Projects

STRATEGIES TO CONTROL AND PREVENT BACTERIAL INFECTIONS IN SWINE Appropriated (D)   Accession Number: 421211

COMPARISON OF PORCINE HIGH FEVER DISEASE ISOLATES OF PRRSV TO US ISOLATES FOR THEIR ABILITY TO CAUSE SECONDARY BACTERIAL INFECTION IN SWINE Reimbursable (R)   Accession Number: 422165

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Transcriptome profiling reveals stage-specific production and requirement of flagella during biofilm development in Bordetella bronchiseptica - (Peer Reviewed Journal)

Nicholson, T.L., Conover, M.S., Deora, R. 2012. Transcriptome profiling reveals stage-specific production and requirement of flagella during biofilm development in Bordetella bronchiseptica. PLoS ONE. 7(11):e49166.

Changes in circulating and thymic lymphocyte populations following infection with strains of North American or highly pathogenic PRRSV - (Abstract Only)

Loving, C.L., Brockmeier, S.L., Palmer, M.V., Spear, A.R., Faaberg, K.S., Nicholson, T.L. 2012. Changes in circulating and thymic lymphocyte populations following infection with strains of North American or highly pathogenic PRRSV. International PRRS Symposium 2012. Poster No. 77.

Comparison of the pathogenesis of Asian highly-pathogenic PRRSV isolates to U.S. isolates and their ability to cause secondary bacterial infection in swine - (Abstract Only)

Brockmeier, S.L., Loving, C.L., Palmer, M.V., Spear, A.R., Faaberg, K.S., Nicholson, T.L. 2012. Comparison of the pathogenesis of Asian highly-pathogenic PRRSV isolates to U.S. isolates and their ability to cause secondary bacterial infection in swine. International PRRS Symposium 2012. Poster No. 64.

Changes in circulating and thymic lymphocyte populations following infection with strains of North American or highly pathogenic PRRSV - (Abstract Only)

Loving, C.L., Brockmeier, S.L., Palmer, M.V., Spear, A.R., Faaberg, K.S., Nicholson, T.L. 2012. Changes in circulating and thymic lymphocyte populations following infection with strains of North American or highly pathogenic PRRSV. Conference of Research Workers in Animal Diseases Annual Meeting. Paper No. 185.

Comparison of Asian highly-pathogenic PRRSV isolates to US isolates for their ability to cause secondary bacterial infection in swine - (Abstract Only)

Brockmeier, S.L., Loving, C.L., Palmer, M.V., Spear, A.R., Faaberg, K.S., Nicholson, T.L. 2012. Comparison of Asian highly-pathogenic PRRSV isolates to US isolates for their ability to cause secondary bacterial infection in swine. Conference of Research Workers in Animal Diseases. Paper No. 184.

Infection of United States swine with a Chinese highly pathogenic strain of porcine reproductive and respiratory syndrome virus - (Peer Reviewed Journal)

Guo, B., Lager, K.M., Henningson, J.N., Miller, L.C., Schlink, S.N., Kappes, M.A., Kehrli, Jr., M.E., Brockmeier, S.L., Nicholson, T.L., Yang, H., Faaberg, K.S. 2013. Experimental infection of United States swine with a Chinese highly pathogenic strain of porcine reproductive and respiratory syndrome virus. Virology. 435(2):372-384.

Genomic sequence and virulence comparison of four type 2 porcine reproductive and respiratory syndrome virus strains - (Peer Reviewed Journal)

Brockmeier, S.L., Loving, C.L., Vorwald, A.C., Kehrli, Jr., M.E., Baker, R.B., Nicholson, T.L., Lager, K.M., Miller, L.C., Faaberg, K.S. 2012. Genomic sequence and virulence comparison of four Type 2 porcine reproductive and respiratory syndrome virus strains. Virus Research. 169(1):212-221.

The presence of alpha interferon at the time of infection alters the innate and adaptive immune responses to porcine reproductive and respiratory syndrome virus - (Peer Reviewed Journal)

Brockmeier, S.L., Loving, C.L., Nelson, E.A., Miller, L.C., Nicholson, T.L., Register, K.B., Grubman, M.J., Brough, D.E., Kehrli, Jr., M.E. 2012. The presence of alpha interferon at the time of infection alters the innate and adaptive immune responses to porcine reproductive and respiratory syndrome virus. Clinical and Vaccine Immunology. 19(4):508-514.

Effects of interferon-alpha on the immune response to porcine reproductive and respiratory syndrome virus - (Peer Reviewed Journal) - (01-Jan-12)

Phenotypic modulation of the Bvg+ phase is not required for pathogenesis and transmission of Bordetella bronchiseptica in swine - (Peer Reviewed Journal)

Nicholson, T.L., Brockmeier, S.L., Loving, C.L., Register, K.B., Kehrli, Jr., M.E., Stibitz, S.E., Shore, S.M. 2012. Phenotypic modulation of the virulent Bvg phase is not required for pathogenesis and transmission of Bordetella bronchiseptica in swine. Infection and Immunity. 80(3):1025-1036.

Swine MRSA isolates form robust biofilms - (Abstract Only)

Nicholson, T.L. 2011. Swine MRSA isolates form robust biofilms [abstract]. Conference of Research Workers in Animal Diseases. Paper No. 99.

Sequence and virulence comparison of four North American isolates of porcine reproductive and respiratory syndrome virus - (Abstract Only)

Brockmeier, S.L., Loving, C.L., Miller, L.C., Vorwald, A.C., Kehrli, Jr., M.E., Baker, R.B., Nicholson, T.L., Lager, K.M., Faaberg, K.S. 2011. Sequence and virulence comparison of four North American isolates of porcine reproductive and respiratory syndrome virus. Conference of Research Workers in Diseases. Paper No. 174. p. 171.

Fulminant sepsis is a cardinal sign of HP-PRRSV in pigs - (Abstract Only)

Lager, K.M., Faaberg, K.S., Guo, B., Brockmeier, S.L., Henningson, J.N., Schlink, S.N., Miller, L.C., Kappes, M.A., Kehrli, Jr., M.E., Nicholson, T.L., Yang, H.-C. 2011. Fulminant sepsis is a cardinal sign of HP-PRRSV in pigs [abstract]. 2011 International PRRS Symposium. Paper No. 60. page 77.

Comparison of the pathogenicity of Chinese and low virulent US porcine reproductive and respiratory syndrome viruses - (Abstract Only)

Henningson, J.N., Faaberg, K.S., Guo, B., Schlink, S.N., Miller, L.C., Kappes, M.A., Kehrli, Jr., M.E., Brockmeier, S.L., Nicholson, T.L., Vorwald, A.C., Lager, K.M. 2011. Comparison of the pathogenicity of Chinese and low virulent US porcine reproductive and respiratory syndrome viruses [abstract]. American College of Veterinary Pathologists. Paper No. 164. Veterinary Pathology. 48(6):E30.

Bordetellosis - (Book / Chapter)

Brockmeier, S.L., Register, K.B., Nicholson, T.L., Loving, C.L. 2012. Bordetellosis. In: Zimmerman, J., Karriker, L., Ramirez, A., Schwartz, K., Stevenson, G., editors.Diseases of Swine. 10th edition. Ames, IA: Iowa State University Press. pp. 670-679.

HP-PRRSV challenge of 4 and 10-week-old pigs - (Proceedings)

Lager, K.M., Faaberg, K.S., Guo, B., Brockmeier, S., Henningson, J.N., Schlink, S.N., Miller, L.C., Kappes, M.A., Kehrli, Jr., M.E., Loving, C.L., Nicholson, T.L., Yang, H.C. 2011. HP-PRRSV challenge of 4 and 10-week-old pigs. In: Proceedings of the 5th Asian PRRSpective Symposium, May 10, 2011, Bangkok, Thailand. 6 pages.

Porcine TLR3 characterization and expression in response to influenza virus and Bordetella bronchiseptica - (Peer Reviewed Journal)

Sacco, R.E., Nicholson, T.L., Waters, T.E., Brockmeier, S. 2011. Porcine TLR3 characterization and expression in response to influenza virus and Bordetella bronchiseptica. Veterinary Immunology and Immunopathology. 142(1-2):57-63.

Contribution of Bordetella bronchiseptica Type III secretion system to respiratory disease in swine - (Abstract Only)

Brockmeier, S., Nicholson, T.L., Loving, C.L., Register, K.B. 2011. Contribution of Bordetella bronchiseptica type III secretion system to respiratory disease in swine [abstract]. American Society for Microbiology 2011 Annual Meeting. Abstract No. 1494.

Utility of a Panviral Microarray for Detection of Swine Respiratory Viruses in Clinical Samples - (Peer Reviewed Journal)

Nicholson, T.L., Kukielka, D., Vincent, A.L., Brockmeier, S.L., Miller, L.C., Faaberg, K.S. 2011. Utility of a panviral microarray for detection of swine respiratory viruses in clinical samples. Journal of Clinical Microbiology. 49(4):1542-1548.

Use of an Avirulent Isolate of Haemophilus parasuis as a Vaccine - (Abstract Only)

Brockmeier, S., Register, K.B., Loving, C.L., Nicholson, T.L. 2010. Use of an avirulent Isolate of Haemophilus parasuis as a vaccine. 45th US-Japan Cooperative Program in Natural Resources Panel of Animal and Avian Health Meeting. p 14.

Evaluation of the Specificity of BP3385 for Bordetella pertussis - (Peer Reviewed Journal)

Register, K.B., Nicholson, T.L., Guthrie, J.L. 2010. Evaluation of the Specificity of BP3385 for Bordetella pertussis. Journal of Clinical Microbiology. 48(9):3334-3337.

Influenza Virus Coinfection with Bordetella bronchiseptica Enhances Bacterial Colonization and Host Responses Exacerbating Pulmonary Lesions - (Peer Reviewed Journal)

Loving, C.L., Brockmeier, S.L., Vincent, A.L., Palmer, M.V., Sacco, R.E., Nicholson, T.L. 2010. Influenza Virus Coinfection with Bordetella bronchiseptica Enhances Bacterial Colonization and Host Responses Exacerbating Pulmonary Lesions. Microbial Pathogenesis. 49(5):237-245.

Prior Colonization with an Avirulent Haemophilus parasuis Isolate Prevents Disease from a Subsequent Virulent Challenge with a Heterologous Isolate - (Proceedings)

Brockmeier, S., Loving, C.L., Mullins, M., Register, K.B., Nicholson, T.L. Prior Colonization with an Avirulent Haemophilus parasuis Isolate Prevents Disease from a Subsequent Virulent Challenge with a Heterologous Isolate. In: Proceedings of the Pig Veterinary Society International Congress, July 18-21, Vancouver, Canada. p. 192.

Contribution of the Bordetella Bps Polysaccharide to Biofilm Formation and Respiratory Disease in Swine - (Abstract Only)

Nicholson, T.L., Brockmeier, S., Loving, C.L., Lu, H., Sloan, G.P., Deora, R. 2010. Contribution of the Bordetella Bps Polysaccharide to Biofilm Formation and Respiratory Disease in Swine [abstract]. American Society for Microbiology. p. 123.

Utility of a Panviral Microarray for Detection of Swine Respiratory Viruses in Clinical Samples - (Abstract Only)

Nicholson, T.L., Kukielka, D., Wang, D., Faaberg, K.S. 2009. Detection Limits of PRRSV and SIV Using Pan Viral Microarray [abstract]. 45th Meeting of the United States-Japan Cooperative Program in Natural Resources Panel of Animal and Avian Health, December 2-4, 2009, Tokyo, Japan. p. 21.

Swine influenza virus co-infection with Bordetella bronchiseptica enhances bacterial colonization and host immune responses exacerbating pulmonary lesions - (Abstract Only)

Loving, C.L., Brockmeier, S.L., Vincent, A.L., Palmer, M.V., Sacco, R.E., Nicholson, T.L. 2010. Swine influenza virus co-infection with Bordetella bronchiseptica enhances bacterial colonization and host immune responses exacerbating pulmonary lesions [abstract]. Conference Research Workers Disease Meeting. Paper No. 130.

Virulence and Genome Evolution of Bordetella bronchiseptica - (Abstract Only) - (07-Oct-09)

Studies of pathogenesis, transmission, heterologous protection, and genomics of four isolates of Haemophilus parasuis - (Abstract Only)

Brockmeier, S., Loving, C.L., Register, K.B., Nicholson, T.L., Bayles, D.O., Phillips, G.J. 2009. Studies of pathogenesis, transmission, heterologous protection, and genomics of four isolates of Haemophilus parasuis [abstract]. 2009 Conference of Research Workers in Diseases. Abstract No. 15, p. 118.

Studies of Pathogenesis, Transmission, and Heterologous Protection of Four Isolates of Haemophilus parasuis in a Highly Susceptible Swine Population - (Abstract Only)

Brockmeier, S., Loving, C.L., Register, K.B., Nicholson, T.L., Bayles, D.O., Phillips, G.J. 2010. Studies of pathogenesis, transmission, and heterologous protection of four isolates of Haemophilus parasuis in a highly susceptible swine population [abstract]. 2010 American Association of Swine Veterinarians Annual Meeting. p. 127-129.

Microarray and functional analysis of growth-phase dependent gene regulation in Bordetella bronchiseptica - (Peer Reviewed Journal)

Nicholson, T.L., Buboltz, A.M., Harvill, E.T., Brockmeier, S.L. 2009. Microarray and Functional Analysis of Growth-Phase Dependent Gene Regulation in Bordetella bronchiseptica. Infection and Immunity. 77(10):4221-4231.

Characterization and comparative analysis of the genes encoding Haemophilus parasuis outer membrane proteins P2 and P5 - (Peer Reviewed Journal)

Mullins, M.A., Register, K.B., Bayles, D.O., Loving, C.L., Nicholson, T.L., Brockmeier, S.L., Dyer, D.W., Phillips, G.J. 2009. Characterization and Comparative Analysis of the Genes Encoding Haemophilus parasuis Outer Membrane Proteins P2 and P5. Journal of Bacteriology. 191(19):5988-6002.

Enhanced Type III Secretion System activity contributes to the increased virulence of a Bordetella bronchiseptica lineage - (Peer Reviewed Journal)

Buboltz, A.M., Nicholson, T.L., Weyrich, L.S., Harvill, E.T. 2009. Role of the Type III Secretion System in a Hypervirulent Lineage of Bordetella bronchiseptica. Infection and Immunity. 77(9):3969-3977.

Evidence for horizontal gene transfer of two antigenically distinct O antigens in Bordetella bronchiseptica - (Peer Reviewed Journal)

Buboltz, A., Nicholson, T.L., Karanikas, A., Preston, A., Harvill, E. 2009. Evidence for horizontal gene transfer of two antigenically distinct O antigens in Bordetella bronchiseptica. Infection and Immunity. 77(8):3249-3257.

Comparative Genomic Hybridization Suggests a Unique Genetic Basis for Virulence of Bordetella hinzii - (Abstract Only)

Register, K.B., Nicholson, T.L. 2009. Comparative genomic hybridization suggests a unique genetic basis for virulence of Bordetella hinzii [abstract]. 109th American Society for Microbiology General Meeting. Poster No. Z-038.

Identification and Comparative Analysis of Genes Encoding Outer Membrane Proteins P2 and P5 in Haemophilus parsuis - (Abstract Only)

Mullins, M.A., Register, K.B., Bayles, D.O., Loving, C.L., Nicholson, T.L., Brockmeier, S.L., Dyer, D.W., Phillips, G.J. 2009. Identification and comparative analysis of genes encoding outer membrane proteins P2 and P5 in Haemophilus parsuis [abstract]. 109th American Society for Microbiology General Meeting. Poster No. Z-034.

Contribution of Bordetella bronchiseptica Filamentous Hemagglutinin and Pertactin to Respiratory Disease in Swine - (Peer Reviewed Journal)

Nicholson, T.L., Brockmeier, S., Loving, C.L. 2009. Contribution of Bordetella bronchiseptica Filamentous Hemagglutinin and Pertactin to Respiratory Disease in Swine. Infection and Immunity. 77(5):2136-2146.

Replacement of adenylate cyclase toxin in a lineage of Bordetella bronchiseptica - (Peer Reviewed Journal)

Buboltz, A.M., Nicholson, T.L., Parette, M.R., Hester, S.E., Parkhill, J., Harvill, E. 2008. Replacement of adenylate cyclase toxin in a lineage of Bordetella bronchiseptica. Journal of Bacteriology. 190(15):5502-5511.

Investigating the porcine immune reponse to Haemophilus parasuis - (Abstract Only) - (15-May-08)

Early Induction of Cytokines in Pigs Coinfected with Swine Influenza Virus and Bordetella bronchiseptica - (Proceedings)

Brockmeier, S., Vincent, A.L., Loving, C.L., Nicholson, T.L., Sacco, R.E. 2008. Early Induction of Cytokines in Pigs Coinfected with Swine Influenza Virus and Bordetella bronchiseptica. In: Proceedings of the 20th International Pig Veterinary Society Congress, June 22-26, 2008, Durban, South Africa. 1:10.

Contribution of Bordetella bronchiseptica Filamentous Hemagglutinin and Pertactin to Respiratory Disease in Swine - (Abstract Only)

Nicholson, T.L., Brockmeier, S., Loving, C.L. 2008. Contribution of Bordetella bronchiseptica Filamentous Hemagglutinin and Pertactin to Respiratory Disease in Swine [abstract]. American Society for Microbiology Meeting, June 2-4, 2008, Boston, Massachusetts. 2008 CDROM.

Bordetella bronchiseptica Bvg Phase-Dependent Contribution to Colonization, Transmission, and Immune Responses in Pigs - (Abstract Only)

Loving, C.L., Nicholson, T.L., Brockmeier, S. 2008. Bordetella bronchiseptica Bvg Phase-Dependent Contribution to Colonization, Transmission, and Immune Responses in Pigs [abstract]. American Society for Microbiology Meeting, June 2-4, 2008, Boston, Massachusetts. 2008 CDROM.

Early Induction of Cytokines in Pigs Coinfected with Swine Influenza Virus and Bordetella bronchiseptica - (Abstract Only)

Brockmeier, S., Loving, C.L., Nicholson, T.L., Vincent, A.L., Sacco, R.E. 2008. Early Induction of Cytokines in Pigs Coinfected with Swine Influenza Virus and Bordetella bronchiseptica [abstract]. American Society for Microbiology Meeting, June 2-4, 2008, Boston, Massachusetts. 2008 CDROM.

Replacement of Adenylate Cyclase Toxin in a Prominent Lineage of Bordetella bronchiseptica - (Proceedings)

Buboltz, A.M., Nicholson, T.L., Parette, M.R., Hester, S.E., Parkhill, J., Harvill, E.T. 2008. Replacement of Adenylate Cyclase Toxin in a Prominent Lineage of Bordetella bronchiseptica. Keystone Symposium Molecular Evolution as a Driving Force in Infectious Disease, April 8-13, 2008, Breckenridge, Colorado. p. 16.

Coinfection of Pigs with Swine Influenza Virus and Bordetella bronchiseptica - (Abstract Only)

Brockmeier, S., Vincent, A.L., Nicholson, T.L., Sacco, R.E., Palmer, M.V. 2007. Coinfection of Pigs with Swine Influenza Virus and Bordetella bronchiseptica [abstract]. In: United States Japan Natural Resources Animal and Avian Health Panel, December 5, 2007, Tsukuba, Japan. p. 25.

Growth Phase dependent gene regulation in Bordetella bronchiseptica - (Abstract Only) - (12-Nov-07)

Coinfection of pigs with Porcine Respiratory Coronavirus and Bordetella bronchisphica - (Peer Reviewed Journal)

Brockmeier, S., Loving, C.L., Nicholson, T.L., Palmer, M.V. 2008. Coinfection of pigs with Porcine Respiratory Coronavirus and Bordetella bronchisphica. Veterinary Microbiology. 128(1-2):36-47.

Misidentification of Bordetella bronchiseptica as Bordetella pertussis using a Newly Described RT-PCR Targeting the Pertactin Gene - (Peer Reviewed Journal)

Register, K.B., Nicholson, T.L. 2007. Misidentification of Bordetella bronchiseptica as Bordetella pertussis using a newly described RT-PCR targeting the pertactin gene. Journal of Medical Microbiology. 56:1608-1610.

Coinfection with Swine Influenza Virus and Bordetella bronchiseptica in Pigs - (Abstract Only)

Brockmeier, S., Vincent, A.L., Sacco, R.E., Nicholson, T.L., Palmer, M.V. 2007. Coinfection with Swine Influenza Virus and Bordetella bronchiseptica in Pigs [abstract]. American Society for Microbiology Meeting, May 21-25,2007, Toronto, Canada. 2007 CD-ROM.

MICROARRAY - BASED APPROACH FOR GENOME-WIDE SURVEY OF NUCLEOTIDE POLYMORPHISMS - (Book / Chapter)

Brunelle, B.W., Nicholson, T.L. 2007. Microarray - based approach for genome-wide survey of nucleotide polymorphisms. In: Bergman, Nicholas H., editor. Methods in Molecular Biology. Totowa, NJ: Humana Press. p. 267-280.

Construction and validation of a first-generation long-oligonulceotide microarray by transcriptional of the Bordetella bronchiseptica Bvg regulon - (Peer Reviewed Journal)

Nicholson, T.L. 2007. Construction and validation of a first-generation long-oligonulceotide microarray by transcriptional of the Bordetella bronchiseptica Bvg regulon. Biomed Central (BMC) Genomics. 8(1):220.

Expression Profiling of Bordetella Bronchiseptica Responses to Temperature Using a Long-Oligonucleotide Microarray - (Abstract Only)

Nicholson, T.L., Brockmeier, S., Register, K.B. 2006. Expression Profiling of Bordetella Bronchiseptica Responses to Temperature Using a Long-Oligonucleotide Microarray [abstract]. United States-Japan Cooperative Program in Natural Resources. p. 18.

Loss of Adenylate Cyclase Tonxin among closely related B. bronchiseptica strains - (Abstract Only)

Buboltz, A., Nicholson, T.L., Harvill, E. 2006. Loss of Adenylate Cyclase Tonxin among closely related B. bronchiseptica strains [abstract]. Eighth International Symposium: Saga of the Genus Bordetella, November 7-10, 2006, Pasteur, Paris. p. 26.

EXPRESSION PROFILING OF BORDETELLA BRONCHISEPTICA RESPONSES TO TEMPERATURE USING A LONG-OLIGONUCLEOTIDE MICROARRAY - (Abstract Only)

Nicholson, T.L., Brockmeier, S., Register, K.B. 2005. Expression profiling of bordetella bronchiseptica responses to temperature using a long-oligonucleotide microarray. American Society for Microbiology. D118, p. 218.

COINFECTION WITH PORCINE RESPIRATORY CORONAVIRUS AND BORDETELLA BRONCHISEPTICA IN PIGS - (Abstract Only)

Brockmeier, S., Loving, C.L., Nicholson, T.L., Palmer, M.V. 2005. Coinfection with porcine respiratory coronavirus and bordetella bronchiseptica in pigs. American Society for Microbiology. Z016, p. 614.

EXPRESSION PROFILING OF BORDETELLA BRONCHISEPTICA RESPONSES TO TEMPERATURE USING A LONG-OLIGONUCLEOTIDE MICROARRAY - (Abstract Only)

Nicholson, T.L., Brockmeier, S., Register, K.B. 2005. Expression profiling of Bordetella bronchiseptica responses to temperature using a long-oligonucleotide microarray [abstract]. International Union of Microbiological Societies Proceedings/Abstracts. p. 18.