Projects

PROCESS TECHNOLOGIES FOR PRODUCING BIOFUELS AND COPRODUCTS FROM LIGNOCELLULOSIC FEEDSTOCKS Appropriated (D)   Accession Number: 418680

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Reduction of fermentation lag phase in biofuel production using a novel activated biochar material - (Proceedings)

Klasson, K.T., Dien, B.S., Hector, R.E. 2012. Reduction of fermentation lag phase in biofuel production using a novel activated biochar material. In: Proceedings of the U.S.-Japan Cooperative Program in Natural Resources Food and Agriculture Panel, USDA-ARS, December 8-13, 2012, Wyndmoor, Pennsylvania. pp. 1-2.

Genetically engineered Escherichia coli FBR5: Part I. Comparison of high cell density bioreactors for enhanced ethanol production from xylose - (Peer Reviewed Journal)

Qureshi, N., Dien, B.S., Liu, S., Saha, B.C., Hector, R.E., Cotta, M.A., Hughes, S.R. 2012. Genetically engineered Escherichia coli FBR5: Part I. Comparison of high cell density bioreactors for enhanced ethanol production from xylose. Biotechnology Progress. 28(5):1167-1178.

Genetically engineered Escherichia coli FBR5: Part II. Ethanol production from xylose and simultaneous product recovery - (Peer Reviewed Journal)

Qureshi, N., Dien, B.S., Liu, S., Saha, B.C., Cotta, M.A., Hughes, S.R., Hector, R.E. 2012. Genetically engineered Escherichia coli FBR5: Part II. Ethanol production from xylose and simultaneous product recovery. Biotechnology Progress. 28(5):1179-1185.

Liquid chromatography-mass spectrometry investigation of enzyme-resistant xylooligosaccharide structures of switchgrass associated with ammonia pretreatment, enzymatic saccharification, and fermentation - (Peer Reviewed Journal)

Bowman, M.J., Dien, B.S., Hector, R.E., Sarath, G., Cotta, M.A. 2012. Liquid chromatography-mass spectrometry investigation of enzyme-resistant xylooligosaccharide structures of switchgrass associated with ammonia pretreatment, enzymatic saccharification, and fermentation. Bioresource Technology. 110:437-447.

Plant cell walls to ethanol. - (Peer Reviewed Journal)

Jordan, D.B., Bowman, M.J., Braker, J.D., Dien, B.S., Hector, R.E., Lee, C.C., Mertens, J.A., Wagschal, K.C. 2012. Plant cell walls to ethanol. Biochemical Journal. 442:247-252.

Subsite binding energies of an exo-polygalacturonase using isothermal titration calorimetry - (Peer Reviewed Journal)

Mertens, J.A., Hector, R.E., Bowman, M.J. 2012. Subsite binding energies of an exo-polygalacturonase using isothermal titration calorimetry. Thermochimica Acta. 527:219-222.

Biochemical processing of reed canarygrass into fuel ethanol - (Peer Reviewed Journal)

Dien, B.S., Casler, M.D., Hector, R.E., Iten, L.B., Nichols, N.N., Mertens, J.A., Cotta, M.A. 2012. Biochemical processing of reed canarygrass into fuel ethanol. International Journal of Low-Carbon Technologies. 7:338-347.

Effect of cellulosic sugar degradation products (furfural and hydroxymethylfurfural) on acetone-butanol-ethanol (ABE) fermentation using Clostridium beijerinckii P260 - (Peer Reviewed Journal)

Qureshi, N., Bowman, M.J., Saha, B.C., Hector, R.E., Berhow, M.A., Cotta, M.A. 2012. Effect of cellulosic sugar degradation products (furfural and hydroxymethylfurfural) on acetone-butanol-ethanol (ABE) fermentation using Clostridium beijerinckii P260. Journal of Food and Bioproducts Processing. 90:533-540.

Saccharomyces cerevisiae engineered for xylose metabolism requires gluconeogenesis and the oxidative branch of the pentose phosphate pathway for aerobic xylose assimilation - (Peer Reviewed Journal)

Hector, R.E., Mertens, J.A., Bowman, M.J., Nichols, N.N., Cotta, M.A., Hughes, S.R. 2011. Saccharomyces cerevisiae engineered for xylose metabolism requires gluconeogenesis and the oxidative branch of the pentose phosphate pathway for aerobic xylose assimilation. Yeast. 28:645-660.

Identifying enzyme resistant xylo-oligomers from processing switchgrass to bioethanol - (Abstract Only)

Bowman, M.J., Dien, B.S., Hector, R.E., Sarath, G., Cotta, M.A. 2011. Identifying enzyme resistant xylo-oligomers from processing switchgrass to bioethanol [abstract]. American Society for Mass Spectrometry. p. 66.

Dilute ammonium hydroxide pretreatment of reed canary grass and its simultaneous saccharification and fermentation to ethanol using a xylose-fermenting Saccharomyces cerevisiae strain - (Abstract Only)

Dien, B.S., Hector, R.E., Casler, M.D., Cotta, M.A. 2011. Dilute ammonium hydroxide pretreatment of reed canary grass and its simultaneous saccharification and fermentation to ethanol using a xylose-fermenting Saccharomyces cerevisiae strain [abstract]. In: Proceedings of the 33rd Symposium on Biotechnology for Fuels and Chemicals, May 2-5, 2011, Seattle, Washington. Paper No. 4-18.

Switchgrass alkaline pretreatment, enzymatic saccharification, and fermentation with residual oligosaccharide product analysis by mass spectrometry - (Abstract Only)

Bowman, M.J., Dien, B.S., Hector, R.E., Sarath, G., Cotta, M.A. 2011. Switchgrass alkaline pretreatment, enzymatic saccharification, and fermentation with residual oligosaccharide product analysis by mass spectrometry [abstract]. In: Proceedings of the 33rd Symposium on Biotechnology for Fuels and Chemicals, May 2-5, 2011, Seattle, Washington. Paper NO. 10-19.

Butanol productivity enhancers in wheat straw hydrolyzate: employing potential of enhanced reaction rate - (Abstract Only)

Qureshi, N., Bowman, M.J., Hector, R.E., Saha, B.C., Cotta, M.A. 2011. Butanol productivity enhancers in wheat straw hydrolyzate: employing potential of enhanced reaction rate [abstract]. In: Proceedings of the 33rd Symposium on Biotechnology for Fuels and Chemicals, May 2-5, 2011, Seattle, Washington. Paper No. 11-11.

Enhancing alfalfa conversion efficiencies for sugar recovery and ethanol production by altering lignin composition - (Peer Reviewed Journal)

Dien, B.S., Miller, D.J., Hector, R.E., Dixon, R.A., Chen, F., McCaslin, M., Risen, P., Sarath, G., Cotta, M.A. 2011. Enhancing alfalfa conversion efficiencies for sugar recovery and ethanol production by altering lignin composition. Bioresource Technology. 102(11):6479-6486.

Engineering industrial Saccharomyces cerevisiae strains for xylose fermentation and comparison for switchgrass conversion - (Peer Reviewed Journal)

Hector, R.E., Dien, B.S., Cotta, M.A., Qureshi, N. 2011. Engineering industrial Saccharomyces cerevisiae strains for xylose fermentation and comparison for switchgrass conversion. Journal of Industrial Microbiology and Biotechnology. 38(9):1193-1202.

Cloning and Expression of Novel Bacterial Xylose Isomerases in Saccharomyces cerevisiae - (Abstract Only)

Hector, R.E., Dien, B.S., Cotta, M.A., Hughes, S.R. 2010. Cloning and expression of novel bacterial xylose isomerases in Saccharomyces cerevisiae. Biotechnology for Fuels and Chemicals Symposium. Abstract #1.

Biochemical Conversion of Reduced Lignin Alfalfa Stems Into Ethanol - (Abstract Only) - (22-Apr-10)

Production of Butanol (a Biofuel) from Agricultural Residues: Part II - Use of Corn Stover and Switchgrass Hydrolysates - (Peer Reviewed Journal)

Qureshi, N., Saha, B.C., Hector, R.E., Dien, B., Hughes, S., Liu, S., Iten, L., Bowman, M.J., Sarath, G., Cotta, M.A. 2010. Production of butanol (a Biofuel) from agricultural residues: Part II - Use of corn stover and switchgrass hydrolysates. Biomass and Bioenergy. 34(4):566-571.

Production of Butanol (A Biofuel) from Agricultural Residues: Part I - Use of Barley Straw Hydrolysate - (Peer Reviewed Journal)

Qureshi, N., Saha, B.C., Dien, B., Hector, R.E., Cotta, M.A. 2010. Production of Butanol (a Biofuel) from Agricultural Residues: Part I - Use of Barley Straw Hydrolysate. Biomass and Bioenergy. 34(4):559-565.

Enhanced Production of Carboxylic Acids by Engineering of Rhizopus - (Abstract Only)

Skory, C.D., Hector, R.E., Gorsich, S.W., Rich, J.O. 2009. Enhanced Production of Carboxylic Acids by Engineering of Rhizopus [abstract].

Novel Lactate Transporters from Carboxylic Acid-Producing Rhizopus - (Abstract Only)

Skory, C.D., Hector, R.E., Gorsich, S.W., Rich, J.O. 2009. Novel lactate transporters from carboxylic acid-producing Rhizopus [abstract]. United States-Japan Natural Resources Food and Agriculture Panel. p. 160-163.

Novel Lactate Transporters from Carboxylic Acid-Producing Rhizopus - (Proceedings)

Skory, C.D., Hector, R.E., Gorsich, S.W., Rich, J.O. 2009. Novel lactate transporters from carboxylic acid-producing Rhizopus. United States-Japan Cooperative Program in Natural Resources. p. 160-163.

Report membrane transport of lactic acid in the filamentous fungus Rhizopus - (Peer Reviewed Journal)

Skory, C.D., Hector, R.E., Gorsich, S.W., Rich, J.O. 2009. Report membrane transport of lactic acid in the filamentous fungus Rhizopus. KKU Research Journal. 15(9):826-831.

Genetic Engineering of Rhizopus for Enhancing Lactic Acid Production - (Abstract Only)

Rich, J.O., Skory, C.D. 2009. Genetic Engineering of Rhizopus for enhancing lactic acid production [abstract]. Conference on Fermentation Technology for Value Added Agricultural Products. Paper Fer406. p. 25.

Analysis of a Functional Lactate Permease in the Fungus Rhizopus - (Peer Reviewed Journal)

Skory, C.D., Hector, R.E., Gorsich, S., Rich, J.O. 2010. Analysis of a functional lactate permease in the fungus Rhizopus. Enzyme and Microbial Technology. 46(1):43-50.

The Saccharomyces cerevisiae YMR315W Gene Encodes an NADP(H)-Specific Oxidoreductase Regulated by the Transcription Factor Stb5p in Response to NADPH Limitation - (Peer Reviewed Journal)

Hector, R.E., Bowman, M.J., Skory, C.D., Cotta, M.A. 2009. The Saccharomyces cerevisiae YMR315W gene encodes an NADP(H)-specific Oxidoreductase regulated by the transcription factor Stb5p in response to NADPH limitation. New Biotechnology. 26(3/4):171-180.

Evaluation of engineered xylose-fermenting industrial strains of Saccharomyces cerevisiae for improved ethanol production from lignocellulosic feedstocks - (Abstract Only) - (06-May-09)

Conversion of switchgrass to sugars and ethanol using dilute ammonium hydroxide pretreatment - (Abstract Only) - (06-May-09)

GMAX Yeast Background Strain Made from Industrial Tolerant Saccharomyces Cerevisiae Engineered to Convert Pretreated Lignocellulosic Starch and Cellulosic Sugars Universally to Ethanol Anaerobically - (Abstract Only)

Hughes, S.R., Tasaki, K., Moser, B., Doll, K., Butt, T., Sterner, D., Bischoff, K., Hector, R., Jones, M., and Bang, S. 2009. GMAX yeast background strain made from industrial tolerant Saccharomyces cerevisiae engineered to convert pretreated Lignocellulosic starch and cellulosic sugars universally to ethanol anaerobically [abstract]. Cambridge Healthtech Institute, Advanced Biofuels Development Summit. Abstract No. 2. p. 3.

Automated High Throughput GMAX-L Strains of Saccharomyces Cereviciae for Profitable Cellulosic Ethanol Production from Industrial Hydrosylates - (Abstract Only)

Hughes, S.R., Tasaki, K., Doll, K.M., Moser, B.R., Rich, J.O., Qureshi, N., Hector, R.E., Dien, B.S., Cotta, M.A., Bischoff, K.M., Liu, S., Bang, S., Jones, M. 2009. Automated high throughput GMAX-L strains of Saccharomyces cereviciae for profitable cellulosic ethanol production from industrial hydrosylates [abstract]. Society for In-Vitro Biology. p. 122.

Automated Yeast Transformation Protocol to Engineer S. cerevisiae Strains for Cellulosic Ethanol Production with Open Reading Frames that Express Proteins Binding to Xylose Isomerase Identified using Robotic Two-hybrid Screen - (Peer Reviewed Journal)

Hughes, S.R., Rich, J.O., Bischoff, K.M., Hector, R.E., Qureshi, N., Saha, B.C., Dien, B.S., Liu, S., Jackson Jr, J.S., Sterner, D.E., Butt, T.R., Labaer, J., Cotta, M.A. 2009. Automated yeast transformation protocol to engineer S. cerevisiae strains for cellulosic ethanol production with open reading frames that express proteins binding to xylose isomerase identified using robotic two-hybrid screen. Journal of the Association for Laboratory Automation. 8:200-212.

Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings - (Abstract Only)

Hughes, S.R., Doll, K., Moser, B., Bang, S., Rich, J., Qureshi, N., Hector, R., Dien, B., Cotta, M., Bischoff, K., Liu, S., Jones, M. 2009. Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings [abstract]. LabAutomation 2009. Abstract No. 109. p. 42.

Automated Yeast Mating Protocol Using Open Reading Frames from Saccharomyces cerevisiae Genome to Improve Yeast Strains for Cellulosic Ethanol Production - (Peer Reviewed Journal)

Hughes, S.R., Hector, R.E., Rich, J.O., Qureshi, N., Bischoff, K.M., Dien, B.S., Saha, B.C., Liu, S., Jackson Jr, J.S., Sterner, D.E., Butt, T.R., Labaer, J., Cotta, M.A. 2009. Automated yeast mating protocol using open reading frames from Saccharomyces cerevisiae genome to improve yeast strains for cellulosic ethanol production. Journal of the Association for Laboratory Automation. 8:190-199.

Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system - (Peer Reviewed Journal)

Hughes, S.R., Sterner, D.E., Bischoff, K.M., Hector, R.E., Dowd, P.F., Qureshi, N., Bang, S.S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Dien, B.S., Mertens, J.A., Caughey, R.J., Liu, S., Butt, T.R., Labaer, J., Cotta, M.A., Rich, J.O. 2009. Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system. Plasmid Journal. 61(1):22-38.

Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars - (Abstract Only)

Hughes, S.R., Javers, J., Labaer, J., Butt, T., Hector, R.E., Dien, B.S., Saha, B.C., Bischoff, K.M., Kohl, S., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars [abstract]. Society of Industrial Microbiology. Session 34, S164, p. 106.

Expression of a Heterologous Xylose Transporter in a Saccharomyces cerevisiae Strain Engineered to Utilize Xylose Improves Aerobic Xylose Co-consumption - (Peer Reviewed Journal)

Hector, R.E., Qureshi, N., Hughes, S.R., Cotta, M.A. 2008. Expression of a heterologous xylose transporter in a Saccharomyces cerevisiae strain engineered to utilize xylose improves aerobic xylose consumption. Applied Microbiology and Biotechnology. 80(4):675-684.

Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic sugars anaerobically to ethanol - (Abstract Only)

Hughes, S.R., Bischoff, K.M., Hector, R.E., Dien, B.S., Saha, B.C., Javers, J., Gibbons, W., Bang, S., Nelson, R., Standish, K., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae engineered to convert pretreated Lignocellulosic sugars anaerobically to ethanol [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit, Seminar 6. p. 1.

Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain - (Abstract Only)

Hughes, S.R., Jackson Jr, J.S., Dowd, P.F., Hector, R.E., Bischoff, K.M., Sterner, D., Bang, S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Li, X., Caughey, R.J., Liu, S., Skory, C.D., Butt, T., Labaer, J., Dien, B.S., Saha, B.C., Bowen, M., Qureshi, N., Rich, J.O., Leathers, T.D., Cotta, M.A., Farrelly, P., Slininger P.J. 2008. Three-vector system for high-level functional expression of value-added co-products with Xylose Isomerase and Xylulokinase in an industrial Saccharomyces cerevisiae strain [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit. Poster #1. p. 4.

Expression of a heterologous xylose transporter in a Saccharomyces cerevisiae strain engineered to utilize xylose increases xylose uptake and improves xylose/glucose co-consumption - (Abstract Only)

Hector, R.E., Qureshi, N., Hughes, S.R., Cotta, M.A. 2008. Expression of a heterologous xylose transporter in a Saccharomyces cerevisiae strain engineered to utilize xylose increases xylose uptake and improves xylose/glucose co-consumption [abstract]. In: Proceedings of the 30th Symposium on Biotechnology for Fuels and Chemicals, May 4-7, 2008, New Orleans, LA. p. 131.

The YMR315W gene from Saccharomyces cerevisiae codes for an alcohol dehydrogenase and is required for full resistance to oxidative stress - (Abstract Only) - (07-May-08)

Removal of Fermentation Inhibitors from Alkaline Peroxide Pretreated and Enzymatically Hydrolyzed Wheat Straw: Production of Butanol from Hydrolysate Using Clostridium beijerinckii in Batch Reactors - (Peer Reviewed Journal)

Qureshi, N., Saha, B.C., Hector, R.E., Cotta, M.A. 2008. Removal of Fermentation Inhibitors from Alkaline Peroxide Pretreated and Enzymatically Hydrolyzed Wheat Straw: Production of Butanol from Hydrolysate Using Clostridium beijerinckii in Batch Reactors. Biomass and Bioenergy. 32(12):1353-1358.

Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain - (Peer Reviewed Journal)

Hughes, S.R., Dowd, P.F., Hector, R.E., Panavas, T., Sterner, D.E., Qureshi, N., Bischoff, K.M., Bang, S.B., Mertens, J.A., Johnson, E.T., Li, X., Jackson Jr, J.S., Caughey, R.J., Riedmuller, S.B., Bartolett, S., Liu, S., Rich, J.O., Farrelly, P.J., Butt, T.R., Labaer, J., Cotta, M.A. 2008. Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain. Journal of Peptide Science. 14(9):1039-1050. Available: http://www3.interscience.wiley.com/cgi-bin/fulltext/119030240/PDFSTART.

Regulatory concerns associated with use of value-added recombinant proteins and peptides screened in hgh-throughput for expression in fuel ethanol yeast strains - (Abstract Only)

Hughes, S.R., Dowd, P.F., Hector, R.E., Bischoff, K.M., Rich, J.O., Cotta, M.A., Slininger, P.J., Rosentrater, K.A. 2008. Regulatory concerns associated with use of value-added recombinant proteins and peptides screened in hgh-throughput for expression in fuel ethanol yeast strains [abstract]. LabAutomation 2008, Track 5, Seminar 4. p. 5.

ENGINEERING SACCHAROMYCES CEREVISIAE FOR ETHANOL PRODUCTION FROM AGRICULTURAL WASTE PRODUCTS - (Abstract Only)

Hector, R.E., Qureshi, N., Hughes, S.R., Cotta, M.A. 2007. Engineering Saccharomyces cerevisiae for ethanol production from agricultural waste products [abstract]. Yeast Cell Biology. Paper No. 176.

BUTANOL PRODUCTION FROM WHEAT STRAW BY SIMULTANEOUS SACCHARIFICATION AND FERMENTATION USING CLOSTRIDIUM BEIJERINCKII: PART I-BATCH FERMENTATION - (Peer Reviewed Journal)

Qureshi, N., Saha, B.C., Hector, R.E., Hughes, S.R., Cotta, M.A. 2008. Butanol production from wheat straw by simultaneous saccharification and fermentation using Clostridium beijerinckii: Part I - batch fermentation. Biomass and Bioenergy. 32:168-175.

High-Throughput Fully Automated Construction of a Multiplex Library of Mutagenized Open Reading Frames for an Insecticidal Peptide Using a Plasmid-Based Functional Proteomic Robotic Workcell with Improved Vacuum System - (Peer Reviewed Journal)

Hughes, S.R., Dowd, P.F., Hector, R.E., Riedmuller, S.B., Bartolett, S., Mertens, J.A., Qureshi, N., Liu, S., Bischoff, K.M., Li, X., Jackson Jr, J.S., Sterner, D., Panavas, T., Cotta, M.A., Farrelly, P.J., Butt, T. 2007. High-throughput fully automated construction of a multiplex library of mutagenized open reading frames for an insecticidal peptide using a plasmid-based functional proteomic robotic workcell with improved vacuum system. Journal of Laboratory Automation. 12(4):202-212.

Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose Expressing "Stealth" Insecticidal Peptides. - (Abstract Only)

Hughes, S.R., Li, X., Hector, R.E., Bischoff, K.M., Cotta, M.A., Mertens, J.A., Dowd, P.F., Johnson, E.T. 2007. Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and characterization of yeast strains optimized for growth on xylose expressing "stealth" insecticidal peptides [abstract]. American Chemical Society. p. 108.

Adaptation to an automated platform of algorithmic combinations of advantageous mutations in genes generated using amino acid scanning mutational strategy. - (Abstract Only)

Riedmuller, S., Hughes, S.R., Hector, R.E., Bischoff, K.M., Farrelly, P., Cotta, M.A., Li, X. 2007. Adaptation to an automated platform of algorithmic combinations of advantageous mutations in genes generated using amino acid scanning mutational strategy [abstract]. PEPTALK 2007. Cambridge Healthtech Institute. Seminar 43. p. 4.

Fully automated molecular biology: Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Optimized "Trojan Horse" Amino Acid Scanning Mutational Inserts. - (Abstract Only)

Hughes, S.R., Qureshi, N., Mertens, J.A., Cotta, M.A., Hector, R.E., Bischoff, K.M., Liu, S., Li, X-L., 2007. Fully automated molecular biology: Plasmid-based functional proteomic workcell evaluation and characterization of yeast strains with optimized "Trojan Horse" amino acid scanning mutational inserts [abstract]. PEPTALK 2007. Cambridge Healthtech Institute. Poster 509, p. 4.