Projects

IMPROVING BIOCHEMICAL PROCESSES FOR THE PRODUCTION OF SUSTAINABLE FUELS AND CHEMICALS Appropriated (D)   Accession Number: 415148

NOVEL TECHNOLOGIES FOR PRODUCING RENEWABLE CHEMICALS AND POLYMERS FROM CARBOHYDRATES DERIVED FROM AGRICULTURAL FEEDSTOCKS Appropriated (D)   Accession Number: 418310

SELECTIVE DEHYDRATION OF MULTIFUNCTIONAL SUBSTRATES Specific Cooperative Agreement (S)   Accession Number: 422882

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Effects of lactic acid bacteria contamination on lignocellulosic ethanol fermentation - (Abstract Only) - (21-May-13)

Laccases from Aureobasidium pullulans - (Peer Reviewed Journal)

Rich, J.O., Leathers, T.D., Anderson, A.M., Bischoff, K.M., Manitchotpisit, P. 2013. Laccases from Aureobasidium pullulans. Enzyme and Microbial Technology. 53(1):33-37.

UV-C mutagenesis of Kluyveromyces marxianus NRRL Y-1109 for improved anaerobic growth and ethanol production at elevated temperature on biomass sugars - (Peer Reviewed Journal)

Hughes, S.R., Bang, S.S., Cox, E.J., Schoepke, A., Ochwat, K., Pinkelman, R., Nelson, D., Qureshi, N., Gibbons, W.R., Kurtzman, C.P., Bischoff, K.M., Liu, S., Cote, G.L., Rich, J.O., Jones, M.A., Cedeno, D., Doran-Peterson, J., Riano, N.M. 2013. Automated UV-C mutagenesis of Kluyveromyces marxianus NRRL Y-1109 and selection for microaerophilic growth and ethanol production at elevated temperature on biomass sugars. Journal of Laboratory Automation. DOI: 10.1177/2211068213480037.

The production of glucans via glucansucrases by Lactobacillus satsumensis isolated from a fermented beverage starter culture - (Peer Reviewed Journal)

Cote, G.L., Skory, C.D., Unser, S.M., Rich, J.O. 2012. The production of glucans via glucansucrases by Lactobacillus satsumensis isolated from a fermented beverage starter culture. Applied Microbiology and Biotechnology. DOI: 10.1007/s00253-012-4606-y.

Evaluation of laccase-mediator system (LMS) in the oxidation of veratryl alcohol - (Peer Reviewed Journal)

Larson, T.M., Anderson, A.M., Rich, J.O. 2012. Combinatorial evaluation of laccase-mediator system in the oxidation of veratryl alcohol. Biotechnology Letters. DOI: 10.1007/s10529-012-1078-1. 35(2):225-231.

Biotechnology of Aureobasidium pullulans: A phylogenetic perspective - (Abstract Only)

Manitchotpisit, P., Leathers, T.D., Peterson, S.W., Skory, C.D., Price, N.P., Rich, J.O. 2012. Biotechnology of Aureobasidium pullulans: A phylogenetic perspective [abstract]. International Congress on Yeasts. p. 194.

Increased ethanol production with UV-C mutagenized Kluyveromyces marxianus capable of anaerobic growth at elevated temperature on pentose and hexose sugars using fermentation strategies with corn pericarp hydrolysates - (Abstract Only)

Hughes, S.R., Kurtzman, C.P., Rich, J.O. 2012. Increased ethanol production with UV-C mutagenized Kluyveromyces marxianus capable of anaerobic growth at elevated temperature on pentose and hexose sugars using fermentation strategies with corn pericarp hydrolysates [abstract]. International Congress on Yeasts. IP175M.

Impact of using virginiamycin in the fuel ethanol production process on distillers grains coproducts - (Abstract Only)

Bischoff, K.M., Edrington, T.S., Rich, J.O., Zhang, Y. 2012. Impact of using virginiamycin in the fuel ethanol production process on distillers grains coproducts [abstract]. Society for Industrial Microbiology and Biotechnology. S123.

Combinatorial evaluation of the laccase-mediator system (LMS) in the oxidation of veratryl alcohol - (Abstract Only)

Larson, T.M., Anderson, A.M., Rich, J.O. 2012. Combinatorial evaluation of the laccase-mediator system (LMS) in the oxidation of veratryl alcohol [abstract]. Gordon Research Conference.

Novel antibacterial polypeptide produced by Lactobacillus paracasei strain NRRL B-50314 - (Abstract Only)

Liu, S., Wilkinson, B.J., Bischoff, K.M., Hughes, S.R., Rich, J.O., Cotta, M.A. 2012. Novel antibacterial polypeptide produced by Lactobacillus paracasei strain NRRL B-50314 [abstract]. American Society for Microbiology. Paper #2512. p. 202.

Determining the fate of virginiamycin in the fuel ethanol production process - (Abstract Only)

Bischoff, K.M., Rich, J.O., Zhang, Y. 2012. Determining the fate of virginiamycin in the fuel ethanol production process [abstract]. Fuel Ethanol Workshop. Track 3. Paper 1. p. 23.

Modification of Rhizopus lactate dehydrogenase for improved resistance to fructose 1,6-bisphosphate - (Abstract Only)

Skory, C.D., Rich, J.O. 2012. Nodification of Rhizopus lactate dehydrogenase for improved resistance to fructose 1,6-bisphosphate [abstract]. Symposium on Biotechnology for Fuels and Chemicals. Paper #9-22.

Novel antibacterial polypeptide laparaxin produced by Lactobacillus paracasei strain NRRL B-50314 via fermentation - (Peer Reviewed Journal)

Liu, S., Wilkinson, B.J., Bischoff, K.M., Hughes, S.R., Rich, J.O., Cotta, M.A. 2012. Novel antibacterial polypeptide laparaxin produced by Lactobacillus paracasei strain NRRL B-50314 via fermentation. J Pet Environ Biotechnol. 3:121. DOI: 10.4172/2157-7463.

UV-C mutagenesis of Kluyveromyces marxianus NRRL Y-1109 strain for improved anaerobic growth at elevated temperature on pentose and hexose sugars - (Abstract Only)

Hughes, S.R., Rich, J.O. 2012. UV-C mutagenesis of Kluyveromyces marxianus NRRL Y-1109 strain for improved anaerobic growth at elevated temperature on pentose and hexose sugars [abstract]. American Chemical Society.

Design and construction of a first-generation high-throughput integrated molecular biology platform for production of optimized synthetic genes and improved industrial strains - (Abstract Only)

Hughes, S.R., Butt, T.R., Bartolett, S., Riedmuller, S., Sterner, D., Farrelly, P., Bischoff, K.M., Liu, S., Rich, J.O. 2012. Design and construction of a first-generation high-throughput integrated molecular biology platform for production of optimized synthetic genes and improved industrial strains [abstract]. CBRD Advisory Board.

Development and application of automated systems for plasmid-based functional proteomics to improve syntheitc biology of engineered industrial microbes for high level expression of proteases for biofertilizer production - (Abstract Only)

Hughes, S.R., Rich, J.O., Tasaki, K., Riedmuller, S. 2012. Development and application of automated systems for plasmid-based functional proteomics to improve syntheitc biology of engineered industrial microbes for high level expression of proteases for biofertilizer production [abstract]. Society for Laboratory Automation and Screening. Track 3. Paper 4.

Production of biodiesel by transesterification of corn and soybean oils with ethanol or butanol using resin-bound truncated Candida antarctica lipase B - (Peer Reviewed Journal)

Hughes, S.R., Moser, B.R., Robinson, S., Cox, E.J., Harmsen, A.J., Friesen, J.A., Bischoff, K.M., Jones, M.A., Pinkleman, R., Bang, S.S., Tasaki, K., Doll, K.M., Qureshi, N., Liu, S., Saha, B.C., Jackson, Jr., J.S., Cotta, M.A., Rich, J.O., Caimi, P. 2012. Synthetic resin-bound truncated Candida antarctica lipase B for production of fatty acid alkyl esters by transesterification of corn and soybean oils with ethanol or butanol. Journal of Biotechnology. 159:69-77. DOI: 10.1016/j.jbiotec.2012.01.025.

VNTR fingerprinting of Kluyveromyces marxianus strains WT, 7-1, and 8-1 by using different primer types to give best results in PCR and on electrophorese gel in order to find differentiation of the DNA of the yeast strains. - (Abstract Only)

Schoepke, A., Cox, E., Rich, J.O., Hughes, S.R. 2011. VNTR fingerprinting of Kluyveromyces marxianus strains WT, 7-1, and 8-1 by using different primer types to give best results in PCR and on electrophorese gel in order to find differentiation of the DNA of the yeast strains [abstract]. American Chemical Society. Poster #11.

Cloning and expression of a beta-xylosidase from the fungus Fusarium verticillioides - (Abstract Only)

Larson, T.M., Bischoff, K.M., Rich, J.O. 2011. Cloning and expression of a beta-xylosidase from the fungus Fusarium verticillioides [abstract]. Society for Industrial Microbiology. Abstract #P23.

Adaptation of lactic acid bacteria to butanol - (Peer Reviewed Journal)

Liu, S., Bischoff, K.M., Leathers, T.D., Qureshi, N., Rich, J.O., Hughes, S.R. 2012. Adaptation of lactic acid bacteria to butanol. Biocatalysis and Agricultural Biotechnology. 1(1):57-61. DOI: http://dx.doi.org/10.1016/j.bcab.2011.08.008.

Random UV-C mutagenesis of Scheffersomyces (formerly Pichia) stipitis NRRL Y-7124 to improve anaerobic growth on lignocellulosic sugars - (Peer Reviewed Journal)

Hughes, S.R., Bischoff, K.M., Gibbons, W.R., Bang, S.S., Pinkelman, R., Slininger, P.J., Qureshi, N., Liu, S., Saha, B.C., Jackson, J.S., Cotta, M.A., Rich, J.O., Javers, J. 2012. Random UV-C mutagenesis of Scheffersomyces (formerly Pichia) stipitis NRRL Y-7124 to improve anaerobic growth on lignocellulosic sugars. Journal of Industrial Microbiology and Biotechnology. 39(1):163–173.

Bacterial contamination of commercial starch-based ethanol production - (Proceedings)

Bischoff, K.M., Leathers, T.D., Rich, J.O. 2011. Bacterial contamination of commercial starch-based ethanol production. Proceedings of the International Starch Technology Conference. p. 58-64.

High-throughput automated molecular biology platform for production of fuel ethanol yeast capable of expressing high-value heterologous proteins - (Proceedings)

Hughes, S.R., Moser, B.R., Harmsen, A.J., Bischoff, K.M., Jones, M.A., Pinkleman, R., Bang, S.S., Doll, K.M., Tasaki, K., Qureshi, N., Liu, S., Saha, B.C., Jackson, Jr, J.S., Cotta, M.A., Rich, J.O., Caimi, P. 2011. High-throughput automated molecular biology platform for production of fuel ethanol yeast capable of expressing high-value heterologous proteins. Proceedings of the Seventh International Starch Technology Conference. p. 21-29.

Purification and characterization of arabinofuranosidase from the corn endophyte Acremonium zeae - (Peer Reviewed Journal)

Bischoff, K.M., De Rezende, S.T., Larson, T.M., Liu, S., Hughes, S.R., Rich, J.O. 2011. Purification and characterization of arabinofuranosidase from the corn endophyte Acremonium zeae. Biotechnology Letters. 33(10):2013-2018. DOI: 10.1007/s10529-011-0658-9.

Bioproducts from diverse phylogenetic clades of Aureobasidium pullulans - (Abstract Only)

Manitchotpisit, P., Leathers, T.D., Peterson, S.W., Skory, C.D., Price, N.P., Rich, J.O. 2011. Bioproducts from diverse phylogenetic clades of Aureobasidium pullulans [abstract]. Asia-Pacific Biotechnology Congress and Philippine Society for Microbiology, Inc. p. 59.

Laccase production by diverse phylogenetic clades of Aureobasidium pullulans - (Peer Reviewed Journal)

Rich, J.O., Manitchotpisit, P., Peterson, S.W., Leathers, T.D. 2011. Laccase production by diverse phylogenetic clades of Aureobasidium pullulans. Rangsit Journal of Arts and Sciences. 1(1):41-47.

Exploring Lactobacillus buchneri for Biomass to Biofuels - (Abstract Only)

Liu, S., Bischoff, K.M., Leathers, T.D., Hughes, S.R., Rich, J.O. 2010. Exploring Lactobacillus buchneri for biomass to biofuels [abstract]. Symposium on Biocatalysis and Biotechnology. p. 59.

Rapid Evaluation of the Antibiotic Susceptibility of Fuel Ethanol Contaminant Biofilms - (Peer Reviewed Journal)

Rich, J.O., Leathers, T.D., Nunnally, M.S., Bischoff, K.M. 2011. Rapid evaluation of the antibiotic susceptibility of fuel ethanol contaminant biofilms. Bioresource Technology. 102(2):1124-1130.

Purification and Characterization of Two Arabinofuranosidases from the Maize Endophyte Acremonium zeae - (Abstract Only)

Bischoff, K.M., De Rezenda, S.T., Larson, T.M., Liu, S., Hughes, S.R., Rich, J.O. 2010. Purification and characterization of two Arabinofuranosidases from the maize endophyte Acremonium zeae [abstract]. Society for Industrial Microbiology. Poster No. P-12. p. 108.

Functional Expression of the Thiolase Gene thl from Clostridium beijerinckii P260 in Lactococcus lactis and Lactobacillus buchneri - (Abstract Only)

Liu, S., Bischoff, K.M., Hughes, S.R., Qureshi, N., Rich, J.O. 2010. Functional expression of the Thiolase gene THL from Clostridium beijerinckii P260 in Lactococcus lactis and Lactobacillus buchneri [abstract]. Genetics of Industrial Microorganisms. p. 36.

Lignocellulosic Biofuels: Bioenergy Research at ARS - (Abstract Only)

Rich, J.O. 2010. Lignocellulosic biofuels: Bioenergy research at ARS [abstract]. American Institute of Chemical Engineers (AIChE) Process Development Symposium. p. 13.

Production of Truncated Candida antarctica Lipase B Gene Using Automated PCR Gene Assembly Protocol and Expression in Yeast for use in Ethanol and Biodiesel Production. - (Abstract Only)

Hughes, S.R., Moser, B.R., Harmsen, A.J., Robinson, S., Bischoff, K.M., Jones, M.A., Pinkelman, R., Bang, S.B., Tasaki, K., Doll, K.M., Qureshi, N., Liu, S., Saha, B.C., Jackson Jr, J.S., Cotta, M.A., Rich, J.O., Caimi, P. 2010. Production of truncated Candida antarctica Lipase B gene using automated PCR gene assembly protocol and expression in yeast for use in ethanol and biodiesel production [abstract]. Advanced Biofuels Leadership Conference. Talk 3. p. 5.

Renewable Products Research: Applications in Polymers, Fuels, and Co-Products - (Abstract Only)

Rich, J.O., Skory, C.D., Hughes, S.R. 2010. Renewable products research: Applications in polymers, fuels, and co-products [abstract]. University of Minnesota. #1.

Production of Candida antaractica Lipase B Gene Open Reading Frame using Automated PCR Gene Assembly Protocol on Robotic Workcell & Expression in Ethanologenic Yeast for use as Resin-Bound Biocatalyst in Biodiesel Production - (Peer Reviewed Journal)

Hughes, S.R., Moser, B.R., Harmsen, A.J., Bischoff, K.M., Jones, M.A., Pinkelman, R., Bang, S.S., Tasaki, K., Doll, K.M., Qureshi, N., Liu, S., Saha, B.C., Jackson Jr, J.S., Cotta, M.A., Rich, J.O., Caimi, P. 2010. Production of Candida antaractica Lipase B gene open reading frame using automated PCR gene assembly protocol on robotic workcell and expression in ethanologenic yeast for use as resin-bound biocatalyst in biodiesel production. Journal of the Association for Laboratory Automation. 16(1):17-37. DOI: 10.1016/j.jala.2010.04.002.

Functional Expression of the Thiolase Gene thl from Clostridium beijerinckii p260 in Lactococcus lactis and Lactobacillus buchneri - (Peer Reviewed Journal)

Liu, S., Bischoff, K.M., Qureshi, N., Hughes, S.R., Rich, J.O. 2010. Functional expression of the thiolase gene THl from Clostridium beijerinckii P260 in Lactococcus lactis and Lactobacillus buchneri. New Biotechnology. 27(4):283-288.

Fermentation of Corn Fiber Hydrolysate to Lactic Acid by the Moderate Thermophile Bacillus coagulans - (Peer Reviewed Journal)

Bischoff, K.M., Liu, S., Hughes, S.R., Rich, J.O. 2010. Fermentation of corn fiber hydrolysate to lactic acid by the moderate thermophile Bacillus coagulans. Biotechnology Letters. 32:823-828.

Enhanced Production of Carboxylic Acids by Engineering of Rhizopus - (Abstract Only)

Skory, C.D., Hector, R.E., Gorsich, S.W., Rich, J.O. 2009. Enhanced Production of Carboxylic Acids by Engineering of Rhizopus [abstract].

Current Status and Future Considerations Regarding the US Biofuel Industry - (Abstract Only)

Hughes, S.R., Tasaki, K., Rich, J.O. 2009. Current status and future considerations regarding the U.S. Biofuel Industry [abstract]. Mitsubish Chemical Corporation. p. 1.

Novel Lactate Transporters from Carboxylic Acid-Producing Rhizopus - (Abstract Only)

Skory, C.D., Hector, R.E., Gorsich, S.W., Rich, J.O. 2009. Novel lactate transporters from carboxylic acid-producing Rhizopus [abstract]. United States-Japan Natural Resources Food and Agriculture Panel. p. 160-163.

Novel Lactate Transporters from Carboxylic Acid-Producing Rhizopus - (Proceedings)

Skory, C.D., Hector, R.E., Gorsich, S.W., Rich, J.O. 2009. Novel lactate transporters from carboxylic acid-producing Rhizopus. United States-Japan Cooperative Program in Natural Resources. p. 160-163.

Production of the Anaerobic GMAX-L Yeast Using High-Throughput Mating and Transformation of Saccharomyces cerevisiae With Identified Genes For Simultaneous Cellulosic Ethanol and Biodiesel Production - (Abstract Only)

Hughes, S.R., Rich, J.O., Moser, B.R., Doll, K.M., Bischoff, K.M., Qureshi, N., Jones, M., Tasaki, K. 2009. Production of the anaerobic GMAX-L yeast using high-throughput mating and transformation of Saccharomyces cerevisiae with identified genes For simultaneous cellulosic ethanol and biodiesel production [abstract]. Bradley Biology Chemisty Seminar Series.

Lactic Acid Bacteria – Friend or Foe? Lactic Acid Bacteria in the Production of Polysaccharides and Fuel Ethanol - (Peer Reviewed Journal)

Rich, J.O., Bischoff, K.M., Leathers, T.D., Cote, G.L., Liu, S. 2010. Lactic acid bacteria – Friend or Foe? Lactic acid bacteria in the production of Polysaccharides and fuel ethanol. KKU Research Journal. 15(5):424-435.

Lactic Acid Bateria - Friend or Foe? Lactic Acid Bacteria in the Production of Polysaccharides and Fuel Ethanol - (Abstract Only)

Rich, J.O., Liu, S., Leathers, T.D., Bischoff, K.M. 2009. Lactic acid bacteria - Friend or Foe? Lactic acid bacteria in the production of polysaccharides and fuel ethanol [abstract]. Conference on Lactic Acid Bacteria in Thai Food and Feed Industries. Paper L01. p. 69.

Genetic Engineering of Rhizopus for Enhancing Lactic Acid Production - (Abstract Only)

Rich, J.O., Skory, C.D. 2009. Genetic Engineering of Rhizopus for enhancing lactic acid production [abstract]. Conference on Fermentation Technology for Value Added Agricultural Products. Paper Fer406. p. 25.

Report membrane transport of lactic acid in the filamentous fungus Rhizopus - (Peer Reviewed Journal)

Skory, C.D., Hector, R.E., Gorsich, S.W., Rich, J.O. 2009. Report membrane transport of lactic acid in the filamentous fungus Rhizopus. KKU Research Journal. 15(9):826-831.

Analysis of a Functional Lactate Permease in the Fungus Rhizopus - (Peer Reviewed Journal)

Skory, C.D., Hector, R.E., Gorsich, S., Rich, J.O. 2010. Analysis of a functional lactate permease in the fungus Rhizopus. Enzyme and Microbial Technology. 46(1):43-50.

Industrial Saccharomyces cerevisiae Yeast Strain Engineered to Convert Glucose, Mannose, Arabinose, and Xylose (GMAX) to Ethanol Anaerobically - (Abstract Only)

Hughes, S.R., Qureshi, N., Butt, T., Bang, S., Bischoff, K.M., Saha, B.C., Liu, S., Rich, J.O. 2009. Industrial Saccharomyces cerevisiae yeast strain engineered to convert glucose, mannose, arabinose, and xylose (GMAX) to ethanol anaerobically [abstract]. American Institute of Chemical Engineers. Talk #2. p. 1.

Rapid evaluation of fuel ethanol contaminant biofilms - (Abstract Only)

Rich, J.O., Leathers, T.D., Bischoff, K.M. 2009. Rapid Evaluation of Fuel Ethanol Contaminant Biofilms [abstract]. 31st Symposium on Biotechnology for Fuels and Chemicals.

Hemicellulolytic Enzymes from the Maize Endophyte Acremonium zeae - (Abstract Only)

Bischoff, K.M., Jordan, D.B., De Rezende, S.T., Rich, J.O. 2009. Hemicellulolytic enzymes from the maize endophyte Acremonium zeae [abstract]. Biotechnology for Fuels and Chemicals. Poster No. 5-57. p. 120.

Application of combinatorial biocatalysis for a unique ring expansion of dihydroxymethylzearalenone - (Peer Reviewed Journal)

Rich, J.O., Budde, C.L., Mcconeghey, L.D., Cotterill, I.C., Mozhaev, V.V., Singh, S.B., Goetz, M.A., Zhao, A., Michels, P.C., Khmelnitsky, Y.L. 2009. Application of combinatorial biocatalysis for a unique ring expansion of dihydroxymethylzearalenone. Bioorganic and Medicinal Chemistry Letters. 19:3059-3062.

Automated High Throughput GMAX-L Strains of Saccharomyces Cereviciae for Profitable Cellulosic Ethanol Production from Industrial Hydrosylates - (Abstract Only)

Hughes, S.R., Tasaki, K., Doll, K.M., Moser, B.R., Rich, J.O., Qureshi, N., Hector, R.E., Dien, B.S., Cotta, M.A., Bischoff, K.M., Liu, S., Bang, S., Jones, M. 2009. Automated high throughput GMAX-L strains of Saccharomyces cereviciae for profitable cellulosic ethanol production from industrial hydrosylates [abstract]. Society for In-Vitro Biology. p. 122.

Automated Yeast Transformation Protocol to Engineer S. cerevisiae Strains for Cellulosic Ethanol Production with Open Reading Frames that Express Proteins Binding to Xylose Isomerase Identified using Robotic Two-hybrid Screen - (Peer Reviewed Journal)

Hughes, S.R., Rich, J.O., Bischoff, K.M., Hector, R.E., Qureshi, N., Saha, B.C., Dien, B.S., Liu, S., Jackson Jr, J.S., Sterner, D.E., Butt, T.R., Labaer, J., Cotta, M.A. 2009. Automated yeast transformation protocol to engineer S. cerevisiae strains for cellulosic ethanol production with open reading frames that express proteins binding to xylose isomerase identified using robotic two-hybrid screen. Journal of the Association for Laboratory Automation. 8:200-212.

Novel Oxidation of Cyclosporin A: Preparation of Cyclosporin Methyl Vinyl Ketone (Cs-MVK) - (Peer Reviewed Journal)

Yang, Z., Pattamana, K., Molino, B.F., Haydar, S.N., Cao, Y., Bois, F., Maeng, J., Hemenway, M.S., Rich, J.O., Khmelnitsky, Y.L., Friedrich, T., Peace, D., Michels, P. 2009. Novel oxidation of Cyclosporin A: Preparation of Cyclosporin methyl vinyl ketone (Cs-MVK). Synlett. 18:2935-2938.

Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings - (Abstract Only)

Hughes, S.R., Doll, K., Moser, B., Bang, S., Rich, J., Qureshi, N., Hector, R., Dien, B., Cotta, M., Bischoff, K., Liu, S., Jones, M. 2009. Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings [abstract]. LabAutomation 2009. Abstract No. 109. p. 42.

Automated Yeast Mating Protocol Using Open Reading Frames from Saccharomyces cerevisiae Genome to Improve Yeast Strains for Cellulosic Ethanol Production - (Peer Reviewed Journal)

Hughes, S.R., Hector, R.E., Rich, J.O., Qureshi, N., Bischoff, K.M., Dien, B.S., Saha, B.C., Liu, S., Jackson Jr, J.S., Sterner, D.E., Butt, T.R., Labaer, J., Cotta, M.A. 2009. Automated yeast mating protocol using open reading frames from Saccharomyces cerevisiae genome to improve yeast strains for cellulosic ethanol production. Journal of the Association for Laboratory Automation. 8:190-199.

Extracellular Hemicellulolytic Enzymes from the Maize Endophyte Acremonium zeae - (Peer Reviewed Journal)

Bischoff, K.M., Wicklow, D.T., Jordan, D.B., De Rezende, S.T., Liu, S., Hughes, S.R., Rich, J.O. 2009. Extracellular hemicellulolytic enzymes from the maize endophyte Acremonium zeae. Current Microbiology. 58:499-503.

Modeling Bacterial Contamination of Fuel Ethanol Fermentation - (Peer Reviewed Journal)

Bischoff, K.M., Liu, S., Leathers, T.D., Worthington, R.E., Rich, J.O. 2009. Modeling bacterial contamination of fuel ethanol fermentation. Biotechnology and Bioengineering. 103(1):117-122.

Make use of lactic acid bacteria in biomass to biofuel - (Abstract Only)

Liu, S., Bischoff, K.M., Leathers, T.D., Hughes, S.R., Rich, J.O. 2008. Make use of lactic acid bacteria in biomass to biofuel [abstract]. International Symposium on Biocatalysis and Biotechnology. Post No. M-2. p. 60.

Conversion of Biomass Hydrolysates and Other Substrates to Ethanol and Other Chemicals by Lactobacillus buchneri - (Peer Reviewed Journal)

Liu, S., Bischoff, K.M., Hughes, S.R., Leathers, T.D., Price, N.P., Qureshi, N., Rich, J.O. 2009. Conversion of biomass hydrolysates and other substrates to ethanol and other chemicals by Lactobacillus buchneri. Letters of Applied Microbiology. 48(3):337-342.

Three-vector System for use on Automated Systems for High-level Functional Expression of Value-added Co-products with Xylose Isomerase and Xylulokinase in an Industrial Saccharomyces cerevisiae Strain - (Abstract Only)

Rich, J.O., Hughes, S.R., Bischoff, K.M., Leathers, T.D. 2008. Three-vector system for use on automated systems for high-level functional expression of value-added co-products with Xylose Isomerase and Xylulokinase in an industrial Saccharomyces cerevisiae strain [abstract]. Indiana Council of Engineering Societies. Abstract 13. p. 4.

Inhibition of Rhizopus Lactate Dehydrogenase by Fructose 1,6-bisphosphate - (Peer Reviewed Journal)

Skory, C.D., Mertens, J.A., Rich, J.O. 2009. Inhibition of Rhizopus lactate dehydrogenase by fructose 1,6-bisphosphate. Enzyme and Microbial Technology. 44(4):242-247.

Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system - (Peer Reviewed Journal)

Hughes, S.R., Sterner, D.E., Bischoff, K.M., Hector, R.E., Dowd, P.F., Qureshi, N., Bang, S.S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Dien, B.S., Mertens, J.A., Caughey, R.J., Liu, S., Butt, T.R., Labaer, J., Cotta, M.A., Rich, J.O. 2009. Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system. Plasmid Journal. 61(1):22-38.

Improving Biocatalysts for the Production of Biofuels from Lignocellulosic Feedstocks - (Abstract Only)

Rich, J.O., Hughes, S.R., Bischoff, K.M., Leathers, T.D., Liu, S. 2008. Improving biocatalysts for the production of biofuels from Lignocellulosic feedstocks [abstract]. U.S. Japan Natural Resources Meeting. p. 54-55.

Cellulosic Ethanol Production from Saccharomyces cerevisiae Engineered for Anaerobic Conversion of Pretreated Lignocellulosic Sugars to Ethanol - (Abstract Only)

Rich, J.O., Bischoff, K.M., Hughes, S.R. 2008. Cellulosic ethanol production from Saccharomyces cerevisiae engineered for anaerobic conversion of pretreated Lignocellulosic sugars to ethanol [abstract]. American Chemical Society. Paper ID1211547.

Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars - (Abstract Only)

Hughes, S.R., Javers, J., Labaer, J., Butt, T., Hector, R.E., Dien, B.S., Saha, B.C., Bischoff, K.M., Kohl, S., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars [abstract]. Society of Industrial Microbiology. Session 34, S164, p. 106.

Proteomic Approach for Molecular Mechanisms under Ethanol Stress in Lactobacillus buchneri - (Abstract Only)

Liu, S., Bischoff, K.M., Leathers, T.D., Hughes, S.R., Rich, J.O. 2008. Proteomic approach for molecular mechanisms under ethanol stress in Lactobacillus buchneri [abstract]. American Society for Microbiology. 0-030. p. 155.

Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic sugars anaerobically to ethanol - (Abstract Only)

Hughes, S.R., Bischoff, K.M., Hector, R.E., Dien, B.S., Saha, B.C., Javers, J., Gibbons, W., Bang, S., Nelson, R., Standish, K., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae engineered to convert pretreated Lignocellulosic sugars anaerobically to ethanol [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit, Seminar 6. p. 1.

Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain - (Abstract Only)

Hughes, S.R., Jackson Jr, J.S., Dowd, P.F., Hector, R.E., Bischoff, K.M., Sterner, D., Bang, S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Li, X., Caughey, R.J., Liu, S., Skory, C.D., Butt, T., Labaer, J., Dien, B.S., Saha, B.C., Bowen, M., Qureshi, N., Rich, J.O., Leathers, T.D., Cotta, M.A., Farrelly, P., Slininger P.J. 2008. Three-vector system for high-level functional expression of value-added co-products with Xylose Isomerase and Xylulokinase in an industrial Saccharomyces cerevisiae strain [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit. Poster #1. p. 4.

Ethanol production from modern biorefinery: Robotic platform for production of Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic sugars to ethanol anaerobically - (Abstract Only)

Hughes, S.R., Rich, J.O. 2008. Ethanol production from modern biorefinery: Robotic platform for production of Saccharomyces cerevisiae engineered to convert pretreated Lignocellulosic sugars to ethanol anaerobically [abstract]. Laboratory Robotics Information Group (LRIG). Seminar 1. p. 1.

Reversible derivatization to enhance enzymatic synthesis: Chemoenzymatic synthesis of Doxorubicin-14-O-Esters - (Peer Reviewed Journal)

Cotterill, I.C., Rich, J.O., Scholten, M.W., Mozhaeva, L., Michels, P.C. 2008. Reversible derivatization to enhance enzymatic synthesis: Chemoenzymatic synthesis of Doxorubicin-14-O-Esters. Biotechnology and Bioengineering. 101(3):435-440.

Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain - (Peer Reviewed Journal)

Hughes, S.R., Dowd, P.F., Hector, R.E., Panavas, T., Sterner, D.E., Qureshi, N., Bischoff, K.M., Bang, S.B., Mertens, J.A., Johnson, E.T., Li, X., Jackson Jr, J.S., Caughey, R.J., Riedmuller, S.B., Bartolett, S., Liu, S., Rich, J.O., Farrelly, P.J., Butt, T.R., Labaer, J., Cotta, M.A. 2008. Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain. Journal of Peptide Science. 14(9):1039-1050. Available: http://www3.interscience.wiley.com/cgi-bin/fulltext/119030240/PDFSTART.

Regulatory concerns associated with use of value-added recombinant proteins and peptides screened in hgh-throughput for expression in fuel ethanol yeast strains - (Abstract Only)

Hughes, S.R., Dowd, P.F., Hector, R.E., Bischoff, K.M., Rich, J.O., Cotta, M.A., Slininger, P.J., Rosentrater, K.A. 2008. Regulatory concerns associated with use of value-added recombinant proteins and peptides screened in hgh-throughput for expression in fuel ethanol yeast strains [abstract]. LabAutomation 2008, Track 5, Seminar 4. p. 5.

Regulatory steps associated with use of value-added recombinant proteins and peptides screened in high-throughput for expression in genetically engineered starch and cellulosic fuel ethanol yeast strains - (Abstract Only)

Hughes, S.R., Chen, H., Tsai, D., Thompson, W., Dowd, P.F., Hooberman, B., Benjamin, L., Alexander, L.J., Rosentrater, K.A., Rich, J.O. 2007. Regulatory steps associated with use of value-added recombinant proteins and peptides screened in high-throughput for expression in genetically engineered starch and cellulosic fuel ethanol yeast strains [abstract]. FDA Meeting. Talk 1.

High-Throughput Fully Automated Construction of a Multiplex Library of Mutagenized Open Reading Frames for an Insecticidal Peptide Using a Plasmid-Based Functional Proteomic Robotic Workcell with Improved Vacuum System - (Peer Reviewed Journal)

Hughes, S.R., Dowd, P.F., Hector, R.E., Riedmuller, S.B., Bartolett, S., Mertens, J.A., Qureshi, N., Liu, S., Bischoff, K.M., Li, X., Jackson Jr, J.S., Sterner, D., Panavas, T., Cotta, M.A., Farrelly, P.J., Butt, T. 2007. High-throughput fully automated construction of a multiplex library of mutagenized open reading frames for an insecticidal peptide using a plasmid-based functional proteomic robotic workcell with improved vacuum system. Journal of Laboratory Automation. 12(4):202-212.