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United States Department of Agriculture

Agricultural Research Service

JEFFREY A MERTENS
Bioenergy Research Unit
Research Molecular Biologist

Phone: (309) 681-6086
Fax: (309) 681-6427
Room 2127A
1815 N UNIVERSITY ST
PEORIA, IL, 61604

Projects
ADVANCED CONVERSION TECHNOLOGIES FOR SUGARS AND BIOFUELS: SUPERIOR FEEDSTOCKS, PRETREATMENTS, INHIBITOR REMOVAL, AND ENZYMES
Appropriated (D)
  Accession Number: 417041
PROCESS TECHNOLOGIES FOR PRODUCING BIOFUELS AND COPRODUCTS FROM LIGNOCELLULOSIC FEEDSTOCKS
Appropriated (D)
  Accession Number: 418680

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Kinetic properties of two Rhizopus exo-polygalacturonase enzymes hydrolyzing galacturonic acid oligomers using isothermal titration calorimetry - (Peer Reviewed Journal)
Mertens, J.A. 2013. Kinetic properties of two Rhizopus exo-polygalacturonase enzymes hydrolyzing galacturonic acid oligomers using isothermal titration calorimetry. Applied Biochemistry and Biotechnology. 170(8):2009-2020.
Growth and fermentation of D-xylose by Saccharomyces cerevisiae expressing a novel D-xylose isomerase originating from the bacterium Prevotella ruminicola TC2-24 - (Peer Reviewed Journal)
Hector, R.E., Dien, B.S., Cotta, M.A., Mertens, J.A. 2013. Growth and fermentation of D-xylose by Saccharomyces cerevisiae expressing a novel D-xylose isomerase originating from the bacterium Prevotella ruminicola TC2-24. Biotechnology for Biofuels. 6:84.
Investigation of accessory hemicellulases and pectinases for polysaccharide hydrolysis of ionic liquid pretreated biomass - (Abstract Only)
Barr, C.J., Mertens, J.A., Schall, C. 2012. Investigation of accessory hemicellulases and pectinases for polysaccharide hydrolysis of ionic liquid pretreated biomass [abstract]. American Institute of Chemical Engineers (AIChE).
The ultrasound-enhanced bioscouring performance of four polygalacturonase enzymes obtained from rhizopus oryzae - (Peer Reviewed Journal)
Easson, M.W., Mertens, J.A., Fortier, C.A., Slopek, R.P., Condon, B.D., Boykin, D.L., Tarver, M.R. 2013. The ultrasound-enhanced bioscouring performance of four polygalacturonase enzymes obtained from rhizopus oryzae. American Association of Textile Chemists and Colorists Review. 13(2):51-56.
Plant cell walls to ethanol. - (Peer Reviewed Journal)
Jordan, D.B., Bowman, M.J., Braker, J.D., Dien, B.S., Hector, R.E., Lee, C.C., Mertens, J.A., Wagschal, K.C. 2012. Plant cell walls to ethanol. Biochemical Journal. 442:247-252.
Critical cellulase and hemicellulase activities for hydrolysis of ionic liquid pretreated biomass - (Peer Reviewed Journal)
Barr, C.J., Mertens, J.A., Schall, C.A. 2012. Critical cellulase and hemicellulase activities for hydrolysis of ionic liquid pretreated biomass. Bioresource Technology. 104:480-485.
Subsite binding energies of an exo-polygalacturonase using isothermal titration calorimetry - (Peer Reviewed Journal)
Mertens, J.A., Hector, R.E., Bowman, M.J. 2012. Subsite binding energies of an exo-polygalacturonase using isothermal titration calorimetry. Thermochimica Acta. 527:219-222.
Biochemical processing of reed canarygrass into fuel ethanol - (Peer Reviewed Journal)
Dien, B.S., Casler, M.D., Hector, R.E., Iten, L.B., Nichols, N.N., Mertens, J.A., Cotta, M.A. 2012. Biochemical processing of reed canarygrass into fuel ethanol. International Journal of Low-Carbon Technologies. 7:338-347.
The bioscouring performance of four polygalacturonase enzymes - (Proceedings)
Easson, M.W., Mertens, J.A., Boykin, D.L., Fortier, C.A., Tarver, M.R., Condon, B.D. 2012. The bioscouring performance of four polygalacturonase enzymes. Proceedings of The American Association Of Textile Chemists And Colorists. 54-60.
Saccharomyces cerevisiae engineered for xylose metabolism requires gluconeogenesis and the oxidative branch of the pentose phosphate pathway for aerobic xylose assimilation Reprint Icon - (Peer Reviewed Journal)
Hector, R.E., Mertens, J.A., Bowman, M.J., Nichols, N.N., Cotta, M.A., Hughes, S.R. 2011. Saccharomyces cerevisiae engineered for xylose metabolism requires gluconeogenesis and the oxidative branch of the pentose phosphate pathway for aerobic xylose assimilation. Yeast. 28:645-660.
Deconstruction of ionic liquid pretreated lignocellulosic biomass using mono-component cellulases and hemicellulases and commercial mixtures - (Abstract Only) - (05-May-11)
Expression and characterization of fifteen Rhizopus oryzae 99-880 polygalacturonase enzymes in Pichia pastoris - (Peer Reviewed Journal)
Mertens, J.A., Bowman, M.J. 2011. Expression and characterization of fifteen Rhizopus oryzae 99-880 polygalacturonase enzymes in Pichia pastoris. Current Microbiology. 62(4):1173-1178.
Enzyme Systems for Optimization of Ionic-Liquid Pretreated Biomass Saccharification - (Abstract Only)
Barr, C.J., Wissinger, J., Samayam, I.P., Schall, C.A., Mertens, J.A. 2010. Enzyme Systems for Optimization of Ionic-Liquid Pretreated Biomass Saccharification [abstract]. American Institute of Chemical Engineers. p. 265.
Catalytic properties of two Rhizopus oryzae 99-880 glucoamylase enzymes without starch binding domains expressed in Pichia pastoris - (Peer Reviewed Journal)
Mertens, J.A., Braker, J.D., Jordan, D.B. 2010. Catalytic properties of two Rhizopus oryzae 99-880 glucoamylase enzymes without starch binding domains expressed in Pichia pastoris. Applied Biochemistry and Biotechnology. 162(8):2197-2213.
Enzyme Characterization of Cellulase and Hemicellulases Component Enzymes and Saccharification of Ionic Liquid Pretreated Lignocellulosic Biomass - (Abstract Only)
Barr, C.J., Schall, C.A., Mertens, J.A. 2010. Enzyme Characterization of Cellulase and Hemicellulases Component Enzymes and Saccharification of Ionic Liquid Pretreated Lignocellulosic Biomass [abstract]. Biotechnology for Fuels and Chemicals. p. 85.
Identification, Characterization, and Evolution of a Large Polygalacturonase Gene Family from Rhizopus oryzae - (Abstract Only) - (31-Jul-09)
Inhibition of Rhizopus Lactate Dehydrogenase by Fructose 1,6-bisphosphate - (Peer Reviewed Journal)
Skory, C.D., Mertens, J.A., Rich, J.O. 2009. Inhibition of Rhizopus lactate dehydrogenase by fructose 1,6-bisphosphate. Enzyme and Microbial Technology. 44(4):242-247.
Identification, Biochemical Characterization, and Evolution of the Rhizopus oryzae 99-880 Polygalacturonase Gene Family - (Peer Reviewed Journal)
Mertens, J.A., Burdick, R.C., Rooney, A.P. 2008. Identification, Biochemical Characterization, and Evolution of the Rhizopus oryzae 99-880 Polygalacturonase Gene Family. Fungal Genetics and Biology. 45(12):1616-1624.
Aminoalcohols as Probes of the Two-subsite Active Site of Beta-D-xylosidase from Selenomonas ruminantium - (Peer Reviewed Journal)
Jordan, D.B., Mertens, J.A., Braker, J.D. 2009. Aminoalcohols as Probes of the Two-subsite Active Site of Beta-D-xylosidase from Selenomonas ruminantium. Biochimica et Biophysica Acta. 1794(1):144-158.
Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system - (Peer Reviewed Journal)
Hughes, S.R., Sterner, D.E., Bischoff, K.M., Hector, R.E., Dowd, P.F., Qureshi, N., Bang, S.S., Grynavyski, N., Chakrabarty, T., Johnson, E.T., Dien, B.S., Mertens, J.A., Caughey, R.J., Liu, S., Butt, T.R., Labaer, J., Cotta, M.A., Rich, J.O. 2009. Engineered Saccharomyces cerevisiae strain for improved xylose utilization with a three-plasmid SUMO yeast expression system. Plasmid Journal. 61(1):22-38.
Isolation and characterization of 17 different genes encoding putative endopolygalacturonase genes from Rhizopus oryzae - (Abstract Only)
Mertens, J.A., Burdick, R.C. 2008. Isolation and characterization of 17 different genes encoding putative endopolygalacturonase genes from Rhizopus oryzae [abstract]. Society of Industrial Microbiology. p. 116.
Identification and transcriptional profiling of Pseudomonas putida genes involved in furoic acid metabolism - (Peer Reviewed Journal)
Nichols, N.N., Mertens, J.A. 2008. Identification and transcriptional profiling of Pseudomonas putida genes involved in furoic acid metabolism. FEMS Microbiology Letters. 284:52-57.
Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain - (Peer Reviewed Journal)
Hughes, S.R., Dowd, P.F., Hector, R.E., Panavas, T., Sterner, D.E., Qureshi, N., Bischoff, K.M., Bang, S.B., Mertens, J.A., Johnson, E.T., Li, X., Jackson Jr, J.S., Caughey, R.J., Riedmuller, S.B., Bartolett, S., Liu, S., Rich, J.O., Farrelly, P.J., Butt, T.R., Labaer, J., Cotta, M.A. 2008. Lycotoxin-1 insecticidal peptide optimized by amino acid scanning mutagenesis and expressed as a co-product in an ethanologenix Saccharomyces cerevisiae strain. Journal of Peptide Science. 14(9):1039-1050. Available: http://www3.interscience.wiley.com/cgi-bin/fulltext/119030240/PDFSTART.
Molecular cloning and expression of three polygalacturonase cDNAs from the tarnished plant bug, Lygus lineolaris Reprint Icon - (Peer Reviewed Journal)
Allen, M.L., Mertens, J.A. 2008. Molecular cloning and expression of three polygalacturonase cDNAs from the tarnished plant bug, Lygus lineolaris. J. Insect Sci. 8 Article 27:1-14. 2008.
High-Throughput Fully Automated Construction of a Multiplex Library of Mutagenized Open Reading Frames for an Insecticidal Peptide Using a Plasmid-Based Functional Proteomic Robotic Workcell with Improved Vacuum System - (Peer Reviewed Journal)
Hughes, S.R., Dowd, P.F., Hector, R.E., Riedmuller, S.B., Bartolett, S., Mertens, J.A., Qureshi, N., Liu, S., Bischoff, K.M., Li, X., Jackson Jr, J.S., Sterner, D., Panavas, T., Cotta, M.A., Farrelly, P.J., Butt, T. 2007. High-throughput fully automated construction of a multiplex library of mutagenized open reading frames for an insecticidal peptide using a plasmid-based functional proteomic robotic workcell with improved vacuum system. Journal of Laboratory Automation. 12(4):202-212.
Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose Expressing "Stealth" Insecticidal Peptides. - (Abstract Only)
Hughes, S.R., Li, X., Hector, R.E., Bischoff, K.M., Cotta, M.A., Mertens, J.A., Dowd, P.F., Johnson, E.T. 2007. Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and characterization of yeast strains optimized for growth on xylose expressing "stealth" insecticidal peptides [abstract]. American Chemical Society. p. 108.
Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Improved Growth on Xylose Expressing Xylanase A(Xyn A) and Celulase F(Cel F) from Anaerobic Fungi Orpinomyces - (Abstract Only)
Hughes, S.R., Mertens, J.A., Qureshi, N., Cotta, M.A., Bischoff, K.M., Liu, S., Li, X. 2007. Plasmid-based functional proteomic workcell evaluation and characterization of yeast strains with improved growth on xylose expressing Xylanase A(Xyn A) and Celulase F(Cel F) from anaerobic fungi Orpinomyces [abstract]. World Congress on Industrial Biotechnology and Bioprocessing. Seminar 2. p. 19.
Isolation and Characterization of Two Genes That Encode Active Glucoamylase Without a Starch Binding Domain from a Type II Rhizopus Oryzae - (Peer Reviewed Journal)
Mertens, J.A., Skory, C.D. 2007. Isolation and characterization of two genes that encode active glucoamylase without a starch binding domain from Rhizopus oryzae. Current Microbiology. 54:462-466.
Fully automated molecular biology: Plasmid-Based Functional Proteomic Workcell Evaluation and Characterization of Yeast Strains with Optimized "Trojan Horse" Amino Acid Scanning Mutational Inserts. - (Abstract Only)
Hughes, S.R., Qureshi, N., Mertens, J.A., Cotta, M.A., Hector, R.E., Bischoff, K.M., Liu, S., Li, X-L., 2007. Fully automated molecular biology: Plasmid-based functional proteomic workcell evaluation and characterization of yeast strains with optimized "Trojan Horse" amino acid scanning mutational inserts [abstract]. PEPTALK 2007. Cambridge Healthtech Institute. Poster 509, p. 4.
Fully Automated Molecular Biology Routines on a Plasmid-Based Functional Proteomic Workcell: Evaluation and Characterization of Yeast Strains Optimized for Growth on Xylose and Engineered to Express an Insecticidal Peptide - (Abstract Only)
Hughes, S.R., Li, X., Liu, S., Bischoff, K.M., Mertens, J.A., Qureshi, N., Riedmuller, S., Bartolett, S., Farrelly, P., Panavas, T., Butt, T., Cotta, M.A. 2007. Fully automated molecular biology routines on a plasmid-based functional proteomic workcell: Evaluation and characterization of yeast strains optimized for growth on xylose and engineered to express an insecticidal peptide [abstract]. PEPTALK 2007, Cambridge Healthtech Institute. Talk 83, p. 7.
Isolation and Characterization of a Second Glucoamylase Without a Starch Binding Domain from Rhizopus Oryzae - (Peer Reviewed Journal)
Mertens, J.A., Skory, C.D. 2007. Isolation and characterization of a second glucoamylase gene without a starch binding domain from Rhizopus oryzae. Enzyme and Microbial Technology. 40:874-880.
Identification and Transcriptional Profiling of Furoic Acid Metabolism Genes in Pseudomonas Putida - (Abstract Only)
Nichols, N.N., Mertens, J.A., Dien, B.S. 2006. Identification and transcriptional profiling of furoic acid metabolism genes in Pseudomonas putida [abstract]. American Society for Microbiology. Paper No. Q-449.
Plasmids for Expression of Heterologous Proteins in Rhizopus Oryzae - (Peer Reviewed Journal)
Mertens, J.A., Skory, C.D., Ibrahim, A.S. 2006. Plasmids for expression of heterologous proteins in Rhizopus oryzae. Archives of Microbiology. 186:41-50.
Functional Proteomic Plasmid-Based Integrated Workcell for High-Throughput Transformation of Bl21 De3 E. Coli for Expression in Vivo with Piromyces Strain Xylose Isomerase - (Abstract Only)
Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Li, X., Bischoff, K.M., Liu, S., Qureshi, N., Cotta, M.A., Skory, C.D., Gorsich, S.W., Farrelly, P.J. 2006. Functional proteomic plasmid-based integrated workcell for high-throughput transformation of BL21 DE3 E. coli for expression in vivo with piromyces strain xylose isomerase [abstract]. Midwest Laboratory Robotics Information Group. p. 2.
High-Throughput Screening of Cellulase F Mutants from Multiplexed Plasmid Sets Using An Automated Plate Assay on a Functional Proteomic Robotic Workcell - (Peer Reviewed Journal)
Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Li, X., Bischoff, K.M., Qureshi, N., Cotta, M.A., Farrelly, P.J. 2006. High-throughput screening of cellulase F mutants from multiplexed plasmid sets using an automated plate assay on a functional proteomic robotic workcell. Proteome Science. 4:10.
Automated Strategy Using a Functional Proteomic Assay to Identify and Isolate Cellulase F Mutants with Improved Activity from Multiplexed Sets of Plasmid - (Abstract Only)
Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Qureshi, N., Farrelly, P., Cotta, M.A. 2006. Automated strategy using a functional proteomic assay to identify and isolate cellulase F mutants with improved activity from multiplexed sets of plasmid [abstract]. PepTalk 2006. p. 10.
Plasmid-Based Functional Proteomic Robotic Workcell Process for High-Throughput Screening of Multiplexed Libraries of Mutagenized Clones - (Abstract Only)
Hughes, S.R., Mertens, J.A., Li, X., Bischoff, K.M. 2005. Plasmid-based functional proteomic robotic workcell process for high-throughput screening of multiplexed libraries of mutagenized clones [abstract]. Laboratory Robotics Information Group. p. 1.
Plasmid-Based Functional Proteomic Robotic Workcell Process for High-Throughput Screening of Multiplexed Libraries of Mutagenized Clones - (Abstract Only)
Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Qureshi, N., Bischoff, K.M., Jordan, D.B., Cotta, M.A., Farrelly, P. 2005. Plasmid-based functional proteomic robotic workcell process for high-throughput screening of multiplexed libraries of mutagenized clones [abstract]. Optimization high-throughput Cultures for Bioprocessing 2005. p. 3.
Functional Proteomic Workcell for High Volume Plasmid Preparations for Repeated in Vitro Protein Expression and High Throughput Screening to Identify Mutant Enyzmes for Use at Low Ph - (Abstract Only)
Hughes, S.R., Riedmuller, S.B., Mertens, J.A., Jordan, D.B., Li, X., Qureshi, N., Cotta, M.A., Farrelly, P.J., Bischoff, K.M. 2005. Functional proteomic workcell for high volume plasmid preparations for repeated in vitro protein expression and high throughput screening to identify mutant enyzmes for use at low pH [abstract]. Optimization High-throughput Cultures for Bioprocessing 2005. 13:3.
Development of Plasmids for Expression of Heterologous Proteins in Rhizopus Oryzae - (Abstract Only)
Mertens, J.A., Skory, C.D. 2005. Development of plasmids for expression of heterologous proteins in Rhizopus oryzae [abstract]. Society of Industrial Microbiology. p. 100.
Development of a Liquid Handler Component for a Plasmid-Based Functional Proteomic Robotic Workcell - (Peer Reviewed Journal)
Hughes, S.R., Riedmuller, S., Mertens, J.A., Li, X., Bischoff, K.M., Cotta, M.A., Farrelly, P. 2005. Development of a liquid handler component for a plasmid-based functional proteomic robotic workcell. Journal of the Association for Laboratory Automation. 10(5):287-300.
Development of a Liquid Handler Component for a Functional Plasmid-Based Proteomic Workcell That Generates Multiplex Samples Expresses in Yeast - (Abstract Only)
Hughes, S.R., Riedmuller, S.B., Bischoff, K.M., Mertens, J.A., Li, X., Cotta, M.A., Farrelly, P.J. 2005. Development of a liquid handler component for a functional plasmid-based proteomic workcell that generates multiplex samples expresses in yeast [abstract]. Association for Laboratory Automation, LabAutomation 2005. Poster WP128.
A Plasmid-Based Functional Proteomic Workcell Used to Optimize Yeast for Fuel Ethanol Production - (Abstract Only)
Hughes, S.R., Li, X., Cotta, M.A., Mertens, J.A., Bischoff, K.M., Riedmuller, S., Farrelly, P., Patel, M., Brown, L., Carter, L. 2005. A plasmid-based functional proteomic workcell used to optimize yeast for fuel ethanol production [abstract]. CHI's Annual PEP Talk Meeting, January 10-14, 2005, San Diego, California. p. 3.
Modified Liquid Handler Used to Produce High Throughput High Quality Plasmids for Integration into Plasmid-Based Functional Proteomics Workcell - (Abstract Only)
Hughes, S.R., Li, X., Cotta, M.A., Mertens, J.A., Bischoff, K.M., Riedmuller, S., Farrelly, P., Patel, M., Brown, L., Carter, L. 2005. Modified liquid handler used to produce high throughput high quality plasmids for integration into plasmid-based functional proteomics workcell [abstract]. CHI's Annual PEP Talk Meeting, January 10-14, 2005, San Diego, California. Poster 151.
Discovery and Characterization of a Second Glucoamylase Gene in Rhizopus Oryzae - (Abstract Only)
Mertens, J.A., Skory, C.D. 2004. Discovery and characterization of a second glucoamylase gene in rhizopus oryzae [abstract]. Society of Industrial Microbiology. p. 96.
Last Modified: 7/25/2014
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