Projects

Genomic Approaches to Improving Transport and Detoxification of Selected Mineral Elements in Crop Plants Appropriated (D)   Accession Number: 410401

Genomics Approaches for Improving Nutritional Quality of Food Crop Species Appropriated (D)   Accession Number: 410826

Characterization of the Tomato Extracellular Proteome, Or Secretome, Using Integrated Functional and Computational Strategies Nonfunded Cooperative Agreement (N)   Accession Number: 411771

Characterization of the Tomato Extracellular Proteome, Or Secretome, Using Integrated Functinal and Computational Strategies Nonfunded Cooperative Agreement (N)   Accession Number: 412736

Control of Carotenoid Biosynthesis by a Novel Regulatory Gene Identified in Cauliflower Reimbursable (R)   Accession Number: 411115

Publications

Involvement of a Broccoli COQ5 Methyltransferase in the Production of Volatile Selenium Compounds - (Peer Reviewed Journal)

Li, L., Zhou, X., Yuan, Y., Yang, Y., Rutzke, M., Thannhauser, T.W., Kochian, L.V. 2009. Involvement of a Broccoli COQ5 Methyltransferase in the Production of Volatile Selenium Compounds. Plant Physiology. 151:528-540.

Proteomic analysis of B-aminobutyric acid priming and aba-induction of drought resistance in crabapple (Malus pumila): effect on general metabolism, the phenylpropanoid pathway and cell wall enzymes - (Peer Reviewed Journal)

Macarisin, D., Wisniewski, M.E., Bassett, C.L., Thannhauser, T.W. 2009. Proteomic analysis of B-aminobutyric acid priming and aba-induction of drought resistance in crabapple (Malus pumila): effect on general metabolism, the phenylpropanoid pathway and cell wall enzymes. Plant Cell and Environment. doi: 10.1111/j.1365-3040. 2009.02025.x

A broccoli COQ5 methyltransferase involved in ubiquinone biosynthesis mediates selenium volatilization - (Abstract)

Li, L., Zhou, X., Yang, Y., Thannhauser, T.W., Kochian, L.V. 2009. A broccoli COQ5 methyltransferase involved in ubiquinone biosynthesis mediates selenium volatilization. Meeting Abstract.

Protein N- and C-Termini Identification Using Mass Spectrometry and Isotopic Labeling - (Peer Reviewed Journal)

Xiang, B., Yang, X., Thannhauser, T.W. 2009. Protein N- and C-Termini Identification Using Mass Spectrometry and Isotopic Labeling. Journal of Rapid Communications in Mass Spectroscopy. 23:2102-2106.

A Comparison of Protein Extraction Methods Suitable for Gel-Based Proteomics Studies of Aphid Proteins - (Peer Reviewed Journal)

Cilia, M., Fish, T., Yang, X., Mclaughlin, M., Thannhauser, T.W., Gray, S.M. 2009. A Comparison of Protein Extraction Methods Suitable for Gel-Based Proteomics Studies of Aphid Proteins. Journal of Biomolecular Techniques. 20(4):201-215.

Salt Induced and Salt Suppressed Proteins in Tomato Leaves - (Peer Reviewed Journal)

Suping, Z., Zhou, S., Fish, T., Thannhauser, T.W. 2009. Salt Induced and Salt Suppressed Proteins in Tomato Leaves. Journal of the American Society for Horticultural Science. 134(2):289-294.

Drosophila ABC Transporter DmHMT-1 Confers Tolerance to Cadmium. - (Peer Reviewed Journal)

Sooksa-Nguan, T., Yakubov, B., Kozlovskyy, V.I., Barcume, C.M., Thannhauser, T.W., Rutzke, M.A., Hart, J.J., Kochian, L.V., Vatamaniuk, O.K. 2009. Drosophila ABC Transporter DmHMT-1 Confers Tolerance to Cadmium. Journal of Biological Chemistry. 284:354-362.

Effects of Ascorbic Acid, Phytic Acid and Tannic Acid on Iron Bioavailability from Reconstituted Ferritin Measured by an In Vitro Digestion/Caco-2 Cell Model - (Peer Reviewed Journal)

Jin, F., Frohman, C., Glahn, R.P., Thannhauser, T.W., Welch, R.M. 2009. Effects of Ascorbic Acid, Phytic Acid and Tannic Acid on Iron Bioavailability from Reconstituted Ferritin Measured by an In Vitro Digestion/Caco-2 Cell Model. British Journal of Nutrition. 101(7):312-316.

An evaluation of the basis and consequences of a stay-green mutation in the navel negra (nan) citrus mutant using transcriptomic and proteomic profiling and metabolite analysis - (Peer Reviewed Journal)

Alos, E., Roca, M., Iglesias, D., Minguez-Mosquera, M., Borges-Damasceno, C., Thannhauser, T.W., Campbell-Rose, J., Talon, M., Cercos, M. 2008. An evaluation of the basis and consequences of a stay-green mutation in the navel negra (nan) citrus mutant using transcriptomic and proteomic profiling and metabolite analysis. Journal of Plant Physiology. 147:1300-1315.

Role of Copper,zinc-Superoxide Dismutase in Catalyzing Nitrotyrosine Formation in Murine Liver - (Peer Reviewed Journal)

Zhu, J., Zhang, X., Roneker, C.A., Mcclung, J.P., Zhang, S., Thannhauser, T.W., Ripoll, D.R., Sun, Q., Lei, X. 2008. ROLE OF COPPER,ZINC-SUPEROXIDE DISMUTASE IN CATALYZING NITROTYROSINE FORMATION IN MURINE LIVER. Journal of Free Radical Biology and Medicine. 45:611-618.

Proteomic Analysis of B-Aminobutyric Acid-Primed Drought Resistance in Crabapple Seedlings - (Abstract)

Macarisin, D., Wisniewski, M.E., Bassett, C.L., Thannhauser, T.W. 2008. Proteomic analysis of b-aminobutyric acid-primed drought resistance in crabapple seedlings. Bio-Technology Fruit Symposium. Acta Hort. Book of Abstracts. 16:95.

Proteomic analysis of drought resistance in crabapple seedlings primed by the xenobiotic Beta-aminobutyric acid - (Abstract)

Macarisin, D., Wisniewski, M.E., Bassett, C.L., Thannhauser, T.W. 2008. Proteomic analysis of drought resistance in crabapple seedlings primed by the xenobiotic Beta-aminobutyric acid. Mid Atlantic Plant Molecular Biology Society Conference. Book of Abstracts Pg. 15-16.

Getting something for nothing: Regeneration of peptide signals from apparently exhausted MALDI samples by ¿waterboarding" - (Abstract)

Yang, X., Thannhauser, T.W. 2008. Getting something for nothing: Regeneration of peptide signals from apparently exhausted MALDI samples by ¿waterboarding". Journal of Biomolecular Techniques. Vol. 19:52.

Reproducibility of an Integrated Quantitation Method Coupling 2D GeLC-MS/MS with the emPAI for Comparative Proteomics - (Abstract)

Yang, Y., Zhang, S., Thannhauser, T.W. 2008. Reproducibility of an Integrated Quantitation Method Coupling 2D GeLC-MS/MS with the emPAI for Comparative Proteomics. Journal of Biomolecular Techniques. Vol. 19:52.

Characterization of Extracellular Proteins in Tomato Fruit using Lectin Affinity Chromatography and LC-MALDI-MS/MS analysis - (Abstract)

Hucko, S., Catala, C., Howe, K.J., Rose, J., Thannhauser, T.W. 2008. Characterization of Extracellular Proteins in Tomato Fruit using Lectin Affinity Chromatography and LC-MALDI-MS/MS analysis. Journal of Biomolecular Techniques. Vol. 19:57

Phytoene desaturase is present in a large protein complex in the plastid membrane - (Peer Reviewed Journal)

Lopez, A., Yang, Y., Thannhauser, T.W., Li, L. 2008. Phytoene desaturase is present in a large protein complex in the plastid membrane. Physiologia Plantarum. 133:190-198.

Coupling genetics and proteomics to identify aphid proteins associated with vector-specific transmission of Polerovirus (Luteoviridae) - (Peer Reviewed Journal)

Yang, X., Thannhauser, T.W., Burrows, M.E., Cox Foster, D., Gildow, F., Gray, S.M. 2008. Coupling genetics and proteomics to identify aphid proteins associated with vector-specific transmission of Polerovirus (Luteoviridae). Journal of Virology. 82:291-299.

A Comparison of nLC-ESI-MS/MS and nLC-MALDI-MS/MS for GeLC-based Protein Identification and iTRAQ-based Shotgun Quantitative Proteomics - (Peer Reviewed Journal)

Yang, Y., Howe, K.J., Zhang, S., Wilson, D., Moser, F., Irwin, D., Thannhauser, T.W. 2007. A comparison of nlc-esi-ms/ms and nlc-maldi-ms/ms for gelc-based protein identification and itraq-based shotgun quantitative proteomics. Journal of Biomolecular Techniques. 18:226-237.

On slide chemical modification as a means to improve confidence in protein identifications made by peptide mass finger prints - (Abstract)

Yang, X., Thannhauser, T.W. 2007. On slide chemical modification as a means to improve confidence in protein identifications made by peptide mass finger prints [abstract]. Journal of Biomolecular Techniques. 18:67.

A Comparison of nLC-ESI-MS/MS and nLC-MALDI-MS/MS for GeLC-based Protein Identification and iTRAQ-based Shotgun Quantitative Proteomics - (Abstract)

Yang, Y., Howe, K.J., Zhang, S., Thannhauser, T.W. 2007. A comparison of nlc-esi-ms/ms and nlc-maldi-ms/ms for gelc-based protein identification and itraq-based shotgun quantitative proteomics [abstract]. Journal of Biomolecular Techniques. 18:66.

Joint linkage-association analysis of aluminum tolerance in maize - (Abstract)

Hoekenga, O., Buckler Iv, E.S., Maron, L., Magalhaes, J., Kirst, M., Krill, A., Lyi, M., Rose, J., Thannhauser, T.W., Kochian, L.V. 2007. Joint linkage-association analysis of aluminum tolerance in maize [abstract]. Plant and Animal Genome VX Conference Abstracts. p. 55.

Development of an integrated approach for evaluation of 2-D gel image analysis: Impact of multiple proteins in single spots on comparative proteomics in conventional 2-D gel/MALDI workflow - (Peer Reviewed Journal)

Yang, Y., Thannhauser, T.W., Li, L., Zhang S. Development of an integrated approach for evaluation of 2D gel image analysis: Impact of multiple proteins in single spots on comparative proteomics in conventional 2-D gel/MALDI workflow. Electrophoresis 28:2080-2094.

High Throughput Protein Identification Using 2-Dimensional Difference Gel Electrophoresis and Robotic Spot Picking for Aluminum Tolerance-Related Maize Root Tip Proteins - (Abstract)

Thannhauser, T.W., Yang, Y., Zhang, S., Sherwood, R., Isaac, I. 2006. High throughput protein identification using 2-dimensional difference gel electrophoresis and robotic spot picking for aluminum tolerance-related maize root tip proteins. Journal of American Society for Mass Spectrometry. 17:46.

Comparison of Protein Identification by Maldi-Tof/tof and Lc Electrospray Ionization Tandem Ms for 2d Gel Separated Cauliflower Samples - (Abstract)

Thannhauser, T.W., Yang, Y., Zhang, S. 2006. Comparison of protein identification by maldi-tof/tof and lc electrospray ionization tandem ms for 2d gel separated cauliflower samples. Journal of Biomolecular Techniques. 17:48.

Itraq Based Protein Quantitative Analysis of the Cell Wall Proteome of Pathogen-Infected Tomato Leaves - (Abstract)

Thannhauser, T.W., Yang, Y., Zhang, S., Damasceno, C., Sherwood, R., Rose, J. 2006. Itraq based protein quantitative analysis of the cell wall proteome of pathogen-infected tomato leaves. Journal of Biomolecular Techniques. 17:40.

Proteins in the Aphid Schizaphis Graminum Associated with Vectoring Luteoviruses - (Abstract)

Thannhauser, T.W., Yang, X., Gray, S.M., Burrows, M.E., Cox-Foster, D., Gildow, F. 2006. Proteins in the aphid schizaphis graminum associated with vectoring luteoviruses. Phytopathology. 96:S127.

Interdisciplinary Analysis of Aluminum Tolerance in Maize - (Abstract)

Hoekenga, O., Buckler Iv, E.S., Maron, L., Magalhaes, J., Isaacson, T., Kirst, M., Krill, A., Lyi, S.M., Rose, J., Thannhauser, T.W., Kochian, L.V. 2006. Interdisciplinary analysis of aluminum tolerance in maize [abstract]. Plant and Animal Genome VX Conference Abstracts. p. 226.

Or, a Plastid Protein, Accounts for the High Level Accumulation of Beta-Carotene in Cauliflower - (Abstract)

Lu, S., O'Halloran, D.M., Cosman, K., Yang, Y., Xiang, B., Thannhauser, T.W., Li, L. 2005. Or, a plastid protein, accounts for the high level accumulation of beta-carotene in cauliflower. American Society of Plant Biologists Annual Meeting. p. 606.

Differential Protein Expression in Brassica Oleracia: Changes Associated with the Ability to Accumulate High Concentrations of B-Carotene - (Abstract)

Thannhauser, T.W., Yang, Y., Xiang, B., Lu, S., Li, L. 2005. Differential protein expression in brassica oleracia: changes associated with the ability to accumulate high concentrations of b-carotene [abstract]. Journal of Biomolecular Techniques. 16:60.