Projects

Improving Biochemical Processes for the Production of Sustainable Fuels and Chemicals Appropriated (D)   Accession Number: 415148

Automated Engineering of Lipase Enzymes for Covalent Attachment to Resin and Identification of Best Transesterification Nonfunded Cooperative Agreement (N)   Accession Number: 415293

Publications

Current Status and Future Considerations Regarding the US Biofuel Industry - (Abstract)

Hughes, S.R., Tasaki, K., Rich, J.O. 2009. Current Status and Future Considerations Regarding the US Biofuel Industry [abstract]. Mitsubish Chemical Corporation.

Cloning and Expression of Laccase from Trametes versicolor in Saccharomyces cerevisiae using a Novel Vector System - (Abstract)

Pinkelman, R.J., Hughes, S.R., Choi, H., Bang, S.S. 2009. Cloning and Expression of Laccase from Trametes versicolor in Saccharomyces cerevisiae using a Novel Vector System [abstract]. American Institute for Chemical Engineers.

Production of the Anaerobic GMAX-L Yeast Using High-Throughput Mating and Transformation of Saccharomyces cerevisiae With Identified Genes For Simultaneous Cellulosic Ethanol and Biodiesel Production - (Abstract)

Hughes, S.R., Rich, J.O., Moser, B.R., Doll, K.M., Bischoff, K.M., Qureshi, N., Jones, M., Tasaki, K. 2009. Production of the Anaerobic GMAX-L Yeast Using High-Throughput Mating and Transformation of Saccharomyces cerevisiae With Identified Genes For Simultaneous Cellulosic Ethanol and Biodiesel Production [abstract]. Bradley Biology Chemisty Seminar Series.

Development of Thermotolerant Consolidated Bioprocessing Microbes for Cost-efficient Cellulosic Ethanol Production - (Abstract)

Christopher, L.P., Gibbons, W., Bleakeley, B., West, T., Hughes, S.R., Sani, R.K., Bang, S.S. 2009. Development of Thermotolerant Consolidated Bioprocessing Microbes for Cost-efficient Cellulosic Ethanol Production [abstract]. Industrial Biotechnology and Bioprocessing. Talk #4. p. 1.

Industrial Saccharomyces cerevisiae Yeast Strain Engineered to Convert Glucose, Mannose, Arabinose, and Xylose (GMAX) to Ethanol Anaerobically - (Abstract)

Hughes, S.R., Qureshi, N., Butt, T., Bang, S., Bischoff, K.M., Saha, B.C., Liu, S., Rich, J.O. 2009. Industrial Saccharomyces cerevisiae Yeast Strain Engineered to Convert Glucose, Mannose, Arabinose, and Xylose (GMAX) to Ethanol Anaerobically [abstract]. American Institute of Chemical Engineers.

Gmax Yeast Background Strain Made from Industrial Tolerant Saccharomyces Cerevisiae Engineered to Convert Sucrose, Starch and Cellulosic Sugars Universally to Ethanol Anaerobically with Concurrent Coproduct Expression - (Abstract)

Hughes, S.R., Butt, T., Boiley, J., Bang, S., Gibbons, W., Bischoff, K.M. 2009. GMAX Yeast Background Strain made from Industrial Tolerant Saccharomyces cerevisiae Engineered to Convert Sucrose, Starch and Cellulosic Sugars Universally to Ethanol Anaerobically with Concurrent Coproduct Expression [abstract]. Society for In vitro Biology. Abstract #106. p. 135.

Gmax-L Saccharomyces Cerevisiae Strains for Profitable Sustainable Cellulosic Ethanol and Biodiesel Production Concurrently Using Engineered Workcell - (Abstract)

Hughes, S.R., Moser, B.R., Doll, K.M., Tasaki, K., Harmsen, A., Jones, M., Bang, S., Gibbons, W., Butt, T. 2009. GMAX-L Saccharomyces Cerevisiae Strains for Profitable Sustainable Cellulosic Ethanol and Biodiesel Production Concurrently using Engineered Workcell [abstract]. Center for Process Analytical Chemisty. Abstract No. 2. p. 1.

Characterization of Extremophilic Cellulose-Degrading Bacteria from the Deep Subsurface of the Homestake Gold Mine, Lead, South Dakota, USA - (Abstract)

Rastogi, G., Muppidi, G., Gurram, R., Adhikari, A., Bang, S., Christopher, L., Bischoff, K.M., Hughes, S.R., Apel, W., Sani, R.K. 2009. Characterization of Extremophilic Cellulose-Degrading Bacteria from the Deep Subsurface of the Homestake Gold Mine, Lead, South Dakota [abstract]. Symposium on Biotechnology for Fuels and Chemicals. Poster No. 6-11. p. 131.

Gmax Yeast Background Strain Made from Industrial Tolerant Saccharomyces Cerevisiae Engineered to Convert Pretreated Lignocellulosic Starch and Cellulosic Sugars Universally to Ethanol Anaerobically - (Abstract)

Hughes, S.R., Tasaki, K., Moser, B., Doll, K., Butt, T., Sterner, D., Bischoff, K., Hector, R., Jones, M., and Bang, S. 2009. GMAX Yeast Background Strain made from Industrial Tolerant Saccharomyces cerevisiae Engineered to Convert Pretreated Lignocellulosic Starch and Cellulosic Sugars Universally to Ethanol Anaerobically [abstract]. Cambridge Healthtech Institute, Advanced Biofuels Development Summit. Abstract No. 2. p. 3.

Historical Perspective of Biofuels: Learning from the Past to Rediscover the Future - (Peer Reviewed Journal)

Songstad, D.D., Lakshmanan, P., Chen, J., Gibbons, W., Hughes, S.R., Nelson, R. 2009. Historical Perspective of Biofuels: Learning from the Past to Rediscover the Future. In Vitro Cellular and Developmental Biology - Plants. 45:189-192.

Integrated Biorefineries with Engineered Microbes and High-value Co-products for Profitable Biofuels Production - (Review Article)

Gibbons, W.R., Hughes, S.R. 2009. Integrated Biorefineries with Engineered Microbes and High-value Co-products for Profitable Biofuels Production. In Vitro Cellular and Developmental Biology - Plants. 45:218-228.

Automated High Throughput GMAX-L Strains of Saccharomyces Cereviciae for Profitable Cellulosic Ethanol Production from Industrial Hydrosylates - (Abstract)

Hughes, S.R., Tasaki, K., Doll, K.M., Moser, B.R., Rich, J.O., Qureshi, N., Hector, R.E., Dien, B.S., Cotta, M.A., Bischoff, K.M., Liu, S., Bang, S., Jones, M. 2009. Automated High Throughput GMAX-L Strains of Saccharomyces Cereviciae for Profitable Cellulosic Ethanol Production from Industrial Hydrosylates [abstract]. Society for In-Vitro Biology. p. 122.

Automated Yeast Transformation Protocol to Engineer S. cerevisiae Strains for Cellulosic Ethanol Production with Open Reading Frames that Express Proteins Binding to Xylose Isomerase Identified using Robotic Two-hybrid Screen - (Peer Reviewed Journal)

Hughes, S.R., Rich, J.O., Bischoff, K.M., Hector, R.E., Qureshi, N., Saha, B.C., Dien, B.S., Liu, S., Jackson Jr, J.S., Sterner, D.E., Butt, T.R., Labaer, J., Cotta, M.A. 2009. Automated Yeast Transformation Protocol to Engineer S. cerevisiae Strains for Cellulosic Ethanol Production with Open Reading Frames that Express Proteins Binding to Xylose Isomerase Identified using Robotic Two-hybrid Screen. Journal of the Association for Laboratory Automation. 8:200-212.

Automated production GMAX-L strains of Saccharomyces cereviciae for profitable sustainable cellulosic ethanol production combined with valuable coproduct isolation in mixed biorefinery settings - (Abstract)

Hughes, S.R., Doll, K., Moser, B., Bang, S., Rich, J., Qureshi, N., Hector, R., Dien, B., Cotta, M., Bischoff, K., Liu, S., Jones, M. 2009. Automated Production GMAX-L Strains of Saccharomyces cereviciae for Profitable Sustainable Cellulosic Ethanol Production Combined with Valuable Coproduct Isolation in Mixed Biorefinery Settings [abstract]. LabAutomation 2009. Abstract No. 109. p. 42.

Creating libraries for commercial yeast strains through miniaturization of cloning and transformations using the BioRAPTR FRD Microfluidic workstation - (Abstract)

Yoder, A., Pajak, L., Jackson Jr, J.S., Hughes, S.R. 2009. Creating Libraries for Commercial Yeast Strains through Miniaturization of Cloning and Transformations using the BioRAPTR FRD Microfluidic Workstation [abstract]. LabAutomation. Poster #12. p. 49.

Isolation and Characterization of Cellulose-degrading Bacteria from the Deep Subsurface of the Homestake Gold Mine, Lead, South Dakota, USA - (Peer Reviewed Journal)

Rastogi, G., Muppidi, G.L., Gurram, R.N., Adhikari, A., Bischoff, K.M., Hughes, S.R., Apel, W.A., Bang, S.S., Dixon, D.J., Sani, R.K. 2009. Isolation and Characterization of Cellulose-degrading Bacteria from the Deep Subsurface of the Homestake Gold Mine, Lead, South Dakota, USA. Journal of Industrial Microbiology and Biotechnology. 36:585-598.

Automated Yeast Mating Protocol Using Open Reading Frames from Saccharomyces cerevisiae Genome to Improve Yeast Strains for Cellulosic Ethanol Production - (Peer Reviewed Journal)

Hughes, S.R., Hector, R.E., Rich, J.O., Qureshi, N., Bischoff, K.M., Dien, B.S., Saha, B.C., Liu, S., Jackson Jr, J.S., Sterner, D.E., Butt, T.R., Labaer, J., Cotta, M.A. 2009. Automated Yeast Mating Protocol Using Open Reading Frames from Saccharomyces cerevisiae Genome to Improve Yeast Strains for Cellulosic Ethanol Production. Journal of the Association for Laboratory Automation. 8:190-199.

Extracellular Hemicellulolytic Enzymes from the Maize Endophyte Acremonium zeae - (Peer Reviewed Journal)

Bischoff, K.M., Wicklow, D.T., Jordan, D.B., De Rezende, S.T., Liu, S., Hughes, S.R., Rich, J.O. 2009. Extracellular Hemicellulolytic Enzymes from the Maize Endophyte Acremonium zeae. Current Microbiology. 58:499-503.

Make use of lactic acid bacteria in biomass to biofuel - (Abstract)

Liu, S., Bischoff, K.M., Leathers, T.D., Hughes, S.R., Rich, J.O. 2008. Make Use of Lactic Acid Bacteria in Biomass to Biofuel [abstract]. International Symposium on Biocatalysis and Biotechnology. Post No. M-2. p. 60.

Conversion of Biomass Hydrolysates and Other Substrates to Ethanol and Other Chemicals by Lactobacillus buchneri - (Peer Reviewed Journal)

Liu, S., Bischoff, K.M., Hughes, S.R., Leathers, T.D., Price, N.P., Qureshi, N., Rich, J.O. 2009. Conversion of Biomass Hydrolysates and Other Substrates to Ethanol and Other Chemicals by Lactobacillus buchneri. Letters of Applied Microbiology. 48(3):337-342.

Three-vector System for use on Automated Systems for High-level Functional Expression of Value-added Co-products with Xylose Isomerase and Xylulokinase in an Industrial Saccharomyces cerevisiae Strain - (Abstract)

Rich, J.O., Hughes, S.R., Bischoff, K.M., Leathers, T.D. 2008. Three-vector System for use on Automated Systems for High-level Functional Expression of Value-added Co-products with Xylose Isomerase and Xylulokinase in an Industrial Saccharomyces cerevisiae Strain [abstract]. Indiana Council of Engineering Societies. Abstract 13. p. 4.

Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system - (Peer Reviewed Journal)

Hughes, S.R., Sterner, D.E., Bischoff, K.M., Hector, R.E., Dowd, P.F., Qureshi, N., Bang, S.S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Dien, B.S., Mertens, J.A., Caughey, R.J., Liu, S., Butt, T.R., Labaer, J., Cotta, M.A., Rich, J.O. 2008. Engineered Saccharomyces cerevisiae Strain for Improved Xylose Utilization with a Three-plasmid SUMO Yeast Expression System. Plasmid Journal. 61:22-38.

Improving Biocatalysts for the Production of Biofuels from Lignocellulosic Feedstocks - (Abstract)

Rich, J.O., Hughes, S.R., Bischoff, K.M., Leathers, T.D., Liu, S. 2008. Improving Biocatalysts for the Production of Biofuels from Lignocellulosic Feedstocks [abstract]. U.S. Japan Natural Resources Meeting. p. 54-55.

Cellulosic Ethanol Production from Saccharomyces cerevisiae Engineered for Anaerobic Conversion of Pretreated Lignocellulosic Sugars to Ethanol - (Abstract)

Rich, J.O., Bischoff, K.M., Hughes, S.R. 2008. Cellulosic Ethanol Production from Saccharomyces cerevisiae Engineered for Anaerobic Conversion of Pretreated Lignocellulosic Sugars to Ethanol [abstract]. American Chemical Society. Paper ID1211547.

Saccharomyces cerevisiae mass transformed with FLEXGenes results in strain capable of anaerobic fermentation of pentose and hexose sugars - (Abstract)

Hughes, S.R., Javers, J., Labaer, J., Butt, T., Hector, R.E., Dien, B.S., Saha, B.C., Bischoff, K.M., Kohl, S., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae Mass Transformed with FLEXGenes Results in Strain Capable of Anaerobic Fermentation of Pentose and Hexose Sugars [abstract]. Society of Industrial Microbiology. Session 34, S164, p. 106.

Expression of a Heterologous Xylose Transporter in a Saccharomyces cerevisiae Strain Engineered to Utilize Xylose Improves Aerobic Xylose Co-consumption - (Peer Reviewed Journal)

Hector, R.E., Qureshi, N., Hughes, S.R., Cotta, M.A. 2008. Expression of a Heterologous Xylose Transporter in a Saccharomyces cerevisiae Strain Engineered to Utilize Xylose Improves Aerobic Xylose Consumption. Applied Microbiology and Biotechnology. 80(4):675-684.

Proteomic Approach for Molecular Mechanisms under Ethanol Stress in Lactobacillus buchneri - (Abstract)

Liu, S., Bischoff, K.M., Leathers, T.D., Hughes, S.R., Rich, J.O. 2008. Proteomic Approach for Molecular Mechanisms under Ethanol Stress in Lactobacillus buchneri [abstract]. American Society for Microbiology Meeting. 0-030. p. 155.

Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic sugars anaerobically to ethanol - (Abstract)

Hughes, S.R., Bischoff, K.M., Hector, R.E., Dien, B.S., Saha, B.C., Javers, J., Gibbons, W., Bang, S., Nelson, R., Standish, K., Cotta, M.A., Rich, J.O. 2008. Saccharomyces cerevisiae Engineered to Convert Pretreated Lignocellulosic Sugars Anaerobically to Ethanol [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit, Seminar 6. p. 1.

Three-vector system for high-level functional expression of value-added co-products with xylose isomerase and xylulokinase in an industrial saccharomyces cerevisiae strain - (Abstract)

Hughes, S.R., Jackson Jr, J.S., Dowd, P.F., Hector, R.E., Bischoff, K.M., Sterner, D., Bang, S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Li, X., Caughey, R.J., Liu, S., Skory, C.D., Butt, T., Labaer, J., Dien, B.S., Saha, B.C., Bowen, M., Qureshi, N., Rich, J.O., Leathers, T.D., Cotta, M.A., Farrelly, P., Slininger P.J. 2008. Three-vector System for High-level Functional Expression of Value-added Co-products with Xylose Isomerase and Xylulokinase in an Industrial Saccharomyces cerevisiae Strain [abstract]. Cambridge Healthtech Institute, Second Generation Biofuels Development Summit. Poster #1. p. 4.

Expression of a heterologous xylose transporter in a Saccharomyces cerevisiae strain engineered to utilize xylose increases xylose uptake and improves xylose/glucose co-consumption - (Abstract)

Hector, R.E., Qureshi, N., Hughes, S.R., Cotta, M.A. 2008. Expression of a heterologous xylose transporter in a Saccharomyces cerevisiae strain engineered to utilize xylose increases xylose uptake and improves xylose/glucose co-consumption [abstract]. In: Proceedings of the 30th Symposium on Biotechnology for Fuels and Chemicals, May 4-7, 2008, New Orleans, LA. p. 131.

Biofuels Group NSF DUSEL Project - (Abstract)

Bang, S., Sani, R., Bleakly, B., Bischoff, K.M., Hughes, S.R., Wagschal, K.C., Wong, D., Lee, C.C., Jordan, D.B., Pokkuluri, R., Schiffer, M. 2008. Biofuels Group NSF DUSEL Project [abstract]. Homestake DUSEL Spring Workshop. Talk 10. p. 2.

Ethanol production from modern biorefinery: Robotic platform for production of Saccharomyces cerevisiae engineered to convert pretreated lignocellulosic sugars to ethanol anaerobically - (Abstract)

Hughes, S.R., Rich, J.O. 2008. Ethanol Production from Modern Biorefinery: Robotic Platform for Production of Saccharomyces cerevisiae Engineered to Convert Pretreated Lignocellulosic Sugars to Ethanol Anaerobically [abstract]. Laboratory Robotics Information Group (LRIG). Seminar 1. p. 1.

Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain - (Peer Reviewed Journal)

Hughes, S.R., Dowd, P.F., Hector, R.E., Panavas, T., Sterner, D.E., Qureshi, N., Bischoff, K.M., Bang, S.B., Mertens, J.A., Johnson, E.T., Li, X., Jackson Jr, J.S., Caughey, R.J., Riedmuller, S.B., Bartolett, S., Liu, S., Rich, J.O., Farrelly, P.J., Butt, T.R., Labaer, J., Cotta, M.A. 2008. Lycotoxin-1 Insecticidal Peptide Optimized by Amino Acid Scanning Mutagenesis and Expressed as a Co-product in an Ethanologenix Saccharomyces cerevisiae Strain. Journal of Peptide Science. 14(9):1039-1050. Available: http://www3.interscience.wiley.com/cgi-bin/fulltext/119030240/PDFSTART.

Biodiesel production using heterogenous catalyst - (Abstract)

Tasaki, K., Moser, B.R., Doll, K.M., Hughes, S.R. 2008. Biodiesel production using heterogenous catalyst [abstract]. International Biofuels Development Summit. p. 2.

Genetically Modified Plants Containing Plant-Derived Genes for Broad Spectrum Insect Control to Reduce Mycotoxins: Bioactive Proteins - (Book/Chapter)

Dowd, P.F., Johnson, E.T., Pinkerton, T.S., Hughes, S.R. 2008. Genetically Modified Plants Containing Plant-Derived Genes for Broad Spectrum Insect Control to Reduce Mycotoxins. In: Wolf, T.V., Koch, T.J.P., editors. Bioactive Proteins. Hauppauge, NY, Nova Science Publishers. p. 127-150.

Regulatory concerns associated with use of value-added recombinant proteins and peptides screened in hgh-throughput for expression in fuel ethanol yeast strains - (Abstract)

Hughes, S.R., Dowd, P.F., Hector, R.E., Bischoff, K.M., Rich, J.O., Cotta, M.A., Slininger, P.J., Rosentrater, K.A. 2008. Regulatory concerns associated with use of value-added recombinant proteins and peptides screened in hgh-throughput for expression in fuel ethanol yeast strains [abstract]. LabAutomation 2008, Track 5, Seminar 4. p. 5.

Carbohydrate utilization and detection of a nucleotide hydrolase in Lactobacillus buchneri - (Abstract)

Liu, S., Bischoff, K.M., Price, N.P., Hughes, S.R. 2007. Carbohydrate utilization and detection of a nucleotide hydrolase in Lactobacillus buchneri [abstract]. 3rd International Symposium on Biocatalysis and Biotechnology. p. 49.

Regulatory steps associated with use of value-added recombinant proteins and peptides screened in high-throughput for expression in genetically engineered starch and cellulosic fuel ethanol yeast strains - (Abstract)

Hughes, S.R., Chen, H., Tsai, D., Thompson, W., Dowd, P.F., Hooberman, B., Benjamin, L., Alexander, L.J., Rosentrater, K.A., Rich, J.O. 2007. Regulatory steps associated with use of value-added recombinant proteins and peptides screened in high-throughput for expression in genetically engineered starch and cellulosic fuel ethanol yeast strains [abstract]. FDA Meeting. Talk 1.

Industrially useful microorganisms isolated from deep underground science and engineering laboratory (DUSEL) mine biofilms - (Abstract)

Hughes, S.R., Bang, S., Bleakley, B. 2007. Industrially useful microorganisms isolated from deep underground science and engineering laboratory (DUSEL) mine biofilms [abstract]. Meeting with South Dakota State Mine & Technology (SDSM&T) and South Dakota State University (SDSU). BioDUSEL Section, Day 2, Talk 3. p. 15.

Engineering Saccharomyces Cerevisiae for Ethanol Production from Agricultural Waste Products - (Abstract)

Hector, R.E., Qureshi, N., Hughes, S.R., Cotta, M.A. 2007. Engineering Saccharomyces cerevisiae for ethanol production from agricultural waste products [abstract]. Yeast Cell Biology. Paper No. 176.

Butanol Production from Wheat Straw by Simultaneous Saccharification and Fermentation Using Clostridium Beijerinckii: Part I-Batch Fermentation - (Peer Reviewed Journal)

Qureshi, N., Saha, B.C., Hector, R.E., Hughes, S.R., Cotta, M.A. 2008. Butanol production from wheat straw by simultaneous saccharification and fermentation using Clostridium beijerinckii: Part I - batch fermentation. Biomass and Bioenergy. 32:168-175.

Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis - (Peer Reviewed Journal)

Liu, S., Dien, B.S., Nichols, N.N., Bischoff, K.M., Hughes, S.R., Cotta, M.A. 2007. Coexpression of pyruvate decarboxylase and alcohol dehydrogenase genes in Lactobacillus brevis. FEMS Microbiolology Letters. 274(2):291-297.

Cloning and characterization of a recombinant family 5 endoglucanase from Bacillus licheniformis strain B-41361 - (Peer Reviewed Journal)

Bischoff, K.M., Liu, S., Hughes, S.R. 2007. Cloning and characterization of a recombinant family 5 endoglucanase from Bacillus licheniformis strain B-41361. Process Biochemistry. 42:1150-1154.

High-Throughput Fully Automated Construction of a Multiplex Library of Mutagenized Open Reading Frames for an Insecticidal Peptide Using a Plasmid-Based Functional Proteomic Robotic Workcell with Improved Vacuum System - (Peer Reviewed Journal)

Hughes, S.R., Dowd, P.F., Hector, R.E., Riedmuller, S.B., Bartolett, S., Mertens, J.A., Qureshi, N., Liu, S., Bischoff, K.M., Li, X., Jackson Jr, J.S., Sterner, D., Panavas, T., Cotta, M.A., Farrelly, P.J., Butt, T. 2007. High-throughput fully automated construction of a multiplex library of mutagenized open reading frames for an insecticidal peptide using a plasmid-based functional proteomic robotic workcell with improved vacuum system. Journal of Laboratory Automation. 12(4):202-212.

Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose Expressing "Stealth" Insecticidal Peptides. - (Abstract)

Hughes, S.R., Li, X., Hector, R.E., Bischoff, K.M., Cotta, M.A., Mertens, J.A., Dowd, P.F., Johnson, E.T. 2007. Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose Expressing "Stealth" Insecticidal Peptides [abstract]. ACS National Meeting. p. 108.

Recent Advances in Bioconversion of Agricultural Biomass to Butanol by Fermentation: Employing Potential of Available Renewable Resources to Produce a Superior Biofuel - (Abstract)

Qureshi, N., Saha, B.C., Li, X., Hughes, S.R., Cotta, M.A. 2007. Recent advances in bioconversion of agricultural biomass to butanol by fermentation: employing potential of available renewable resources to produce a superior biofuel [abstract]. American Chemical Society. Paper No. 178.

Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Improved Growth on Xylose Expressing Xylanase A(Xyn A) and Celulase F(Cel F) from Anaerobic Fungi Orpinomyces - (Abstract)

Hughes, S.R., Mertens, J.A., Qureshi, N., Cotta, M.A., Bischoff, K.M., Liu, S., Li, X. 2007. Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Improved Growth on Xylose Expressing Xylanase A(Xyn A) and Celulase F(Cel F) from Anaerobic Fungi Orpinomyces [abstract]. 2007 World Congress on Industrial Biotechnology and Bioprocessing. Seminar 2. p. 19.

Production of Highly Active Lignocellulose-Degrading Enzymes of Anaerobic Fungi by Industrially Relevant Fungi - (Abstract)

Li, X., Skory, C.D., Ximenes, E.A., Jordan, D.B., Dien, B.S., Hughes, S.R., Cotta, M.A. 2007. Production of highly active lignocellulose-degrading enzymes of anaerobic fungi by industrially relevant fungi [abstract].

Fully Automated Molecular Biology Routines on a Plasmid-Based Functional Proteomic Workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose and Engineered to Express an Insecticidal Peptide - (Abstract)

Hughes, S.R., Li, X., Liu, S., Bischoff, K.M., Mertens, J.A., Qureshi, N., Riedmuller, S., Bartolett, S., Farrelly, P., Panavas, T., Butt, T., Cotta, M.A. 2007. Fully Automated Molecular Biology Routines on a Plasmid-Based Functional Proteomic Workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose and Engineered to Express an Insecticidal Peptide [abstract]. PEPTALK 2007, Cambridge Healthtech Institute. Talk 83, p. 7.

Fully automated molecular biology: Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Optimized "Trojan Horse" Amino Acid Scanning Mutational Inserts. - (Abstract)

Hughes, S.R., Qureshi, N., Mertens, J.A., Cotta, M.A., Hector, R.E., Bischoff, K.M., Liu, S., Li, X-L., 2007. Fully automated molecular biology: Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Optimized "Trojan Horse" Amino Acid Scanning Mutational Inserts [abstract]. PEPTALK 2007. Cambridge Healthtech Institute. Poster 509, p. 4.

Adaptation to an automated platform of algorithmic combinations of advantageous mutations in genes generated using amino acid scanning mutational strategy. - (Abstract)

Riedmuller, S., Hughes, S.R., Hector, R.E., Bischoff, K.M., Farrelly, P., Cotta, M.A., Li, X. 2007. Adaptation to an automated platform of algorithmic combinations of advantageous mutations in genes generated using amino acid scanning mutational strategy [abstract]. PEPTALK 2007. Cambridge Healthtech Institute. Seminar 43. p. 4.

Expression of An at-Rich Xylanase Gene from the Anaerobic Fungus Orpinomyces Sp. Strain Pc-2 in and Secretion of the Heterologous Enzyme by Hypocrea Jecorina - (Peer Reviewed Journal)

Li, X., Skory, C.D., Ximenes, E.A., Jordan, D.B., Dien, B.S., Hughes, S.R., Cotta, M.A. 2007. Expression of an AT-rich xylanase gene from the anaerobic fungus Orpinomyces sp. strain PC-2 in and secretion of the heterologous enzyme by Hypocrea jecorina. Applied Microbiology and Biotechnology. 74:1264-1275.

Purification and Characterization of a Family 5 Endoglucanase from a Moderately Thermophilic Strain of Bacillus Licheniformis - (Peer Reviewed Journal)

Bischoff, K.M., Rooney, A.P., Li, X., Liu, S., Hughes, S.R. 2006. Purification and characterization of a family 5 endoglucanase from a moderately thermophilic strain of Bacillus licheniformis. Biotechnology Letters. 28:1761-1765.

Isolation of Xylose-Utilizing Thermophilic Bacteria from Dairy Manure Compost - (Abstract)

Bischoff, K.M., Liu, S., Edrington, T.S., Hughes, S.R. 2006. Isolation of xylose-utilizing thermophilic bacteria from dairy manure compost [abstract]. Extremophiles 2006. p. 19. Poster P045.

Production of Acetone Butanol (Ab) from Agricultural Residues Using Clostridium Acetobutylicum in Batch Reactors Coupled with Product Recovery - (Abstract)

Qureshi, N., Saha, B.C., Hughes, S.R., Cotta, M.A. 2006. Production of acetone butanol (AB) from agricultural residues using Clostridium acetobutylicum in batch reactors coupled with product recovery [abstract]. Proceedings of the Ninth International Workshop on the Regulation of Metabolism, Genetics, and Development of the Solvent and Acid Forming Clostridia. p. 29.

Functional Proteomic Plasmid-Based Integrated Workcell for High-Throughput Transformation of Bl21 De3 E. Coli for Expression in Vivo with Piromyces Strain Xylose Isomerase - (Abstract)

Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Li, X., Bischoff, K.M., Liu, S., Qureshi, N., Cotta, M.A., Skory, C.D., Gorsich, S.W., Farrelly, P.J. 2006. Functional proteomic plasmid-based integrated workcell for high-throughput transformation of BL21 DE3 E. coli for expression in vivo with piromyces strain xylose isomerase [abstract]. Midwest Laboratory Robotics Information Group. p. 2.

High-Throughput Screening of Cellulase F Mutants from Multiplexed Plasmid Sets Using An Automated Plate Assay on a Functional Proteomic Robotic Workcell - (Peer Reviewed Journal)

Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Li, X., Bischoff, K.M., Qureshi, N., Cotta, M.A., Farrelly, P.J. 2006. High-throughput screening of cellulase F mutants from multiplexed plasmid sets using an automated plate assay on a functional proteomic robotic workcell. Proteome Science. 4:10.

Codon Optimization of An A+t Rich Xylanase Gene from the Anaerobic Fungus Orpinomyces Pc-2 for Expression by Trichoderma Reesei - (Abstract)

Li, X., Jordan, D.B., Ximenes, E.A., Cotta, M.A., Dien, B.S., Hughes, S.R. 2006. Codon optimization of an A+T rich xylanase gene from the anaerobic fungus Orpinomyces PC-2 for expression by Trichoderma reesei [abstract]. In: Proceedings of the 9th International Workshop on Trichoderma and Gliocladium, April 6-8, 2006, Vienna, Austria. Paper No. T3.

Butanol Production from Corn Fiber Xylan Using Clostridium Acetobutylicum - (Peer Reviewed Journal)

Qureshi, N., Li, X., Hughes, S.R., Saha, B.C., Cotta, M.A. 2006. Butanol production from corn fiber xylan using Clostridium acetobutylicum. Biotechnology Progress. 22:673-680.

Automated Strategy Using a Functional Proteomic Assay to Identify and Isolate Cellulase F Mutants with Improved Activity from Multiplexed Sets of Plasmid - (Abstract)

Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Qureshi, N., Farrelly, P., Cotta, M.A. 2006. Automated strategy using a functional proteomic assay to identify and isolate cellulase F mutants with improved activity from multiplexed sets of plasmid [abstract]. PepTalk 2006. p. 10.

Mass Transformation of Plasmid Libraries of Cdna Or Mutagenized Clone Sets into Yeast Or Bacteria Using a Functional Proteomic Robotic Workcell - (Abstract)

Hughes, S.R., Riedmuller, S., Li, X., Qureshi, N., Liu, S., Bischoff, K.M., Cotta, M.A., Farrelly, P. 2006. Mass transformation of plasmid libraries of cDNA or mutagenized clone sets into yeast or bacteria using a functional proteomic robotic workcell [abstract]. PepTalk 2006. p. 10.

Plasmid-Based Functional Proteomic Robotic Workcell Process for High-Throughput Screening of Multiplexed Libraries of Mutagenized Clones - (Abstract)

Hughes, S.R., Mertens, J.A., Li, X., Bischoff, K.M. 2005. Plasmid-based functional proteomic robotic workcell process for high-throughput screening of multiplexed libraries of mutagenized clones [abstract]. Laboratory Robotics Information Group. p. 1.

Continuous Production of Ethanol in High Productivity Bioreactors Using Genetically Engineered Escherichia Coli Fbr5: Membrane and Fixed Cell Reactors - (Abstract)

Qureshi, N., Dien, B.S., Nichols, N.N., Liu, S., Hughes, S.R., Iten, L.B., Saha, B.C., Cotta, M.A. 2005. Continuous production of ethanol in high productivity bioreactors using genetically engineered Escherichia coli FBR5: membrane and fixed cell reactors [extended abstract]. American Institute of Chemical Engineers. Paper No. 589g.

Plasmid-Based Functional Proteomic Robotic Workcell Process for High-Throughput Screening of Multiplexed Libraries of Mutagenized Clones - (Abstract)

Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Qureshi, N., Bischoff, K.M., Jordan, D.B., Cotta, M.A., Farrelly, P. 2005. Plasmid-based functional proteomic robotic workcell process for high-throughput screening of multiplexed libraries of mutagenized clones [abstract]. Optimization high-throughput Cultures for Bioprocessing 2005. p. 3.

Functional Proteomic Workcell for High Volume Plasmid Preparations for Repeated in Vitro Protein Expression and High Throughput Screening to Identify Mutant Enyzmes for Use at Low Ph - (Abstract)

Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Jordan, D.B., Li, X., Qureshi, N., Cotta, M.A., Farrelly, P.J., Bischoff, K.M. 2005. Functional proteomic workcell for high volume plasmid preparations for repeated in vitro protein expression and high throughput screening to identify mutant enyzmes for use at low pH [abstract]. Optimization High-throughput Cultures for Bioprocessing 2005. 13:3.

Antimicrobial Susceptibility of Bacterial Contaminants from a Wet-Mill Ethanol Plant - (Abstract)

Bischoff, K.M., Skinner-Nemec, K., Leathers, T.D., Hughes, S.R. 2005. Antimicrobial susceptibility of bacterial contaminants from a wet-mill ethanol plant [abstract]. Society for Industrial Microbiology. Poster P42.

Characterization of Carboxymethylcellulase Activity from Geobacillus Stearothermophilus - (Abstract)

Bischoff, K.M., Li, X., Rooney, A.P., Liu, S., Hughes, S.R. 2005. Characterization of carboxymethylcellulase activity from Geobacillus stearothermophilus [abstract]. Society for Industrial Microbiology. Paper #P38.

Lactobacillus Brevis: a Potential Biocatalyst for Lignocellulosic Biomass to Ethanol - (Abstract)

Liu, S., Dien, B.S., Cotta, M.A., Bischoff, K.M., Hughes, S.R. 2005. Lactobacillus brevis: a potential biocatalyst for lignocellulosic biomass to ethanol [abstract]. Society of Industrial Microbiology. Paper #P05.

Development of a Liquid Handler Component for a Plasmid-Based Functional Proteomic Robotic Workcell - (Peer Reviewed Journal)

Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Bischoff, K.M., Cotta, M.A., Farrelly, P. 2005. Development of a liquid handler component for a plasmid-based functional proteomic robotic workcell. Journal of the Association for Laboratory Automation. 10(5):287-300.

Development of a Liquid Handler Component for a Functional Plasmid-Based Proteomic Workcell That Generates Multiplex Samples Expresses in Yeast - (Abstract)

Hughes, S.R., Riedmuller, S.B., Bischoff, K.M., Mertens, J.A., Li, X., Cotta, M.A., Farrelly, P.J. 2005. Development of a liquid handler component for a functional plasmid-based proteomic workcell that generates multiplex samples expresses in yeast [abstract]. Association for Laboratory Automation, LabAutomation 2005. Poster WP128.

Energy Efficient Recovery of Butanol from Model Solutions and Fermentation Broth by Adsorption - (Peer Reviewed Journal)

Qureshi, N., Hughes, S.R., Maddox, I.S., Cotta, M.A. 2005. Energy efficient recovery of butanol from model solutions and fermentation broth by adsorption. Bioprocess and Biosystems Engineering. 27(4):215-222.

A Plasmid-Based Functional Proteomic Workcell Used to Optimize Yeast for Fuel Ethanol Production - (Abstract)

Hughes, S.R., Li, X., Cotta, M.A., Mertens, J.A., Bischoff, K.M., Riedmuller, S., Farrelly, P., Patel, M., Brown, L., Carter, L. 2005. A plasmid-based functional proteomic workcell used to optimize yeast for fuel ethanol production [abstract]. CHI's Annual PEP Talk Meeting, January 10-14, 2005, San Diego, California. p. 3.

Modified Liquid Handler Used to Produce High Throughput High Quality Plasmids for Integration into Plasmid-Based Functional Proteomics Workcell - (Abstract)

Hughes, S.R., Li, X., Cotta, M.A., Mertens, J.A., Bischoff, K.M., Riedmuller, S., Farrelly, P., Patel, M., Brown, L., Carter, L. 2005. Modified liquid handler used to produce high throughput high quality plasmids for integration into plasmid-based functional proteomics workcell [abstract]. CHI's Annual PEP Talk Meeting, January 10-14, 2005, San Diego, California. Poster 151.

A Plasmid-Based Functional Proteomic Workcell Assay Designed to Screen Orpinomyces Cellulase F Nny Mutagenized Libraries in High Throughtput - (Abstract)

Hughes, S.R., Li, X., Cotta, M.A., Bischoff, K.M., Riedmuller, S., Farrelly, P. 2004. A plasmid-based functional proteomic workcell assay designed to screen Orpinomyces cellulase F NNY mutagenized libraries in high throughtput [abstract]. 36th Great Lakes Regional American Chemical Society Symposium, October 17-20, 2004, Peoria, Illinois. Poster 361.

A Plasmid-Based Functional Proteomic Workcell Used to Optimize Open Reading Frames and Generate Improved Strains for Commercial Use - (Abstract)

Hughes, S.R., Li, X., Cotta, M.A., Bischoff, K.M., Riedmuller, S., Farrelly, P. 2004. A plasmid-based functional proteomic workcell used to optimize open reading frames and generate improved strains for commercial use [abstract]. 36th Great Lakes Regional American Chemical Society Symposium, October 17-20, 2004, Peoria, Illinois. Poster 361.