Projects

DETECTION OF TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHY AGENTS IN LIVESTOCK, WILDLIFE, AGRICULTURAL PRODUCTS, AND THE ENVIRONMENT Appropriated (D)   Accession Number: 413072

Immunodiagnostics to Detect Prions and Other Important Animal Pathogens Appropriated (D)   Accession Number: 424354

Using Mass Spectrometry to Detect Priorns in Cell Lines Nonfunded Cooperative Agreement (N)   Accession Number: 419631

Identification of Protein Interactions via Mass Spectroscopy Trust (T)   Accession Number: 416753

Publications (Clicking on the reprint icon will take you to the publication reprint.)

Oxidation of methionine 216 in sheep and elk prion protein is highly dependent upon the amino acid at position 218 but is not important for prion propagation - (Peer Reviewed Journal)

Silva, C.J., Dynin, I.A., Erickson-Beltran, M.L., Requena, J.R., Balachandran, A., Onisko, B.C., Hui, C., Carter, J.M. 2013. Using mass spectrometry to detect prions and oxidized prions in scrapie-infected sheep and CWD-infected elk. Biochemistry. 52:2139-2147. doi:10.1021/bi3016795.

Structural organization of mammalian prions as probed by limited proteolysis - (Peer Reviewed Journal)

Vasquez-Fernandez, E., Alonso, J., Pastrana, M.A., Ramos, A., Stitz, L., Vidal, E., Dynin, I.A., Petsch, B., Silva, C.J., Requena, J.R. 2012. Structural organization of mammalian prions as probed by limited proteolysis. PLoS One. 7(11):e50111. doi:10.1371/journal.pone.0050111.

Detecting and discriminating among pathogenic protein conformers(prions), using mass spectrometry-based and antibody-based approaches(Abstract) - (Abstract Only)

Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson-Beltran, M.L., Hui, C., Carter, J.M. 2012. Detecting and discriminating among pathogenic protein conformers(prions), using mass spectrometry-based and antibody-based approaches(Abstract). Meeting Abstract. Poster 216.

Using synthetic small molecule reagents and antibodies to distinguish among PrP conformers: new uses for old antibodies - (Abstract Only)

Silva, C.J., Erickson-Beltran, M.L., Dynin, I.A., Carter, J.M. 2012. Using synthetic small molecule reagents and antibodies to distinguish among PrP conformers: new uses for old antibodies. Meeting Abstract. Prion 6(supplement),90.

Probing the structure of GPI-less PrPSc by limited proteolysis(Abstract) - (Abstract Only)

Vazquez-Fernandez, E., Pastrana, M., Ramos, A., Alonso, J., Stitz, L., Vindal, E., Dynin, I.A., Silva, C.J. 2012. Probing the structure of GPI-less PrPSc by limited proteolysis(Abstract). Meeting Abstract. Prion 6(supplement),26-27.

Molecular approaches to detecting and discriminating among prions, a class of pathogenic molecules(Abstract) - (Abstract Only)

Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson-Beltran, M.L., Hui, C., Carter, J.M. 2012. Molecular approaches to detecting and discriminating among prions, a class of pathogenic molecules(Abstract). Meeting Abstract. Poster, 615.

Human and rat brain lipofuscin proteome - (Peer Reviewed Journal)

Otts, P., Koppe, K., Onisko, B.C., Dynin, I.A., Arzberger, T., Kretzschmar, H., Requena, J.R., Silva, C.J., Huston, J.P., Korth, C. 2012. Human and rat brain lipofuscin proteome. Proteomics. 12(15-16):2445-2454. doi:10.1002/pmic.201100668.

PK-sensitive PrPSc is infectious and shares basic structural features with PK-resistant PrPSc - (Peer Reviewed Journal)

Sajnani, G., Silva, C.J., Ramos, A., Pastrana, M.A., Onisko, B.C., Erickson-Beltran, M.L., Antaki, E.M., Sigurdson, C.J., Carter, J.M., Requena, J.R. 2012. PK-sensitive PrPSc is infectious and shares basic structural features with PK-resistant PrPSc. PLoS Pathogens. 8(3):e1002547. doi:10.1371/journal.ppat.1002547.

Using small molecule reagents to selectively modify epitopes based on their conformation - (Peer Reviewed Journal)

Silva, C.J. 2012. Using small molecule reagents to selectively modify epitopes based on their conformation. Prion. 6:(2)165-175.

Using Mass Spectrometry to Diagnose Prion diseases: Can we do that? - (Abstract Only) - (16-May-11)

Diagnosing Prion Diseases: Mass Spectrometry-Based Approaches - (Abstract Only)

Silva, C.J., Erickson, M.L., Dynin, I.A., Onisko, B.C., Carter, J.M. 2011. Diagnosing Prion Diseases: Mass Spectrometry-Based Approaches. Meeting Abstract. Prion, Volume 5 (Supplement), April/May/June 2011. page 85.

Probing structural differences between PrPC and PrPSc by surface nitration and acetylation: evidence of conformational change in the C-terminus - (Peer Reviewed Journal)

Gong, B., Ramos, A., Vazquez-Fernandez, E., Silva, C.J., Alonso, J., Requena, J.R. 2011. Probing structural differences between PrPC and PrPSc by surface nitration and acetylation: evidence of conformational change in the C-terminus. Biochemistry. 50:4963-4972.

Utility of mass spectrometry in the diagnosis of prion diseases - (Peer Reviewed Journal)

Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson-Beltran, M.L., Requena, J.J., Carter, J.M. 2011. Utility of mass spectrometry in the diagnosis of prion diseases. Analytical Chemistry. 83(5):1609-1615. doi:10.1021/ac102527w.

Mass spectrometry and prions: The need to simplify and remove oil from the system - (Review Article)

Silva, C.J. 2010. Mass spectrometry and prions: The need to simplify and remove oil from the system. International News on Fats, Oils and Related Materials.21(8):517-520.

Detecting and quantifying prions: Mass spectrometry-based approaches - (Abstract Only) - (20-Apr-10)

Assessing the Role of Oxidized Methionine at Position 213 in the Formation of Prions in Hamsters - (Peer Reviewed Journal)

Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson, M.L., Vensel, W.H., Requena, J., Antaki, E.M., Carter, J.M. 2010. Assessing the Role of Oxidized Methionine at Position 213 in the Formation of Prions in Hamsters. Journal of Biochemistry. (49):1854-1861.

Mass Spectrometry of Prions: Approaches to Conformational Distinction - (Abstract Only)

Silva, C.J., Onisko, B., Dynin, I.A., Erickson, M.L., Carter, J.M. 2009. Mass Spectrometry of Prions: Approaches to Conformational Distinction. [Abstract]. AOAC 123rd Annual Meeting & Exposition. S-902. p68-69

Disinfectants and Prions - (Abstract Only)

Silva, C.J. 2009. Disinfectants and Prions. [Abstract]. AOAC Annual Meeting & Exposition S-401. p63

Induction of Purple Sulfur Bacterial Growth in Dairy Wastewater Lagoons by Circulation - (Peer Reviewed Journal)

Mcgarvey, J.A., Miller, W.G., Lathrop, J.R., Silva, C.J., Bullard, G. 2009. Induction of Purple Sulfur Bacterial Growth in Dairy Wastewater Lagoons by Circulation. Letters in Applied Microbiology. 49:427-433.

Prion Infectivity Assays. - (Government Publication) - (30-Mar-09)

Mass Spectrometric Approaches to Detecting and Quantifying Prions - (Abstract Only)

Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson, M.L. 2009. Mass Spectrometric Approaches to Detecting and Quantifying Prions. [Abstract]. PrP Canada p28.

Prion diseases: A little prevention can prevent a catastrophe - (Abstract Only)

Silva, C.J. 2009. Prion diseases: A little prevention can prevent a catastrophe. [Abstract]. AOAC AM-1 p1.

Prions: The Chemistry of Infectious Proteins - (Abstract Only)

Silva, C.J. 2008. Prions: The Chemistry of Infectious Proteins [Abstract]. American Oil Chemists' Society. S-401. p.63.

Mass spectrometric approaches to detecting prions and protein conformers - (Abstract Only)

Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson, M.L. 2009. Mass spectrometric approaches to detecting prions and protein conformers. [Abstract] AOAC S-401. p63

Prion Diseases - (Book / Chapter)

Silva, C.J. 2009.Prion Diseases:Sequelae and Long Term Consequences of Infectious Diseases. District of Columbia:ASM Press. 442p.

Mass Spectrometric Approaches to Detecting and Quantifying Prions - (Abstract Only)

Silva, C.J., Onisko, B.C., Dynin, I.A., Erickson, M.L., Carter, J.M. 2008. Mass Spectrometric Approaches to Detecting and Quantifying Prions. [Abstract]. Third International CWD Symposium. Advancing the Science and Developing the Tools. Vol 3. p23.

Bovine Spongiform Encephalopathy - (Review Article)

Harman, J.L., Silva, C.J. 2009. Bovine Spongiform Encephalopathy. Journal of the American Veterinary Medical Association. (2009)234(1):59-72

Sensitive, Preclinical Detection of Prions in Brain by nanospray liquid chromatography/tandem mass spectrometry - (Peer Reviewed Journal)

Onisko, B.C., Silva, C.J., Dynin, I.A., Erickson, M.L., Vensel, W.H., Hnasko, R.M., Requena, J.R., Carter, J.M. 2007. Sensitive, Preclinical Detection of Prions in Brain by nanospray liquid chromatography/tandem mass spectrometry. Rapid Communications in Mass Spectrometry. 21(24):4023-4026.

Pathobiology and diagnosis of animal transmissible spongiform encephalopathies: current knowledge, research gaps, and opportunities - (Government Publication)

Kehrli, Jr., M.E., O'Rourke, K.I., Hamir, A.N., Richt, J.A., Nicholson, E.M., Silva, C.J., Edelman, D., Gay, C.G. 2007. Pathobiology and diagnosis of animal transmissible spongiform encephalopathies: current knowledge, research gaps, and opportunities [government white paper]. Beltsville, MD: Interagency Working Group on Prion Science, Subcommittee on Pathobiology and Diagnostics. USDA, Agriculture Research Service. 33 p.

Mass Spectrometric Detection of Attomole Amounts of the Prion Protein by nanoLC-MS-MS - (Peer Reviewed Journal)

Onisko, B.C., Dynin, I.A., Requena, J.R., Silva, C.J., Erickson, M.L., Carter, J.M. 2007. Mass Spectrometric Detection of Attomole Amounts of the Prion Protein by nanoLC-MS-MS. Journal of American Society for Mass Spectrometry. Volume(18); 1070-1079

State of BSE in the United States - (Trade Journal)

Silva, C.J. 2007. State of BSE in the United States. The National Provisioner. April 2007: Page 15. http://www.provisioneronline.com/content.php?s=NP/2007/04&p=15

MASS SPECTROMETRIC DETECTION OF ATTOMOLE AMOUNTS OF THE PRION PROTEIN, PRP 27-30, BY NANOLC-MS-MS - (Abstract Only)

Onisko, B.C., Requena, J., Silva, C.J., Dynin, I.A., Carter, J.M. 2006. Mass spectrometric detection of attomole amounts of the prion protein, prp 27-30, by nanolc-ms-ms. [Abstract]. 232nd ACS National Meeting and Exposition. Poster AGFD 200

PRIONS: PATHOLOGICAL PROTEINS AT THE INTERFACE OF OIL AND WATER - (Abstract Only)

Silva, C.J. 2006. Prions: pathological proteins at the interface of oil and water [Abstract]. 97th AOCS Annual Meeting & Expo, April 30-May3, 2006, St. Louis, MO. Abstract # FM3-1; page 43. Available: http://www.aocs.org/archives/am2006/session.asp?session=FM+3%3A+Animal%2DHuman+Linked+Health+and+Nutrition+Issues

PRIONS: PATHOLOGICAL PROTEINS AT THE INTERFACE OF OIL AND WATER. - (Review Article)

Silva, C.J. 2006. Prions: pathological proteins at the interface of oil and water. Inform. 17(2), pp. 76-77+79.

HETEROLOGOUS PRODUCTION OF DAPTOMYCIN IN STREPTOMYCES LIVIDANS - (Peer Reviewed Journal)

Penn, J., Li, X., Whiting, A., Latif, M., Silva, C.J., Brian, P., Davies, J., Miao, V., Wrigley, S.K., Baltz, R.H. 2005. Heterologous production of daptomycin in streptomyces lividans [Epub ahead of print]. Journal of Industrial Microbiology and Biotechnology. Nov 1:1-8.

BACTERIAL POPULATION STRUCTURE OF DAIRY WASTEWATERS - (Abstract Only)

Mcgarvey, J.A., Miller, W.G., Sanchez, S., Silva, C.J. 2005. Bacterial population structure of dairy wastewaters [abstract]. International Union of Microbiological Societies, July 23-28, 2005, San Francisco, CA. Poster No. B1102.

BACTERIAL AND CHEMICAL COMPOSITION OF DAIRY WASTEWATER - (Abstract Only)

Mcgarvey, J.A., Miller, W.G., Sanchez, S., Silva, C.J. 2005. Bacterial and chemical composition of dairy wastewater [abstract]. American Society for Microbiology General Meeting, 6/5-6/9/05, Atlanta, GA. P. 008.

COMPARISON OF BACTERIAL POPULATIONS AND CHEMICAL COMPOSITION OF DAIRY WASTEWATER HELD IN CIRCULATED AND STAGNANT LAGOONS - (Peer Reviewed Journal)

Mcgarvey, J.A., Miller, W.G., Sanchez, S., Silva, C.J., Whitehand, L.C. 2005 Comparison of bacterial populations and chemical composition of dairy wastewater held in circulated and stagnant lagoons [abstract]. Journal of Applied Microbiology. 99(10):867-877.

DAPTOMYCIN BIOSYNTHESIS IN STREPTOMYCES ROSEOSPORUS: CLONING AND ANALYSIS OF THE GENE CLUSTER AND REVISION OF PEPTIDE STEREOCHEMISTRY - (Peer Reviewed Journal)

Miao, V., Coeffet-LeGal, M-F, Brian, P., Brost, R., Penn, J., Whiting, A., Martin, S. Ford, R., Parr, I., Bouchard, M., Silva, C.J., Wrigley, S.K., Baltz, R.H. 2005. Daptomycin biosynthesis in Streptomyces roseosporus: cloning and analysis of the gene cluster and revision of peptide stereochemistry. Microbiology. 151(5):1507-1523.

SCRAPIE: THE VERY MODEL OF AN INFECTIOUS(PROTEIN)ISOFORM - (Abstract Only)

Silva, C.J. 2005. Scrapie: The Very Model of an Infectious (Protein) Isoform [abstract]. 96th AOCS Annual Meeting & Expo. Salt Lake City, UT, May 2, 2005.

PRIONS: THE TWISTED TALE OF INFECTIOUS PROTEIN ISOFORMS - (Other)

Silva, C.J. 2004. Prions: The Twisted Tale of Infectious Protein Isoforms. The Vortex. American Chemical Society, California Section. 65(6):4.

CHOLESTEROL: A MARKER FOR THE PRESENCE OF ANIMAL MATERIAL IN FEED - (Abstract Only) - (17-Jan-03)