Projects

Prevention and Control of Shiga-Toxigenic Escherichia Coli in Livestock Appropriated (D)   Accession Number: 410408

Control of Pathogenic and Spoilage Bacteria on Red Meat Appropriated (D)   Accession Number: 410528

Prevention of Zoonotic Pathogen Transmission from Animal Manure to Human Food Appropriated (D)   Accession Number: 409875

Publications

Evaluation of a Direct Feed Microbial Product Effect on the Prevalence and Load of Escherichia coli O157:H7 in Feedlot Cattle - (Peer Reviewed Journal) - (14-Oct-09)

Evaluation of the Fate of Naturally Occurring Escherichia coli O157:H7 During Bovine Manure Composting Processes - (Abstract) - (03-Aug-09)

The Effects of Dietary Additives on Faecal Levels of Lactobacillus spp., Coliforms, and Escherichia coli, and Faecal Prevalence of Salmonella spp. and Campylobacter spp. in U.S. Production Nursery Swine - (Peer Reviewed Journal) - (24-May-09)

Methane Emission by Goats Consuming Condensed Tannin-containing Forage at Different Frequencies - (Abstract)

Puchala, R., Animut, G., Goetsch, A.L., Sahlu, T., Varel, V.H., Wells, J. 2009. Methane Emission by Goats Consuming Condensed Tannin-containing Forage at Different Frequencies [abstract]. Journal of Animal Science 87 (E-Supplement 2):483.

Prevalence and Level of Escherichia coli O157:H7 in Feces and on Hides of Feedlot Steers Fed Diets With or Without Wet Distillers Grains with Solubles - (Peer Reviewed Journal)

Wells, J., Shackelford, S.D., Berry, E.D., Kalchayanand, N., Guerini, M.N., Varel, V.H., Arthur, T.M., Bosilevac, J.M., Freetly, H.C., Wheeler, T.L., Ferrell, C.L., Koohmaraie, M. 2009. Prevalence and Level of Escherichia coli O157:H7 in Feces and on Hides of Feedlot Steers Fed Diets With or Without Wet Distillers Grains with Solubles. Journal of Food Protection 72(8):1624-1633.

Prevalence of Mycobacterium avium subsp. paratuberculosis in Ileocecal Lymph Nodes and on Hides and Carcasses from Cull Cows and Fed Cattle at Commercial Beef Processing Plants in the United States - (Peer Reviewed Journal)

Wells, J., Bosilevac, J.M., Kalchayanand, N., Arthur, T.M., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2009. Prevalence of Mycobacterium avium subsp. paratuberculosis in Ileocecal Lymph Nodes and on Hides and Carcasses from Cull Cows and Fed Cattle at Commercial Beef Processing Plants in the United States. Journal of Food Protection 72(7):1457-1462.

Prevalence and Level of Escherichia coli O157:H7 in Feces and On Hides of Feedlot Steers Fed Diets With or Without Wet Distillers Grains With Solubles - (Abstract)

Wells, J., Shackelford, S.D., Berry, E.D., Kalchayanand, N., Varel, V.H., Arthur, T.M., Bosilevac, J.M., Freetly, H.C., Wheeler, T.L., Ferrell, C.L., Koohmaraie, M. 2009. Prevalence and Level of Escherichia coli O157:H7 in Feces and On Hides of Feedlot Steers Fed Diets With or Without Wet Distillers Grains With Solubles [abstract]. Microbial Ecology 57(3):583-584.

Prevalence of Clostridium difficile from Commercial Beef Processing Plants in the United States - (Abstract)

Kalchayanand, N., Arthur, T.M., Bosilevac, J.M., Harhay, D.M., Shackelford, S.D., Wells, J., Wheeler, T.L., Koohmaraie, M. 2009. Prevalence of Clostridium difficile from Commercial Beef Processing Plants in the United States [abstract]. Institute of Food Technologists (IFT), Division Food Microbiology, Session 026-15, p. 82.

Escherichia coli O157:H7 and Salmonella in Small Processing Plants and the Effect of Feeding Wet Distillers Grains with Solubles on E. coli O157:H7 - (Abstract)

Bosilevac, J.M., Wells, J., Arthur, T.M., Kalchayanand, N., Shackelford, S.D., Berry, E.D., Bono, J.L., Harhay, D.M., Wheeler, T.L., Koohmaraie, M. 2009. Escherichia coli O157:H7 and Salmonella in Small Processing Plants and the Effect of Feeding Wet Distillers Grains with Solubles on E. coli O157:H7 [abstract]. NCBA Beef Industry Safety Summit, San Diego, CA, March 4-6, 2009.

Impacts of Individual Animal Response to Heat and Handling Stresses on Escherichia coli and E. coli O157:H7 Fecal Shedding by Feedlot Cattle - (Peer Reviewed Journal)

Brown Brandl, T.M., Berry, E.D., Wells, J., Arthur, T.M., Nienaber, J.A. 2009. Impacts of Individual Animal Response to Heat and Handling Stresses on Escherichia coli and E. coli O157:H7 Fecal Shedding by Feedlot Cattle. Foodborne Pathogens and Disease 6(7):855-864.

Odorant production and persistence of Escherichia coli in manure slurries from cattle fed zero, twenty, forty, or sixty percent wet distillers grains with solubles - (Peer Reviewed Journal)

Varel, V.H., Wells, J., Berry, E.D., Spiehs, M.J., Miller, D.N., Ferrell, C.L., Shackelford, S.D., Koohmaraie, M. 2008. Odorant production and persistence of Escherichia coli in manure slurries from cattle fed zero, twenty, forty, or sixty percent wet distillers grains with solubles. Journal of Animal Science 86:3617-3627.

A direct plating method for estimating populations of Escherichia coli 0157 in bovine manure and manure-based materials - (Peer Reviewed Journal)

Berry, E.D., Wells, J. 2008. A direct plating method for estimating populations of Escherichia coli 0157 in bovine manure and manure-based materials. Journal of Food Protection 71(11):2233-2238.

The impact of stress level on fecal bacteria and pathogen shedding in feedlot cattle. - (Proceedings/Symposium)

Brown Brandl, T.M., Berry, E.D., Wells, J., Arthur, T.M., Nienaber, J.A. 2008. The impact of stress level on fecal bacteria and pathogen shedding in feedlot cattle. American Society of Agricultural and Biological Engineers. Paper No. 084178. St. Joseph, MI:ASABE.

Ruminal Methane Emissions by Goats Consuming Dry Hay of Condensed Tannin-Containing Lespedeza With or Without Polyethylene Glycol, Alfalfa, or Sorghum-Sudangrass - (Abstract)

Puchala, R., Animut, G., Goetsch, A.L., Patra, A.K., Sahlu, T., Varel, V.H., Wells, J. 2008. Ruminal Methane Emissions by Goats Consuming Dry Hay of Condensed Tannin-Containing Lespedeza With or Without Polyethylene Glycol, Alfalfa, or Sorghum-Sudangrass [abstract]. In: Proceedings of 9th International Conference on Goats, Aug. 31 - Sept. 5, 2008, Queretaro, Mexico. Abstract 73, pp. 98-99.

Prevalence and Characterization of Salmonella in Bovine Lymph Nodes Potentially Destined for Use in Ground Beef - (Peer Reviewed Journal)

Arthur, T.M., Harhay, D.M., Bosilevac, J.M., Guerini, M.N., Kalchayanand, N., Wells, J., Shackelford, S.D., Wheeler, T.L., Koohmaraie, M. 2008. Prevalence and characterization of Salmonella in bovine lymph nodes potentially destined for use in ground beef. Journal of Food Protection 71(8):1685-1688.

Odorant production and persistence of generic E. coli in manure slurries from cattle fed 0, 20, 40, and 60% wet distillers grains with solubles (WDGS). - (Abstract)

Varel, V.H., Wells, J., Berry, E.D., Spiehs, M.J., Miller, D.N., Ferrell, C.L., Shackelford, S.D., Koohmaraie, M. 2008. Odorant production and persistence of generic E. coli in manure slurries from cattle fed 0, 20, 40, and 60% wet distillers grains with solubles (WDGS)[abstract]. Journal of Animal Science 86(E-Suppl. 2)/Journal of Dairy Science 91(E-Suppl. 1):325.

Viability of zoonotic pathogens Escherichia coli and Salmonella in swine manure slurries with and without a urease inhibitor and thymol - (Peer Reviewed Journal)

Wells, J., Varel, V.H. 2008. Viability of zoonotic pathogens Escherichia coli and Salmonella in swine manure slurries with and without a urease inhibitor and thymol. Letters in Applied Microbiology 46:477-482.

Methane emission by goats consuming diets with different levels of condensed tannins from lespedeza - (Peer Reviewed Journal)

Animut, G., Puchala, R., Goetsch, A.L., Patra, A.K., Sahlu, T., Varel, V.H., Wells, J. 2008. Methane emission by goats consuming diets with different levels of condensed tannins from lespedeza. Animal Feed Science and Technology 144:212-227.

Methane emission by goats consuming different sources of condensed tannins - (Peer Reviewed Journal)

Animut, G., Puchala, R., Goetsch, A.L., Patra, A.K., Sahlu, T., Varel, V.H., Wells, J. 2008. Methane emission by goats consuming different sources of condensed tannins. Animal Feed Science and Technology 144:228-241.

Incidence and persistence of zoonotic bacterial and protozoan pathogens in a beef cattle feedlot runoff control - vegetative treatment system - (Peer Reviewed Journal)

Berry, E.D., Woodbury, B.L., Nienaber, J.A., Eigenberg, R.A., Thurston Enriquez, J.A., Wells, J. 2007. Incidence and persistence of zoonotic bacterial and protozoan pathogens in a beef cattle feedlot runoff control - vegetative treatment system. Journal of Environmental Quality 36:1873-1882.

Effect of N-(n-butyl) Phosphoric Triamide (NPBT) and a Linalool or Pine Oil Extract on Urea Concentration, Odorants, and Coliform Bacteria in Cattle Feedlot Manure - (Proceedings/Symposium)

Varel, V.H., Wells, J., Berry, E.D. 2007. Effect of N-(n-butyl) Phosphoric Triamide (NPBT) and a Linalool or Pine Oil Extract on Urea Concentration, Odorants, and Coliform Bacteria in Cattle Feedlot Manure. In CDRom Proceedings: American Society of Agri Engineers Special Meetings, Sept. 15-19, 2007, Broomfield, CO. CDRom 701P0907cd.

Combination of a Urease Inhibitor and a Plant Essential Oil to Control Coliform Bacteria, Odour Production, and Ammonia Loss from Cattle Waste - (Peer Reviewed Journal)

Varel, V.H., Wells, J., Miller, D.N. 2007. Combination of a urease inhibitor and a plant essential oil to control coliform bacteria, odour production, and ammonia loss from cattle waste. Journal of Applied Microbiology 102:472-477. doi:10.1111/j.1365-2672.2006.03120.x

Influence of Thymol and a Urease Inhibitor on Coliform Bacteria, Odor, Urea, and Methane from a Swine Production Manure Pit - (Peer Reviewed Journal)

Varel, V.H., Wells, J. 2007. Influence of thymol and a urease inhibitor on coliform bacteria, odor, urea, and methane from a swine production manure pit. Journal of Environmental Quality 36(3):773-779.

Influence of Genotype and Diet on Steer Performance, Manure Odor, and Carriage of Pathogenic and Other Fecal Bacteria. Ii. Pathogenic and Other Fecal Bacteria - (Peer Reviewed Journal)

Berry, E.D., Wells, J.E., Archibeque, S.L., Ferrell, C.L., Freetly, H.C., Miller, D.N. 2006. Influence of genotype and diet on steer performance, manure odor, and carriage of pathogenic and other fecal bacteria. II. Pathogenic and other fecal bacteria. Journal of Animal Science. 84:2523-2532.

Influence of Genotype and Diet on Steer Performance, Manure Odor, and Carriage of Pathogenic and Other Fecal Bacteria. Iii. Odorous Compound Production - (Peer Reviewed Journal)

Miller, D.N., Berry, E.D., Wells, J.E., Ferrell, C.L., Archibeque, S.L., Freetly, H.C. 2006. Influence of genotype and diet on steer performance, manure odor, and carriage of pathogenic and other fecal bacteria. III. Odorous compound production. Journal of Animal Science. 84:2533-2545.

Methane Emission by Goats Consuming Diets with Different Levels of Condensed Tannin-Containing Lespedeza and Sorghum-Sudangrass - (Abstract)

Animut, G., Puchala, R., Goetsch, A., Sahlu, T., Detweiler, G., Patra, A., Varel, V., Wells, J. 2006. Methane emission by goats consuming diets with different levels of condensed tannin-containing lespedeza and sorghum-sudangrass [abstract]. Journal of Animal Science 84(Suppl. 1):26.

Influence of Thymol on Coliform Bacteria, Vfa, and Methane Production from Pull-Plug Swine Manure Pits - (Abstract)

Varel, V.H., Wells, J. 2006. Influence of thymol on coliform bacteria, VFA, and methane production from pull-plug swine manure pits [abstract]. Journal of Animal Science 84(Suppl. 1):292-293.

Effects of Essential Oils on Viability of Escherichia Coli O157:h7 in Treated Beef Cattle Manure Slurries and on Prevalence from Treated Feedlot Surfaces - (Abstract)

Wells, J., Berry, E.D., Varel, V.H. 2006. Effects of essential oils on viability of Escherichia coli O157:H7 in treated beef cattle manure slurries and on prevalence from treated feedlot surfaces [abstract]. Journal of Animal Science 84(Suppl 1):356-357.

Effects of Common Forage Phenolic Acids on Escherichia Coli O157:h7 Viability in Bovine Feces - (Peer Reviewed Journal)

Wells, J.E., Berry, E.D., Varel, V.H. 2005. Effects of common forage phenolic acids on Escherichia coli O157:H7 viability in bovine feces. Applied and Environmental Microbiology. 71(12)7974-7979.

Viability of Salmonella Enterica Typhimurium and Escherichia Coli O157:h7 in Finishing Swine Manure Slurries with and Without a Urease Inhibitor and a Plant Essential Oil - (Abstract)

Wells, J., Varel, V.H. 2005. Viability of Salmonella enterica Typhimurium and Escherichia coli O157:H7 in finishing swine manure slurries with and without a urease inhibitor and a plant essential oil [abstract]. Journal of Animal Science. 83(Supplement 1):301-302.

The Effects of Larch Extract on Performance and Fecal Flora in Nursery Pigs - (Abstract)

Wells, J., Yen, J. 2005. The effects of larch extract on performance and fecal flora in nursery pigs. Proceedings of the 2005 Conference on Gastrointestinal Function. p. 18.

Plant Oils and Urease Inhibitors As Solutions for Environmental Issues Associated with Confined Animal Feeding Operations - (Abstract)

Varel, V.H., Wells, J. 2005. Plant oils and urease inhibitors as solutions for environmental issues associated with confined animal feeding operations. Proceedings of the 2005 Conference on Gastrointestinal Function. p. 28.

Impact of Dried Skim Milk in Production Diets on Lactobacillus and Pathogenic Bacterial Shedding in Growing-Finishing Swine - (Peer Reviewed Journal)

Wells, J.E., Yen, J.T., Miller, D.N. 2005. Impact of dried skim milk in production diets on lactobacillus and pathogenic bacterial shedding in growing-finishing swine. Journal of Applied Microbiology 99:400-407.

Dried Skim Milk As a Replacement for Soybean Meal in Growing-Finishing Diets: Effects on Growth Performance, Apparent Total-Tract Nitrogen Digestibility, Urinary and Fecal Nitrogen Excretion, and Carcass Traits in Pigs - (Peer Reviewed Journal)

Yen, J., Wells, J., Miller, D.N. 2004. Dried skim milk as a replacement for soybean meal in growing-finishing diets: Effects on growth performance, apparent total-tract nitrogen digestibility, urinary and fecal nitrogen excretion, and carcass traits in pigs. Journal of Animal Science. 82:3338-3345.

Combination of a Urease Inhibitor and a Plant Essential Oil for Control of Fecal Coliforms and Emissions of Odor and Ammonia from Cattle Waste - (Abstract)

Varel, V.H., Wells, J., Miller, D.N. 2004. Combination of a urease inhibitor and a plant essential oil for control of fecal coliforms and emissions of odor and ammonia from cattle waste [abstract]. Journal of Animal Science 82(Suppl. 1):302.

Effect of Dry Skim Milk on Fecal Bacterial Populations and Salmonella Shedding in Growing-Finishing Swine - (Abstract)

Wells, J., Yen, J., Miller, D.N. 2004. Effect of dry skim milk on fecal bacterial populations and salmonella shedding in growing-finishing swine[abstract]. Journal of Animal Science 82(Suppl. 1):174-175.

Effect of Plant Phenolics on Escherichia Coli O157:h7 Survival in Bovine Feces - (Abstract)

Wells, J.E., Berry, E.D., Miller, D.N., Varel, V.H. 2004. Effect of plant phenolics on Escherichia coli O157:H7 survival in bovine feces. In: Proceedings of the 104th General Meeting of the American Society for Microbiology, May 23-27, 2004, New Orleans, Louisiana. 2004 CDROM.

Lower Digestive Tract Microbiology - (Book/Chapter)

Varel, V.H., Wells, J. 2005. Lower digestive tract microbiology. In: Pond, W.G., Bell, A.W., editors. Encyclopedia of Animal Science. Marcel Dekker,Inc., New York, NY. p. 585-587.

Gi Tract: Animal/microbial Symbiosis - (Book/Chapter)

Wells, J., Varel, V.H. 2005. Gi tract: Animal/microbial symbiosis. In: Pond, W.G., Bell, A.W., editors. Encyclopedia of Animal Science. Marcel Dekker,Inc., New York, NY. p. 449-452.

Development and Application of a Polymerase Chain Reaction Assay for the Detection and Enumeration of Bile Acid 7 Alpha-Dehydroxylating Bacteria in Human Feces - (Peer Reviewed Journal)

WELLS, J., WILLIAMS, K.B., WHITEHEAD, T.R., HEUMAN, D.M., HYLEMON, P.B. 2003. DEVELOPMENT AND APPLICATION OF A POLYMERASE CHAIN REACTION ASSAY FOR THE DETECTION AND ENUMERATION OF BILE ACID 7 ALPHA-DEHYDROXYLATING BACTERIA IN HUMAN FECES. CLINICA CHIMICA ACTA. 331:127-134.