Projects

Molecular Tools to Minimize Risk in Genetically Engineered Crops Appropriated (D)   Accession Number: 419129

PRODUCTION OF WHEAT GERMPLASM WITH ENHANCED BAKING QUALITY Appropriated (D)   Accession Number: 419970

Developing and Deploying New Tools for Crop Plant Biotechnology Specific Cooperative Agreement (S)   Accession Number: 421372

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Effects of transgene-encoded high-molecular weight glutenin proteins in wheat flour blends and sponge and dough baking - (Peer Reviewed Journal)

Graybosch, R.A., Seabourn, B.W., Chen, Y.R., Blechl, A.E. 2013. Effects of transgene-encoded high-molecular weight glutenin proteins in wheat flour blends and sponge and dough baking. Cereal Chemistry. 90(02): 164-168.

Physical characteristics of genetically-altered wheat related to technological protein separation - (Peer Reviewed Journal)

Robertson, G.H., Blechl, A.E., Hurkman Ii, W.J., Anderson, O.D., Cao, T., Tanaka, C.K., Gregorski, K.S., Orts, W.J. 2013. Physical characteristics of genetically-altered wheat related to technological protein separation . Cereal Chemistry. 90(1): 1-12.

Bxb1 recombinase mediates site-specific deletions in transgenic wheat - (Peer Reviewed Journal)

Blechl, A.E., Lin, J.W., Shao, M., Thilmony, R.L., Thomson, J.G. 2012. Bxb1 recombinase mediates site-specific deletions in transgenic wheat. Plant Molecular Biology Reporter. doi:10.1007/s11105-012-0454-2.

Quality and agronomic effects of three high-molecular-weight glutenin subunit transgenic events in winter wheat - (Peer Reviewed Journal)

Graybosch, R.A., Seabourn, B.W., Chen, Y.R., Blechl, A.E. 2010. Quality and agronomic effects of three high-molecular-weight glutenin subunit transgenic events in winter wheat. Cereal Chemistry. 88(1):95-102.

Expression of a truncated form of yeast ribosomal protein L3 in transgenic wheat improves resistance to Fusarium head blight - (Peer Reviewed Journal)

Di, R., Blechl, A.E., Dill-Macky, R., Tortora, A., Tumer, N.E. 2010. Expression of a truncated form of yeast ribosomal protein L3 in transgenic wheat improves resistance to Fusarium head blight. Plant Science. 178: 374-380.

Applying Genetic Engineering to Understand the Contributions of Various Seed Proteins to Wheat Flour Properties - (Abstract Only)

Blechl, A.E., Lin, J.W., Altenbach, S.B., Hurkman Ii, W.J., Sloane, S.M., Tanaka, C.K., Vensel, W.H. 2010. Applying Genetic Engineering to Understand the Contributions of Various Seed Proteins to Wheat Flour Properties. Xth International Gluten Workshop, September 7-9, 2009, Clermont-Ferrand, France.

EFFECTS OF OVER-EXPRESSION OF HIGH MOLECULAR WEIGHT GLUTENIN SUBUNIT 1Dy10 ON WHEAT TORTILLA PROPERTIES - (Peer Reviewed Journal)

Pierucci, V.R., Tilley, M., Graybosch, R.A., Blechl, A.E., Bean, S., Tilley, K.A. 2009. Effects of Overexpression of High Molecular Weight Glutenen Subunit 1Dy10 on Wheat Tortilla Properties. Journal of Agricultural and Food Chemistry. 57:6318-6326.

Wheat: Science and Trade - (Book / Chapter)

Blechl, A.E., Jones, H.D. 2009. "Transgenic Applications in Wheat Improvement" In: Carver, Brett F, editor. Wheat: Science and Trade. Wiley-blackwell, Edison, NJ. p. 397-435.

Intragenic Applications for Wheat Genetic Engineering - (Abstract Only)

Blechl, A.E. 2009. Intragenic Applications for Wheat Genetic Engineering (abstract). Crop Improvement and Utilization Intragenics Workshop. p. 6.

Positional Cloning of a QTL for slow rusting in wheat - (Abstract Only)

Dubcovsky, J., Fu, D., Uauy, C., Blechl, A.E., Epstein, L., Chen, X., Distelfeld, A., Sela, H., Fahima, T. 2009. Positional Cloning of a QTL for slow rusting in wheat. [online only]. Available: http://www.intl-pag.org/17/abstracts/W61-PAGXVII-426.html.

A kinase-START gene confers temperature-dependent resistance to wheat stripe rust - (Peer Reviewed Journal)

Fu, D., Uauy, C., Distelfeld, A., Blechl, A.E., Epstein, L., Chen, X., Sela, H., Fahima, T., Dubcovsky, J. 2009. A kinase-START gene confers temperature-dependent resistance to wheat stripe rust. Science. 323:1357-1360.

Stably Expressed D Genome-derived HMW Glutenin Subunit Genes Transformed Into Different Durum Wheat Genotypes Change Dough Mixing Properties - (Peer Reviewed Journal)

Gadaleta, A., Blechl, A.E., Nguyen, S.B., Cardone, M.F., Ventura, M., Quick, J.S., Blanco, A. 2008. Stably Expressed D Genome-derived HMW Glutenin Subunit Genes Transformed Into Different Durum Wheat Genotypes Change Dough Mixing Properties. Molecular Breeding. 22:267-279.

A Transgenic Durum Wheat Line that is Free of Marker Genes and Expresses 1dy10 - (Peer Reviewed Journal)

Gadaleta, A., Giancaspro, A., Blechl, A.E., Blanco, A. 2008. A Transgenic Durum Wheat Line that is Free of Marker Genes and Expresses 1dy10. Journal of Cereal Science. 48:439-445.

THE EXPRESSION OF A BEAN PGIP IN TRANSGENIC WHEAT CONFERS INCREASED RESISTANCE TO THE FUNGAL PATHOGEN BIPOLARIS SOROKINIANA - (Peer Reviewed Journal)

Janni, M., Sella, L., Favaron, F., Blechl, A.E., De Lorenzo, G., D'Ovidio, R. 2008. The expression of a bean pgip in transgenic wheat confers increased resistance to the fungal pathogen bipolaris sorokiniana. Molecular Plant-Microbe Interactions.21:171-177.

RNA Interference for Wheat Functional Gene Analysis - (Peer Reviewed Journal)

Fu, D., Uauy, C., Blechl, A.E., Dubcovsky, J. 2007. RNA interference for wheat functional gene analysis. Transgenic Research. 16:689-701.

Expression Patterns in Transgenic Wheats with Elevated Levels of Dx5 and/or Dy10 Glutenin Subunits - (Book / Chapter)

Blechl, A.E., Lin, J.W., Nguyen, S., Dupont, F.M., Vensel, W.H., Chan, R., Anderson, O.D., Bregitzer, P.P., Fiedler, D.J. 2007. Expression Patterns in Transgenic Wheats with Elevated Levels of Dx5 and/or Dy10 Glutenin Subunits. In: George L. Lookhart, Perry K.W. Ng, editors. Gluten Proteins 2006. Minneapolis, MN: AACCI. p. 107-111.

Expression of the N-terminal 99 Amino Acids of Yeast Ribosomal Protein L3 in Transgenic Wheat Confers Resistance to Fusarium Head Blight - (Abstract Only)

Di, R., Blechl, A.E., Dill-Macky, R., Tortora, A., Tumer, N.E. 2007. Expression of the N-terminal 99 Amino Acids of Yeast Ribosomal Protein L3 in Transgenic Wheat Confers Resistance to Fusarium Head Blight. The 2nd International Conference on Plant Molecular Biology, March 23-27, 2007, Sanya, China. Molecular Plant Breeding. 5:283.

The TRI101 Story: Engineering Wheat and Barley to Resist Fusarium Head Blight - (Abstract Only)

Alexander, N.J., Blechl, A.E., Okubara, P.A., Mc Cormick, S.P., Manoharan, M., Dahleen, L.S., Kendra, D.F. 2006. The TRI101 Story: Engineering Wheat and Barley to Resist Fusarium Head Blight [abstract]. World Mycotoxin Forum, the Fourth Conference. p. 123.

The wheat and barley vernalization gene VRN3 is an orthologue of FT - (Peer Reviewed Journal)

Yan, L., Fu, D., Li, C., Blechl, A.E., Tranquilli, G., Bonafede, M., Sanchez, A.N., Valarik, M., Yasuda, S., Dubcovsky, J. 2006. The wheat and barley vernalization gene VRN3 is an orthologue of FT. Proceedings of the National Academy of Sciences. 103: 19581-19586

A NAC Gene Regulating Senescence Improves Grain Protein, Zinc, and Iron Content in Wheat - (Peer Reviewed Journal)

Uauy, C., Distelfeld, A., Fahima, T., Blechl, A.E., Dubcovsky, J. 2006. A NAC gene regulating senescence improves grain protein, zinc, and iron content in wheat. Science. 314(5803):1298-1301.

Expression patterns in transgenic wheats with elevated levels of Dx5 and/or Dy10 glutenin subunits - (Proceedings)

Blechl, A.E., Lin, J.W. 2007. Expression patterns in transgenic wheats with elevated levels of Dx5 and/or Dy10 glutenin subunits. International Gluten Workshop. Gluten Proteins 2006, pg. 107-111. tb

COMPLEMENTATION OF THE PINA (NULL) ALLELE WITH THE WILD TYPE PINA SEQUENCE RESTORES A SOFT PHENOTYPE IN TRANSGENIC WHEAT - (Peer Reviewed Journal)

Martin, J.M., Meyer, F.D., Smidansky, E.D., Wanjugi, H., Blechl, A.E., Giroux, M.J. 2006. Complementation of the pina (null) allele with the wild type pina sequence restores a soft phenotype in transgenic wheat. Theoretical and Applied Genetics. 113(8):1563-1570.

TRANSGENIC WHEATS WITH ELEVATED LEVELS OF DX5 AND/OR DY10 HIGH-MOLECULAR-WEIGHT GLUTENIN SUBUNITS YIELD DOUGHS WITH INCREASED MIXING STRENGTH AND TOLERANCE - (Peer Reviewed Journal)

Blechl, A.E., Lin, J.W., Nguyen, S., Chan, R., Anderson, O.D., Dupont, F.M. 2007. Transgenic wheats with elevated levels of dx5 and/or dy10 high-molecular-weight glutenin subunits yield doughs with increased mixing strength and tolerance. Journal of Cereal Science. 45:172-183

PGPRO 1, A NOVEL BINARY VECTOR FOR MONOCOT PROMOTER CHARACTERIZATION - (Peer Reviewed Journal)

Thilmony, R.L., Guttman, M.E., Chiniquy, D., Blechl, A.E. 2006. Pgpro 1, a novel binary vector for monocot promoter characterization. Plant Molecular Biology Reporter. 24:1-13, March 2006

CHANGES IN HIGH MOLECULAR WEIGHT GLUTENIN SUBUNIT COMPOSITION CAN BE GENETICALLY ENGINEERED WITHOUT IMPACTING WHEAT AGRONOMIC PERFORMANCE - (Peer Reviewed Journal)

Bregitzer, P.P., Fiedler, D.J., Blechl, A.E., Lin, J.W., Sebesta, P.G., Fernandez De Soto, J., Chicaiza, O., Dubcovsky, J. 2006. Changes in high molecular weight glutenin subunit composition can be genetically engineered without impacting wheat agronomic performance. Crop Science. 46:1553-1563 (2006)

ACCUMULATION OF TRANSGENE-ENCODED DEFENSE-ASSOCIATED ENZYMES IN TISSUES VULNERABLE TO INITIAL FUSARIUM INFECTION - (Abstract Only)

Blechl, A.E., Somleva, M.N. Accumulation of transgene-encoded defense-associated enzymes in tissues vulnerable to initial fusarium infection. National Fusarium Head Blight Forum Proceedings. pp. 102

PHOSPHOMANNOSE ISOMERASE, PMI, AS A SELECTABLE MARKER GENE FOR DURUM WHEAT TRANSFORMATION - (Peer Reviewed Journal)

Gadaleta, A., Giancaspro, A., Blechl, A.E., Blanco, A. 2006. Phosphomannose isomerase, pmi, as a selectable marker gene for durum wheat transformation. Journal of Cereal Science. 43: 31-37

REGULATION OF VRN-1 VERNALIZATION GENES IN NORMAL AND TRANSGENIC POLYPLOID WHEAT - (Peer Reviewed Journal)

Loukoinov, A., Yan, L., Blechl, A.E., Sanchez, A., Dubcovsky, J. 2005. Regulation of vrn-1 vernalization genes in normal and transgenic polyploid wheat. Journal of Plant Physiology. Vol. 138, P. 1-10.

NEW APPROACHES TO OVERCOME FUSARIUM HEAD BLIGHT - (Proceedings)

Blechl, A.E., Somleva, M.N., Okubara, P.A., Dill-Mackey, R. 2005. New approaches to overcome fusarium head blight. International Wheat Quality Conference. p. 85-95.

ATOMIC FORCE MICROSCOPY OF A HYBRID HIGH-MOLECULAR-WEIGHT GLUTENIN SUBUNIT FROM A TRANSGENIC HEXAPLOID WHEAT - (Peer Reviewed Journal)

McIntire, T.M., Lew, E.J.L., Adalsteins, A.E., Blechl, A., Anderson, O.D., Brant, D.A., Kasarda, D.D. 2005. Atomic force microscopy of a hybrid high-molecular-weight glutenin subunit from a transgenic hexaploid wheat. Biopolymers. 78(2):53-61.

CHARACTERIZATION OF ORGAN SPECIFIC PROMOTERS IN TRANSGENIC WHEAT - (Proceedings)

Somleva, M.N., Blechl, A.E. 2004. Characterization of organ specific promoters in transgenic wheat. National Fusarium Head Blight Forum Proceedings. P. 263-267

AGRONOMIC, BIOCHEMICAL AND QUALITY CHARACTERISTICS OF WHEATS CONTAINING HMW-GLUTENIN TRANSGENES - (Proceedings)

Blechl, A.E., Bregitzer, P.P., O'Brien, K., Lin, J.W., Nguyen, S.B., Anderson, O.D. 2004. Agronomic, biochemical and quality characteristics of wheats containing hmw-glutenin transgenes. Proceedings - 8th Gluten Workshop. p. 6-9.

CHARACTERIZATION OF GLUTENIN POLYMERS IN A TRANSGENIC BREAD WHEAT LINE OVER-EXPRESSING A LMW-GS. - (Proceedings)

Masci, S., D'Ovidio, R., Scossa, F., Patacchini, C., Lafiandra, D., Anderson, O.D., Blechl, A.E. 2004. Characterization of glutenin polymers in a transgenic bread wheat line over-expressing a LNW-GS. Proceedings - 8th Gluten Workshop. 1:10-13

THE WHEAT VRN2 GENE IS A FLOWERING REPRESSOR DOWNREGULATED BY VERNALIZATION - (Peer Reviewed Journal)

Yan, L., Loukoinov, A., Blechl, A.E., Tranquilli, G., Ramakrishna, W., Sanmiguel, P., Bennetzen, J.L., Echenique, V., Dubcovsky, J. 2004. The wheat vrn2 gene is a flowering repressor downregulated by vernalization. Science. 303: 1640-1644

PRODUCTION AND CHARACTERIZATION OF A TRANSGENIC BREAD WHEAT LINE OVER-EXPRESSING A LOW-MOLECULAR WEIGHT GLUTENIN SUBUNIT GENE. - (Peer Reviewed Journal)

MASCI, S., D'OVIDIO, R., SCOSSA, F., PATACCHINI, C., LAFIANDRA, D., ANDERSON, O.D., BLECHL, A.E. PRODUCTION AND CHARACTERIZATION OF A TRANSGENIC BREAD WHEAT LINE OVER-EXPRESSING A LOW-MOLECULAR WEIGHT GLUTENIN SUBUNIT GENE. MOLECULAR BREEDING. 2003. v. 12. p. 209-222.

TRANSGENE EXPRESSION IN SPRING WHEAT (TRITICUM AESTIVUM L.) TRANSFORMED WITH CANDIDATE ANTI-FUSARIUM GENES - (Proceedings)

Somleva, M., Okubara, P.A., Blechl, A.E. 2002. Transgene expression in spring wheat (triticum aestivum l.) transformed with candidate anti-fusarium genes. National Fusarium Head Blight Forum Proceedings. 42-45

ENGINEERING DEOXYNIVALENOL METABOLISM IN WHEAT THROUGH THE EXPRESSION OF A FUGAL TRICHOTHECENE ACETYLTRANSFERASE GENE - (Peer Reviewed Journal) - (05-Apr-02)

EXPRESSION OF TWO DIFFERENT CANDIDATE ANTI-FUSARIUM PROTEIN GENES AFFORDS PARTIAL PROTECTION AGAINST THE SPREAD OF FUSARIUM GRAMINEARUM - (Abstract Only) - (30-Nov-01)

TRANSGENIC APPROACHES TO COMBAT FUSARIUM HEAD BLIGHT IN WHEAT AND BARLEY. - (Peer Reviewed Journal)

Dahleen, L.S., Okubara, P.A., Blechl, A.E. 2001. Transgenic approaches to combat fusarium head blight in wheat and barley. Crop Science 40:628-637.

TRANSGENIC APPROACHES TO COMBAT FUSARIUM HEAD BLIGHT IN WHEAT AND BARLEY. - (Abstract Only)

Dahleen, L.S., Okubara, P.A., Blechl, A.E. 1999. Transgenic approaches to combat fusarium head blight in wheat and barley. Agronomy Abstracts, p. 83.