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Weste Osbrink
 

Adalberto A Perez De Leon (Beto)
Knipling-Bushland U.S. Livestock Insects Research Laboratory
Laboratory Director

Phone: (830) 792-0304
Fax: (830) 792-0302

2700 FREDERICKSBURG RD
KERRVILLE, TX, 78028-1984

Projects
EVALUATION OF IMMUNE RESPONSE OF WHITE-TAILED DEER FOLLOWING VACCINATION WITH ANTI-CATTLE TICK VACCINE CANDIDATE ANTIGENS
General Cooperative Agreement (A)
  Accession Number: 422954
Development of a male-only strain of the new world screwworm, Cochliomyia Hominivorax (Diptera, Calliphoridae)
General Cooperative Agreement (A)
  Accession Number: 423566
MINING THE GENOME OF RHIPICEPHALUS MICROPLUS TO DEVELOP NOVEL CONTROL TECHNOLOGY AND VACCINES
Appropriated (D)
  Accession Number: 416467
BIOLOGY AND CONTROL OF TICKS OF VETERINARY AND HUMAN IMPORTANCE
Appropriated (D)
  Accession Number: 418521
MOLECULAR BIOLOGY AND PHYSIOLOGY OF BITING FLIES AFFECTING LIVESTOCK
Appropriated (D)
  Accession Number: 418533
SERUM CONCENTRATION OF IVERMECTIN IN PASTURED CATTLE PROVIDED FREE-ACCESS TO AN IVERMECTIN-MEDICATED PROTEIN OR MINERAL BLOCK SUPPLEMENT
Nonfunded Cooperative Agreement (N)
  Accession Number: 416682
SYSTEMS BIOLOGY INVESTIGATIONS OF ARTHROPOD-BORNE DISEASES TO CONTROL VECTORS OF PATHOGENS
Nonfunded Cooperative Agreement (N)
  Accession Number: 420713
USE OF SYNERGIZED ACARICIDE FORMULATIONS FOR THE CONTROL OF RESISTANT POPULATIONS OF RHIPICEPHALUS MICROPLUS IN THE MEXICAN TROPICS
Nonfunded Cooperative Agreement (N)
  Accession Number: 420832
INVESTIGATIONS OF NOVEL TARGET-SPECIFIC LIGANDS FOR ACETYLCHOLINESTERASES OF ARTHROPOD PESTS
Nonfunded Cooperative Agreement (N)
  Accession Number: 420982
FUNCTIONAL CHARACTERIZATION AND TARGET VALIDATION OF TICK (BOOPHILUS MICROPLUS) G PROTEIN-COUPLED RECEPTORS
Nonfunded Cooperative Agreement (N)
  Accession Number: 420994
ASSESSMENT OF FECAL CHEMISTRY CHANGES IN FEVER TICK INFESTED CATTLE USING NEAR INFRARED REFLECTANCE SPECTROSCOPY
Nonfunded Cooperative Agreement (N)
  Accession Number: 421933
Evaluation of Immune Response of Canines Following Vaccination with Anti-Tick Vaccine Candidate Antigens
Nonfunded Cooperative Agreement (N)
  Accession Number: 424198
EXPERIMENTAL BM86-BASED VACCINE FOR THE CATTLE FEVER TICK ERADICATION PROGRAM
Nonfunded Cooperative Agreement (N)
  Accession Number: 423237
EVALUATION OF CANDIDATE ANTI-TICK VACCINE ANTIGENS
Nonfunded Cooperative Agreement (N)
  Accession Number: 422472
DEVELOPMENT OF A MALE-ONLY STRAIN OF THE NEW WORLD SCREWWORM, COCHLIOMYIA HOMINIVORAX (DIPTERA, CALLIPHORIDAE)
Specific Cooperative Agreement (S)
  Accession Number: 419925
PRODUCTION AND EFFICACY TESTING OF BM86-TEXAS ANTI-TICK VACCINE FOR THE CATTLE FEVER TICK ERADICATION PROGRAM
Trust (T)
  Accession Number: 422252

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Effects of permethrin and amitraz on gas exchange and water loss in unfed adult females of Amblyomma americanum (Acari: Ixodidae) - (Peer Reviewed Journal) - (18-Dec-12)
Resistance of the tick Rhipicephalus (Boophilus) microplus to ivermectin in Mexico - (Other)
Rodriguez-Vivas, R.I., Perez-Cogollo, L.C., Rosado-Aguilar, J.A., Ojeda-Chi, M.M., Trinidad-Martinez, I., Torres-Acosta, J.F., Miller, R., Li, A.Y., Perez De Leon, A.A. 2012. Resistance of the tick Rhipicephalus (Boophilus) microplus to ivermectin in Mexico. Workshop Proceedings. p. 52-68.
Screening and identification of novel cytochrome P450s in ticks - (Other) - (19-Sep-12)
Acetylcholinesterases of blood-feeding flies and ticks - (Peer Reviewed Journal) - (19-Sep-12)
Pyrosequencing-based analysis of the microbiome associated with the horn fly,Haematobia irritans - (Peer Reviewed Journal) - (07-Aug-12)
Ticks on Deer and Cattle in the Cattle Fever Tick Permanent Quarantine Zone, 2012 - (Abstract Only) - (25-Jun-12)
Stable Fly Project in Campo Grande, Brazil - (Abstract Only) - (19-Jun-12)
Discovery and functional analysis of small RNAs (miRNA/siRNA) of livestock ectoparasites. - (Abstract Only) - (11-Jun-12)
Stable Fly Project in Campo Grande, Brazil - (Abstract Only) - (01-Jun-12)
Exploring the use of anti-tick vaccine as tool for integrated eradication of the cattle fever tick, Rhipicephalus (Boophilus) annulatus - (Peer Reviewed Journal)
Miller, R., Estrada-Pena, A., Almazan, C., Yeater, K.M., Messenger, M., Ellis, D., Perez De Leon, A.A. 2012. Exploring the use of anti-tick vaccine as tool for integrated eradication of the cattle fever tick, Rhipicephalus (Boophilus) annulatus. Vaccine. 30(38):5682-5687.
Integrated strategy for sustainable cattle fever tick eradication in U.S.A. is required to mitigate the impact of global change - (Peer Reviewed Journal)
Perez De Leon, A.A., Pete, T.D., Auclair, A.N., Messenger, M.T., Guerrero, F., Schuster, G., Miller, R. 2012. Integrated strategy for sustainable cattle fever tick eradication in U.S.A. is required to mitigate the impact of global change. Frontiers in Systems Biology. 3:195.
The US experience of eradicating and preventing re-infestation with cattle fever ticks - (Proceedings)
Li, A.Y., Miller, R., Pound, J.M., Guerrero, F., Temeyer, K.B., Perez De Leon, A.A. 2012. The US experience of eradicating and preventing re-infestation with cattle fever ticks. Workshop Proceedings. 4th International Bayer Cattle Symposium, Lisbon, Portugal, June 3, 2012 (4-19).
North American soft ticks (Ornithodoros spp.): biology and feral swine parasitism as risks for the emergence of African swine fever in the U.S.A. - (Abstract Only) - (12-Apr-12)
Global change and integrated approach for sustainable cattle fever tick eradication in the United States of America - (Abstract Only) - (07-Apr-12)
Tick-borne protozoa - (Book / Chapter) - (02-Apr-12)
Anti-cattle tick vaccines: Many candidate antigens, but will a commercially viable product emerge? - (Peer Reviewed Journal)
Guerrero, F., Miller, R., Perez De Leon, A.A. 2012. Anti-cattle tick vaccines: Many candidate antigens, but will a commercially viable product emerge? International Journal for Parasitology. 42(5):421-427.
Pyrosequencing-based analysis of the microbiome associated with the horn fly, Haematobia irritans - (Other) - (01-Apr-12)
A Novel Carbamate Insecticide with Superior Selectivity for Cattle Tick (Boophilus microplus) and Sand Fly (Phlebotomus papatasi) Control - (Abstract Only) - (19-Mar-12)
Acetylcholinesterases of Rhipicephalus (Boophilus) microplus and Phlebotomus papatasi: Gene identification, expression and biochemical properties of recombinant proteins - (Abstract Only) - (19-Mar-12)
Bovine immunoprotection against Rhipicephalus microplus with recombinant Bm86-Campo Grande antigen - (Peer Reviewed Journal) - (14-Mar-12)
Contributions of USDA-ARS Livestock Entomology Research to Meet Food Security Challenge - (Abstract Only) - (05-Mar-12)
Immunoregulation of bovine macrophages by factors in the salivary glands of Rhipicephalus microplus - (Peer Reviewed Journal)
Brake, D.K., Perez De Leon, A.A. 2012. Immunoregulation of bovine macrophages by factors in the salivary glands of Rhipicephalus microplus. Parasites & Vectors. 14(5):Article 38.
Complexity of acetylcholinesterases in biting flies and ticks - (Abstract Only) - (31-Jan-12)
Effects of pyriproxyfen and buprofezin on immature development and reproduction in the stable fly - (Peer Reviewed Journal)
Liu, S.S., Li, A.Y., Lohmeyer, K.H., Perez De Leon, A.A. 2012. Effects of pyriproxyfen and buprofezin on immature development and reproduction in the stable fly. Medical and Veterinary Entomology. doi: 10.1111/j.1365-2915.2012.01013.x.
Innovative technologies targeting vector populations to mitigate the risk of exposure to leishmaniasis and protect deployed U.S. Military personnel in the Middle East - (Abstract Only) - (10-Nov-11)
White-tailed Deer Visitation Rates at Medicated Bait Sites in Southern Texas - (Abstract Only) - (09-Nov-11)
Acaricide Research and Development, Resistance and Resistance Monitoring - (Book / Chapter) - (28-Oct-11)
Innovative technologies targeting vector populations to mitigate the risk of exposure to leishmaniasis and protect deployed U.S. Military personnel in the Middle East - (Abstract Only) - (11-Oct-11)
Evolving translational research to keep the U.S. free of cattle fever ticks - (Abstract Only) - (15-Sep-11)
Biological control as a tool to mitigate economic impacts of facilitative ecological interactions between the giant reed and cattle fever ticks - (Abstract Only) - (11-Sep-11)
Facilitative ecological interactions between invasive species: Arundo donax (Poaceae) stands as favorable habitat for cattle ticks (Acari: Ixodidae) along the U.S.-Mexico border - (Peer Reviewed Journal)
Racelis, A.E., Davey, R.B., Goolsby, J., Perez De Leon, A.A., Varner, K., Duhaime, R. 2012. Facilitative ecological interactions between invasive species: Arundo donax (Poaceae) stands as favorable habitat for cattle ticks (Acari: Ixodidae) along the U.S.-Mexico border. Journal of Medical Entomology. 49(2):410-417.
Biogenic amines in the stable fly Stomoxys calcitrans L. (Diptera: Muscidae): tissue localization and roles in feeding and reproduction - (Abstract Only) - (23-Jun-11)
Challenges for the Control and Eradication of the Tropical Cattle Tick Rhipicephalus microplus and Perspectives of Anti-tick vaccines - (Abstract Only) - (23-Jun-11)
Integration of ecologically-based approaches to re-eradicate cattle fever ticks from the U.S. - (Abstract Only) - (11-Jun-11)
Immunoprotection of cattle vaccinated with rRmLTI against Rhipicephalus microplus - (Abstract Only) - (28-May-11)
Evaluation of the VetCap® treatment method for horn fly control in cattle - (Peer Reviewed Journal)
Li, A.Y., Ross, D., Perez De Leon, A.A. 2011. Evaluation of the VetCap® treatment method for horn fly control in cattle. International Journal of Applied Research in Veterinary Medicine. 9(2):198-203.
Preliminary findings of a molecular survey for the presence of B. bovis and B. bigemina in cattle fever ticks and white-tailed deer from south Texas - (Proceedings) - (16-May-11)
Assessment of bovine immunoprotecion against Rhipicephalus microplus using Bm86-CG antigen expressed in Pichia pastoris - (Proceedings) - (13-May-11)
Effects of pyriproxyfen and buprofezin on immature development, female oviposition, and egg-hatching in the stable fly - (Abstract Only) - (13-May-11)
Research update on the potential use of anti-tick vaccines to support the USDA Cattle Fever Tick Eradication Program - (Abstract Only) - (13-May-11)
Epidemiological and biological aspects on Ornithodoros brasiliensis (mouro tick), an argasidae tick only found on the highlands region of Rio Grande do Sul state, southern Brazil - (Abstract Only) - (13-May-11)
Proportion of White-tailed deer using medicated bait sites in Southern Texas - (Abstract Only) - (13-May-11)
Immunohistological localization of serotonin in the CNS and feeding system of the stable fly stomoxys calcitrans L. (Diptera: muscidae) - (Peer Reviewed Journal)
Liu, S.S., Li, A.Y., Witt, C.M., Perez De Leon, A.A. 2011. Immunohistological localization of serotonin in the CNS and feeding system of the stable fly stomoxys calcitrans L. (Diptera: muscidae). Archives of Insect Biochemistry and Physiology. 77(4):199-219.
Acaricide resistance of Rhipicephalus (Boophilus) microplus in State of Mato Grosso do Sul, Brazil - (Peer Reviewed Journal)
Andreotti, R., Guerrero, F., Soares, M.A., Barros, J.C., Miller, R., Perez De Leon, A.A. 2011. Acaricide resistance of Rhipicephalus (Boophilus) microplus in State of Mato Grosso do Sul, Brazil. Revista Brasileira de Parasitologia Veterinaria. 20(2):127-133.
Invasive plant-invasive insect interactions: Giant reed invasions as suitable refuge for cattle fever ticks (Acari: Ixodidae) - (Abstract Only) - (12-Dec-10)
Assessment of bacterial diversity in the cattle tick Rhipicephalus (Boophilus) microplus through tag-encoded pyrosequencing - (Peer Reviewed Journal)
Andreotti, R., Perez De Leon, A.A., Dowd, S.E., Guerrero, F., Bendele, K.G., Scoles, G.A. 2011. Assessment of bacterial diversity in the cattle tick Rhipicephalus (Boophilus) microplus through tag-encoded pyrosequencing. BMC Microbiology. 11:article 6.
Efficacy of spinosad against acaricide-resistant and -susceptible Rhipicephalus (Boophilus) microplus and acaricide-susceptible Amblyomma americanum and Dermacentor variabilis - (Peer Reviewed Journal)
Miller, R., White, W.H., Davey, R.B., George, J.E., Perez De Leon, A.A. 2011. Efficacy of spinosad against acaricide-resistant and -susceptible Rhipicephalus (Boophilus) microplus and acaricide-susceptible Amblyomma americanum and Dermacentor variabilis. Journal of Medical Entomology. 48(2):358–365.
Rhipicephalus microplus salivary gland molecules induce differential CD86 expression in murine macrophages - (Peer Reviewed Journal)
Brake, D.K., Tidwell, J.P., Wikel, S.K., Perez De Leon, A.A. 2010. Rhipicephalus microplus salivary gland molecules induce differential CD86 expression in murine macrophages. Parasites and Vectors. 3(1):Article 103.
Assessment of bacterial diversity in the cattle tick Rhipicephalus microplus through tag-encoded pyrosequencing - (Proceedings)
Andreotti, R., Perez De Leon, A.A., Dowd, S.E., Guerrero, F., Bendele, K.G., Scoles, G.A. 2011. Assessment of bacterial diversity in the cattle tick Rhipicephalus microplus through tag-encoded pyrosequencing. Acarology International Congress Proceedings. 6:11.
Efficacy and blood sera analysis of a long-acting formulation of moxidectin against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae)on treated cattle - (Peer Reviewed Journal)
Davey, R.B., Pound, J.M., Klavons, J.A., Lohmeyer, K.H., Freeman, J.M., Perez De Leon, A.A., Miller, R. 2011. Efficacy and blood sera analysis of a long-acting formulation of moxidectin against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) on treated cattle. Journal of Medical Entomology. 48(2):314-321.
Immunohistological localization of 5-HT in the CNS and feeding system of the Stable Fly - (Proceedings) - (15-Sep-10)
High resolution predictive mapping of Rhipicephalus microplus and R. annulatus in south Texas after vaccination with the anti-tick vaccine Gavac - (Abstract Only) - (23-Aug-10)
High resolution predictive mapping of Rhipicephalus microplus and R. annulatus in south Texas after vaccination with the anti-tick vaccine Gavac - (Abstract Only)
Estrada-Pena, A., Miller, R., Perez De Leon, A.A. 2010. High resolution predictive mapping of Rhipicephalus microplus and R. annulatus in south Texas after vaccination with the anti-tick vaccine Gavac [abstract]. Agricultural Research International Conference Proceedings. 1:298.
Novel approaches for control of Rhipicephalus (Boophilus) microplus - (Peer Reviewed Journal)
Temeyer, K.B., Chen, A.C., Davey, R.B., Guerrero, F., Freeman, J.M., Kammlah, D.M., Li, A.Y., Lohmeyer, K.H., Olafson, P.U., Perez De Leon, A.A., Phillips, P.L., Pound, J.M., Welch, J.B. 2012. Novel approaches for control of Rhipicephalus (Boophilus) microplus. J Tecnica Pecuaria En Mexico. 3(Suppl. 1):25-40.
Shift in rules of engagement for eradication of cattle fever ticks in the United States - (Proceedings) - (01-Jul-10)
Persistent efficacy and blood sera analysis of a long-acting (LA) formulation of moxidectin against Rhipicephalus (Boophilus) microplus on treated cattle - (Proceedings) - (17-Jun-10)
Detection of Babesia DNA in cattle fever ticks using a Reverse Line BLot - (Proceedings) - (10-Jun-10)
Evaluation of the anti-tick vaccine Gavac® against Texas outbreak strains of Rhipicephalus microplus and R. annulatus - (Abstract Only) - (15-May-10)
First report of fipronil resistance in Rhipicephalus (Boophilus) microplus of Mexico - (Peer Reviewed Journal) - (09-Apr-10)
One Health Approach to Identify Research Needs in Bovine and Human Babesioses: Workshop Report - (Peer Reviewed Journal)
Perez De Leon, A.A., Strickman, D.A., Knowles Jr, D.P., Fish, D., Thacker, E.L., De La Fuente, J., Krause, P.J., Wikel, S.K., Miller, R., Wagner, G.G., Almazan, C., Hillman, R., Messenger, M.T., Ugstad, P.O., Duhaime, R.A., Teel, P.D., Ortega-Santos, A., Hewitt, D.G., Bowers, E.J., Bent, S.J., Cochran, M.H., Mcelwain, T.F., Scoles, G.A., Suarez, C.E., Davey, R.B., Freeman, J.M., Lohmeyer, K.H., Li, A.Y., Guerrero, F., Kammlah, D.M., Phillips, P.L., Pound, J.M. 2010. One Health approach to identify research needs in bovine and human babesioses: Workshop report. Parasites & Vectors. 3:Article 36.
Current Agricultural Research Issues on One-Host ticks and Bovine Babesiosis - (Proceedings)
Perez De Leon, A.A., Knowles Jr, D.P. 2010. Current Agricultural Research Issues on One-Host ticks and Bovine Babesiosis. United States Animal Health Association Proceedings. p. 703-705.
Developing Novel Technology for the Control of Ticks of Veterinary and Human Importance - (Abstract Only) - (03-Nov-09)
Cattle Fever Ticks in the U.S.: Back to 1906? - (Abstract Only)
Perez De Leon, A.A., Pound, J.M., Kammlah, D.M., Davey, R.B., Strickman, D.A., Messenger, M., Duhaime, R., Hillman, B., George, J. 2009. Cattle Fever Ticks in the U.S.: Back to 1906?. Livestock Insect Worker's Conference Annual Meeting. June 21-23, 2009. French Lick, IN
Papular dermatitis induced in guinea pigs by the biting midge Culicoides sonorensis (Diptera: Ceratopogonidae) - (Peer Reviewed Journal)
O'Toole, D., Perez De Leon, A.A., Hearne, C., Mcholland, L.E., Yun, L., Tabachnick, W.J. 2003. Papular dermatitis induced in guinea pigs by the biting midge Culicoides sonorensis (Diptera: Ceratopogonidae). Journal of Veterinary Diagnostic Investigation. 15:67-71.

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Last Modified: 05/25/2013
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