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United States Department of Agriculture

Agricultural Research Service

Related Topics

Charlene Tanaka
Crop Improvement & Utilization Research
Plant Physiologist

Phone: (510) 559-5776
Fax: (510) 559-5818
Room 2117
USDA, ARS, PWA, WRRC-CIU
800 BUCHANAN STREET
ALBANY, CA, 94710


Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Protein composition of wheat gluten polymer fractions determined by quantitative two-dimensional gel electrophoresis and tandem mass spectrometry Reprint Icon - (Peer Reviewed Journal)
Vensel, W.H., Tanaka, C.K., Altenbach, S.B. 2014. Protein composition of wheat gluten polymer fractions determined by quantitative two-dimensional gel electrophoresis and tandem mass spectrometry. Proteome Science. DOI:10.1186/1477-5956-12-8.
Quantitative proteomic analysis of wheat grain proteins reveals differential effects of silencing of omega-5 gliadin genes in transgenic lines - (Peer Reviewed Journal)
Altenbach, S.B., Tanaka, C.K., Allen, P.V. 2014. Quantitative proteomic analysis of wheat grain proteins reveals differential effects of silencing of omega-5 gliadin genes in transgenic lines. Journal of Cereal Science. 59:118-125.
Modification of vital wheat gluten with phosphoric acid to produce high free solution capacity - (Peer Reviewed Journal)
Robertson, G.H., Cao, T., Gregorski, K.S., Hurkman II, W.J., Tanaka, C.K., Chiou, B., Glenn, G.M., Orts, W.J. 2013. Modification of vital wheat gluten with phosphoric acid to produce high free solution capacity. Journal of Applied Polymer Science. DOI: 10.1002/app.39440.
Comparative proteomic analysis of the effect of temperature and fertilizer on gliadin and glutenin accumulation in the developing endosperm and flour from Triticum aestivum L. cv. Butte 86 - (Peer Reviewed Journal)
Hurkman II, W.J., Tanaka, C.K., Vensel, W.H., Thilmony, R.L., Altenbach, S.B. 2013. Comparative proteomic analysis of the effect of temperature and fertilizer on gliadin and glutenin accumulation in the developing endosperm and flour from Triticum aestivum L. cv. Butte 86. Proteome Science. doi:10.1186/1477-5956-11-8.
Differences in alcohol-soluble protein from genetically altered wheat using capillary zone electrophoresis, one- and two-dimensional electrophoresis and a novel gluten matrix association factor analysis - (Peer Reviewed Journal)
Robertson, G.H., Hurkman II, W.J., Anderson, O.D., Tanaka, C.K., Cao, T., Orts, W.J. 2012. Differences in alcohol-soluble protein from genetically altered wheat using capillary zone electrophoresis, one- and two-dimensional electrophoresis and a novel gluten matrix association factor analysis. Cereal Chemistry. 90(1):13-23. DOI: 10.1094/cchem-10-11-0123.
Physical characteristics of genetically-altered wheat related to technological protein separation - (Peer Reviewed Journal)
Robertson, G.H., Blechl, A.E., Hurkman II, W.J., Anderson, O.D., Cao, T., Tanaka, C.K., Gregorski, K.S., Orts, W.J. 2013. Physical characteristics of genetically-altered wheat related to technological protein separation. Cereal Chemistry. 90(1):1-12.
Wheat flour exposed to ethanol yields dough with unexpected properties Reprint Icon - (Peer Reviewed Journal)
Robertson, G.H., Hurkman Ii, W.J., Cao, T., Gregorski, K.S., Tanaka, C.K., Glenn, G.M., Orts, W.J. 2011. Wheat flour exposed to ethanol yields dough with unexpected properties. Journal of Cereal Science. DOI:10.1094/CCHEM-03-11-0041.
Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE - (Peer Reviewed Journal)
Altenbach, S.B., Tanaka, C.K., Hurkman Ii, W.J., Whitehand, L.C., Vensel, W.H., Dupont, F.M. 2011. Differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-DE. Proteome Science. 9:46.
Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry - (Peer Reviewed Journal)
Dupont, F.M., Vensel, W.H., Tanaka, C.K., Hurkman II, W.J., Altenbach, S.B. 2011. Deciphering the complexities of the wheat flour proteome using quantitative two-dimensional electrophoresis, three proteases and tandem mass spectrometry. Proteome Science. 9(10) available:http://www.proteomesci.com/content/9/1/10.
Finding the Bio in Biobased Products: Electrophoretic Identification of Wheat Proteins in Processed Products - (Peer Reviewed Journal)
Robertson, G.H., Hurkman Ii, W.J., Cao, T., Tanaka, C.K., Orts, W.J. 2010. Finding the Bio in Biobased Products: Electrophoretic Identification of Wheat Proteins in Processed Products. Journal of Agricultural Food & Chemistry. 2010 April 14;58(7):4169-79.
The effect of high temperature on cell structure and gluten protein accumulation in the endosperm of the developing wheat (Triticum aestivum L.) grain - (Proceedings)
Hurkman Ii, W.J., Tanaka, C.K., Thilmony, R.L., Williams, T.G., Wood, D.F., Vensel, W.H., Dupont, F.M., Altenbach, S.B. 2010. The effect of high temperature on cell structure and gluten protein accumulation in the endosperm of the developing wheat (Triticum aestivum L.) grain. International Gluten Workshop. pp. 50-54.
Differential effects of mineral nutrition on wheat flour proteins determined by quantitative 2-DE and MS/MS - (Proceedings)
Dupont, F.M., Altenbach, S.B., Vensel, W.H., Tanaka, C.K., Hurkman II, W.J. 2010. Differential effects of mineral nutrition on wheat flour proteins determined by quantitative 2-DE and MS/MS. International Gluten Workshop. p.64-67.
Effect of high temperature on cell structure and gluten protein accumulation in the endosperm of the developing wheat (Triticum aestivum L.) grain - (Abstract Only)
Hurkman Ii, W.J., Tanaka, C.K., Wood, D.F., Vensel, W.H., Altenbach, S.B., Dupont, F.M. 2010. Effect of high temperature on cell structure and gluten protein accumulation in the endosperm of the developing wheat (Triticum aestivum L.) grain. International Gluten Workshop. p. 50.
Differential effects of mineral nutrition on wheat flour proteins determined by quantitative 2-DE and MS/MS - (Abstract Only)
Dupont, F.M., Altenbach, S.B., Vensel, W.H., Tanaka, C.K., Hurkman Ii, W.J. 2010. Differential effects of mineral nutrition on wheat flour proteins determined by quantitative 2-DE and MS/MS. Xth international Gluten Workshop, Clermont-Ferrand, France, September 7-9, 2009.
Applying Genetic Engineering to Understand the Contributions of Various Seed Proteins to Wheat Flour Properties - (Abstract Only)
Blechl, A.E., Lin, J.W., Altenbach, S.B., Hurkman Ii, W.J., Sloane, S.M., Tanaka, C.K., Vensel, W.H. 2010. Applying Genetic Engineering to Understand the Contributions of Various Seed Proteins to Wheat Flour Properties. Xth International Gluten Workshop, September 7-9, 2009, Clermont-Ferrand, France.
Mass Spectrometric Identification of the Arginine and Lysine deficient Proline Rich Glutamine Rich Wheat Storage Proteins - (Abstract Only) - (12-Jan-09)
The surface-associated proteins of wheat starch granules: suitability of wheat starch for celiac patients - (Peer Reviewed Journal)
Kasarda, D.D., Dupont, F.M., Vensel, W.H., Altenbach, S.B., Lopez, R., Tanaka, C.K., Hurkman II, W.J. 2008. The surface-associated proteins of wheat starch granules: suitability of wheat starch for celiac patients. Journal of Agricultural and Food Chemistry. 56:10292-10302.
Effect of High Temperature on Albumin and Globulin Accumulation in the Endosperm Proteome of the Developing Wheat Grain - (Peer Reviewed Journal)
Hurkman II, W.J., Vensel, W.H., Tanaka, C.K., Whitehand, L.C., Altenbach, S.B. 2009. Effect of High Temperature on Albumin and Globulin Accumulation in the Endosperm Proteome of the Developing Wheat Grain. Journal of Cereal Science. 49:12-23.
Expression of globulin-2, a member of the cupin superfamily of proteins with similarity to known food allergens, is increased under high temperature regimen during wheat grain development - (Peer Reviewed Journal)
Altenbach, S.B., Tanaka, C.K., Hurkman II, W.J., Vensel, W.H. 2009. Expression of globulin-2, a member of the cupin superfamily of proteins with similarity to known food allergens, is increased under high temperature regimen during wheat grain development. Journal of Cereal Science. 49:47-54.
Effect of High Temperature on Albumin and Globulin Accumulation in the Endosperm Proteome of the Developing Wheat Grain - (Abstract Only)
Vensel, W.H., Hurkman II, W.J., Tanaka, C.K., Whitehand, L.C., Altenbach, S.B. 2008. Effect of High Temperature on Albumin and Globulin Accumulation in the Endosperm Proteome of the Developing Wheat Grain. Association of Biomolecular Resource Facilities, 2008 Annual Meeting, February 9-12, 2008, Salt Lake City, Utah.
Effects of Mineral Nutrition and Temperature on Accumulation of Gluten Proteins are Related to Their Content of CYs and Met - (Book / Chapter)
Dupont, F.M., Hurkman, W.J., Vensel, W.H., Chan, R., Tanaka, C.K., Altenbach, S.B. 2007. Effects of Mineral Nutrition and Temperature on Accumulation of Gluten Proteins are Related to Their Content of CYs and Met. In: George L. Lookhart, Perry K. W. Ng, editors. Gluten Proteins 2006. Minneapolis, MN: AACCI. p. 38-42.
Thioredoxin-linked proteins are reduced during germination of medicago truncatula seeds - (Peer Reviewed Journal)
Alkhalfiour, F., Renard, M., Wong, J., Tanaka, C.K., Vensel, W.H., Hurkman, W.J., Buchanan, B.B., Montichard, F. 2007. Thioredoxin-linked proteins are reduced during germination of medicago truncatula seeds. Plant Physiology. 144: 1559-1579.
Genes Encoding the PR-4 Protein Wheatwin Are Developmentally Regulated in Wheat Grains and Respond to High Temperatures During Grainfill - (Peer Reviewed Journal)
Altenbach, S.B., Kothari, K.M., Tanaka, C.K., Hurkman, W.J. 2007. Genes encoding the PR-4 protein wheatwin are developmentally regulated in wheat grains and respond to high temperatures during grainfill. Plant Science. 173(2):135-143.
Expression of 9 kDa lipid transfer protein genes in developing wheat grains is enhanced by high temperature but not by post-anthesis fertilizer - (Peer Reviewed Journal)
Altenbach, S.B., Kothari, K.M., Tanaka, C.K., Hurkman, W.J. 2008. Expression of 9 kDa lipid transfer protein genes in developing wheat grains is enhanced by high temperature but not by post-anthesis fertilizer. Journal of Cereal Science. 47 (2):201-213.
Extraction of Wheat Endosperm Proteins for Proteome Analysis - (Peer Reviewed Journal)
Hurkman, W.J., Tanaka, C.K. 2007. Extraction of Wheat Endosperm Proteins for Proteome Analysis. Journal of Chromatography. 849: 344-350.
High-Resolution Two-Dimensional Gel Electrophoresis: A Cornerstone of Plant Proteomics - (Book / Chapter)
Hurkman, W.J., Tanaka, C.K. 2007. High-Resolution Two-Dimensional Gel Electrophoresis: A Cornerstone of Plant Proteomics. In: Samaj, J and Thelen, J (eds.). Plant Proteomics. Berlin, Germany: Springer-Verlag. p. 14-28.
Differential Accumulation of Sulfur-rich and Sulfur-poor Wheat Flour Proteins is Affected by Temperature and Mineral Nutrition during Grain Development - (Peer Reviewed Journal)
Dupont, F.M., Hurkman, W.J., Vensel, W.H., Chan, R., Lopez, R., Tanaka, C.K., Altenbach, S.B. 2006. Accumulation of sulfur-rich and sulfur-poor wheat flour proteins are affected by temperature and mineral nutrition during grain development. Journal of Cereal Science. 44(1): 101-112
Protein Accumulation and Composition in Wheat Grains: Effects of Mineral Nutrients and High Temperature - (Peer Reviewed Journal)
Dupont, F.M., Hurkman, W.J., Vensel, W.H., Tanaka, C., Kothari, K.M., Chung, O.K., Altenbach, S.B. 2006. Protein accumulation and composition in wheat grains: Effects of mineral nutrients and high temperature. 25(2):96-107.
Proteome Mapping of Mature Pollen of Arabidopsis Thaliana Reprint Icon - (Peer Reviewed Journal)
Holmes-Davis, R.A., Tanaka, C.K., Vensel, W.H., Hurkman, W.J., McCormick, S. 2005. Proteome mapping of mature pollen of Arabidopsis thaliana. Proteomics. 5(18):4864-84.
Developmental Changes in the Metabolic Protein Profiles of Wheat Endosperm - (Peer Reviewed Journal)
Vensel, W.H., Tanaka, C.K., Cal, N., Wong, J.H., Buchanan, B.B., Hurkman, W.J. 2005. Developmental changes in the metabolic protein profiles of wheat endosperm. Encyclopedia of Diagnostic Genomics and Proteomics.78:53-61
High Resolution Two-Dimensional Polyacrylamide Gel Electrophoresis of Wheat Endosperm Proteins for Proteome Analysis - (Peer Reviewed Journal)
Hurkman, W.J., Tanaka, C.K. 2004. High resolution two-dimensional polyacrylamide gel electrophoresis of wheat endosperm proteins for proteome analysis. Journal of Cereal Science.40:295-299.
Construction and Evaluation of Cdna Libraries for Large Scale Est Sequencing in Wheat (Triticum Aestivum L.) - (Peer Reviewed Journal)
Zhang, D., Choi, D.W., Wanamaker, S., Fenton, R.D., Chin, A., Malatrasi, M., Turuspekov, Y., Walia, H., Akhunov, E., Kianian, P., Otto, C., Simons, K., Deal, K., Echenique, V., Stamova, B., Ross, K., Butler, E., Doherty, L., Verhey, S.D., Johnson, R., Altenbach, S.B., Kothari, K.M., Tanaka, C.K., Shah, M.M., Chingcuanco, D.L., Gitt, M.A., Pham, J., Han, P., Miller, R.E., Crossman, C.C., Chao, S., Lazo, G.R., Klueva, N., Gustafson, J.P., Kianian, S.F., Dubcovsky, J., Walker-Simmons, M.K., Gill, K.S., Dvorak, J., Anderson, O.D., Mcguire, P., Qualset, C.O., Nguyen, H.T., Close, T.J. 2004. Construction and evaluation of cdna libraries for large scale est sequencing in wheat (triticum aestivum l.). Genetics. 168:595-608.
Thioredoxin Targets of Developing Wheat Seeds Identified by Complementary Proteomics Approaches. - (Other)
Wong, J.H., Cai, N., Balmer, Y., Tanaka, C.K., Vensel, W.H., Hurkman, W.J., Buchanan, B.B. 2004. Thioredoxin targets of developing wheat seeds identified by complementary proteomics approaches.. Phytochemistry. 65:1629-1640
Tioredoxin Reduction Alters the Solubility of Proteins of Wheat Starchy Endosperm: An Early Event in Cereal Germination. - (Peer Reviewed Journal)
Wong, J.H., Cai, N., Tanaka, C.K., Vensel, W.H., Hurkman, W.J., Buchanan, B.B. 2004. Tioredoxin reduction alters the solubility of proteins of wheat starchy endosperm: an early event in cereal germination.. Plant And Cell Physiology.2004. 45: 407-415
Thioredoxin Links Redox to the Regulation of Fundamental Processes of Plant Mitochondria - (Peer Reviewed Journal)
Balmer, Y., Vensel, W.H., Tanaka, C.K., Hurkman, W.J., Gelhaye, E., Rouhier, N., Hacquot, J., Manieri, W., Schurmann, P., Buchanan, B.B. 2004. Thioredoxin links redox to the regulation of fundamental processes of plant mitochondria. Proceedings of the National Academy of Sciences. 2004. 101: 2642-2647
Analysis of Wheat Endosperm Proteins During Grain Development and in Response to High Temperature Using Proteomics - (Abstract Only)
Hurkman, W.J., Tanaka, C.K., Vensel, W.H., Wong, J.H., Balmer, Y., Buchanan, B.B. 2004. Analysis of wheat endosperm proteins during grain development and in response to high temperature using proteomics. International Gluten Workshop. p. 169-172
Unraveling Thioredoxin-Linked Metabolic Processes of Cereal Starchy Endosperm Using Proteomics - (Peer Reviewed Journal)
Wong, J.H., Balmer, Y., Cai, N., Tanaka, C.K., Vensel, W.H., Hurkman, W.J., Buchanan, B.B. 2003. Unraveling thioredoxin-linked metabolic processes of cereal starchy endosperm using proteomics. Federation of European Biochemical Societies Letters.2003. 547: 151-156
Effect of Temperature on Expression of Genes Encoding Enzymes for Starch Biosynthesis in Developing Wheat Endosperm - (Peer Reviewed Journal)
Hurkman, W.J., Mc Cue, K.F., Altenbach, S.B., Korn, A.M., Tanaka, C.K., Kothari, K.M., Bechtel, D.B., Wilson, J.D., Anderson, O.D., Dupont, F.M. 2003. Effect of temperature on expression of genes encoding enzymes for starch biosynthesis in developing wheat endosperm. Plant Science. 164(5):873-881.
Effect of Temperature on Expression of Genes Encoding Enzymes for Starch Biosynthesis in Developing Wheat Endosperm - (Peer Reviewed Journal)
HURKMAN, W.J., MCCUE,K.F., ALTENBACH, S.B., KORN, A.M., TANAKA, C.K., KOTHARI, K.M., BECHTEL, D.B., WILSON, J.D., ANDERSON, O.D., DUPONT, F.M. EFFECT OF TEMPERATURE ON EXPRESSION OF GENES ENCODING ENZYMES FOR STARCH BIOSYNTHESIS IN DEVELOPING WHEAT ENDOSPERM. PLANT SCIENCE. 2003. 164: 873-881
Last Modified: 7/30/2014