Projects

ENGINEERING ENZYMATIC REDIRECTION OF NATURAL CROP OIL PRODUCTION TO INDUSTRIAL OIL PRODUCTION Appropriated (D)   Accession Number: 418098

TUNG SEED OIL BODY PROTEOMICS Nonfunded Cooperative Agreement (N)   Accession Number: 420768

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Molecular properties of the class III subfamily of acyl-coenyzme A binding proteins from tung tree (Vernicia fordii) - (Peer Reviewed Journal)

Pastor, S., Sethumadhavan, K., Ullah, A.H.J., Gidda, S., Cao, H., Mason, C., Chapital, D., Scheffler, B., Mullen, R., Dyer, J., Shockey, J. 2013. Molecular properties of the class III subfamily of acyl-coenyzme A binding proteins from tung tree (Vernicia fordii). Plant Science. 203-204:79-88.

Comparison of TaqMan and SYBR Green qPCR methods for quantitative gene expression in tung tree tissues - (Peer Reviewed Journal)

Cao, H., Shockey, J.M. 2012. Comparison of TaqMan and SYBR Green qPCR methods for quantitative gene expression in tung tree tissues. Journal of Agricultural and Food Chemistry. 60:12296-12303.

Measuring phosphatidic acid phosphatase (EC 3.1.3.4) activity using two phosphomolybdate-based colorimetric methods - (Peer Reviewed Journal)

Ullah, A.H.J., Sethumadhavan, K., Shockey, J. 2012. Measuring phosphatidic acid phosphatase (EC 3.1.3.4) activity using two phosphomolybdate-based colorimetric methods. Advances in Biological Chemistry. 2:416-421.

Purification, characterization, and bioinformatics studies of phosphatidic acid phosphohydrolase from Lagenaria siceraria - (Peer Reviewed Journal)

Ullah, A.H.J., Sethumadhavan, K., Grimm, C., Shockey, J. 2012. Purification, characterization, and bioinformatics studies of phosphatidic acid phosphohydrolase from Lagenaria siceraria. Advances in Biological Chemistry. 2(4):403-410.

Expression and purification of recombinant tung tree diacylglycerol acyltransferase 2 - (Peer Reviewed Journal) - (23-Dec-11)

Expression and purification of recombinant tung tree diacylglycerol acyltransferase 2 - (Peer Reviewed Journal)

Cao, H., Chapital, D.C., Howard Jr, O.D., Deterding, L.J., Mason, C.B., Shockey, J.M., Klasson, K.T. 2012. Expression and purification of recombinant tung tree diacylglycerol acyltransferase 2. Applied Microbiology and Biotechnology. 96(3):711-727.

Expression of a lipid-inducible, self-regulating form of Yarrowia lipolytica lipase LIP2 in Saccharomyces cerevisiae - (Peer Reviewed Journal)

Shockey, J., Chapital, D., Gidda, S., Mason, C., Davis, G., Klasson, K., Cao, H., Mullen, R., Dyer, J. 2011. Expression of a lipid-inducible, self-regulating form of Yarrowia lipolytica lipase LIP2 in Saccharomyces cerevisiae. Applied Microbiology and Biotechnology. 92(6):1207-1217.

Expression of tung tree diacylglycerol acyltransferase 1 in E. coli - (Peer Reviewed Journal)

Cao, H., Chapital, D.C., Shockey, J.M., Klasson, K.T. 2011. Expression of tung tree diacylglycerol acyltransferase 1 in E. coli. BMC Biotechnology. 11:73 (13 pages).

Bioengineering recombinant tung tree diacylglycerol acyltransferases - (Abstract Only)

Cao, Heping, Chapital, Dorselyn C., Howard, Jr., O.D., Shockey, Jay M., Klasson, K. Thomas. 2011. Bioengineering recombinant tung tree diacylglycerol acyltransferases (abstract). Presented at the Society for Industrial Microbiology and Biotechnology Annual Meeting and Exhibition in New Orleans, Louisiana, July 24-28. http://sim.confex.com/sim/2011/webprogram/Paper19213.html.

Expression and purification of recombinant tung tree diacylglycerol acyltransferase 2 - (Abstract Only)

Howard, O., Chapital, D.C., Shockey, J.M., Cao, H. 2011. Expression and purification of recombinant tung tree diacylglycerol acyltransferase 2 (abstract). Southern Section of the American Society of Plant Biologists. p. 5.

Purification of recombinant tung tree diacylglycerol acyltransferases from E. coli - (Abstract Only)

Cao, H., Chapital, D.C., Howard Jr, O.D., Jiang, X., Shockey, J.M., Klasson, K.T. 2011. Purification of recombinant tung tree diacylglycerol acyltransferases from E. coli (abstract). Journal of the Federation of American Societies for Experimental Biology. 25:765.8.

Genome-level and biochemical diversity of the acyl-activating enzyme superfamily in plants - (Peer Reviewed Journal)

Shockey, J., Browse, J. 2011. Genome-level and biochemical diversity of the acyl-activating enzyme superfamily in plants. Plant Journal. (66):143-160.

Hydrophobic-domain-dependent protein-protein interactions mediate the localization of GPAT enzymes to ER subdomains - (Peer Reviewed Journal)

Gidda, S.K., Shockey, J.M., Rothstein, S.J., Dyer, J.M., Mullen, R.T. 2011. Hydrophobic-domain-dependent protein-protein interactions mediate the localization of GPAT enzymes to ER subdomains. Traffic. 12:452-472.

Castor phospholipid:diacylglycerol acyltransferase facilitates efficient metabolism of hydroxy fatty acids in transgenic Arabidopsis - (Peer Reviewed Journal)

Van Erp, H., Bates, P.D., Burgal, J., Shockey, J., Browse, J. 2011. Castor phospholipid:diacylglycerol acyltransferase facilitates efficient metabolism of hydroxy fatty acids in transgenic Arabidopsis. Plant Physiology. 155:683-693.

The N termini of Brassica and tung omega-3 fatty acid desaturases mediate proteasome-dependent protein degradation in plant cells - (Peer Reviewed Journal)

Khuu, N., Gidda, S., Shockey, J.M., Dyer, J.M., Mullen, R.T., 2011. The N termini of Brassica and tung omega-3 fatty acid desaturases mediate proteasome-dependent protein degradation in plant cells. Plant Signaling and Behavior. 6(3):422-425.

Mining the bitter melon (momordica charantia l.) seed transcriptome by 454 analysis of non-normalized and normalized cDNA populations for conjugated fatty acid metabolism-related genes - (Peer Reviewed Journal)

Yang, P., Li, X., Shipp, M.J., Shockey, J.M., Cahoon, E.B. 2010. Mining the bitter melon (momordica charantia l.) seed transcriptome by 454 analysis of non-normalized and normalized cDNA populations for conjugated fatty acid metabolism-related genes. Biomed Central (BMC) Plant Biology. 10:250.

Transmembrane domain-dependent protein-protein interactions participate in the localization of GPAT enzymes to ER subdomains - (Abstract Only)

Gidda, S.K., Shockey, J.M., Rothstein, S.J., Dyer, J.M., and Mullen, R.T. (2010). Transmembrane domain-dependent protein-protein interactions participate in the localization of GPAT enzymes to ER subdomains. Joint Annual Meeting of the American Society of Plant Biologists & the Canadian Society of Plant Physiologists, July 31 - August 4, 2010, Montreal, Canada.

Expression of tung seed diacylglycerol acyltransferases (DGAT) in E. coli and yeast - (Abstract Only)

Cao, H., Chapital, D.C., Mason, C.B., Shockey, J.M., Klasson, K.T. 2010. Expression of tung seed diacylglycerol acyltransferases (DGAT) in E. coli and yeast (abstract). Plant Biology 2010, July 31-August 4, Montreal, Canada.

Temperature-sensitive, Post-translational Regulation of Plant Omega-3 Fatty-acid Desaturases is Mediated by the Endoplasmic Reticulum-associated Degradation Pathway - (Peer Reviewed Journal)

O'Quin, J.B., Bourassa, L., Zhang, D., Shockey, J.M., Gidda, S.K., Fosnot, S., Chapman, K.D., Mullen, R.T., Dyer, J.M. 2010. Temperature-sensitive, Post-translational Regulation of Plant Omega-3 Fatty-acid Desaturases is Mediated by the Endoplasmic Reticulum-associated Degradation Pathway. Journal of Biological Chemistry. 285(28):21781-21796.

Biogenesis of ER subdomains containing DGAT2, an enzyme involved in industrial oil biosynthesis - (Abstract Only)

Gidda, S.K., Shockey, J.M., Mullen, R.T., and Dyer, J.M. (2010). Biogenesis of ER subdomains containing DGAT2, an enzyme involved in industrial oil biosynthesis. Annual Meeting of the American Oil Chemists' Society, May 11 - 16, 2010, Phoenix, AZ.

TEMPERATURE-SENSITIVE, POST-TRANSLATIONAL REGULATION OF PLANT OMEGA-3 FATTY ACID DESATURASES IS MEDIATED BY THE ER-ASSOCIATED DEGRADATION PATHWAY - (Abstract Only)

Zhang, D., Quin, J.B., Bourassa, L., Foss, S., Shockey, J.M., Gidda, S.K., Chapman, K.D., Mullen, R.T., and Dyer, J.M. (2010). Temperature-sensitive, post-translational regulation of plant omega-3 fatty acid desaturases is mediated by the ER-associated degradation pathway. Annual Meeting of the American Society of Biochemistry and Molecular Biology, April 24 - 28, 2010, Anaheim, CA.

Organ fusion and defective cuticle function in a lacs1 lacs2 double mutant of Arabidopsis - (Peer Reviewed Journal)

Weng, H., Molina, I., Shockey, J., Browse, J. 2010. Organ fusion and defective cuticle function in a lacs1 lacs2 double mutant of Arabidopsis. Planta. 231(5):1089-1100.

Biogenesis of glycerol 3-phosphate acyltransferase (GPAT): influence of transmembrane domains and protein-protein interactions on the localization of GPAT to ER subdomains - (Abstract Only)

Gidda, S.K., Shockey, J.M., Dyer, J.M., and Mullen, R.T. (2009). Biogenesis of glycerol 3-phosphate acyltransferase (GPAT): influence of transmembrane domains and protein-protein interactions on the localization of GPAT to ER subdomains. Eastern Regional Meeting of the Canadian Society of Plant Physiologists, December 4 - 5, 2009, Guelph, Ontarion, Canada.

Arabidopsis thaliana GPAT8 and GPAT9 are localized to the ER and possess distinct ER retrieval signals: functional divergence of the dilysine ER retrieval motif in plant cells - (Peer Reviewed Journal)

Gidda, S.K., Shockey, J.M., Rothstein, S.J., Dyer, J.M., Mullen, R.T. (2009). Arabidopsis thaliana GPAT8 and GPAT9 are localized to the ER and possess distinct ER retrieval signals: functional divergence of the dilysine ER retrieval motif in plant cells. Plant Physiology and Biochemistry, 47:867-879.

Arabidopsis thaliana GPAT8 and GPAT9 are localized to the ER and possess distinct ER retrieval signals: Functional divergence of the dilysine ER retrieval motif in plant cells - (Peer Reviewed Journal)

Gidda, S.K., Shockey, J.M., Rothstein, S.J., Dyer, J.M., Mullen, R.T. 2009. Arabidopsis thaliana GPAT8 and GPAT9 are localized to the ER and possess distinct ER retrieval signals: Functional divergence of the dilysine ER retrieval motif in plant cells. Plant Physiology and Biochemistry. 47(10):867-879.

Engineering industrial oil biosynthesis: cloning and characterization of Kennedy pathway acyltransferases from novel oilseed species - (Book / Chapter)

Shockey, J.M. 2009. Engineering industrial oil biosynthesis: cloning and characterization of Kennedy pathway acyltransferases from novel oilseed species. In: Hou, T.H., Shaw, J.-F., editors. Biocatalysis and Agricultural Biotechnology. Abingdon, England:CRC Press. pp. 19-31.

Metabolic engineering of hydroxy fatty acid production in plants: rcdgat2 drives dramatic increases in ricinoleate levels in seed oil - (Peer Reviewed Journal)

Burgal, J., Shockey, J., Lu, C., Dyer, J., Larson, T., Graham, I., Browse, J. 2008. Metabolic engineering of hydroxy fatty acid production in plants: rcdgat2 drives dramatic increases in ricinoleate levels in seed oil. Plant Biotechnology Journal. 6(8):819-831.

Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: solving bottlenecks in fatty acid flux - (Review Article)

Cahoon, E.B., Shockey, J.M., Dietrich, C.R., Dyer, J.M. 2007. Engineering oilseeds for sustainable production of industrial and nutritional feedstocks: solving bottlenecks in fatty acid flux. Current Opinion in Plant Biology. 10:236-244.

CHARACTERIZATION IN VITRO AND IN VIVO OF THE PUTATIVE MULTIGENE 4-COUMARATE:COA LIGASE NETWORK IN ARABIDOPSIS: SYRINGYL LIGNIN AND SINAPATE/SINAPYL ALCOHOL DERIVATIVE FORMATION. - (Peer Reviewed Journal)

Costa, M.A., Bedgar, D.L., Moinuddin, S.G., Kim, K.W., Cardenas, C.L., Cochrane, F.C., Shockey, J.M., Helms, G.L., Amakura, Y., Takahashi, H., Milhollan, J.K., Davin, L.B., Browse, J., Lewis, N.G. 2005. Characterization in vitro and in vivo of the putative multigene 4-coumarate:coa ligase network in arabidopsis: syringyl lignin and sinapate/sinapyl alcohol derivative formation. Phytochemistry. 66:2072-2091.

TYPE 1 AND TYPE 2 DIACYLGLYCEROL ACYLTRANSFERASES FROM DEVELOPING SEEDS OF TUNG (VERNICIA FORDII) DISPLAY DIFFERENT AFFINITIES FOR ELEOSTEARIC ACID-CONTAINING SUBSTRATES. - (Abstract Only)

Shockey, J.M., Gidda, S., Chapital, D.C., Kuan, J.-C.W., Rothstein, S.J., Mullen, R.T., and Dyer, J.M. 2005. Type 1 and type 2 diacylglycerol acyltransferases from developing seeds of tung (Vernicia fordii) display different affinities for eleostearic acid-containing substrates (abstract). 2005 Biochemistry and Molecular Biology of Plant Fatty Acids and Glycerolipids Symposium, Abstract C3, page 11, June 10-14, Fallen Leaf Lake, CA. 2005:22

ER-LOCALIZED OMEGA-3 FATTY ACID DESATURASES ARE SHORT-LIVED PROTEINS THAT CONTAIN DEGRADATION SIGNALS IN THEIR N TERMINI - (Abstract Only)

Dyer, J.M., Bryan, J., Bourassa, L., Shockey, J.M., and Mullen, R.T. 2005. ER-localized omega-3 fatty acid desaturases are short-lived proteins that contain degradation signals in their N termini (abstract). 2005 Biochemistry and Molecular Biology of Plant Fatty Acids and Glycerolipids Symposium, Abstract A3, page 6, June 10-14, Fallen Leaf Lake, CA. 2005:17

CLONING, FUNCTIONAL ANALYSIS, AND SUBCELLULAR LOCALIZATION OF TWO ISOFORMS OF NADH:CYTOCHROME B5 REDUCTASE FROM DEVELOPING SEEDS OF TUNG (VERNICIA FORDII) - (Peer Reviewed Journal)

Shockey, J.M., Dhanoa, P.K., Dupuy, T., Chapital, D.C., Mullen, R.T., and Dyer, J.M. 2005. Cloning, functional analysis, and subcellular localization of two isoforms of NADH:cytochrome b5 reductase from developing seeds of tung (Vernicia fordii). Plant Science. 169(2):375-385.

BIOTRANSFORMATION OF LIPIDS IN YEAST CELLS: ENZYMATIC CONVERSION OF FATTY ACIDS INTO OMEGA-3 AND CONJUGATED FATTY ACIDS FOR POTENTIAL USAGE AS NUTRACEUTICALS - (Proceedings)

Dyer, J.M., Shockey, J.M., Mullen, R.T., Chapital, D.C., Kuan, J.-C., and Stewart, G. (2004). Biotransformation of lipids in yeast cells: enzymatic conversion of fatty acids into omega-3 and conjugated fatty acids for potential usage as nutraceuticals. In: Proceedings of the 33rd Annual United States-Japan Cooperative Program in Natural Resources Annual Meeting, December 11, 2004, Tokyo, JA. p. 210-214.

CLONING AND CHARACTERIZATION OF A LYSOPHOSPHATIDIC ACID ACYLTRANSFERASE FROM TUNG (ALEURITIES FORDII) - (Abstract Only)

Shockey, J.M., Dyer, J.M., Chapital, D.C., Kuan, J.W., Mullen, R.T., Cary, J.W. 2004. Cloning and characterization of a lysophosphatidic acid acyltransferase from tung (aleurities fordii)(abstract). American Society of Microbiology.

BIOSYNTHESES OF TRIACYLGLYCEROLS CONTAINING ELEOSTEARIC ACID. CLONING AND CHARACTERIZATION OF THE QUANTITATIVE AND QUALITATIVE ROLES OF DIACYLGLYCEROL ACYLTRANSFERASE AND PHOSPHOLIPID-DIACYLGLYCEROL ACYLTRANSFERASE FROM TUNG - (Abstract Only)

Shockey, J.M., Chapital, D.C., Kuan, J.W., Mullen, R.T., Dupuy, T., Dyer, J.M. 2004. Biosyntheses of triacylglycerols containing eleostearic acid. cloning and characterization of the quantitative and qualitative roles of diacylglycerol acyltransferase and phospholipid-diacylglycerol acyltransferase from tung (abstract). American Society of Microbiology.

ENGINEERING HIGH LEVELS OF ELEOSTEARIC ACID CONTENT IN OILSEEDS - CLONING AND FUNCTIONAL CHARACTERIZATION OF TRIACYLGLYCEROL BIOSYNTHETIC ACYLTRANSFERASE GENES FROM TUNG (ALEURITES FORDII) - (Abstract Only)

Shockey, J.M., Chapital, D.C., Kuan, J.W., Mullen, R.T., Dyer, J.M. 2004. Engineering high levels of eleostearic acid content in oilseeds - cloning and functional characterization of triacylglycerol biosynthetic acyltransferase genes from tung (aleurites fordii) (abstract). American Society of Plant Biology.

THE ACYL-COENZYME A SYNTHETASE ENCODED BY LACS2 IS ESSENTIAL FOR NORMAL CUTICLE DEVELOPMENT IN ARABIDOPSIS. - (Peer Reviewed Journal)

Schnurr, J., Shockey, J.M., Browse, J. 2004. The acyl-coenzyme a synthetase encoded by lacs2 is essential for normal cuticle development in arabidopsis. The Plant Cell. 16:629-642.

BIOCHEMICAL AND MOLECULAR CHARACTERIZATION OF ACH2, AN ACYL-COA THIOESTERASE FROM ARABIDOPSIS THALIANA. - (Peer Reviewed Journal)

Tilton, G.B., Shockey, J.M., Browse, J. 2003. Biochemical and molecular characterization of ach2, an acyl-coa thioesterase from arabidopsis thaliana. Journal of Biological Chemistry. 279:(9)7487-7494.