Projects

DEVELOP ENHANCED PHYTASES FOR ANIMAL FEED AND FOR INCORPORATION INTO NEW PLANT CULTIVARS REQUIRING LESS PHOSPHORUS FERTILIZERS Appropriated (D)   Accession Number: 418203

Publications

Impact of Assay conditions on activity estimate and kinetics comparison of Aspergillus niger PhyA and Escherichia coli AppA2 phytases - (Peer Reviewed Journal)

Weaver, J.D., Ullah, A.H., Sethumadhavan, K., Mullaney, E.J., Lei, X.G. 2009. Comparisons of activity assay methods and kinetics of Aspergillus niger PhyA and Escherichia coli AppA2 phytases. Journal of Agriculture and Food Chemistry. 57:5315-5320.

Unfolding and Refolding of Aspergillus Niger PhyB Phytase: Role of Disulfide Bridges - (Peer Reviewed Journal)

Ullah, A.H., Sethumadhavan, K., Mullaney, E.J. 2008. Unfolding and Refolding of Aspergillus Niger PhyB Phytase: Role of Disulfide Bridges. Journal of Agriculture and Food Chemistry. 56(17):8179-8183.

Kinetic Characterization of O-Phospho-L-Tyrosine Phosphohydrolase Activity of Two Fungal Phytases. - (Peer Reviewed Journal)

Ullah, A.H., Sethumadhavan, K., Mullaney, E.J. 2008. Kinetic characterization of o-phospho-L-tyrosine phosphohydrolase activity of two fungal phytases. Journal of Agriculture and Food Chemistry. 56(16):7467-7471.

Salt Effect on the pH Profile and Kinetic Parameters of Microbial Phytases - (Peer Reviewed Journal)

Ullah, A.H., Sethumadhavan, K., Mullaney, E.J. 2008. Salt effect on the pH profile and kinetic parameters of microbial phytases. Journal of Agricultural and Food Chemistry. 56:3398-3402.

OPTIMIZING THE PH PROFILE OF FUNGAL PHYTASE THROUGH PROTEIN ENGINEERING ALSO ENHANCES ACTIVITY - (Abstract)

Mullaney, E.J., Ullah, A.H., Sethumadhavan, K., Lei2, X. 2007. Optimizing the ph profile of fungal phytase through protein engineering also enhances activity (abstract). Society of Industrial Microbiology Annual Meeting. p. 146.

Altering the Substrate Specificity Site of Aspergillus Niger PhyB shifts the pH optimum to pH 3.2 - (Peer Reviewed Journal)

Weaver, J.D., Mullaney, E.J., Lei, X.G. 2007. Altering the substrate specificity site of aspergillus niger PhyB shifts the pH optimum to pH 3.2. Applied Microbiology and Biotechnology. (2007)76:117-122.

Enzymatic Comparisons of Aspergillus niger PhyA and Escherichia coli AppA2 Phytases - (Abstract)

Weaver, J.D., Ullah, A.H., Sethumadhavan, K., Mullaney, E.J., Lei, X.G. 2007. Enzymatic comparisons of Aspergillus Niger PhyA and Escherichia Coli AppA2 Phytases (abstract). Journal of Animal Science. 85(Supplement 1):647.

ADOPTING SELECTED HYDROGEN BONDING AND IONIC INTERACTIONS FROM ASPERGILLUS FUMIGATUS PHYTASE STRUCTURE IMPROVES THE THERMOSTABILITY OF ASPERGILLUS NIGER PHYA PHYTASE - (Peer Reviewed Journal)

Zhang, W., Mullaney, E.J., Lei, X. 2007. Adopting selected hydrogen bonding and ionic interactions from Aspergillus fumigatus phytase structure improves the thermostability of Aspergillus niger PhyA phytase. Applied and Environmental Microbiology. 73(9):3069-3076.

SHIFTING THE PH PROFILE OF ASPERGILLUS NIGER PHYA PHYTASE TO MATCH THE STOMACH PH ENHANCES ITS EFFECTIVENESS AS AN ANIMAL FEED ADDITIVE - (Peer Reviewed Journal)

Kim, T., Mullaney, E.J., Porres, J.M., Roneker, K.R., Crowe, S., Rice, S., Ko, T., Ullah, A.H., Daly, C.B., Welch, R.M., Lei, X.G. 2006. Shifting the ph profile of Aspergillus niger PhyA phytase to match the stomach ph enhances its effectiveness as an animal feed additive. Applied and Environmental Microbiology. 72(6):4397-4403.

PHYTASE: SOURCE, STRUCTURE, AND APPLICATION - (Book/Chapter)

Lei, X. G., Porres, J. M., Mullaney, E. J. and Brinch-Pedersen, H. 2007. Phytase: source, structure and application. In: Industrial Enzymes, structure, function and applications. Editied by Polina, J. and MacCabe, A. P. Springer, Dordrecht, The Netherlands, p. 505-529.

CONSERVATION OF AN EIGHT-CYSTEINE MOTIF IN FUNGAL HISTIDINE ACID PHOSPHATASES - (Abstract)

Mullaney, E.J., Ullah, A.H. 2005. Conservation of an eight-cysteine motif in fungal histidine acid phosphatases (abstract). Mycological Society of America. p. 169.

PHYTASES: ATTRIBUTES, CATALYTIC MECHANISMS AND APPLICATIONS - (Abstract)

Mullaney, E.J., Ullah, A.H. 2005. Phytases: attributes, catalytic mechanisms and applications (abstract). Inositol Phosphates in the Plant and Soil System. p.17-18.

MONITORING OF UNFOLDING AND REFOLDING IN FUNGAL PHYTASE (PHYA) BY DYNAMIC LIGHT SCATTERING - (Peer Reviewed Journal)

Ullah, A.H., Sethumadhavan, K., Mullaney, E.J. 2005. Monitoring of unfolding and refolding in fungal phytase (phyA) by dynamic light scattering. Biochemical and Biophysical Research Communications. 327(4):993-998.

CONSERVATION OF CYSTEINE RESIDUES IN FUNGAL HISTIDINE ACID PHYTASES - (Peer Reviewed Journal)

Mullaney, E.J., Ullah, A.H., 2005. Conservation of cysteine residues in fungal histidine acid phytases. Biochemical and Biophysical Research Communications. Biochemical and Biophysical Research Communications. 328(2):404-408.

ENZYMES WITH PHYTASE ACTIVITY - (Abstract)

Mullaney, E.J. 2004. Enzymes with phytase activity(abstract). American Oil Chemists Society. p.118

THE TERM PHYTASE COMPRISES SEVERAL DIFFERENT CLASSES OF ENZYMES - (Peer Reviewed Journal)

Mullaney, E.J., Ullah, A.H. 2003. Phytases comprises structurally different classes of enzymes. Biochemical and Biophysical Research Communications. 312(1):179-184.

FUNGAL PHYA GENE EXPRESSED IN POTATO LEAVES PRODUCES ACTIVE AND STABLE PHYTASE - (Peer Reviewed Journal)

Ullah, A.H., Sethumadhavan, K., Mullaney, E.J., Ziegelhoffer, T., Austin-Phillips, S. 2003. Fungal phyA gene expressed in potato leaves produces active and stable phytase. Biochemical and Biophysical Research Communications. 306(2):603-609.

CONSERVED STRUCTURAL FEATURES OF A PUTATIVE HISTIDINE ACID PHOSPHATASE ENCODED BY A NEUROSPORA CRASSA GENE. - (Proceedings/Symposium)

Mullaney, E.J., Daly, C.B. 2003. Conserved structural features of a putative histidine acid phosphatase encoded by a neurospora crassa gene. In: Proceedings of the Joint meeting of the Mycological Society of America and the British Mycological Society annual meeting, July 27-31, 2003, Monterey, CA. p. 75.

CONSERVATION OF ACTIVE SITE AND ESSENTIAL STRUCTURAL COMPONENTS ENCODED BY A PUTATIVE NEUROSPORA CRASSA PHYTASE GENE - (Proceedings/Symposium)

Mullaney, E.J., Daly, C.B. 2003. Conservation of active site and essential structural components encoded by a putative neurospora crassa phytase gene. In: Proceedings of the Fungal Genetics Conference, March 18-23, 2003, Pacific Grove, CA. p. 75.

ALTERATION OF THE PH VERSUS ACTIVITY PROFILE OF FUNGAL PHYTASE BY SITE-DIRECTED MUTAGENESIS - (Abstract)

Ullah, A.H., Mullaney, E.J. 2003. Alteration of the ph versus activity profile of fungal phytase by site-directed mutagenesis (abstract). European Symposium of the Protein Society.

MOLECULAR METHODS FOR IDENTIFICATION OF PLANT PATHOGENIC FUNGI - (Book/Chapter)

Klich, M.A., Mullaney, E.J. 2004. Molecular methods for identification of plant pathogenic fungi. In: Arora, D.K., Bridge, P.D., Bhatnagar, D., editors. Fungal Biotechnology in Agricultural, Food and Environmental Applications. New York, NY: Marcel Dekker. p. 49-55.

STRUCTURAL REFINEMENT OF FUNGAL PHYTASE (PHY A)FOR ALTERATION OF PH OPTIMUM - (Abstract)

Ullah, A.H., Mullaney, E.J. 2002. Structural refinement of fungal phytase (phy a)for alteration of ph optimum (Abstract). Protein Structure.