Page Banner

United States Department of Agriculture

Agricultural Research Service

Related Topics

Steven Joseph Klosterman
Crop Improvement and Protection Research
Research Molecular Biologist

Phone: (831) 755-2845
Fax: (831) 755-2814

1636 E ALISAL ST
SALINAS, CA, 93905

Projects
Detection and Management of Pathogens in Strawberry and Vegetable Production Systems
Appropriated (D)
  Accession Number: 426096
Development of a Pathogen Detection Early Warning System for Spinach Downy Mildew
Trust (T)
  Accession Number: 424554

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Coupling spore traps and quantitative PCR assays for detection of the downy mildew pathogens of spinach (Peronospora effusa) and beet (Peronospora schachtii) - (Peer Reviewed Journal)
Klosterman, S.J., Anchieta, A.G., Mcroberts, N., Koike, S.T., Subbarao, K.V., Voglmayr, H., Choi, Y., Thines, M., Martin, F.N. 2014. Coupling spore traps and quantitative PCR assays for detection of the downy mildew pathogens of spinach (Peronospora effusa) and beet (Peronospora schachtii). Phytopathology. DOI: 10.1094/PHYTO-02-14-0054-R.
Deep mRNA sequencing reveals stage-specific transcriptome alterations during microsclerotia development in the smoke tree vascular wilt pathogen, Verticillium dahliae - (Peer Reviewed Journal)
Xiong, D., Wang, Y., Ma, J., Klosterman, S.J., Xiao, S., Tian, C. 2014. Deep mRNA sequencing reveals stage-specific transcriptome alterations during microsclerotia development in the smoke tree vascular wilt pathogen, Verticillium dahliae. BMC Genomics. 15:324.
RNA-seq analyses of gene expression in the microsclerotia of Verticillium dahliae - (Peer Reviewed Journal)
Duressa, D., Anchieta, A.G., Chen, D., Klimes, A., Garcia-Pedrajas, M.D., Dobinson, K.F., Klosterman, S.J. 2013. RNA-seq analyses of gene expression in the microsclerotia of Verticillium dahliae. BMC Genomics. doi: 10.1186/1471-2164-14-607.
High throughput analysis of gene expression in microsclerotia of Verticillium dahliae - (Abstract Only)
Klosterman, S.J., Duressa, D., Anchieta, A.G., Chen, D., Klimes, A., Garcia-Pedrajas, M.D., Dobinson, K.F. 2013. High throughput analysis of gene expression in microsclerotia of Verticillium dahliae. In: Koopmann, B., von Tiedemann, A., editors. Proceedings of the 11th International Verticillium Symposium. German Phytomedical Society, DPG-Verlag. p. 32.
Real-time PCR and spore trap-based detection of the downy mildew pathogen, Peronospora effusa - (Abstract Only)
Klosterman, S.J., McRoberts, N., Koike, S.T., Subbarao, K.V., Voglmayr, H., Thines, M., Martin, F.N. 2013. Real-time PCR and spore trap-based detection of the downy mildew pathogen, Peronospora effusa. Acta Phytopathologica Sinica. 43 (supplement):297.
Detection and quantification of Verticillium dahliae in spinach seed - (Abstract Only)
Duressa, D., Rauscher, G.M., Koike, S.T., Mou, B., Hayes, R.J., Maruthachalam, K., Subbarao, K.V., Klosterman, S.J. 2013. Detection and quantification of Verticillium dahliae in spinach seed. Acta Phytopathologica Sinica. 43 (supplement):245.
RNA-seq analyses of gene expression in the microsclerotia of Verticillium dahliae - (Abstract Only)
Duressa, D., Anchieta, A.G., Chen, D., Klimes, A., Dobinson, K.F., Garcia-Pedrajas, M., Klosterman, S.J. 2013. RNA-seq analyses of gene expression in the microsclerotia of Verticillium dahliae. Fungal Genetics Conference Proceedings. p. 194.
Colonization of spinach by Verticillium dahliae and effects of pathogen localization on the efficacy of seed treatments - (Peer Reviewed Journal)
Maruthachalam, K., Klosterman, S.J., Anchieta, A.G., Mou, B., Subbarao, K.V. 2013. Colonization of spinach by Verticillium dahliae and effects of pathogen localization on the efficacy of seed treatments. Phytopathology. 103:268-280.
Transposable elements in phytopathogenic Verticillium spp.: insights into genome evolution and inter- and intra-specific diversification - (Peer Reviewed Journal)
Amyotte, S.G., Tan, X., Pennerman, K., Del Mar Jimenez-Gasco, M., Klosterman, S.J., Ma, L., Dobinson, K.F., Veronese, P. 2012. Transposable elements in phytopathogenic Verticillium spp.: insights into genome evolution and inter- and intra-specific diversification. Biomed Central (BMC) Genomics. 13:314.
A real-time PCR assay for detection and quantification of verticillium dahliae in spinach seed - (Peer Reviewed Journal)
Duressa, D.O., Rauscher, G.M., Koike, S.T., Mou, B., Hayes, R.J., Maruthachalam, K., Subbarao, K.V., Klosterman, S.J. 2012. A real-time PCR assay for detection and quantification of verticillium dahliae in spinach seed. Phytopathology. 102:443-451.
Selection for resistance to Verticillium wilt caused by race 2 isolates of Verticillium dahliae in accessions of lettuce (Lactuca sativa L.). - (Peer Reviewed Journal)
Hayes, R.J., Maruthachalam, K., Vallad, G.E., Klosterman, S.J., Subbarao, K.V. 2011. Selection for resistance to Verticillium wilt caused by race 2 isolates of Verticillium dahliae in accessions of lettuce (Lactuca sativa L.).HortScience. 46(2):201-206.
Analysis of Verticillium dahliae suggests a lack of correlation between genotypic diversity and virulence phenotypes. - (Peer Reviewed Journal)
Atallah, Z.K., Maruthachalam, K.K., Vallad, G.E., Davis, M., Klosterman, S.J., Subbarao, K.V. 2011. Analysis of Verticillium dahliae suggests a lack of correlation between genotypic diversity and virulence phenotypes. Plant Disease. 95:1224-1232.
Verticillium comparative genomics yields insights into niche adaptation by plant vascular wilt pathogens. - (Abstract Only)
Klosterman, S.J., Subbarao, K.V., Kang, S., Veronese, P., Gold, S.E., Thomma, B., Chen, Z., Henrissat, B., Lee, Y., Park, J., Garcia-Pedrajas, M.D., Barbara, D., Anchieta, A.G., Jonge, R., Santhanam, P., Maruthachalam, K., Atallah, Z.K., Amyotte, S., Paz, Z., Inderbitzin, P., Hayes, R.J., Heiman, D., Young, S., Zeng, Q., Engels, R., Galagan, J., Cuomo, C., Dobinson, K.F., Ma, L. 2011. Verticillium comparative genomics yields insights into niche adaptation by plant vascular wilt pathogens. Phytopathology. 101:S208.
Colonization of spinach (Spinacia oleracea L.) by GFP-tagged verticillium dahliae. - (Abstract Only)
Maruthachalam, K., Klosterman, S.J., Subbarao, K.V. 2011. Colonization of spinach (Spinacia oleracea L.) by GFP-tagged verticillium dahliae. Phytopathology. 101: S115.
Development of a qPCR assay for quantification of verticillium dahliae in spinach seed. - (Abstract Only)
Duressa, D.O., Rauscher, G.M., Mou, B., Hayes, R.J., Koike, S.T., Maruthachalam, K., Subbarao, K.V., Klosterman, S.J. 2011. Development of a qPCR assay for quantification of verticillium dahliae in spinach seed.Phytopathology. 101:S46.
SSH reveals a linkage between a senescence-associated protease and Verticillium wilt symptom development in lettuce (Lactuca sativa) - (Peer Reviewed Journal)
Klosterman, S.J., Anchieta, A.G., Garcia-Pedrajas, M.D., Maruthachalam, K., Hayes, R.J., Subbarao, K.V. 2011. SSH reveals a linkage between a senescence-associated protease and Verticillium wilt symptom development in lettuce (Lactuca sativa). Physiological and Molecular Plant Pathology. 76:48-58.
Comparative genomics yields insights into niche adaptation of plant vascular wilt pathogens. Reprint Icon - (Peer Reviewed Journal)
Klosterman, S.J., Subbarao, K.V., Kang, S., Veronese, P., Gold, S.E., Thomma, B.P.H.J, Chen, Z., Henrissat, B., Lee, Y., Park, J., Garcia-Pedrajas, M.D., Dez, B.J., Anchieta, A.G., De Jonge, R., Parthasarathy, S., Maruthachalam, K., Atallah, Z.K., Amyotte, S., Paz, Z., Inderbitizin, P., Hayes, R.J., Heiman, D.I., Young, S., Zeng, Q., Engels, R., Galagan, J., Cuomo, C., Dobinson, K.F., Ma, L-J. 2011. Comparative genomics yields insights into niche adaptation of plant vascular wilt pathogens. PLoS Pathogens. 7(7): e1002137.
The inheritance of resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante. Reprint Icon - (Peer Reviewed Journal)
Hayes, R.J., Mchale, L.K., Vallad, G.E., Jose-Truco, M., Michelmore, R.W., Klosterman, S.J., Maruthachalam, K., Subbarao, K.V. 2011. The inheritance of resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae in the lettuce cultivar La Brillante. Theoretical and Applied Genetics. DOI 10.1007/s00122-011-1603-y.
One Step Construction of Agrobacterium Recombination-ready-plasmids (OSCAR), an efficient and robust tool for ATMT based gene deletion construction in fungi - (Peer Reviewed Journal)
Zahi, P., Garcia-Pedrajas, M., Andrews, D.L., Klosterman, S.J., Montanez, B., Gold, S.E. 2011. One step construction of agrobacterium recombination-ready-plasmids (OSCAR), an efficient and robust tool for ATMT based gene deletion construction in fungi. Fungal Genetics and Biology. 48:677-684.
One step construction of agrobacterium recombination-ready plasmids (OSCAR), an efficient and robust tool for ATMT based gene deletion construction in fungi. - (Peer Reviewed Journal)
Paz, Z., Garcia-Pedrajas, M.D., Andrews, D.L., Klosterman, S.J., Montanez, B., Gold, S.E. 2011. One step construction of agrobacterium recombination-ready plasmids (OSCAR), an efficient and robust tool for ATMT based gene deletion construction in fungi. Fungal Genetics and Biology. 48:677-684.
Real-time PCR for the quantification of fungi in planta - (Book / Chapter)
Klosterman, S.J. 2012. Real-time PCR for the quantification of fungi in planta. In: Bolton, M.D., Thomma, B.P.H.J., editors. Plant Fungal Pathogens: Methods and Protocols. Series: Methods in Molecular Biology. Volume 835. New York, NY: Humana Press. p. 121-131.
Iceberg lettuce breeding lines with resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae. - (Peer Reviewed Journal)
Hayes, R.J., Maruthachalam, K., Vallad, G.E., Klosterman, S.J., Simko, I., Luo, Y., Subbarao, K.V. 2011. Iceberg lettuce breeding lines with resistance to Verticillium wilt caused by race 1 isolates of Verticillium dahliae. HortScience. 46(3):501-504.
Identification of pathogenicity-related genes in the vascular wilt fungus verticillium dahliae by agrobacterium tumefaciens-mediated t-DNA insertional mutagenesis. - (Peer Reviewed Journal)
Maruthachalam, K., Klosterman, S.J., Kang, S., Hayes, R.J., Subbarao, K.V. 2011. Identification of pathogenicity-related genes in the vascular wilt fungus verticillium dahliae by agrobacterium tumefaciens-mediated t-DNA insertional mutagenesis. Molecular Biotechnology. PMID:21424547.
Verticillium comparative genomics yields insights into niche adaptation by plant vascular wilt pathogens Reprint Icon - (Abstract Only)
Klosterman, S.J., Subbarao, K.V., Kang, S., Veronese, P., Gold, S.E., Thomma, B.J., Chen, Z., Henrissat, B., Lee, Y., Park, J., Garcia-Pedrajas, M.D., Barbara, D., Anchieta, A.G., De Jonge, R., Santhanam, P., Maruthachalam, K., Atallah, Z.K., Amyotte, S., Paz, Z., Inderbitzin, P., Hayes, R.J., Heiman, D.I., Young, S., Zeng, Q., Engels, R., Galagan, J., Cuomo, C., Dobinson, K.F., Ma, L. 2011. Verticillium comparative genomics yields insights into niche adaptation by plant vascular wilt pathogens. Plant and Animal Genome Conference. Online.
Molecular variation among isolates of verticillium dahliae and PCR-based differentiation of races. - (Peer Reviewed Journal)
Maruthachalam, K., Atallah, Z.K., Vallad, G.E., Klosterman, S.J., Hayes, R.J., Davis, M.R., Subbarao, K. 2010. Molecular variation among isolates of verticillium dahliae and PCR-based differentiation of races. Phytopathology. 11:1222-30.
Molecular variation among isolates of verticillium dahliae and PCR-based differentiation of races. - (Peer Reviewed Journal)
Maruthachalam, K., Atallah, Z.K., Vallad, G.E., Klosterman, S.J., Hayes, R.J., Davis, R., Subbarao, K.V. 2010. Molecular variation among isolates of verticillium dahliae and PCR-based differentiation of races. Phytopathology. 100(11):1222-30.
A qPCR assay for detection and quantification of Verticillium dahliae in spinach seed. - (Abstract Only)
Rauscher, G.M., Mou, B., Hayes, R.J., Koike, S.T., Maruthachalam, K., Subbarao, K.V., Klosterman, S.J. 2010. A qPCR assay for detection and quantification of Verticillium dahliae in spinach seed.. Phytopathology. 100:S107.
Population analyses of the vascular plant pathogen Verticillium dahliae detect recombination and transcontinental gene flow - (Peer Reviewed Journal)
Atallah, Z.K., Maruthachalam, K.K., Du Toit, L.J., Koike, S.T., R. Michael, D., Klosterman, S.J., Hayes, R.J., Subbarao, K.V. 2010. Population analyses of the vascular plant pathogen, Verticillium dahliae detect recombination and transcontinental gene flow. Fungal Genetics and Biology. 47:416-422.
A soilless Verticillium wilt assay using an early flowering lettuce - (Peer Reviewed Journal)
Klosterman, S.J., Hayes, R.J. 2009. A soilless Verticillium wilt assay using an early flowering lettuce. Plant Disease. 93:691-698,2009.
Characterization of 22 highly polymorphic microsatellite loci in the cosmopolitan fungal plant pathogen Verticillium dahliae. Permanent Genetic Resources added to Molecular Ecology Resources - (Peer Reviewed Journal)
Atallah, Z., Maruthachalam, K., Davis, M.R., Klosterman, S.J., Subbarao, K. 2009. Characterization of 22 highly polymorphic microsatellite loci in the cosmopolitan fungal plant pathogen Verticillium dahliae. Permanent Genetic Resources added to Molecular Ecology Resources. Molecular Ecology Resources. Molecular Ecology Resources 9:1460-1466.
Identification of lettuce genes differentially expressed in a Verticillium dahliae-lettuce interaction by suppression subtractive hybridization - (Abstract Only)
Klosterman, S.J., Anchieta, A.G., Maruthachalam, K., Hayes, R.J., Subbarao, K.V. 2009. Identification of lettuce genes differentially expressed in a Verticillium dahliae-lettuce interaction by suppression subtractive hybridization. Phytopathology Supplement; APSnet (Plant Pathology Online).
Diversity, Pathogenicity And Control of Verticillium Species. - (Peer Reviewed Journal)
Klosterman, S.J., Atallah, Z.K., Vallad, G.E., Subbarao, K.N. 2009. Diversity, Pathogenicity And Control of Verticillium Species.. Annual Review of Phytopathology 2009, 47:39-62.
Verticillium comparative genomics--understanding pathogenicity and diversity. - (Abstract Only)
Klosterman, S.J., Veronese, P., Dobinson, K.F., Subbarao, K.V., Heiman, D.L., Young, S., Kodira, C.D., Alvarado, L., Galagan, J.E., Birren, B., Cuomo, C., Anchieta, A.G., Kang, S., Gold, S.E., Ma, L. 2008. Verticillium comparative genomics--understanding pathogenicity and diversity.. Phytopathology.
Development of rapid pathogenicity assay for Verticillium dahliae using early flowering lettuce. - (Abstract Only)
Klosterman, S.J., Hayes, R.J. Development of rapid pathogenicity assay for Verticillium dahliae using early flowering lettuce.. Phytopathology.
Population biology of Verticillium dahliae isolates from lettuce in the Sallinas Valley of Californis. - (Abstract Only)
Phytopathology 98:S14
Analysis of molecular variability among the isolated of Verticillium dahliae from diverse host species based on florescene-based amplified fragment length polymorphism. - (Abstract Only)
Phytopathology 98:S78
Ubc2, an Ortholog of the Yeast Ste50p Adaptor, Possesses a Basidiomycete-Specific Carboxy terminal Extension Essential for Pathogenicity Independent of Pheromone Response. - (Peer Reviewed Journal)
Klosterman, S.J., Martinez-Espinoza, A.D., Andrews, D.L., Seay, J.R., Gold, S.E. 2008. Ubc2, an Ortholog of the Yeast Ste50p Adaptor, Possesses a Basidiomycete-Specific Carboxy terminal Extension Essential for Pathogenicity Independent of Pheromone Response. Molecular Plant-Microbe Interactions. Vol. 21, No. 1 p. 110-121.
Comparative Verticillium genomics - (Abstract Only)
Klosterman, S.J., Subbarao, K.V., Dobinson, K.F., Veronese, P., Kang, S., Gold, S.E., Ma, L. 2007. Comparative Verticillium genomics. Fungal Genetics Newsletter. 54(Suppl) pg. 75.
Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis - (Peer Reviewed Journal)
Kamper, J., Kahmann, R., Bolker, M., Ma, L., Brefort, T., Saville, B.J., Banuett, F., Kronstad, J.W., Gold, S.E., Muller, O., Perlin, M.H., Wosten, H.A., Devries, R., Ruiz-Herrera, J., Reynaga-Pena, C.G., Snetselaar, K., Mccann, M., Perez-Martin, J., Feldrugge, M., Basse, C.W., Steinberg, G., Ibeas, J., Holloman, W., Guzman, P., Farman, M., Stajich, J.E., Sentandreu, R., Gonzalez-Prietro, J.M., Kennell, J.C., Molina, L., Schirawski, J., Mendoza-Mendoza, A., Greilinger, D., Munch, K., Rossel, N., Scherer, M., Vranes, M., Ladendorf, O., Vincon, V., Fuchs, U., Sandrock, B., Meng, S., Ho, E.C., Cahill, M.J., Boyce, K.J., Klose, J., Klosterman, S.J., Deelstra, H.J., Ortiz-Castellanos, L., Li, W., Sanchez-Alonso, P., Schreier, P.H., Hauser-Hahn, I., Vaupel, M., Koopmann, E., Griedrich, G., Voss, H., Schluter, T., Margolis, J., Platt, D., Swimmer, C., Gnirke, A., Chen, F., Vysotskaia, V., Mannhaupt, G., Guldener, U., Munsterkotter, M., Haase, D., Oesterheld, M., Mewes, H., Maucell, E.W., Decaprio, D., Wade, C.M., Butler, J., Young, S., Jaffe, D.D., Calvo, S., Nusbaum, C., Galagan, J., Birren, B.W. 2006. Insights from the genome of the biotrophic fungal plant pathogen Ustilago maydis. Nature. 2006. 444:97-101. DOI:10.1038/nature05248
Genetics of morphogenesis and pathogenic development of Ustilago maydis. - (Book / Chapter)
Klosterman, S.J., Perlin, M.H., Garcia-Pedrajas, M., Covert, S.F., Gold, S.E. 2007. Genetics of morphogenesis and pathogenic development of Ustilago maydis. Advances in Genetics. Ed. Dunlap, Jay C. Vol. 57. pg. 1-47.
Last Modified: 7/23/2014