol. 66 (2) p. 331-334

GLANDULAR KALLIKREINS IN THE TELEOST CYPRINUS CARPIO: TISSUE DISTRIBUTION,POSSIBLE INVOLVEMENT IN PROLACTIN PROCESSING AND EFFECT OF 17 B-ESTRADIOL IN VIVO - (Peer Reviewed Journal) - (31-May-02)

DETECTION OF BOTH HEPATITIS A VIRUS AND NORWALK-LIKE VIRUS IN IMPORTED CLAMS ASSOCIATED WITH FOOD-BORNE ILLNESS - (Peer Reviewed Journal) - (01-May-02)

INACTIVATION OF HEPATITIS A AND A CALICIVIRUS BY HIGH HYDROSTATIC PRESSURE - (Peer Reviewed Journal) - (25-Apr-02)

THE EVOLUTION OF MOLLUSCAN SHELLFISH SAFETY - (Proceedings)

RICHARDS, G.P. THE EVOLUTION OF MOLLUSCAN SHELLFISH SAFETY. MEETING ABSTRACT. 2002. p.78