Projects

Molecular analysis of effects of environment on wheat flour quality and allergenic potential Appropriated (D)   Accession Number: 419972

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Comparative proteomic analysis of the effect of temperature and fertilizer on gliadin and glutenin accumulation in the developing endosperm and flour from Triticum aestivum L. cv. Butte 86. - (Peer Reviewed Journal)

Hurkman Ii, W.J., Tanaka, C.K., Vensel, W.H., Thilmony, R.L., Altenbach, S.B. 2013. Comparative proteomic analysis of the effect of temperature and fertilizer on gliadin and glutenin accumulation in the developing endosperm and flour from Triticum aestivum L. cv. Butte 86.. Proteome Science. doi:10.1186/1477-5956-11-8.

Variant high-molecular-weight glutenin subunits arising from biolistic transformation of wheat - (Peer Reviewed Journal)

Blechl, A.E., Vensel, W.H. 2013. Variant high-molecular-weight glutenin subunits arising from biolistic transformation of wheat. Journal of Cereal Science. 57:496-503.

Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE - (Peer Reviewed Journal)

Altenbach, S.B., Tanaka, C.K., Hurkman Ii, W.J., Whitehand, L.C., Vensel, W.H., Dupont, F.M. 2011. Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE. Proteome Science. 9:46.

The spectrum of low molecular weight alpha-amylase/protease inhibitor genes expressed in the US bread wheat Butte 86 - (Peer Reviewed Journal)

Altenbach, S.B., Vensel, W.H., Dupont, F.M. 2011. The spectrum of low molecular weight alpha-amylase/protease inhibitor genes expressed in the US bread wheat Butte 86. BMC Research Notes. 4:242.

Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry - (Peer Reviewed Journal)

Dupont, F.M., Vensel, W.H., Tanaka, C.K., Hurkman II, W.J., Altenbach, S.B. 2011. Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry. Proteome Science. 9(10) available:http://www.proteomesci.com/content/9/1/10.

Effect of cleavage enzyme, search algorithm and decoy database on mass spectrometric identification of wheat gluten proteins - (Peer Reviewed Journal)

Vensel, W.H., Dupont, F.M., Altenbach, S.B., Sloane, S.M. 2011. Effect of Cleavage Enzyme, Search Algorithm and Decoy Database on Mass Spectrometric Identification of Wheat Gluten Proteins. Phytochemistry. 72(10):1154-1161.

Integration of transcriptomic and proteomic data from a single wheat cultivar provides new tools for understanding the roles of individual alpha gliadin proteins in flour quality and celiac disease - (Peer Reviewed Journal)

Altenbach, S.B., Vensel, W.H., Dupont, F.M. 2010. Integration of transcriptomic and proteomic data from a single wheat cultivar provides new tools for understanding the roles of individual alpha gliadin proteins in flour quality and celiac disease. Journal of Cereal Science. 52:143-151.

Assessing the Role of Oxidized Methionine at Position 213 in the Formation of Prions in Hamsters - (Peer Reviewed Journal)

Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson, M.L., Vensel, W.H., Requena, J., Antaki, E.M., Carter, J.M. 2010. Assessing the Role of Oxidized Methionine at Position 213 in the Formation of Prions in Hamsters. Journal of Biochemistry. (49):1854-1861.

Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that may play different functional roles in flour - (Peer Reviewed Journal)

Altenbach, S.B., Vensel, W.H., Dupont, F.M. 2010. Analysis of expressed sequence tags from a single wheat cultivar facilitates interpretation of tandem mass spectrometry data and discrimination of gamma gliadin proteins that may play different functional roles in flour. Biomed Central (BMC) Plant Biology. 10(7):1471-2229. Available: http://www.biomedcentral.com/1471-2229/10/7.

Analysis of ESTs from the US wheat Butte 86 Facilitates MS/MS Differentiation of Alpha and Gamma Gliadin Proteins with Very Similar Sequences - (Proceedings)

Altenbach, S.B., Vensel, W.H., Dupont, F.M. 2010. Analysis of ESTs from the US wheat Butte 86 Facilitates MS/MS Differentiation of Alpha and Gamma Gliadin Proteins with Very Similar Sequences. International Gluten Workshop. p.242-246.

Differential effects of mineral nutrition on wheat flour proteins determined by quantitative 2-DE and MS/MS - (Proceedings)

Dupont, F.M., Altenbach, S.B., Vensel, W.H., Tanaka, C.K., Hurkman II, W.J. 2010. Differential effects of mineral nutrition on wheat flour proteins determined by quantitative 2-DE and MS/MS. International Gluten Workshop. p.64-67.

The effect of high temperature on cell structure and gluten protein accumulation in the endosperm of the developing wheat (Triticum aestivum L.) grain - (Proceedings)

Hurkman Ii, W.J., Tanaka, C.K., Thilmony, R.L., Williams, T.G., Wood, D.F., Vensel, W.H., Dupont, F.M., Altenbach, S.B. 2010. The effect of high temperature on cell structure and gluten protein accumulation in the endosperm of the developing wheat (Triticum aestivum L.) grain. International Gluten Workshop. pp. 50-54.

Differential effects of mineral nutrition on wheat flour proteins determined by quantitative 2-DE and MS/MS - (Abstract Only)

Dupont, F.M., Altenbach, S.B., Vensel, W.H., Tanaka, C.K., Hurkman Ii, W.J. 2010. Differential effects of mineral nutrition on wheat flour proteins determined by quantitative 2-DE and MS/MS. Xth international Gluten Workshop, Clermont-Ferrand, France, September 7-9, 2009.

Effect of high temperature on cell structure and gluten protein accumulation in the endosperm of the developing wheat (Triticum aestivum L.) grain - (Abstract Only)

Hurkman Ii, W.J., Tanaka, C.K., Wood, D.F., Vensel, W.H., Altenbach, S.B., Dupont, F.M. 2010. Effect of high temperature on cell structure and gluten protein accumulation in the endosperm of the developing wheat (Triticum aestivum L.) grain. International Gluten Workshop. p. 50.

Applying Genetic Engineering to Understand the Contributions of Various Seed Proteins to Wheat Flour Properties - (Abstract Only)

Blechl, A.E., Lin, J.W., Altenbach, S.B., Hurkman Ii, W.J., Sloane, S.M., Tanaka, C.K., Vensel, W.H. 2010. Applying Genetic Engineering to Understand the Contributions of Various Seed Proteins to Wheat Flour Properties. Xth International Gluten Workshop, September 7-9, 2009, Clermont-Ferrand, France.

Mass Spectrometric Identification of the Arginine and Lysine deficient Proline Rich Glutamine Rich Wheat Storage Proteins - (Abstract Only) - (12-Jan-09)

Thioredoxin targets in plants: The first 30 years - (Peer Reviewed Journal)

Montrichard, F., Alkhalfioui, F., Yano, H., Vensel, W.H., Hurkman II, W.J., Buchanan, B.B. 2009. Thioredoxin targets in plants: The first 30 years. Journal of Proteomics. 72(3):452-474.

The surface-associated proteins of wheat starch granules: suitability of wheat starch for celiac patients - (Peer Reviewed Journal)

Kasarda, D.D., Dupont, F.M., Vensel, W.H., Altenbach, S.B., Lopez, R., Tanaka, C.K., Hurkman II, W.J. 2008. The surface-associated proteins of wheat starch granules: suitability of wheat starch for celiac patients. Journal of Agricultural and Food Chemistry. 56:10292-10302.

Effect of High Temperature on Albumin and Globulin Accumulation in the Endosperm Proteome of the Developing Wheat Grain - (Peer Reviewed Journal)

Hurkman II, W.J., Vensel, W.H., Tanaka, C.K., Whitehand, L.C., Altenbach, S.B. 2009. Effect of High Temperature on Albumin and Globulin Accumulation in the Endosperm Proteome of the Developing Wheat Grain. Journal of Cereal Science. 49:12-23.

Expression of globulin-2, a member of the cupin superfamily of proteins with similarity to known food allergens, is increased under high temperature regimen during wheat grain development - (Peer Reviewed Journal)

Altenbach, S.B., Tanaka, C.K., Hurkman II, W.J., Vensel, W.H. 2009. Expression of globulin-2, a member of the cupin superfamily of proteins with similarity to known food allergens, is increased under high temperature regimen during wheat grain development. Journal of Cereal Science. 49:47-54.

Digestibility of Protein and Starch from Sorghum (Sorghum bicolor)is Linked to Biochemical and Structural Features of Grain Endosperm - (Peer Reviewed Journal)

Wong, J.H., Lau, T., Cai, N., Singh, J., Pedersen, J.F., Vensel, W.H., Hurkman II, W.J., Wilson, J.D., Lemaux, P.G., Buchanan, B.B. 2008. Digestibility of Protein and Starch from Sorghum (Sorghum bicolor)is Linked to Biochemical and Structural Features of Grain Endosperm. Journal of Cereal Science. 49(1):73-82.

Globulins Are the Main Seed Storage Proteins in Brachypodium Distachyon - (Peer Reviewed Journal)

Chingcuanco, D.L., Vensel, W.H. 2008. Globulins are the main seed storage proteins in brachypodium distachyon. Theoretical and Applied Genetics. 117:555-563.

Comparative transcriptional and proteomic profiling of bread wheat cultivar and its derived transgenic line over-expressing a low molecular weight glutenin subunit gene in the endosperm - (Peer Reviewed Journal)

Scossa, F., Chingcuanco, D.L., Anderson, O.D., Vensel, W.H., Lafiandra, D., D'Ovidio, R., Masci, S. 2008. Comparative transcriptional and proteomic profiling of bread wheat cultivar and its derived transgenic line over-expressing a low molecular weight glutenin subunit gene in the endosperm. Molecular and Cellular Proteomics. 8(14):2948-2966.

Effect of High Temperature on Albumin and Globulin Accumulation in the Endosperm Proteome of the Developing Wheat Grain - (Abstract Only)

Vensel, W.H., Hurkman II, W.J., Tanaka, C.K., Whitehand, L.C., Altenbach, S.B. 2008. Effect of High Temperature on Albumin and Globulin Accumulation in the Endosperm Proteome of the Developing Wheat Grain. Association of Biomolecular Resource Facilities, 2008 Annual Meeting, February 9-12, 2008, Salt Lake City, Utah.

Sensitive, Preclinical Detection of Prions in Brain by nanospray liquid chromatography/tandem mass spectrometry - (Peer Reviewed Journal)

Onisko, B.C., Silva, C.J., Dynin, I.A., Erickson, M.L., Vensel, W.H., Hnasko, R.M., Requena, J.R., Carter, J.M. 2007. Sensitive, Preclinical Detection of Prions in Brain by nanospray liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry. 21(24):4023-4026.

Alterations in protein expression associated with the development of mealiness in peaches - (Peer Reviewed Journal)

Obenland, D.M., Vensel, W.H., Hurkman, W.J. 2008. Alterations in protein expression associated with the development of mealiness in peaches. Journal of Horticultural Science and Biotechnology. 83(1):85-93.

Effects of Mineral Nutrition and Temperature on Accumulation of Gluten Proteins are Related to Their Content of CYs and Met - (Book / Chapter)

Dupont, F.M., Hurkman, W.J., Vensel, W.H., Chan, R., Tanaka, C.K., Altenbach, S.B. 2007. Effects of Mineral Nutrition and Temperature on Accumulation of Gluten Proteins are Related to Their Content of CYs and Met. In: George L. Lookhart, Perry K. W. Ng, editors. Gluten Proteins 2006. Minneapolis, MN: AACCI. p. 38-42.

Thioredoxin-linked proteins are reduced during germination of medicago truncatula seeds - (Peer Reviewed Journal)

Alkhalfiour, F., Renard, M., Wong, J., Tanaka, C.K., Vensel, W.H., Hurkman, W.J., Buchanan, B.B., Montichard, F. 2007. Thioredoxin-linked proteins are reduced during germination of medicago truncatula seeds. Plant Physiology. 144: 1559-1579.

Mass Spectrometry Based Identifications of LMW Glutenin Subunits - (Book / Chapter)

Vensel, W.H., Dupont, F.M., Chan, R., Hurkman, W.J. 2007. Mass Spectrometry Based Identifications of LMW Glutenin Subunits. In: George L. Lookhart, Perry K.W. Ng, editors. Gluten Proteins 2006. Minneapolis, MN: AACCI. P. 347-351.

Expression Patterns in Transgenic Wheats with Elevated Levels of Dx5 and/or Dy10 Glutenin Subunits - (Book / Chapter)

Blechl, A.E., Lin, J.W., Nguyen, S., Dupont, F.M., Vensel, W.H., Chan, R., Anderson, O.D., Bregitzer, P.P., Fiedler, D.J. 2007. Expression Patterns in Transgenic Wheats with Elevated Levels of Dx5 and/or Dy10 Glutenin Subunits. In: George L. Lookhart, Perry K.W. Ng, editors. Gluten Proteins 2006. Minneapolis, MN: AACCI. p. 107-111.

Endosperm and Amyloplast Proteomes of Wheat Grain - (Book / Chapter)

Hurkman II, W.J., Vensel, W.H., Dupont, F.M., Altenbach, S.B., Buchanan, B.B. 2008. Endosperm and Amyloplast Proteomes of Wheat Grain. In: Agrawal, G.K. and Rakwal, R., editors. Plant Proteomics: Technologies, Strategies, and Applictions. John Wiley and Sons, Inc., Hoboken, NJ. p. 207-222.

Biochemical and Genetic Characterization of Wheat (Triticum SPP.) Kernel Polyphenol Oxidases - (Peer Reviewed Journal)

Anderson, J.V., Fuerst, E.P., Hurkman, W.J., Vensel, W.H., Morris, C.F. 2006. Biochemical and genetic characterization of wheat (Triticum spp.) kernel polyphenol oxidases. Journal of Cereal Science. 44(3):353-367.

Differential Accumulation of Sulfur-rich and Sulfur-poor Wheat Flour Proteins is Affected by Temperature and Mineral Nutrition during Grain Development - (Peer Reviewed Journal)

Dupont, F.M., Hurkman, W.J., Vensel, W.H., Chan, R., Lopez, R., Tanaka, C.K., Altenbach, S.B. 2006. Accumulation of sulfur-rich and sulfur-poor wheat flour proteins are affected by temperature and mineral nutrition during grain development. Journal of Cereal Science. 44(1): 101-112

Proteome of Amyloplasts Isolated from Developing Wheat Endosperm Presents Evidence of Broad Metabolic Capability - (Peer Reviewed Journal)

Balmer, Y., Vensel, W.H., Dupont, F.M., Buchanan, B., Hurkman, W.J. 2006. Proteome of amyloplasts isolated developing wheat endosperm presents evidence of broad metabolic capability. Journal of Experimental Botany. 57(7): 1591-1602

A Complete Ferredoxin/thioredoxin System Regulates Fundamental Processes in Amyloplasts - (Peer Reviewed Journal)

Balmer, Y., Vensel, W.H., Cai, N., Manieri, W., Schurmann, P., Hurkman, W.J., Buchanan, B. 2006. A complete ferredoxin/thioredoxin system regulates fundamental processes in amyloplasts. Proceedings of the National Academy of Sciences. 103(8): 2988-2993

Protein Accumulation and Composition in Wheat Grains: Effects of Mineral Nutrients and High Temperature - (Peer Reviewed Journal)

Dupont, F.M., Hurkman, W.J., Vensel, W.H., Tanaka, C., Kothari, K.M., Chung, O.K., Altenbach, S.B. 2006. Protein accumulation and composition in wheat grains: Effects of mineral nutrients and high temperature. 25(2):96-107.

Genomic and Proteomic Identification of a Dna-Binding Protein Used in the "fingerprinting" of Campylobacter Species and Strains by Maldi-Tof-Ms Protein Biomarker Analysis - (Peer Reviewed Journal)

Fagerquist, C.K., Miller, W.G., Harden, L.A., Bates, A.H., Vensel, W.H., Wang, G., Mandrell, R.E. 2005. Genomic and proteomic identification of a dna-binding protein used in the "fingerprinting" of campylobacter species and strains by maldi-tof-ms protein biomarker analysis. Analytical Chemistry. web release 6/25/05, 77:4897-4907

Proteome Mapping of Mature Pollen of Arabidopsis Thaliana - (Peer Reviewed Journal)

Holmes-Davis, R.A., Tanaka, C.K., Vensel, W.H., Hurkman, W.J., McCormick, S. 2005. Proteome mapping of mature pollen of Arabidopsis thaliana. Proteomics. 5(18):4864-84.

Biosynthesis of Gliadins and Glutenin Subunits During Grain Development under Different Environmental Conditions - (Proceedings)

Altenbach, S.B., Dupont, F.M., Hurkman, W.J., Vensel, W.H. 2005. Biosynthesis of gliadins and glutenin subunits during grain development under different environmental conditions. In: D.K. Chung and G.L. Lockhart), editors. Proceedings of the Third International Wheat Quality Conference., May 22-26, 2005, P. 189-194.

Sequential Extraction and Quantitative Recovery of Gliadins, Glutenins and Other Proteins from Small Samples of Wheat Flour - (Peer Reviewed Journal)

Dupont, F.M., Chan, R., Lopez, R., Vensel, W.H. 2005. Sequential extraction and quantitative recovery of gliadins, glutenins and other proteins from small samples of wheat flour. Journal of Agricultural and Food Chemistry. 53(5):1575-1584.

Developmental Changes in the Metabolic Protein Profiles of Wheat Endosperm - (Peer Reviewed Journal)

Vensel, W.H., Tanaka, C.K., Cal, N., Wong, J.H., Buchanan, B.B., Hurkman, W.J. 2005. Developmental changes in the metabolic protein profiles of wheat endosperm. Encyclopedia of Diagnostic Genomics and Proteomics.78:53-61

Thioredoxin Targets of Developing Wheat Seeds Identified by Complementary Proteomics Approaches. - (Other)

Wong, J.H., Cai, N., Balmer, Y., Tanaka, C.K., Vensel, W.H., Hurkman, W.J., Buchanan, B.B. 2004. Thioredoxin targets of developing wheat seeds identified by complementary proteomics approaches.. Phytochemistry. 65:1629-1640

Tioredoxin Reduction Alters the Solubility of Proteins of Wheat Starchy Endosperm: An Early Event in Cereal Germination. - (Peer Reviewed Journal)

Wong, J.H., Cai, N., Tanaka, C.K., Vensel, W.H., Hurkman, W.J., Buchanan, B.B. 2004. Tioredoxin reduction alters the solubility of proteins of wheat starchy endosperm: an early event in cereal germination.. Plant And Cell Physiology.2004. 45: 407-415

Thioredoxin Links Redox to the Regulation of Fundamental Processes of Plant Mitochondria - (Peer Reviewed Journal)

Balmer, Y., Vensel, W.H., Tanaka, C.K., Hurkman, W.J., Gelhaye, E., Rouhier, N., Hacquot, J., Manieri, W., Schurmann, P., Buchanan, B.B. 2004. Thioredoxin links redox to the regulation of fundamental processes of plant mitochondria. Proceedings of the National Academy of Sciences. 2004. 101: 2642-2647

Similarities of Omega Gliadins from Triticum Urartu to Those Encoded on Chromosome 1a of Hexaploid Wheat and Evidence for Their Post-Translational Processing - (Peer Reviewed Journal)

Dupont, F.M., Encarnacao, T.M., Chan, R., Vensel, W.H., Kasarda, D.D. 2004. Identification and characterization of omega gliadins from t. urartu and those encoded on chromosome 1a of hexaploid wheat. Theoretical and Applied Genetics. 108:1299-1308.

Analysis of Wheat Endosperm Proteins During Grain Development and in Response to High Temperature Using Proteomics - (Abstract Only)

Hurkman, W.J., Tanaka, C.K., Vensel, W.H., Wong, J.H., Balmer, Y., Buchanan, B.B. 2004. Analysis of wheat endosperm proteins during grain development and in response to high temperature using proteomics. International Gluten Workshop. p. 169-172

Characterization of Omega Gliadins Encoded on Chromosme 1a and Evidence for Post-Translational Cleavage of Omega Gliadins by An Asparaginyl Endoprotease - (Proceedings)

Dupont, F.M., Vensel, W.H., Kasarda, D.D. 2003. Characterization of omega gliadins encoded on chromosme 1a and evidence for post-translational cleavage of omega gliadins by an asparaginyl endoprotease. Wheat Genetics International Symposium Proceedings. 3: 946-948

Unraveling Thioredoxin-Linked Metabolic Processes of Cereal Starchy Endosperm Using Proteomics - (Peer Reviewed Journal)

Wong, J.H., Balmer, Y., Cai, N., Tanaka, C.K., Vensel, W.H., Hurkman, W.J., Buchanan, B.B. 2003. Unraveling thioredoxin-linked metabolic processes of cereal starchy endosperm using proteomics. Federation of European Biochemical Societies Letters.2003. 547: 151-156

Characterization of Omega Gliadins Encoded on Chromosome 1a and Evidence for Post-Translational Cleavage of Omega Gliadins by An Asparaginyl Endoprotease - (Proceedings)

DUPONT, F.M., VENSEL, W.H., KASARDA, D.D., FIEDLER, D.J. 2003. CHARACTERIZATION OF OMEGA GLIADINS ENCODED ON CHROMOSOME 1A AND EVIDENCE FOR POST-TRANSLATIONAL CLEAVAGE OF OMEGA GLIADINS BY AN ASPARAGINYL ENDOPROTEASE. 8th International Gluten Workshop, Viterbo, Italy. p. 42-45.

Genomics, Proteomics and Cereal Chemistry: a Combined Approach for Discovering the Effects of Environment on Wheat Grain Development and Flour Quality - (Abstract Only)

ALTENBACH, S.B., DUPONT, F.M., HURKMAN, W.J., VENSEL, W.H. GENOMICS, PROTEOMICS AND CEREAL CHEMISTRY: A COMBINED APPROACH FOR DISCOVERING THE EFFECTS OF ENVIRONMENT ON WHEAT GRAIN DEVELOPMENT AND FLOUR QUALITY. Meeting Abstract. 2003.