Projects

DETECTION OF TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHY AGENTS IN LIVESTOCK, WILDLIFE, AGRICULTURAL PRODUCTS, AND THE ENVIRONMENT Appropriated (D)   Accession Number: 413072

Technologies for Detecting and Determining the Bioavailability of Bacterial Toxins Appropriated (D)   Accession Number: 421099

Immunodiagnostics to Detect Prions and Other Important Animal Pathogens Appropriated (D)   Accession Number: 424354

SIMULTANEOUS DETECTION OF MULTIPLE FOODBORNE PATHOGENS WITH A SINGLE ANTIBODY-BASED TEST Nonfunded Cooperative Agreement (N)   Accession Number: 421440

ELECTROCHEMILUMINESCENT ASSAY FOR BOTULINUM NEUROTOXINS Nonfunded Cooperative Agreement (N)   Accession Number: 419437

DEVELOP A NEW MICROBIOLOGICAL MEDIUM FOR SELECTIVE CULTURAL ENRICHMENT OF STEC Nonfunded Cooperative Agreement (N)   Accession Number: 419926

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Oxidation of methionine 216 in sheep and elk prion protein is highly dependent upon the amino acid at position 218 but is not important for prion propagation - (Peer Reviewed Journal)

Silva, C.J., Dynin, I.A., Erickson-Beltran, M.L., Requena, J.R., Balachandran, A., Onisko, B.C., Hui, C., Carter, J.M. 2013. Using mass spectrometry to detect prions and oxidized prions in scrapie-infected sheep and CWD-infected elk. Biochemistry. 52:2139-2147. doi:10.1021/bi3016795.

Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2 - (Peer Reviewed Journal)

Skinner, C.B., Mcmahon, S.A., Rasooly, R., Carter, J.M., He, X. 2013. Purification and characterization of Shiga toxin 2f, an immunologically unrelated subtype of Shiga toxin 2. PLoS One. 8(3):e59760. doi:10.1371/journal.pone.0059760.

A 7-plex microbead-based immunoassay for serotyping Shiga toxin-producing Escherichia coli - (Peer Reviewed Journal)

Clotilde, L.M., Bernard IV, C., Salvador, A., Lin, A., Lauzon, C., Muldoon, M., Xu, Y., Lindpaintner, K., Carter, J.M. 2012. A 7-plex microbead-based immunoassay for serotyping Shiga toxin-producing Escherichia coli. Journal of Microbiological Methods. 92(2):226-230. doi:10.1016/j.mimet.2012.11.023.

Detecting and discriminating among pathogenic protein conformers(prions), using mass spectrometry-based and antibody-based approaches(Abstract) - (Abstract Only)

Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson-Beltran, M.L., Hui, C., Carter, J.M. 2012. Detecting and discriminating among pathogenic protein conformers(prions), using mass spectrometry-based and antibody-based approaches(Abstract). Meeting Abstract. Poster 216.

Using synthetic small molecule reagents and antibodies to distinguish among PrP conformers: new uses for old antibodies - (Abstract Only)

Silva, C.J., Erickson-Beltran, M.L., Dynin, I.A., Carter, J.M. 2012. Using synthetic small molecule reagents and antibodies to distinguish among PrP conformers: new uses for old antibodies. Meeting Abstract. Prion 6(supplement),90.

Molecular approaches to detecting and discriminating among prions, a class of pathogenic molecules(Abstract) - (Abstract Only)

Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson-Beltran, M.L., Hui, C., Carter, J.M. 2012. Molecular approaches to detecting and discriminating among prions, a class of pathogenic molecules(Abstract). Meeting Abstract. Poster, 615.

PK-sensitive PrPSc is infectious and shares basic structural features with PK-resistant PrPSc - (Peer Reviewed Journal)

Sajnani, G., Silva, C.J., Ramos, A., Pastrana, M.A., Onisko, B.C., Erickson-Beltran, M.L., Antaki, E.M., Sigurdson, C.J., Carter, J.M., Requena, J.R. 2012. PK-sensitive PrPSc is infectious and shares basic structural features with PK-resistant PrPSc. PLoS Pathogens. 8(3):e1002547. doi:10.1371/journal.ppat.1002547.

Dynex: multiplex ELISA technology - (Peer Reviewed Journal)

Clotilde, L.M., Bernard Iv, C., Sequera, D.E., Karmali, A., Fusellier, A., Carter, J.M. 2012. Dynex: multiplex ELISA technology. Journal of Laboratory Automation. 17:309-314.2012.

Rapid O serogroup identification of the ten most clinically relevant STECs by Luminex microbead-based suspension array - (Peer Reviewed Journal)

Lin, A., Nguyen, L., Lee, T., Clotilde, L.M., Kase, J.A., Son, I., Carter, J.M., Lauzon, C.R. 2011. Rapid O serogroup identification of the ten most clinically relevant STECs by Luminex microbead-based suspension array. Journal of Microbiological Methods. 87(1):105-110. doi:10.1016/j.mimet.2011.07.019.

Using Mass Spectrometry to Diagnose Prion diseases: Can we do that? - (Abstract Only) - (16-May-11)

Diagnosing Prion Diseases: Mass Spectrometry-Based Approaches - (Abstract Only)

Silva, C.J., Erickson, M.L., Dynin, I.A., Onisko, B.C., Carter, J.M. 2011. Diagnosing Prion Diseases: Mass Spectrometry-Based Approaches. Meeting Abstract. Prion, Volume 5 (Supplement), April/May/June 2011. page 85.

Utility of mass spectrometry in the diagnosis of prion diseases - (Peer Reviewed Journal)

Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson-Beltran, M.L., Requena, J.J., Carter, J.M. 2011. Utility of mass spectrometry in the diagnosis of prion diseases. Analytical Chemistry. 83(5):1609-1615. doi:10.1021/ac102527w.

Immunoassay - (Book / Chapter)

Brandon, D.L., Carter, J.M. 2012. Immunoassay. Sun, D.W. Handbook of Food Safety Engineering. Oxford, UK:Wiley-Blackwell. P. 279-312.

Microbead-based immunoassay for simultaneous detection of Shiga toxins and isolation of Escherichia coli O157 in foods - (Peer Reviewed Journal)

Clotilde, L.M., Hartman, G.L., Lau, D.K., Carter, J.M. 2011. Microbead-based immunoassay for simultaneous detection of Shiga toxins and isolation of Escherichia coli O157 in foods. Journal of Food Protection. 74(3):373-379.

Detecting and quantifying prions: Mass spectrometry-based approaches - (Abstract Only) - (20-Apr-10)

Development of a Luminex Immunoassay for Simultaneous Detection of Shiga toxins and Escherichia coli O157 in Ground Beef - (Abstract Only)

Bernard Iv, C., Hartman, G.L., Lau, D.K., Carter, J.M., Clotilde, L.M. 2010. Development of a Luminex Immunoassay for Simultaneous Detection of Shiga toxins and Escherichia coli O157 in Ground Beef. [Abstract]. American Chemical Society National Meeting Poster #AGFD#172.

Assessing the Role of Oxidized Methionine at Position 213 in the Formation of Prions in Hamsters - (Peer Reviewed Journal)

Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson, M.L., Vensel, W.H., Requena, J., Antaki, E.M., Carter, J.M. 2010. Assessing the Role of Oxidized Methionine at Position 213 in the Formation of Prions in Hamsters. Journal of Biochemistry. (49):1854-1861.

Mass Spectrometry of Prions: Approaches to Conformational Distinction - (Abstract Only)

Silva, C.J., Onisko, B., Dynin, I.A., Erickson, M.L., Carter, J.M. 2009. Mass Spectrometry of Prions: Approaches to Conformational Distinction. [Abstract]. AOAC 123rd Annual Meeting & Exposition. S-902. p68-69

Detection of Protein Toxins - (Abstract Only)

Brandon, D.L., Cheng, L.W., He, X., Rasooly, R., Scotcher, M.C., Stanker, L.H., Carter, J.M. 2009. Detection of Protein Toxins. Meeting Abstract. Poster No. 8.

Detection and Recovery of Shiga Toxins and Escherichia Coli O157 - (Abstract Only)

Clotilde, L.M., Carter, J.M., Bernard Iv, C. 2009. Detection and Recovery of Shiga Toxins and Escherichia Coli O157. [Abstract]. Planet xMAP Luminex meeting Abstract #16.

Validation of a Cell-Free Translation Assay for Detecting Shiga Toxin 2 in Bacterial Culture - (Peer Reviewed Journal)

He, X., Quinones, B., Carter, J.M., Mandrell, R.E. 2009. Validation of a Cell-Free Translation Assay for Detecting Shiga Toxin 2 in Bacterial Culture. Journal of Agriculture and Food Chemistry. 57(11):5084-5088

Immunochemical technologies for replacement of rodent bioassays in sensitive detection of toxins in foods - (Proceedings)

Carter, J.M., Cheng, L.W., He, X., Rasooly, R., Brandon, D.L., Stanker, L.H. 2010. Immunochemical technologies for replacement of rodent bioassays in sensitive detection of toxins in foods. Proceedings from Biodetection Technologies 6th Edition. 5:75-95.

Prion infected Meat-and-Bone Meal is still infectious after biodiesel production - (Peer Reviewed Journal)

Bruederle, C.E., Hnasko, R.M., Kraemer, T., Garcia, R.A., Haas, M.J., Marmer, W.N., Carter, J.M. 2008. Prion infected Meat-and-Bone Meal is still Infectious After Biodiesel Production. PLoS One 3(8): e2969. doi:10.1371/journal.pone.0002969.

Effect of food matrices on the biological activity of ricin - (Peer Reviewed Journal)

He, X., Lu, S.S., Cheng, L.W., Rasooly, R., Carter, J.M. 2008. Effect of food matrices on the biological activity of ricin. Journal of Food Protection 71(10):2053-2058.

Detection of botulinum neurotoxin-A activity in food by peptide cleavage assay - (Peer Reviewed Journal)

Rasooly, R., Stanker, L.H., Carter, J.M., Do, P.M., Cheng, L.W., He, X., Brandon, D.L. 2008. In vitro peptide cleavage assay for detection of Botulinum Neurotoxin-A activity in food. International Journal of Food Microbiiology. 126 (2008):135-139

Effects of purification on the bioavailability of botulinum neurotoxin type A - (Peer Reviewed Journal)

Cheng, L.W., Onisko, B.C., Johnson, E.A., Reader, J.R., Griffey, S.M., Larson, A.E., Tepp, W.H., Stanker, L.H., Brandon, D.L., Carter, J.M. 2008. Effects of purification on the bioavailability of botulinum neurotoxin type A. Toxicology. 249(2008):123-129.

Mass Spectrometric Approaches to Detecting and Quantifying Prions - (Abstract Only)

Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson, M.L., Carter, J.M. 2008. Mass Spectrometric Approaches to Detecting and Quantifying Prions. [Abstract]. Third International CWD Symposium. Advancing the Science and Developing the Tools. Vol 3. p23.

Sensitive, Preclinical Detection of Prions in Brain by nanospray liquid chromatography/tandem mass spectrometry - (Peer Reviewed Journal)

Onisko, B.C., Silva, C.J., Dynin, I.A., Erickson, M.L., Vensel, W.H., Hnasko, R.M., Requena, J.R., Carter, J.M. 2007. Sensitive, Preclinical Detection of Prions in Brain by nanospray liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry. 21(24):4023-4026.

Sonication Induced Intermediate in Prion Protein Conversion - (Peer Reviewed Journal)

Zukas, A.A., Bruederle, C.E., Carter, J.M. 2008. Sonication Induced Intermediate in Prion Protein Conversion. Protein and Peptide Letters. 53(2):206-211

Application of a real time PCR method to detect castor toxin contamination in fluid milk and eggs - (Peer Reviewed Journal)

He, X., Carter, J.M., Brandon, D.L., Cheng, L.W., Mckeon, T.A. 2007. Application of a real time PCR method to detect castor toxin contamination in fluid milk and eggs. Journal of Agricultural and Food Chemistry. 55:6897-6902

Mass Spectrometric Detection of Attomole Amounts of the Prion Protein by nanoLC-MS-MS - (Peer Reviewed Journal)

Onisko, B.C., Dynin, I.A., Requena, J.R., Silva, C.J., Erickson, M.L., Carter, J.M. 2007. Mass Spectrometric Detection of Attomole Amounts of the Prion Protein by nanoLC-MS-MS. Journal of American Society for Mass Spectrometry. Volume(18); 1070-1079

Molecular Approaches for the Identification of Toxin in Foods - (Abstract Only) - (23-Feb-07)

Detection of castor contamination by real-time polymerase chain reaction - (Peer Reviewed Journal)

He, X., Brandon, D.L., Chen, G.Q., McKeon, T.A., Carter, J.M. 2007. Detection of castor contamination by real-time polymerase chain reaction. Journal of Agricultural and Food Chemistry. 55(2):545-550.

A COFACTOR LIMITS THE PMCA CONVERSION REACTION - (Abstract Only)

Zukas, A.A., Carter, J.M. 2006. A cofactor limits the pmca conversion reaction [Abstract]. Strategies, advances and trends towards protection of society. p 290.

PV-1 LABELS TRANS-CELLULAR OPENINGS IN MOUSE ENDOTHELIAL CELLS AND IS NEGATIVELY REGULATED BY VEGF - (Peer Reviewed Journal)

Hnasko, R.M., Carter, J.M., Medina, F., Lisanti, M. 2006. Pv-1 labels trans-cellular openings in mouse endothelial cells and is negatively regulated by vegf. Cell Cycle. 5(17):2021-28.

SONICATION INDUCED INTERMEDIATE IN PRION CONVERSION - (Abstract Only)

Zukas, A.A., Carter, J.M. 2006. Sonication induced intermediate in prion conversion. [Abstract]. ACS National Meeting. Poster BIOL55

BIOAVAILABILITY OF CRUDE RICIN IN FOOD MATRICES - (Abstract Only)

Cheng, L.W., Brandon, D.L., He, X., Chen, G.Q., Carter, J.M. 2006. Bioavailability of crude ricin in food matrices. [Abstract]. ACS National Meeting and Exposition. Poster AGFD 64

DEVELOPMENT OF NEW MONOCLONAL ANTIBODIES SPECIFIC FOR BOTULINUM NEUROTOXIN TYPE A - (Abstract Only)

Stanker, L.H., Merrill, P.A., Cheng, L.W., Carter, J.M., Brandon, D.L. 2006. Development of new monoclonal antibodies specific for botulinum neurotoxin type a. [Abstract]. ACS National Meeting. Oral Presentation AGRO 235.

DEVELOPMENT OF MONOCLONAL ANTIBODIES SPECIFIC FOR RICIN - (Abstract Only)

Brandon, D.L., Cheng, L.W., He, X., Carter, J.M. 2006. Development of monoclonal antibodies specific for ricin. [Abstract]. ACS National Meeting and Exposition. Oral Presentation AGRO 234

MASS SPECTROMETRIC DETECTION OF ATTOMOLE AMOUNTS OF THE PRION PROTEIN, PRP 27-30, BY NANOLC-MS-MS - (Abstract Only)

Onisko, B.C., Requena, J., Silva, C.J., Dynin, I.A., Carter, J.M. 2006. Mass spectrometric detection of attomole amounts of the prion protein, prp 27-30, by nanolc-ms-ms. [Abstract]. 232nd ACS National Meeting and Exposition. Poster AGFD 200

DETECTION OF CASTOR CONTAMINATION IN GROUND BEEF BY REAL-TIME PCR - (Abstract Only)

He, X., Brandon, D.L., Chen, G.Q., Mckeon, T.A., Carter, J.M. 2006. Detection of castor contamination in ground beef by real-time pcr. [Abstract]. ACS National Meeting and Exposition. Poster AGFD 18.

STRAIN-SPECIFIC PRP-RES IN VITRO AMPLIFICATION BY THE PMCA TECHNIQUE - (Abstract Only)

Zukas, A.A., Carter, J.M. 2006. Strain-specific prp-res in vitro amplification by the pmca technique. [Abstract]. Cambridge Healthtec Institute, Transmissible Spongiform Encephalopathies. Poster #12.

NEW RESEARCH AT THE USDA ARS ON DETECTION OF CRUDE BOTULINUM NEUROTOXIN IN FOOD - (Abstract Only)

Carter, J.M., Binder, R.G., Stanker, L.H., Brandon, D.L. 2005. New research at the usda ars on detection of crude botulinum neurotoxin in food. [Abstract]. Interagency Botulism Research Committee. Poster 4.

PRION RESEARCH AT THE WESTERN REGIONAL RESEARCH CENTER - (Abstract Only)

Carter, J.M., Onisko, B., Silva, C., Stanker, L. Requena, J.R., 2005. Prion research at the Western Regional Research Center [abstract]. International Symposium on the New Prion Biology: Basic Science, Diagnosis and Therapy, April 7-9, 2005, Venice, Italy. Poster P17.

TECHNIQUES AND CHALLENGES IN THE DIAGNOSIS OF PRION DISEASES. - (Abstract Only)

Stanker, L.H., Carter, J.M. 2004. Techniques and challenges in the diagnosis of prion diseases.. Meeting Abstract.