Projects

Technologies for Detecting and Determining the Bioavailability of Bacterial Toxins Appropriated (D)   Accession Number: 421099

DETECTION OF BOTULINUM TOXIN IN ANIMAL SERA AND ORGANS Reimbursable (R)   Accession Number: 418580

DEVELOPMENT OF DETECTION TECHNOLOGIES FOR BACTERIAL NEUROTOXINS AND THEIR VALIDATION IN FOOD MATRICES Specific Cooperative Agreement (S)   Accession Number: 422443

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Translocation of botulinum neurotoxins and associated proteins across intestinal epithelial cells(Abstract) - (Abstract Only) - (16-Mar-13)

Detection of botulinum neurotoxin serotypes A and B using a chemiluminescent versus electrochemiluminescent immunoassay in food and serum - (Peer Reviewed Journal)

Cheng, L.W., Stanker, L.H. 2013. Detection of botulinum neurotoxin serotypes A and B using a chemiluminescent versus electrochemiluminescent immunoassay in food and serum. Journal of Agricultural and Food Chemistry. 61(3):755-760 doi:10.1021/jf3041963.

Development and characterization of six monoclonal antibodies to hemagglutinin-70 (HA70) of Clostridium botulinum and their application in a sandwich ELISA - (Peer Reviewed Journal)

Scotcher, M.C., Cheng, L.W., Ching, K.H., Mcgarvey, J.A., Hnasko, R.M., Stanker, L.H. 2013. Development and characterization of six monoclonal antibodies to hemagglutinin-70 (HA70) of Clostridium botulinum and their application in a sandwich ELISA. Monoclonal Antibodies in Immunodiagnosis and Immunotherapy. 32(1):6-15. doi: 10.1089/mab.2012.0071.

Monoclonal antibodies and reagents for botulinum research - (Proceedings)

Stanker, L.H., Cheng, L.W. 2012. Monoclonal antibodies and reagents for botulinum research. The Botulinum Journal. 2(2):150-155 doi: 10.1504/tbj2012.050197.

Detection of botulinum neurotoxin serotypes A and B using chemiluminescence and electrochemiluninescene immunoassays in food and serum matrices - (Abstract Only) - (21-Dec-11)

Current methods for detecting the presence of botulinum neurotoxins in food and other biological samples - (Book / Chapter)

Cheng, L.W., Land, K.M., Stanker, L.H. 2012. Current methods for detecting the presence of botulinum neurotoxins in food and other biological samples. Morse, S., editor. Bioterrorism. Rijeka, Croatia: Intech. p. 1-16.

Comparison of oral toxicological properties of botulinum neurotoxin - (Peer Reviewed Journal)

Cheng, L.W., Henderson II, T.D. 2011. Comparison of oral toxicological properties of botulinum neurotoxin. Toxicon. 58:62-67.

Botulinum neurotoxin: a deadly protease with applications to human medicine - (Book / Chapter)

Land, K.M., Cheng, L.W. 2010. Botulinum neurotoxin: a deadly protease with applications to human medicine. Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology. Spain. Formatex Research Center. 965-971.

Ricin toxicokinetics and its sensitive detection in mouse sera or feces using immuno-PCR - (Peer Reviewed Journal)

He, X., Mcmahon, S.A., Henderson II, T.D., Griffey, S.M., Cheng, L.W. 2010. Ricin toxicokinetics and its sensitive detection in mouse sera or feces using immuno-PCR. PLoS One. 5(9):e12858. doi:10.1371/journal.pone.0012858.

Detection of Botulinum Neurotoxin Serotype B at Sub Mouse LD50 Levels by a Sandwich Immunoassay and its Application to Toxin Detection in Milk - (Peer Reviewed Journal)

Scotcher, M.C., Cheng, L.W., Stanker, L.H. 2010. Detection of Botulinum Neurotoxin Serotype B at Sub Mouse LD50 Levels by a Sandwich Immunoassay and its Application to Toxin Detection in Milk. PLos One. 5(6):e11047.doi:10.1371/journal.pone.0011047.

Comparison of Toxicological Properties of Botulinum Neurotoxin Serotypes A and B in Mice - (Abstract Only)

Cheng, L.W., Henderson Ii, T.D., Scotcher, M.C., Stanker, L.H. 2009. Comparison of Toxicological Properties of Botulinum Neurotoxin Serotypes A and B in Mice. American Chemical Society [Abstract]. Paper No. 26541

Antibody Protection Against Botulinum Neurotoxin Intoxication In Mice - (Peer Reviewed Journal)

Cheng, L.W., Stanker, L.H., Henderson Ii, T.D., Lou, J., Marks, J.D. 2009. Antibody Protection Against Botulinum Neurotoxin Intoxication In Mice. Infection and Immunity. 77(10):4305-4313.

Detection of Protein Toxins - (Abstract Only)

Brandon, D.L., Cheng, L.W., He, X., Rasooly, R., Scotcher, M.C., Stanker, L.H., Carter, J.M. 2009. Detection of Protein Toxins. Meeting Abstract. Poster No. 8.

Botulinum Neurotoxin Research at the Western Regional Research Center - (Abstract Only) - (09-Apr-09)

Influence of Gene Expression on Variable Aflatoxin Production by Different Strains of Aspergillus flavus - (Abstract Only) - (01-Feb-09)

BOTOX and our tax dollars at work - (Other) - (06-Oct-08)

Immunochemical technologies for replacement of rodent bioassays in sensitive detection of toxins in foods - (Proceedings)

Carter, J.M., Cheng, L.W., He, X., Rasooly, R., Brandon, D.L., Stanker, L.H. 2010. Immunochemical technologies for replacement of rodent bioassays in sensitive detection of toxins in foods. Proceedings from Biodetection Technologies 6th Edition. 5:75-95.

Effect of food matrices on the biological activity of ricin - (Peer Reviewed Journal)

He, X., Lu, S.S., Cheng, L.W., Rasooly, R., Carter, J.M. 2008. Effect of food matrices on the biological activity of ricin. Journal of Food Protection 71(10):2053-2058.

Detection of botulinum neurotoxin-A activity in food by peptide cleavage assay - (Peer Reviewed Journal)

Rasooly, R., Stanker, L.H., Carter, J.M., Do, P.M., Cheng, L.W., He, X., Brandon, D.L. 2008. In vitro peptide cleavage assay for detection of Botulinum Neurotoxin-A activity in food. International Journal of Food Microbiiology. 126 (2008):135-139

Effects of purification on the bioavailability of botulinum neurotoxin type A - (Peer Reviewed Journal)

Cheng, L.W., Onisko, B.C., Johnson, E.A., Reader, J.R., Griffey, S.M., Larson, A.E., Tepp, W.H., Stanker, L.H., Brandon, D.L., Carter, J.M. 2008. Effects of purification on the bioavailability of botulinum neurotoxin type A. Toxicology. 249(2008):123-129.

Development and Partial Characterization of High-affinity Monoclonal Antibodies for Botulinum Toxin Type A and their use in Analysis of Milk by Sandwich ELISA - (Peer Reviewed Journal)

Stanker, L.H., Merrill, P.A., Scotcher, M.C., Cheng, L.W. 2008. Development and Partial Characterization of High-affinity Monoclonal Antibodies for Botulinum Toxin Type A and their use in Analysis of Milk by Sandwich ELISA. Journal of Immunological Methods, 336:1-8.

Application of a real time PCR method to detect castor toxin contamination in fluid milk and eggs - (Peer Reviewed Journal)

He, X., Carter, J.M., Brandon, D.L., Cheng, L.W., Mckeon, T.A. 2007. Application of a real time PCR method to detect castor toxin contamination in fluid milk and eggs. Journal of Agricultural and Food Chemistry. 55:6897-6902

Molecular Approaches for the Identification of Toxin in Foods - (Abstract Only) - (23-Feb-07)

BIOAVAILABILITY OF CRUDE RICIN IN FOOD MATRICES - (Abstract Only)

Cheng, L.W., Brandon, D.L., He, X., Chen, G.Q., Carter, J.M. 2006. Bioavailability of crude ricin in food matrices. [Abstract]. ACS National Meeting and Exposition. Poster AGFD 64

DEVELOPMENT OF MONOCLONAL ANTIBODIES SPECIFIC FOR RICIN - (Abstract Only)

Brandon, D.L., Cheng, L.W., He, X., Carter, J.M. 2006. Development of monoclonal antibodies specific for ricin. [Abstract]. ACS National Meeting and Exposition. Oral Presentation AGRO 234

DEVELOPMENT OF NEW MONOCLONAL ANTIBODIES SPECIFIC FOR BOTULINUM NEUROTOXIN TYPE A - (Abstract Only)

Stanker, L.H., Merrill, P.A., Cheng, L.W., Carter, J.M., Brandon, D.L. 2006. Development of new monoclonal antibodies specific for botulinum neurotoxin type a. [Abstract]. ACS National Meeting. Oral Presentation AGRO 235.