Projects

Development of Cold Storage Technology for Mass-Reared and Laboratory-Colonized Insects Appropriated (D)   Accession Number: 409603

Mass-Cryopreservation of Insect Embryos for Development of Back-Up Repositories for Production Facilities Reimbursable (R)   Accession Number: 414178

Characterization of Diapause, Cold Tolerance and Diversity in Pest and Beneficial Insects Using Physiological and Molecular Methods Specific Cooperative Agreement (S)   Accession Number: 409908

Evaluation of a Mass-Cryopreservation System for Insect Embryos Specific Cooperative Agreement (S)   Accession Number: 414373

Expression of Chilling Damage Through Maternal Inheritance in a Parasitic Wasp Specific Cooperative Agreement (S)   Accession Number: 418057

Publications

Quality Testing of Three Species of Tephritid Fruit Flies After Embryo Cryopreservation - (Peer Reviewed Journal) - (13-Nov-09)

A Template for Insect Cryopreservation - (Book/Chapter) - (17-Sep-09)

Cold Storage of Gonatocerus Ashmeadi Girault (Hymenoptera: Mymaridae): Extended Emergence, Parental and Progeny Fitness - (Proceedings/Symposium)

Leopold, R.A., Chen, W. 2008. Cold storage of Gonatocerus ashmeadi Girault (Hymenoptera: Mymaridae): Extended emergence, parental and progeny fitness. CDFA Pierce's Disease Control Program Research Symposium. p. 35-38.

Cold storage of adult Gonatocerus ashmeadi Girault (Mymaridae: Hymenoptera) and effects on maternal and progeny fitness - (Peer Reviewed Journal)

Chen, W., Leopold, R.A., Boetel, M.A. 2008. Cold storage of adult Gonatocerus ashmeadi Girault (Hymenoptera: Mymaridae) and effects on maternal and progeny fitness. Journal of Economic Entomology. 101(6):1760-1770.

Cold Storage Effects on Maternal and Progeny Quality of Gonatocerus ashmeadi Girault (Hymenoptera: Mymaridae) - (Peer Reviewed Journal)

Chen, W., Leopold, R.A., Harris, M.O. 2008. Cold Storage Effects on Maternal and Progeny Quality of Gonatocerus ashmeadi Girault (Hymenoptera: Mymaridae). Biological Control. 46(2):122-132.

Cold storage of the adult stage of Gonatocerus ashmeadi girault: the impact on maternal and progeny quality - (Abstract)

Leopold, R.A., Chen, W. 2007. Cold storage of the adult stage of Gonatocerus ashmeadi girault: the impact on maternal and progeny quality [abstract]. 2007 Pierce's Disease Research Symposium. p. 42.

The expression of chilling injury in the progeny of the parasitic wasp, Gonatocerus ashmeadi - (Abstract)

Leopold, R.A., Chen, W. 2007. The expression of chilling injury in the progeny of the parasitic wasp, Gonatocerus ashmeadi. J.C. van Lenteren, P. DeClercq & M. Johnson, 2007. Precedings of 11th meeting of the Working Group Arthropod Mass Rearing and Quality Control, 28 October - 1 November 2007, Montreal, Canada. Bulletin IOBC Global No. 3, p. 78-81.

Insect Cold Storage Technology: Design and Practical Applications - (Abstract)

Leopold, R.A. 2007. Insect Cold Storage Technology: Design and Practical Applications. Proceedings of International Congress of Insect Biotechnology and Industry. Entomological Research 37(Suppl. 1):A23.

The latent expression of chilling injury in the F1 progeny of the parasitic wasp, Gonatocerus ashmeadi - (Abstract)

Leopold, R.A., Chen, W. 2007. The latent expression of chilling injury in the F1 progeny of the parasitic wasp, Gonatocerus ashmeadi [abstract]. Cryobiology. 55(3):376-377.

Progeny quality of Gonatocerus ashmeadi Girault (Hymenoptera: Mymaridae)reared on stored eggs of Homalodisca coagulata (Say) (Homoptera: Cicadellidae) - (Peer Reviewed Journal)

Chen, W., Leopold, R.A. 2007. Progeny quality of Gonatocerus ashmeadi (Hymenoptera: Mymaridae)reared on stored eggs of Homalodisca coagulata (Homoptera: Cicadellidae). Journal of Economic Entomology. 100(3):685-694.

Cryopreservation of Mexican Fruit Fly by Vitrification: Stage Selection and Avoidance of Thermal Stress - (Peer Reviewed Journal)

Rajamohan, A., Leopold, R.A. 2007. Cryopreservation of Mexican fruit flies by vitrification: Stage selection and avoidance of thermal stress. Cryobiology. 54(1):44-54.

Colony Maintenance and Mass-Rearing: Using Cold Storage Technology for Extending the Shelf-Life of Insects - (Book/Chapter)

Leopold, R.A. 2007. Colony maintenance and mass-rearing: using cold storage technology for extending the shelf-life of insects. Book Chapter. In: Vreysen, M.J.B., Robinson, A.S., Hendrichs, J., Editors. Area-Wide Control of Insects: From Research to Field Implementation. The Netherlands: Springer, Dordrecht. p. 149-162.

Development and Reproduction of the Egg Parasitoid, Gonatocerus Ashmeadi Girault (Hymenoptera: Mymaridae) As a Function of Temperature - (Peer Reviewed Journal)

Chen, W., Leopold, R.A., Morgan, D.J., Harris, M.O. 2006. Development and reproduction of the egg parasitoid, Gonatocerus ashmeadi Girault (Hymenoptera: Mymaridae) as a function of temperature. Environmental Entomology. 35(5):1178-1187.

Ultrastructural Contributions to the Study of the Glassy-Winged Sharpshooter and Pierce's Disease - (Proceedings/Symposium)

Freeman, T.P., Leopold, R.A., Nelson, D.R., Buckner, J.S., Henneberry, T.J. 2004. Ultrastructural contributions to the study of the glassy-winged sharpshooter and Pierce's Disease. Proceedings of CDFA Pierce's Disease Control Program Research Symposium. p. 100-102.

Parasitism of the Glassy-Winged Sharpshooter, Homalodisca Coagulata (Homoptera: Cicadellidae): Functional Reponses and Superparasitism by Gonatocerus Ashmeadi (Hymenoptera: Mymaridae) - (Peer Reviewed Journal)

Chen, W., Leopold, R.A., Harris, M. 2006. Parasitism of the glassy-winged sharpshooter, Homalodisca coagulata (Homoptera: Cicadellidae): Functional reponse and superparasitism by Gonatocerus ashmeadi (Hymenoptera: Mymaridae). Biological Control. 37(1):119-129.

The Influence of Temperature on Development and Reproduction of the Egg Parasitoid, Gonatocerus Ashmeadi Girault (Hymenoptera: Mymaridae) - (Proceedings/Symposium)

Leopold, R.A., Chen, W., Morgan, D.J. 2005. The influence of temperature on development and reproduction of the egg parasitoid, Gonatocerus ashmeadi Girault (Hymenoptera: Mymaridae). Proceedings of 2005 CDFA Pierce's Disease Control Program Research Symposium. Dec. 5-7, 2005. San Diego, CA. pp. 349-353.

Refrigerated Storage of Homalodisca Coagulata Eggs Used for Propagation of the Parasitoid, Gonatocerus Ashmeadi (Hymenoptera: Mymaridae) - (Proceedings/Symposium)

Leopold, R.A., Chen, W. 2005. Refrigerated storage of Homalodisca coagulata eggs used for propagation of the parasitoid, Gonatocerus ashmeadi (Hymenoptera: Mymaridae). Proceedings of the 2005 CDFA Pierce's Disease Control Program Research Symposium. Dec. 5-7, 2005. San Diego, CA. pp. 354-358.

Differential Gene Expression in Mexican Fruit Flies after Cryopreservation. - (Abstract)

Rajamohan, A., Yocum, G.D., Leopold, R.A. 2005. Differential gene expression in Mexican fruit flies after cryopreservation [abstract]. Cryobiology. 51(3):406.

Refrigerated Storage of Glassy-Winged Sharpshooter Eggs for Use in the Propagation of Parasitoids - (Abstract)

Chen, W., Harris, M., Leopold, R.A. 2005. Refrigerated storage of glassy-winged sharpshooter eggs for use in the propagation of parasitoids [abstract]. Cryobiology. 51(3):406-407.

Colony Maintenance and Mass-Rearing: Using Cold Storage Technology for Extending the Shelf-Life of Insects. - (Abstract)

Leopold, R.A. 2005. Colony maintenance and mass-rearing: Using cold storage technology for extending the shelf-life of insects. FAO/IAEA International Conference on Area-wide Control of Insect Pests: Integrating the sterile insect and related nuclear and other techniques. May 9-13, 2005, Vienna, Austria. In: Book of Extended Synopses. pp. 100-103.

Parasitism of the Glassy-Winged Sharpshooter, Homalodisca Coagulata: Functional Responses and Super-Parasitism by the Egg Parasitoid, Gonatocerus Ashmeadi - (Proceedings/Symposium)

Leopold, R.A., Chen, W. 2004. Parasitism of the glassy-winged sharpshooter: Functional responses and super-parasitism by the egg parasitoid, Gonatocerus ashmeadi. In: CDFA Pierce's Disease Control Program Research Symposium, December 7-10, 2004, Coronado, California. p. 128-131.

Effects of Constant and Cyclic Stepwise-Increasing Temperatures on Parasitized and Unparasitzed Eggs of the Glassy-Winged Sharpshooter, Homalodisca Coagulata, During Cold Storage - (Proceedings/Symposium)

Leopold, R.A., Chen, W., Yocum, G.D. 2004. Effects of using constant and cyclical stepwise-increasing temperatures on parasitized and unparasitzed eggs of the glassy-winged sharpshooter during cold storage. In: Proceedings of the CDFA Pierce's Disease Control Program Research Symposium, December 7-10, 2004, Coronado, California. p. 124-127.

Host Selection and Low Temperature Storage of the Glassy-Winged Sharpshooter, Homalodisca Coagulata - (Abstract)

Leopold, R.A., Yocum, G.D., Morgan, D.W., Chen, W., Lauziere, I. 2004. Host selection and low temperature storage of the glassy-winged sharpshooter, homalodisca coagulata [abstract]. CDFA Pierce's Disease Control Program Research Symposium. pp. 90-92.

Cryopreservation of Dipteran Insects: Development and Evaluation - (Abstract)

Leopold, R.A. 2004. Cryopreservation of dipteran insects: development and evaluation. Meeting Abstract. October 18-23, 2004. St. Petersburg, Russia. In: Materials of the International Conference "Preservation of Genetic Resources" 46(9):760-761.

Establishing a National Insect Germplasm Storage Program for the U.S.A. - (Abstract)

Leopold, R.A. Establishing a national insect germplasm storage program for the U.S.A.. Meeting Abstract. Leopold, R.A. 2004. Cryopreservation of dipteran insects: development and evaluation. Meeting Abstract.October 18-23, 2004. St. Petersburg, Russia. In: Materials of the International Conference "Preservation of Genetic Resources" 46(9):760.

Cryopreservation of Non-Mammalian Metazoan Systems - (Book/Chapter)

Leopold, R.A. 2007. Cryopreservation of non-mammalian metazoan systems. 2006 Leopold, R.A. Cryopreservation of non-mammalian metazoan systems. In: Baust, J.G., Baust J.M. editors. Advances in Biopreservation. Boca Raton, FL. CRC Press, Taylor & Francis Group. p. 271-298.

Ultrastructural Contributions to the Study of the Glassy-Winged Sharpshooter and Pierce's Disease - (Proceedings/Symposium)

Freeman, T.P., Leopold, R.A., Nelson, D.R., Buckner, J.S., Henneberry, T.J. 2003. Ultrastructural contributions to the study of the glassy-winged sharpshooter and pierce's disease. CDFA Pierce's Disease Control Program Research Symposium. pp. 215-216.

Development and Results of Quality Assurance Testing for Mass-Reared and Laboratory-Colonized Insects after Embryo Cryopreservation - (Abstract)

Leopold, R.A., Rajamohan, A., Shelly, T.E. 2003. Development and results of quality assurance testing for mass-reared and laboratory-colonized insects after embryo cryopreservation. Abstracts/Cryobiology. 47(3):270.

Development and Results of Quality Assurance Testing for Mass-Reared and Laboratory-Colonized Insects after Storage in Liquid Nitrogen - (Abstract)

Leopold, R.A., Rajamohan, A., Shelly, T.E. 2003. Development and results of quality assurance testing for mass-reared and laboratory-colonized insects after storage in liquid nitrogen. 10th IOBC Arthropod Mass Rearing & Quality Control Workshop, Montpelier, France. In: Global IOBC Bulletin No. 2, pp. 29. 2003.

Composition of the Surface Hydrocarbons from the Vitelline Membrane of Dipteran Embryos. - (Peer Reviewed Journal)

NELSON, D.R., LEOPOLD, R.A. COMPOSITION OF THE SURFACE HYDROCARBONS FROM THE VITELLINE MEMBRANE OF DIPTERAN EMBRYOS. COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY. 2003. v. 136(2)B. p. 295-308.

Mouthpart Morphology and Stylet Penetration of Host Plants by the Glassy-Winged Sharpshooter, Homalodisca Coagulata, (Homoptera: Cicadellidae) - (Peer Reviewed Journal)

LEOPOLD, R.A., FREEMAN, T.P., BUCKNER, J.S., NELSON, D.R. MOUTHPART MORPHOLOGY AND STYLET PENETRATION OF HOST PLANTS BY THE GLASSY-WINGED SHARPSHOOTER, HOMALODISCA COAGULATA, (HOMOPTERA: CICADELLIDAE). Arthropod Structure and Development. 2003. v. 32. p. 189-199.

Cryopreservation of Mediterranean Fruit Fly Embryos - (Peer Reviewed Journal)

RAJAMOHAN,A., LEOPOLD,R.A., WANG,W.B., HARRIS,M., MCCOMBS,S.D., PEABODY,N.C., FISHER,K., CRYOPRESERVATION OF MEDITERRANEAN FRUIT FLY EMBRYOS, CRYOLETTERS 24:125-132, 2003.

Analysis of the Genetic Variation of Mexican Fruit Flies Before and after Embryo Cryopreservation - (Abstract) - (18-Jul-02)

Vitrification of Mexican Fruit Fly Embryos - (Abstract)

Rajamohan, A., Leopold, R.A., Harris, M. 2002. Vitrification of mexican fruit fly embryos [abstract]. Society for Cryobiology Meeting. Abstracts/Cryobiology 45:247.

Long-Term Cold Storage of Medfly Embryos - (Abstract) - (15-May-02)

Cryopreservation of New World Screwworm Embryos - (Other) - (04-May-02)

Ultrastructure of Glassy-Winged Sharpshooter Mouthparts and Salivary Sheaths - (Abstract) - (27-Mar-02)