Projects

Virus-Vector-Host Interactions of Arboviral Diseases of Livestock Appropriated (D)   Accession Number: 415118

Integrated Approaches for Protection of Animals from Vector-Borne Pathogens Appropriated (D)   Accession Number: 418490

Countermeasures to Control and Eradicate Rift Valley Fever (Rfv) Appropriated (D)   Accession Number: 411726

Vector Competence of North American Mosquitoes for Rift Valley Fever Virus Nonfunded Cooperative Agreement (N)   Accession Number: 411446

Rift Valley Fever Young Animal Vaccine Model Specific Cooperative Agreement (S)   Accession Number: 417970

Expansion of the Kenyan Rift Valley Fever (Rvf) Veterinary Surveillance Program to Include Wildlife Species Specific Cooperative Agreement (S)   Accession Number: 412497

Evaluation of Rift Valley Fever (Rvf) Veterinary Diagnostic Reagents Specific Cooperative Agreement (S)   Accession Number: 412498

Development of Diagnostic for Rift Valley Fever Compatible with a Diva Vaccine Control Approach Specific Cooperative Agreement (S)   Accession Number: 414349

Research on Arthropod-Borne Diseases of Livestock Specific Cooperative Agreement (S)   Accession Number: 415136

Immunological Control of Rift Valley Fever Virus (Rvfv) in African Ruminants Specific Cooperative Agreement (S)   Accession Number: 418470

Research on Arthropod-Borne Diseases of Livestock and Wildlife Specific Cooperative Agreement (S)   Accession Number: 413170

Evaluation of Operator Safe Diagnostic Reagents for Rift Valley Fever Virus Specific Cooperative Agreement (S)   Accession Number: 410045

Publications

A Multiplex Real-time Reverse Transcription Polymerase Chain Reaction Assay for Detection and Differentiation of Bluetongue Virus and Epizootic Hemorrhagic Disease Virus Serogroups - (Peer Reviewed Journal)

Wilson, W.C., Hindson, B., O Hearn, E.S., Hall, S., Tellgren-Roth, C., Torres, C., Naraghi-Arani, P., Mecham, J.O., Lenhoff, R. 2009. A Multiplex Real-time Reverse Transcription Polymerase Chain Reaction Assay for Detection and Differentiation of Bluetongue Virus and Epizootic Hemorrhagic Disease Virus Serogroups. Journal of Veterinary Diagnostic Investigation, Vol 21:6, 760-770.

Development of Enzyme-Linked Immunosorbent Assays Using Expressed Proteins of Rift Valley Fever Virus - (Abstract)

Mecham, J.O., Miller, M.M. 2009. Development of Enzyme-Linked Immunosorbent Assays Using Expressed Proteins of Rift Valley Fever Virus. Meeting Abstract. Pg 294:P19-5

Characterization of Neutralization Determinants on Epizootic Hemorrhagic Disease Virus - (Abstract)

Mecham, J.O., Miller, M.M., Bennett, K.E., Reeves, W.K., Drolet, B.S., Wilson, W.C. 2009. Characterization of neutralization determinants on epizootic hemorrhagic disease virus. Meeting Abstract.

In Situ Immune Infrared Fluorescent Staining for Detection and Quantification of Bluetongue Virus in Cullicoides Insect Cell Culture - (Peer Reviewed Journal)

Mecham, J.O., Brown, P.L., Mcholland, L.E. 2009. In Situ Immune Infrared Fluorescent Staining for Detection and Quantification of Bluetongue Virus in Cullicoides Insect Cell Culture. Journal of Virological Methods. 158, 110-113.

USDA, ARS, ABDRL Research on Countermeasures for Rift Valley Fever Virus - (Abstract)

Wilson, W.C., Bennett, K.E., Drolet, B.S., Mecham, J.O., Miller, M.M., Reeves, W.K. 2008. USDA, ARS, ABDRL Research on Countermeasures for Rift Valley Fever Virus. Meeting Abstract.

Readiness and Capacity of the U.S. for the Introduction of Exotic Arthropod-Borne Viruses - (Proceedings/Symposium)

Wilson, W.C., Bennett, K.E., Mecham, J.O., Miller, M.M., Reeves, W.K., Drolet, B.S. 2008. Readiness and Capacity of the U.S. for the Introduction of Exotic Arthropod-Borne Viruses. U.S. Animal Health Association Conference, Greensboro, NC, October 24-29, 2008. PP. 220-221.

Report of the Bluetongue and Bovine Retroviruses Committee. - (Proceedings/Symposium)

Wilson, W.C., Drolet, B.S., Mecham, J.O., Miller, M.M., Reeves, W.K. 2009. Report of the Bluetongue and Bovine Retroviruses Committee.. Meeting Abstract.

An outbreak of Bluetongue Virus serotype 17 in sheep ranches of Wyoming. - (Abstract)

Miller, M.M., Brown, J., Mecham, J.O., Reeves, W.K. 2008. An outbreak of Bluetongue Virus serotype 17 in sheep ranches of Wyoming.. Meeting Abstract.

Report of the Bluetongue and Bovine Retroviruses Committee - (Proceedings/Symposium)

Wilson, W.C., Drolet, B.S., Kato, C.Y., Harpster, M.H., Bennett, K.E., O Hearn, E.S., Reeves, W.K., Mecham, J.O., Miller, M.M. 2008. Report of the Bluetongue and Bovine Retroviruses Committee. United States Animal Health Association Proceedings,Reno, NV, October 18-24, 2007, 111:207-208.

New Diagnostic Tools for Detecting Rift Valley Fever (RVF) and other Arboviruses - (Proceedings/Symposium)

Wilson, W.C., Drolet, B.S., Kato, C.Y., Harpster, M.H., Bennett, K.E., O Hearn, E.S., Reeves, W.K., Mecham, J.O., Miller, M.M., Stalknecht, D. 2008. New Diagnostic Tools for Detecting Rift Valley Fever (RVF) and other Arboviruses. United States Animal Health Association Proceedings, Reno, NV, October 18-24, 2007, 111:798-799.

Nucleic Acid Diagnostic Tools for Early Detection of Arthropod-Borne Animal Viruses - (Abstract)

Wilson, W.C., Lenhoff, R., Hindson, B., Torres, C., Stalknecht, D., Mead, D., O Hearn, E.S., Drolet, B.S., Mecham, J.O. 2007. Nucleic Acid Diagnostic Tools for Early Detection of Arthropod-Borne Animal Viruses. American Association of Veterinary Laboratory Diagnosticians.

Bluetongue virus serotype 17 sequence variation associated with neutralization - (Peer Reviewed Journal)

Wilson, W.C., Bernard, K., Isreal, B.A., Mecham, J.O. 2008. Bluetongue virus serotype 17 sequence variation associated with neutralization. Virus Research. DNA Sequence 2008 Jun;19(3):237-240

In situ immune infrared fluorescent staining for detection and quantification of bluetongue virus in insect cell culture - (Abstract)

Mecham, J.O., Brown, P.L. 2007. In situ immune infrared fluorescent staining for detection and quantification of bluetongue virus in insect cell culture. American Society for Virology Meeting. Corvallis, OR. July 14-18, 2007.

Glycosaminoglycan receptors facilitate infection of mammalian cells - (Abstract)

Mcholland, L.E., Mecham, J.O. 2007. Glycosaminoglycan receptors facilitate infection of mammalian cells. American Society for Virology Meeting. Corvallis, OR. July 14-18, 2007.

Phylogeny of bluetongue virus isolates by sequence analysis of the VP5 coding gene - (Abstract)

Wu, S., Letchworth, G., Mecham, J.O. 2007. Phylogeny of bluetongue virus isolates by sequence analysis of the VP5 coding gene. Tenth Annual Conference on Vaccine Research. Baltimore, MD. April 30-May 2, 2007.

"Detection of bluetongue virus in Culicoides cell cultures" - (Proceedings/Symposium) - (22-Sep-06)

An Update on the Arthropod-Borne Animal Diseases Research Laboratory, Laramie, Wyoming. - (Proceedings/Symposium) - (22-Sep-06)

Potential for Bluetongue Virus persistence in insect cells - (Abstract)

Wilson, W.C., Drolet, B.S., Bennett, K.E., O Hearn, E.S., Brown, P.L., Stuart, M.A., Mecham, J.O. 2006. Potential for Bluetongue Virus persistence in insect cells. American Society for Virology Meeting. Madison, WI. W22-9. 2006. Abstract.

PHYLOGENY OF BLUETONGUE VIRUS ISOLATES BY SEQUENCE ANALYSIS OF THE VP5 CODING GENE - (Abstract)

Mecham, J.O. 2006. Phylogeny of bluetongue virus isolates by sequence analysis of the vp5 coding gene. American Society for Virology Meeting. Paper No. P8-7

Potential for bluetongue virus persistence in insect cells. - (Abstract)

Wilson, W.C., Drolet, B.S., Bennett, K.E., O Hearn, E.S., Brown, P.L., Stuart, M.A., Mecham, J.O. 2006. Potential for bluetongue virus persistence in insect cells.. American Society for Virology Meeting. 25th Annual Meeting, W22-9.

DETECTION AND TITRATION OF BLUETONGUE VIRUS IN CULICOIDES INSECT CELL CULTURE BY AN ANTIGEN-CAPTURE ENZYME-LINKED IMMUNOSORBENT ASSAY - (Peer Reviewed Journal)

Mecham, J.O. 2006. Detection and titration of bluetongue virus in culicoides insect cell culture by an antigen-capture enzyme-linked immunosorbent assay. Journal of Virological Methods. 135:269-271

SEQUENCE ANALYSIS OF BTV-1 ISOLATED FROM A DEER IN LOUISIANA - (Abstract)

Mecham, J.O., Johnson, D.J., Wilson, W.C. 2006. Sequence analysis of btv-1 isolated from a deer in louisiana. United States Animal Health Association Proceedings.

Bluetongue virus mammalian cell surface receptors: Role of glycosaminologycans - (Peer Reviewed Journal)

Mcholland, L.E., Mecham, J.O. 2005. Bluetongue virus mammalian cell surface receptors: Role of glycosaminologycans. Journal of General Virology.

UPDATE ON BLUETONGUE VIRUS PERSISTENCE IN CULICOIDES SONORENSIS - (Abstract)

Wilson, W.C., Drolet, B.S., Mecham, J.O. 2005. Update on bluetongue virus persistence in Culicoides sonorensis. United States Animal Health Association Proceedings, 109th Annual Meeting.

Current status of BTV in the Americas - (Book/Chapter) - (22-Apr-05)

PERSISTENCE OF BLUETONGUE VIRUS SEROTYPE 2 (BTV-2) IN THE SOUTHEAST UNITED STATES - (Peer Reviewed Journal)

Mecham, J.O., Johnson, D.J. 2005. Persistence of bluetongue virus serotype 2 (BTV-2) in the southeast United States. Virus Research. 113:116-122

EPITOPE CODING SEQUENCE USED TO PREDICT SENSITIVITY OF BLUETONGUE VIRUS DIAGNOSTICS - (Abstract)

Mecham, J.O. 2005. Epitope coding sequence used to predict sensitivity of bluetongue virus diagnostics. American Society for Virology. Paper No. P33-1.

LACK OF DETECTABLE BLUETONGUE VIRUS IN SKIN OF SEROPOSITIVE CATTLE: IMPLICATIONS FOR VERTEBRATE OVERWINTERING OF BLUETONGUE VIRUS - (Peer Reviewed Journal)

White, D.M., Mecham, J.O. 2005. Lack of detectable bluetongue virus in skin of seropositive cattle: implications for vertebrate overwintering of bluetongue virus. Veterinaria Italiana.40:513-519.

PERSISTENCE OF BLUETONGUE VIRUS IN THE INSECT VECTOR AND ITS IMPLICATIONS FOR DISEASE CONTROL - (Proceedings/Symposium)

Mecham, J.O., White, D.M., Drolet, B.S., Wilson, W.C. 2005. Persistence of bluetongue virus in the insect vector and its implications for disease control. United States Animal Health Association Proceedings. 108:100-107.

DETECTION AND QUANTITATION OF BLUETONGUE VIRUS IN INSECT CELL CULTURE. - (Abstract)

Mecham, J.O. 2004. Detection and quantitation of bluetongue virus in insect cell culture [abstract]. American Society for Virology Meeting, p35-7, p. 270.

NEUTRALIZATION OF BLUETONGUE VIRUS IN VERTEBRATE AND INVERTEBRATE CELLS - (Abstract)

Mecham, J.O. 2003. Neutralization of bluetongue virus in vertebrate and invertebrate cells. 2003 ARS Immunology Research Workshop.

ANTIGEN CAPTURE COMPETITIVE ENZYME-LINKED IMMUNOSORBENT ASSAYS USING BACULOVIRUS-EXPRESSED ANTIGENS FOR DIAGNOSIS OF BLUETONGUE VIRUS AND EPIZOOTIC HEMORRHAGIC DISEASE VIRUS - (Peer Reviewed Journal)

Mecham, J.O., Wilson, W.C. 2004. Antigen capture competitive enzyme-linked immunosorbent assays using baculovirus-expressed antigens for diagnosis of bluetongue virus and epizootic hemorrhagic disease virus. Journal of Clinical Microbiology. 42(2):518-523.

FIELD-DEPLOYABLE REAL-TIME PCR DETECTION OF BLUETONGUE AND EPIZOOTIC HEMORRHAGIC DISEASE VIRAL RNA. - (Proceedings/Symposium)

Wilson, W.C., Stallknecht, D.E., Mecham, J.O. 2003. Field-deployable real-time PCR detection of bluetongue and epizootic hemorrhagic disease viral RNA [abstract]. OIE Symposium on Bluetongue Virus. Taormina, Italy. Paper No. D14, P. 117.

FIELD-DEPLOYABLE REAL-TIME PCR DETECTION OF BLUETONGUE AND EPIZOOTIC HEMORRHAGIC DISEASE VIRAL RNA. - (Peer Reviewed Journal)

Wilson, W.C., Stallknecht, D.E., Mecham, J.O., 2005. Field-deployable real-time pcr detection of bluetongue and epizootic hemorrhagic disease viral rna.. Veterinaria Italiana. 40(4), 587-593.

EVIDENCE THAT BLUETONGUE VIRUS SEROTYPE 2 (BTV-2) HAS PERSISTED IN THE SOUTHEASTERN UNITED STATES FOR THE PAST 20 YEARS - (Abstract)

Mecham, J.O. 2003. Evidence that bluetongue virus serotype 2 (btv-2) has persisted in the southeastern united states for the past 20 years. OIE Symposium on Bluetongue virus. Taormina, Italy. Paper No. C9, p. 96.

POSSIBILITY OF BLUETONGUE VIRUS IN BOVINE SKIN - (Abstract)

White, D.M., Mecham, J.O. 2003. Possibility of bluetongue virus in bovine skin [abstract]. OIE Symposium on Bluetongue virus. Taormina, Italy. Paper No. C9, p. 96.

REPORT OF THE COMMITTEE ON BLUETONGUE AND BOVINE RETROVIRUSES - (Proceedings/Symposium)

Mecham, J.O., Pearson, J.E., Ostlund, E., Mead, D., Jamieson, B., Dehaven, R., Faizi, N., Mayer, R.T. 2003. Report of the committee on bluetongue and bovine retroviruses. United States Animal Health Association Proceedings, Oct 9-16, 2003, San Diego, CA, 107:204-213.

EVIDENCE FOR REASSORTMENT IN THE NATURAL HISTORY OF BLUETONGUE VIRUS SEROTYPE 2. - (Abstract)

Mecham, J.O. 2003. Evidence for reassortment in the natural history of bluetongue virus serotype 2. American Society for Virology Meeting.

THE S7 GENE AND VP7 PROTEIN ARE HIGHLY CONSERVED AMONG TEMPORALLY AND GEOGRAPHICALLY DISTINCT AMERICAN ISOLATES OF EPIZOOTIC HEMORRHAGIC DISEASE VIRUS. - (Peer Reviewed Journal)

Mecham, J.O., Stallknecht, D., Wilson, W.C. 2003. The s7 gene and vp7 protein are highly conserved among temporally and geographically distinct american isolates of epizootic hemorrhagic disease virus. Virus Research 94:129-133.

CHARACTERIZATION OF CELL LINES DEVELOPED FROM FIELD POPULATIONS OF CULICOIDES SONORENSIS (DIPTERA: CERATOPOGONIDAE) - (Peer Reviewed Journal)

Mcholland, L. E., Mecham, J. O. 2003. Characterization of cell lines developed from field populations of Culicoides sonorensis (Diptera: Ceratopogonidae). Journal of Medical Entomology. 40 (3):348-351.

PROTECT YOURSELF AND YOUR SAMPLE: PROCESSING ARBOVIRUS INFECTED BITING MIDGES FOR VIRAL DETECTION ASSAYS AND DIFFERENTIAL EXPRESSION STUDIES - (Book/Chapter)

Drolet, B.S., Campbell, C.L., Mecham, J.O. 2003. Protect yourself and your sample: processing arbovirus infected biting midges for viral detection assays and differential expression studies. Anthology of Biosafety, VI: Arththropod-Borne Diseases.

REPORT OF THE COMMITTEE ON BLUETONGUE AND BOVINE RETROVIRUS - (Proceedings/Symposium)

Mecham, J.O., Ostlund, E., Gertonson, A., Stallknect, D., Kahn, S., Delver, L., Lopez, J., Kench, L., Giovannini, A., Murphy, M. 2002. Report of the committee on bluetongue and bovine retrovirus. United States Animal Health Association Proceedings, Oct 17-24, St. Louis, MO, p.150-162.

MOLECULAR EVOLUTION OF ORBIVIRUSES - (Proceedings/Symposium)

Wilson, W.C., Mecham, J.O. 2000. Molecular evolution of orbiviruses. United States Animal Health Association Proceedings. 104:169-180.

Molecular evolution of orbiviruses - (Proceedings/Symposium)

Wilson, W.C., Mecham, J.O. 2000. Molecular evolution of orbiviruses. Proc. U.S. Animal Health Assoc. 104:169-180. 2000.

DEVELOPMENT OF AN ENZYME-LINKED IMMUNOSORBENT ASSAY FOR THE DETECTION OF ANTIBODY TO EPIZOOTIC HEMORRHAGIC DISEASE OF DEER VIRUS - (Peer Reviewed Journal)

Mecham, J. O., Jochim, M. M. 2000. Development of an enzyme-linked immunosorbent assay for the detection of antibody to epizootic hemorrhagic disease of deer virus. Journal of Veterinary Diagnostic Investigation. 12:142-145.

EPIZOOTIC HEMORRHAGIC DISEASE: ANALYSIS OF TISSUES BY AMPLIFICATION AND IN SITU HYBRIDIZATION REVEALS WIDESPREAD ORBIVIRUS INFECTION AT LOW COPY NUMBERS - (Peer Reviewed Journal)

Brodie, S.J., Mecham, J.O., Norelius, S.E., Diem, K., Wilson, W.C. 1998. Epizootic hemorrhagic disease: analysis of tissues by amplification and in situ hybridization reveals widespread orbivirus infection at low copy numbers. Journal of Virology. 72:3863-71.

VP7: AN ATTACHMENT PROTEIN OF BLUETONGUE VIRUS FOR CELLULAR RECEPTORS IN CULICOIDES VARIIPENNIS - (Peer Reviewed Journal)

Xu, G., Wilson, W.C., Mecham, J.O., Murphy, K., Tabachnick, W.J. 1997. Vp7: an attachment protein of bluetongue virus for cellular receptors in culicoides variipennis. Journal of General Virology. 78:1617-1623.