Projects

INNOVATIVE PATHOGEN DETECTION AND CHARACTERIZATION TECHNOLOGIES FOR USE IN FOOD SAFETY General Cooperative Agreement (A)   Accession Number: 423341

INNOVATIVE PATHOGEN DETECTION AND CHARACTERIZATION TECHNOLOGIES FOR USE IN FOOD SAFETY Appropriated (D)   Accession Number: 420517

MICROBIAL COMMUNITIES AND INTERACTIONS AND THEIR IMPACT ON FOOD SAFETY Appropriated (D)   Accession Number: 420978

DETECTION AND TYPING OF FOOD-BORNE PATHOGENS Appropriated (D)   Accession Number: 421013

Publications (Clicking on the reprint icon will take you to the publication reprint.)

A high-throughput antibody-based microarray typing platform - (Peer Reviewed Journal)

Gehring, A.G., Barnett, C., Chu, T., Debroy, C., D'Souza, D., Eaker, S., Fratamico, P.M., Gillespie, B., Hedge, N., Jones, K., Lin, J., Oliver, S., Paoli, G., Perera, A., Uknalis, J. 2013. A high-throughput antibody-based microarray typing platform. Sensors. DOI: 10.3390/s130505737.

How does Listeria monocytogenes combat acid conditions? - (Review Article)

Smith, J.L., Liu, Y., Paoli, G. 2012. How does Listeria monocytogenes combat acid conditions?. Canadian Journal of Microbiology. 59:141-152.

Inactivation of F.tularensis Utah-112 on food and food contact surfaces by ultraviolet light - (Peer Reviewed Journal)

Sommers, C.H., Scullen, B.J., Paoli, G., Bhaduri, S. 2012. Inactivation of F.tularensis Utah-112 on food and food contact surfaces by ultraviolet light. Journal of Food Processing and Technology. doi:10.4172/2157-7110.S11-002.

Evaluation of fourier transform infrared (FT-IR) spectroscopy and chemometrics as a rapid approach for subtyping E. coli O157:H7 isolates - (Peer Reviewed Journal)

Davis, R., Paoli, G., Mauer, L.J. 2012. Evaluation of fourier transform infrared (FT-IR) spectroscopy and chemometrics as a rapid approach for subtyping E. coli O157:H7 isolates. Food Microbiology. 31:181-190.

A method for correcting standard-based real-time PCR DNA quantitation when the standard's polymerase reaction efficiency is significantly different from that of the unknown's - (Peer Reviewed Journal)

Irwin, P.L., Nguyen, L.T., Chen, C., Uhlich, G.A., Paoli, G. 2012. A method for correcting standard-based real-time PCR DNA quantitation when the standard's polymerase reaction efficiency is significantly different from that of the unknown's. Analytical and Bioanalytical Chemistry. 402:2713-2725.

Development and validation of a rapid method for microcystins in fish and comparing LC-MS/MS results with ELISA - (Peer Reviewed Journal)

Geis-Asteggiante, L., Lehotay, S.J., Fortis, L.L., Paoli, G., Wijey, C., Heinzen, H. 2011. Development and validation of a rapid method for microcystins in fish and comparing LC-MS/MS results with ELISA. Analytical and Bioanalytical Chemistry. 401:2617-2630.

Emerging foodborne pathogens - (Book / Chapter) - (05-Oct-10)

A POPULATION STUDY OF E. COLI DOUBLING TIMES: EVIDENCE OF AN ANOMALOUS BIMODAL DISTRIBUTION BELOW A THRESHOLD CELL CONCENTRATION - (Peer Reviewed Journal)

Irwin, P.L., Paoli, G., Chen, C., Nguyen, L.T. 2010. A POPULATION STUDY OF E. COLI DOUBLING TIMES: EVIDENCE OF AN ANOMALOUS BIMODAL DISTRIBUTION BELOW A THRESHOLD CELL CONCENTRATION. BMC Microbiology. 10:207.

UV-C Inactivation of Francisella tularensis Utah-112 on agar surfaces, stainless steel, and foods - (Abstract Only)

Sommers, C., Paoli, G., 2010. UV-C Inactivation of Francisella tularensis Utah-112 on agar surfaces, stainless steel, and foods [abstract]. Institute of Food Technologists Annual Meeting. Chicago, IL. p.1.

A real-time PCR method for the detection of Salmonella enterica from food using a target sequence identified by comparative genomic analysis - (Peer Reviewed Journal)

Chen, J., Zhang, L., Paoli, G., Tu, S., Shi, X. 2010. A real-time PCR method for the detection of Salmonella enterica from food using a target sequence identified by comparative genomic analysis. International Journal of Food Microbiology. 137:168-174.

Evaluation of the MIT RMID 1000 system for the identification of Listeria species:AOAC performance tested method 090325 - (Peer Reviewed Journal)

Ricardi, J., Haavig, D., Cruz, L., Paoli, G., Gehring, A.G. 2010. Evaluation of the MIT RMID 1000 system for the identification of Listeria species:AOAC performance tested method 090325. Journal of Association of Official Analytical Chemists International. 93(1):249-258.

Development of an oligonucleotide-based microarray to detect multiple foodborne pathogens - (Peer Reviewed Journal)

Suo, B., He, Y., Paoli, G., Gehring, A.G., Tu, S., Shi, X. 2009. Development of an oligonucleotide-based microarray to detect multiple foodborne pathogens. Molecular and Cellular Probes. 24:77-86.

A microarray approach for simultaneous detection of multiple pathogens in food - (Abstract Only) - (01-Oct-09)

Rapid identification of Listeria spp.: an AOAC performance test of the MIT 1000 rapid microbial identification system - (Abstract Only) - (22-Aug-09)

Development of an oligonucleotide-based microarray to detect multiple foodborne pathogens - (Abstract Only) - (01-Mar-09)

Production of Shiga-like toxins by Escherichia coli O157:H7: Effects of other bacteria and analogues of quorum sensing molecules - (Peer Reviewed Journal)

Tu, S., Uknalis, J., Paoli, G., He, Y., Gehring, A.G. 2009. Production of Shiga-like toxins by Escherichia coli O157:H7: Effects of other bacteria and analogues of quorum sensing molecules. Journal of Rapid Methods and Automation in Microbiology.17(4):420-437.

Capture of E. coli O157:H7 using immunomagnetic beads of different size and antibody conjugating chemistry - (Peer Reviewed Journal)

Tu, S., Reed, S.A., Gehring, A.G., He, Y., Paoli, G. 2009. Capture of E. coli O157:H7 using immunomagnetic beads of different size and antibody conjugating chemistry. Sensors. 9:717-730.

Rapid Confirmation of Listeria spp. with the MIT 1000 Microbial Identification System - (Abstract Only) - (05-Oct-08)

Development of a Multiplex PCR Method for Detection of the Genes Encoding 16S rRNA, Coagulase, Methicillin Resistance and Enterotoxins in Staphylococcus aureus - (Peer Reviewed Journal)

Fan, Y., Pan, F., Paoli, G., Xiao, Y., Sheng, H., Shi, X. 2008. Development of a Multiplex PCR Method for Detection of the Genes Encoding 16S rRNA, Coagulase, Methicillin Resistance and Enterotoxins in Staphylococcus aureus. Journal of Rapid Methods and Automation in Microbiology.16(4):394-411.

Applications of Immunomagnetic Capture and Time-Resolved Fluorescence Detection for Salmonella Enteritidis in Liquid Eggs - (Proceedings)

Tu, S., Gehring, A.G., Paoli, G. 2008. Applications of Immunomagnetic Capture and Time-Resolved Fluorescence Detection for Salmonella Enteritidis in Liquid Eggs. International Society of Optical Engineering (SPIE).6983:1-5 . Optical Engineering.

Innovative applications of bacteriophages in rapid detection and identification of foodborne pathogens - (Abstract Only)

Paoli, G. 2008. Innovative applications of bacteriophages in rapid detection and identification of foodborne pathogens. Meeting Abstract.

DETECTION OF SALMONELLA ENTERITIDIS IN LIQUID EGGS BY IMMUNOMAGNETIC CAPTURE AND TIME-RESOLVED FLUORESCENCE - (Abstract Only)

Tu, S., Gehring, A.G., Paoli, G. 2008. Detection of salmonella enteritidis in liquid eggs by immunomagnetic capture and time-resolved fluorescence. Meeting Abstract.Available: http://spie.org/x648.xml?product_id=786848&show/Abstracts=true&origin_id=x648&event_id=795339&start_at=41

Binding of Nontarget Organisms to Anti-Salmonella and Anti-E. coli O157 Immunomagnetic Beads: Most Probable Composition of Background Eubacteria - (Peer Reviewed Journal)

Irwin, P.L., Nguyen, L., Chen, C., Paoli, G. 2008. Binding of Nontarget Organisms to Anti-Salmonella and Anti-E. coli O157 Immunomagnetic Beads: Most Probable Composition of Background Eubacteria. Analytical and Bioanalytical Chemistry. 391:525-536.

A coupled anti-L. monocytgenes scFv immunomagnetic bead and PCR method for capture and detection of L. monocytogenes from food - (Abstract Only)

Paoli, G., Tu, S., Fan, Y., Shi, X. 2008. A coupled anti-L. monocytgenes scFv immunomagnetic bead and PCR method for capture and detection of L. monocytogenes from food . Meeting Abstract.

Multiple Pathogen Detection Using Biosensors: Advancements and Challenges - (Abstract Only)

Tu, S., Gehring, A.G., Paoli, G. 2007. Multiple Pathogen Detection Using Biosensors: Advancements and Challenges. [Abstract]p.59

SPR Biosensor for the Detection of L. monocytogenes using Phage Displayed Antibody - (Peer Reviewed Journal) - (05-Apr-07)

A LISTERIA MONOCYTOGENES-SPECIFIC SINGLE CHAIN ANTIBODY BIDNS TO THE ACTIN POLYMERIZATION PROTEIN - (Peer Reviewed Journal)

Paoli, G., Brewster, J.D. 2007. A listeria monocytogenes-specific single chain antibody bidns to the actin polymerization protein. Applied and Environmental Microbiology. 15(2007)77-91.

Detection of salmonella in liquid eggs by immunomagnetic capture and time-resolved fluorescence - (Peer Reviewed Journal)

Tu, S., Gehring, A.G., Paoli, G. 2007. Detection of salmonella in liquid eggs by immunomagnetic capture and time-resolved fluorescence. Journal of Rapid Methods and Automation in Microbiology. 15:107-119

DEVELOPMENT OF LISTERIA MONOCYTOGENES-SPECIFIC IMMUNOMAGNETIC BEADS USING A SINGLE-CHAIN ANTIBODY FRAGMENT - (Peer Reviewed Journal)

Paoli, G., Kleina, L.G., Brewster, J.D. 2007. Development of listeria monocytogenes-specific immunomagnetic beads using a single-chain antibody fragment. Foodborne Pathogens and Disease. 4:74-83

IMMUNOMAGNETIC-ELECTROCHEMILUMINESCENT DETECTION OF ESCHERICHIA COLI 0157 IN GROUND BEEF AFTER PRE-ENRICHMENT IN BRILLIANT GREEN BILE BROTH, MODIFIED ESCHERICHIA COLI BROTH WITH OR WITHOUT NOVOBIOCIN, OR GRAM NEGATIVE BROTH - (Peer Reviewed Journal)

Feder, I.E., Wijey, C., Paoli, G., Crawford, C.G., Tu, S. 2007. Immunomagnetic-electrochemiluminescent detection of escherichia coli 0157 in ground beef after pre-enrichment in brilliant green bile broth, modified escherichia coli broth with or without novobiocin, or gram negative broth. Journal of Rapid Methods and Automation in Microbiology. 15:92-106.

SENSITIVE DETECTION OF ESCHERICHIA COLI 0157:H7 BY THE USE OF IMMUNOMAGNETIC AND FLUORESCENT BEADS - (Abstract Only)

Tu, S., Cooke, P.H., Paoli, G., Gehring, A.G. 2006. Sensitive detection of escherichia coli 0157:h7 by the use of immunomagnetic and fluorescent beads. Meeting Abstract.

OXYGEN CONSUMPTION RATE OF CAMPYLOBACTER JEJUNI DURING GROWTH AND SURVIVAL UNDER VARIOUS OXYGEN LEVELS - (Abstract Only)

Chen, C., Paoli, G., Irwin, P.L. 2006. Oxygen consumption rate of campylobacter jejuni during growth and survival under various oxygen levels. {Abstract}. International Association of Food Protection. P4-59.

IDENTIFICATION OF THE SURFACE ANTIGEN RECOGNIZED BY A LISTERIA MONOCYTOGENES-SPECIFIC PHAGE-DISPLAYED ANTIBODY FRAGMENT AND ITS PRESENCE IN DIFFERENT PHYSIOLOGICAL CONDITIONS - (Peer Reviewed Journal)

Paoli, G., Brewster, J.D. 2007. Identification of the surface antigen recognized by a listeria monocytogenes-specific phage-displayed antibody fragment and its presence in different physiological conditions. Journal of Rapid Methods and Automation in Microbiology. Vol. 4(1):74-83.

DETECTION OF ESCHERICHIA COLI 0157:H7 THROUGH THE FORMATION OF SANDWICHED COMPLEXES WITH IMMUNOMAGNETIC AND FLUORESCENT BEADS - (Peer Reviewed Journal)

Tu, S., Golden, M., Cooke, P.H., Luce, L., Paoli, G., Gehring, A.G.2005 Detection of escherichia coli 0157:h7 through the formation of sandwiched complexes with immunomagnetic and fluorescent beads. Journal of Rapid Methods and Automation in Microbiology. 13:269-282

LISTERIA MONOCYTOGENES - (Book / Chapter)

Paoli, G., Bhunia, A., Bayles, D. 2005. Listeria monocytogenes. Foodborne Pathogens: Microbiology and Molecular Biology Reviews. Horizon Scientific Press, Norwich, UK. Chapter 15. p. 295-325.

BIOSENSOR-BASED PROCESSES FOR DETECTION OF BACTERIA IN THE FOOD PROCESSING ENVIRONMENT - (Abstract Only)

Tu, S., Gehring, A.G., Paoli, G. 2005. Biosensor-based processes for detection of bacteria in the food processing environment. International Food Technology Meeting Abstracts. Paper No. 6-4.

DEVELOPMENT OF BIOSENSORS FROM PHAGE DISPLAYED ANTIBODIES - (Proceedings)

Paoli, G., Brewster, J.D., Kleina, L.G. 2004. Development of biosensors from phage displayed antibodies. UJNR Food & Agricultural Panel Proceedings. pp. 359-364.

TIME-RESOLVED FLUORESCENCE DETECTION OF TOXINS AND PATHOGENIC BACTERIA IN FOODS - (Peer Reviewed Journal)

Tu, S., Golden, M., Paoli, G., Gore, M., Gehring, A.G. 2004. Time-resolved fluorescence detection of toxins and pathogenic bacteria in foods. Journal of Rapid Methods and Automation in Microbiology. 12. pp. 247-258.

DETECTION OF VIABLE BACTERIA CELLS BY BIOLUMINESCENCE: A BIOENERGETIC APPROACH - (Peer Reviewed Journal)

Tu, S., Wijey, C., Paoli, G., Gehring, A.G., Irwin, P.L. 2004. Detection of viable bacteria cells by bioluminescence: a bioenergetic approach. Journal of Rapid Methods and Automation in Microbiology. V.12, #3, p. 207-219.

DEVELOPMENT OF BIOSENSORS FOR DETECTION OF FOOD-BORNE PATHOGENS BY ANTIBODY PHAGE - (Abstract Only)

Paoli, G., Brewster, J.D. 2004. Development of biosensors for detection of food-borne pathogens by antibody phage. (Meeting Abstract). 68(1):146-149.

IMMUNOLOGICAL BIOSENSOR-BASED METHODS - (Proceedings)

Paoli, G., Brewster, J.D. 2004. Immunological biosensor-based methods. American Chemical Society Abstracts.

TIME-RESOLVED FLUORESCENCE DETECTION OF SHIGA-LIKE TOXINS PRODUCED BY ESCHERICHIA COLI 0157 AND NON-0157 IN GROUND BEEF - (Abstract Only)

Tu, S., Golden, M., Paoli, G., Gehring, A.G., Gore, M. 2004. Time-resolved fluorescence detection of shiga-like toxins produced by escherichia coli 0157 and non-0157 in ground beef. Meeting Abstract.

SINGLE CHAIN ANTIBODY WITH SPECIFICITY FOR LISTERIA MONOCYTOGENES - (Peer Reviewed Journal)

Paoli, G., Chen, C., Brewster, J.D. 2004. Single chain antibody with specificity for listeria monocytogenes. Journal of Immunological Methods.Vol 289. page 147-155.

CHARACTERIZATION OF A LISTERIA MONOCYTOGENES SPECIES-SPECIFIC SINGLE-CHAIN PHAGE ANTIBODY - (Abstract Only)

Paoli, G., Brewster, J.D. 2004. Characterization of a listeria monocytogenes species-specific single-chain phage antibody. American Society for Microbiology Annual Meeting.

EFFECT OF BACTERIAL PROTEIN EXTRACTION REAGENT AND SODIUM AZIDE ON THE DETECTION OF ESCHERICHIA COLI 0157:H7 USING IMMUNOMAGNETIC-ELECTROCHEMILUMINESCENCE - (Peer Reviewed Journal)

Wijey, C., Crawford, C.G., Tu, S., Paoli, G. Effect of bacterial protein extraction reagent and sodium azide on the detection of escherichia coli 0157:h7 using immunomagnetic-electrochemiluminescence. Journal of Rapid Methods and Automation in Microbiology. 2004. 12. pp. 115-126.

SELECTIVE ISOLATION OF LISTERIA MONOCYTOGENES FROM FOODS USING A SINGLE-CHAIN ANTIBODY FRAGMENT DERIVED BY PHAGE DISPLAY - (Abstract Only)

Brewster, J.D., Paoli, G. 2004. Selective isolation of listeria monocytogenes from foods using a single-chain antibody fragment derived by phage display. Meeting Abstract. Granada, Spain. Paper #4.

BIOSENSORS FOR THE DETECTION OF FOOD-BORNE PATHOGENS - (Abstract Only)

Paoli, G., Tu, S. 2003. Biosensors for the detection of food-borne pathogens. Eastern Food Science Conference.