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United States Department of Agriculture

Agricultural Research Service

Stephanie Patfield
Foodborne Toxin Detection and Prevention
Biological Science Lab Technician
stephanie.mcmahon@ars.usda.gov
Phone: (510) 559-5657
Fax: (510) 559-6429
USDA, ARS, PWA, WRRC-FTDP
800 BUCHANAN ST
ALBANY, CA, 94710


Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Detecting and discriminating among Shiga toxins - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X., Wu, V.C. 2016. Detecting and discriminating among Shiga toxins. doi: 10.1021/acs.jafc.6b00379.
Phage-mediated Shiga toxin (Stx) horizontal gene transfer and expression in non-Shiga toxigenic Enterobacter and Escherichia coli strains - (Peer Reviewed Journal)
Khalil, R., Skinner, C.B., Patfield, S.A., He, X. 2016. Phage-mediated Shiga toxin (Stx) horizontal gene transfer and expression in non-Shiga toxigenic Enterobacter and Escherichia coli strains. Pathogens and Disease. 74(5):1-11. doi: 10.1093/femspd/ftw037.
Safe, rapid, and sensitive method of quantitating and distinguishing among Shiga toxins in complex media and human serum - (Abstract Only)
Wu, V.C., Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X. 2016. Safe, rapid, and sensitive method of quantitating and distinguishing among Shiga toxins in complex media and human serum. ARS Food Safety and Inspection Service Research Workshop. ARS-FSIS.
New monoclonal antibodies against a novel subtype of Shiga toxin 1 produced by Enterobacter cloacae and their use in analysis of human serum - (Peer Reviewed Journal)
Skinner, C.B., Patfield, S.A., Khalil, R., Kong, Q., He, X. 2016. New monoclonal antibodies against a novel subtype of Shiga toxin 1 produced by Enterobacter cloacae and their use in analysis of human serum. mSphere. 1(1):e00099-15.
A new immunoassay for detecting all subtypes of Shiga toxins produced by Shiga toxin-producing E. coli in ground beef - (Peer Reviewed Journal)
He, X., Kong, Q., Patfield, S.A., Skinner, C.B., Rasooly, R. 2016. A new immunoassay for detecting all subtypes of Shiga toxins produced by Shiga toxin-producing E. coli in ground beef. PLoS One. 11(1):e0148092.
Analysis of castor by ELISAs that distinguish Ricin and Ricinus communis agglutinin (RCA) - (Peer Reviewed Journal)
Brandon, D.L., McKeon, T.A., Patfield, S.A., He, X. 2016. Analysis of castor by ELISAs that distinguish Ricin and Ricinus communis agglutinin (RCA). Journal of the American Oil Chemists' Society. 93:359-363.
Mass spectrometry-based method of detecting and distinguishing type 1 and type 2 Shiga-like toxins in human serum Reprint Icon - (Peer Reviewed Journal)
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Patfield, S.A., He, X. 2015. Mass spectrometry-based method of detecting and distinguishing type 1 and type 2 Shiga-like toxins in human serum. Toxins. 7:5236-5253.
An in vitro combined antibiotic/antibody treatment eliminates toxicity from Shiga toxin-producing E. coli Reprint Icon - (Peer Reviewed Journal)
Skinner, C.B., Patfield, S.A., He, X., Zhang, G. 2015. An in vitro combined antibiotic/antibody treatment eliminates toxicity from Shiga toxin-producing E. coli. Antimicrobial Agents and Chemotherapy. 59(9):5435-5444.
New Stx2e monoclonal antibodies for immunological detection and distinction of Stx2 subtypes Reprint Icon - (Peer Reviewed Journal)
Skinner, C.B., Patfield, S.A., Hernlem, B.J., He, X. 2015. New Stx2e monoclonal antibodies for immunological detection and distinction of Stx2 subtypes. PLoS One. 10(7):e0132419.
New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo - (Peer Reviewed Journal)
Skinner, C.B., Patfield, S.A., Stanker, L.H., Fratamico, P.M., He, X. 2014. New high-affinity monoclonal antibodies against Shiga toxin 1 facilitate the detection of hybrid Stx1/Stx2 in vivo. PLoS One. 9(6):e99854. DOI:10.1371journal.pone0099854.
Safe and effective means of detecting and quantitating Shiga-like toxins in attomole amounts Reprint Icon - (Peer Reviewed Journal)
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Dynin, I., Hui, C., Patfield, S.A., Carter, J.M., He, X. 2014. Safe and effective means of detecting and quantitating Shiga-like toxins in attomole amounts. Analytical Chemistry. 86(10):4698-4706.
Using mass spectrometry to detect and discriminate among Shiga-like toxins in the attomole range. Reprint Icon - (Abstract Only)
Silva, C.J., Erickson-Beltran, M.L., Skinner, C.B., Dynin, I.A., Hui, C., Patfield, S.A., He, X. 2014. Using mass spectrometry to detect and discriminate among Shiga-like toxins in the attomole range [abstract]. Meeting Abstract. Innovations in Mass Spectrometry. Paper No. 355.
Mouse in vivo neutralization of Escherichia coli Shiga toxin 2 with monoclonal antibodies Reprint Icon - (Peer Reviewed Journal)
Cheng, L.W., Henderson II, T.D., Patfield, S.A., Stanker, L.H., He, X. 2013. Mouse in vivo neutralization of Escherichia coli Shiga toxin 2 with monoclonal antibodies. Infection and Immunity. 5:1845-1858. DOI: 10.3390/toxins5101845.
Development of monoclonal antibodies and immunoassays for sensitive and specific detection of Shiga Toxin Stx2f - (Peer Reviewed Journal)
Skinner, C.B., Patfield, S.A., Stanker, L.H., He, X. 2013. Development of monoclonal antibodies and immunoassays for sensitive and specific detection of Shiga Toxin Stx2f. PLoS One. 8(9):e76563 DOI: 10.1371/journal/pone.0076563.
A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by escherichia coli in human and environmental samples - (Peer Reviewed Journal)
He, X., Patfield, S.A., Hnasko, R.M., Rasooly, R., Mandrell, R.E. 2013. A polyclonal antibody based immunoassay detects seven subtypes of Shiga toxin 2 produced by escherichia coli in human and environmental samples. PLoS One. 8(10):e76368.
Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2 Reprint Icon - (Peer Reviewed Journal)
Skinner, C.B., Patfield, S.A., Rasooly, R., Carter, J.M., He, X. 2013. Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2. PLoS One. 8(3):e59760.
Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk - (Peer Reviewed Journal)
He, X., Mcmahon, S.A., Skinner, C.B., Merrill, P.A., Scotcher, M., Stanker, L.H. 2013. Development and characterization of monoclonal antibodies against Shiga toxin 2 and their application for toxin detection in milk. Journal of Immunological Methods. 389(1-2):18-28. doi:10.1016/j.jim.2012.12.005.
A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli - (Peer Reviewed Journal)
He, X., Quinones, B., Mcmahon, S.A., Mandrell, R.E. 2012. A single-step purification and molecular characterization of functional Shiga toxin 2 variants from pathogenic Escherichia coli. Toxins. 4(7):487-504. doi:10.3390/toxins4070487
Evaluation and comparison of three enzyme-linked immunosorbent assay formats for the detection of ricin in milk and serum - (Peer Reviewed Journal)
He, X., Mcmahon, S.A., Rasooly, R. 2012. Evaluation and comparison of three enzyme-linked immunosorbent assay formats for the detection of ricin in milk and serum. Biocatalysis and Agricultural Biotechnology. 1(2):105-109. doi:10.1016/j.bcab.2011.08.016.
Comparison of a high-density genetic linkage map to genome features in the model grass Brachypodium distachyon - (Peer Reviewed Journal)
Huo, N., Garvin, D.F., You, F., McMahon, S.A., Luo, M., Gu, Y.Q., Lazo, G.R., Vogel, J.P. 2011. Comparison of a high-density genetic linkage map to genome features in the model grass Brachypodium distachyon. Theoretical and Applied Genetics. 123(3):455-464.
Sensitive detection of Shiga toxin 2 and some of its Stx 2 variants in environmental samples by a novel immuno-PCR assay - (Peer Reviewed Journal)
He, X., Qi, W., Quinones, B., Mcmahon, S.A., Cooley, M.B., Mandrell, R.E. 2011. Sensitive detection of Shiga toxin 2 and some of its Stx 2 variants in environmental samples by a novel immuno-PCR assay. Applied and Environmental Microbiology. 77(1):3558-3564. doi:10.1128/AEM.02205-10.
Ricin toxicokinetics and its sensitive detection in mouse sera or feces using immuno-PCR - (Peer Reviewed Journal)
He, X., Mcmahon, S.A., Henderson II, T.D., Griffey, S.M., Cheng, L.W. 2010. Ricin toxicokinetics and its sensitive detection in mouse sera or feces using immuno-PCR. PLoS One. 5(9):e12858. doi:10.1371/journal.pone.0012858.
Development of a Novel Immuno-PCR Assay for Detection of Ricin in Ground Beef, Liquid Chicken Egg and Milk - (Peer Reviewed Journal)
He, X., McMahon, S.A., McKeon, T.A., Brandon, D.L. 2010. Development of a Novel Immuno-PCR Assay for Detection of Ricin in Ground Beef, Liquid Chicken Egg and Milk. Journal of Food Protection. 73(4):695-700
Structural characterization of Brachypodium genome and its syntenic relationship with rice and wheat - (Peer Reviewed Journal)
Huo, N., Vogel, J.P., Lazo, G.R., You, F., Ma, Y., McMahon, S.A., Dvorak, J., Anderson, O.D., Luo, M., Gu, Y.Q. 2009. Structural characterization of Brachypodium genome and its syntenic relationship with rice and wheat. Plant Molecular Biology. 70: 47-61.
Last Modified: 8/27/2016
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