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United States Department of Agriculture

Agricultural Research Service

Related Topics

David H Kingsley
Food Safety and Intervention Technologies
Molecular Biologist

Phone: (302) 857-6406
Fax: (302) 857-6451
Room 32
JAMES W.W. BAKER CENTER
DSU, 1200 N DUPONT HIGHWAY
DOVER, DE, 19901-2277

Projects
Assessment of Inactivation and Persistence of Human Norovirus
General Cooperative Agreement (A)
  Accession Number: 427048
Pathogen Detection and Intervention Methods for Shellfish
Appropriated (D)
  Accession Number: 420935
Process Efficacy Investigation of Infectious Norovirus Particle Ratios and Enteric Viral Surrogates Levels in Environmental and Food Samples
Interagency Reimbursable Agreement (I)
  Accession Number: 426742

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Pathogen reduction in human plasma using an ultrashort pulsed laser - (Peer Reviewed Journal) - (30-Sep-14)
High pressure processing of bivalve shellfish and HPP's potential use as a virus intervention - (Review Article)
Kingsley, D.H. 2014. High pressure processing of bivalve shellfish and HPP's potential use as a virus intervention. Review Article. DOI:10.3390/foods3020336.
Desirability of oysters treated by high pressure processing at different temperatures and elevated pressures - (Peer Reviewed Journal)
Kingsley, D.H., Kuhn, D.D., Flick, G.J., Oh, J., Lawson, L.S., Meade, G.K., Giesecke, C.C. 2014. Desirability of oysters treated by high pressure processing at different temperatures and elevated pressures. American Journal of Food Technology. 9:209-216.
Inactivation of human norovirus in contaminated oysters and clams by high-hydrostatic pressure - (Peer Reviewed Journal)
Ye, M., Li, X., Kingsley, D.H., Jiang, X., Chen, H. 2014. Inactivation of human norovirus in contaminated oysters and clams by high-hydrostatic pressure. Applied and Environmental Microbiology. 80:2248-2253.
Studies of Inactivation Mechanism of non-enveloped icosahedral viruses by a visible ultrashort pulsed laser - (Peer Reviewed Journal)
Tsen, S.D., Kingsley, D.H., Poweleit, C., Achilefu, S., Soroka, D.S., Wu, T., Tsen, K. 2014. Studies of Inactivation Mechanism of non-enveloped icosahedral viruses by a visible ultrashort pulsed laser. Virology Journal. 11:20.
Inactivation of human norovirus using chemical sanitizers - (Peer Reviewed Journal)
Kingsley, D.H., Vincent, E., Meade, G.K., Watson, C., Fan, X. 2014. Inactivation of human norovirus using chemical sanitizers. International Journal of Food Microbiology. 171:94-99.
Temperature effects for high pressure processing of Picornaviruses - (Peer Reviewed Journal)
Kingsley, D.H., Li, X., Chen, H. 2014. Temperature effects for high pressure processing of Picornaviruses. Food and Environmental Virology. 6:58-61.
The influence of temperature pH and water immersion on the high hydrostatic pressure inactivation of GI.1 and GII.4 human noroviruses - (Peer Reviewed Journal)
Li, X., Chen, H., Kingsley, D.H. 2013. The influence of temperature pH and water immersion on the high hydrostatic pressure inactivation of GI.1 and GII.4 human noroviruses. International Journal of Food Microbiology. 167:138-143.
Sensitivity of hepatitis A and murine norovirus to electron beam irradiation in oyster homogenates and whole oysters - quantifying the reduction in potential infection risks - (Peer Reviewed Journal)
Nair, C., Dancho, B.A., Kingsley, D.H., Calci, K., Meade, G.K., Mena, K.D., Pillai, S. 2013. Sensitivity of hepatitis A and murine norovirus to electron beam irradiation in oyster homogenates and whole oysters - quantifying the reduction in potential infection risks. Applied and Environmental Microbiology. 79:3796-3801.
Shellfish contamination and spoilage - (Book / Chapter)
Kingsley, D.H. 2014. Shellfish contamination and spoilage.In: Batt, C.A., Totorello, M.L., Elsevier Ltd., Encyclopedia of Food Microbiology. Volume 3, pp.389-396.
Correlation between lack of norovirus replication and histo-blood group antigen expression in 3D-intestinal epithelial cultures - (Peer Reviewed Journal)
Herbst-Kralovets, M.M., Radtke, A.L., Lay, M.K., Bolick, A.N., Sarker, S.S., Kingsley, D.H., Arntzen, C.J., Estes, M.K., Nickerson, C. 2013. Correlation between lack of norovirus replication and histo-blood group antigen expression in 3D-intestinal epithelial cultures. Emerging Infectious Diseases. Volume 19(3):431-438.
High pressure processing and its application to the challenge of virus-contaminated foods - (Review Article)
Kingsley, D.H. 2013. High pressure processing and its application to the challenge of virus-contaminated foods. Food and Environmental Virology. 5:1-12.
Resilience of norovirus GII.4 to freezing and thawing:implications for virus infectivity - (Peer Reviewed Journal)
Richards, G.P., Watson, M.A., Meade, G.K., Hovan, G.L., Kingsley, D.H. 2012. Resilience of norovirus GII.4 to freezing and thawing:implications for virus infectivity. Food and Environmental Virology. 4:192-197.
Novel methods for detection of foodborne viruses - (Abstract Only)
Kingsley, D.H. 2012. Novel methods for detection of foodborne viruses. Meeting Abstract., American Chemical Society (ACS) meeting., Philadelphia, PA., August 19-23, 2012., Volume 1, Page 1.
Recent intervention and detection advances for shellfish-borne norovirus - (Abstract Only)
Kingsley, D.H. 2012. Recent intervention and detection advances for shellfish-borne norovirus. Meeting Abstract. National Committee for the Microbiologist Criteria Subcommittee., Washington, DC., May 8-10,2012.Volume 1, Page 1.
Food safety research at Delaware State University: keeping the runs from slowing you down - (Abstract Only)
Kingsley, D.H. 2012. Food safety research at Delaware State University: keeping the runs from slowing you down. Meeting Abstract.Delaware State University, College of Agriculture and Related Sciences, Dover, DE, April 13, 2012., Volume 1, Page 1.
An extraction method for discrimination between infectious and inactive norovirus using RT-PCR - (Abstract Only)
Kingsley, D.H., Dancho, B. 2012. An extraction method for discrimination between infectious and inactive norovirus using RT-PCR. Meeting Abstract. American Society for Virology., Madison, Wisconsin., July 21-25, 2012., Volume 1, Page 1.
Rapid extraction of virus-contaminated hemocytes from oysters - (Peer Reviewed Journal) - (17-Sep-11)
Hemocytes are sites of persistence for virus-contaminated oysters - (Peer Reviewed Journal)
Provost, K., Ozbay, G., Anderson, R., Richards, G.P., Kingsley, D.H. 2011. Hemocytes are sites of persistence for virus-contaminated oysters. Applied and Environmental Microbiology. 77:8360-8369.
Oyster hemocyte mobilization and increased adhesion activity after beta glucan administration - (Peer Reviewed Journal)
Anderson, R., Gulnihal, O., Kingsley, D.H., Maureen, S.A. 2011. Oyster hemocyte mobilization and increased adhesion activity after beta glucan administration. Journal of Shellfish Research. 30(3):635-641.
New perspectives on virus detection in shellfish: hemocytes as a source of concentrated virus - (Abstract Only) - (13-Jun-11)
Human norovirus inactivation in oysters by high hydrostatic pressure processing: A randomized double-blinded study - (Peer Reviewed Journal)
Leon, J., Kingsley, D.H., Montes, J., Richards, G.P., Lyon, G., Abdulhafid, G., Seitz, S., Fernandez, M., Teunis, P.F., Flick, G.J., Moe, C.L. 2011. Human norovirus inactivation in oysters by high hydrostatic pressure processing: A randomized double-blinded study. Journal of Infectious Diseases. 77(15):5476-82.
USDA food-borne virus research initiatives at Delaware State University - (Abstract Only) - (09-Apr-11)
Mechanisms of viral persistence within bivalves - (Abstract Only) - (17-Dec-10)
High pressure processing as an intervention for raw virus-contaminated shellfish - (Abstract Only) - (17-Dec-10)
New targets for expedient detection of viruses within shellfish - (Abstract Only)
Kingsley, D.H. 2011. New targets for expedient detection of viruses within shellfish [abstract]. 1st International Symposium on Fisheries, Agriculture and Food Hygiene. February 20-28, 2011, Lima,Peru. p.1.
Development of rapid hemocyte-based extraction methods for detection of hepatitis A virus and murine norovirus in contaminated oysters - (Abstract Only)
Dancho,B.,Kingsley,D.2010.Development of rapid hemocyte-based extraction methods for detection of hepatitis A virus and murine norovirus in contaminated oysters [abstract].Interstate Seafood Seminar.Ocean City,MD.p.1.
Persistence of enteric viruses within oysters (Crassostrea virginica) - (Abstract Only)
Kingsley, D. 2010. Persistance of enteric viruses within oysters (Crassostrea virginica) [abstract].Interstate Seafood Seminar.Ocean City,MD.p.1.
High hydrostatic pressure processing of murine norovirus 1-contaminated oysters inhibits oral infection in STAT-1 -/- deficient female mice - (Peer Reviewed Journal)
Gogal, R.M., Kerr, R., Kingsley, D.H., Granata, L.A., Leroith, T., Holliman, S.D., Dancho, B.A., Flick, G.J. 2011. High hydrostatic pressure processing of murine norovirus 1-contaminated oysters inhibits oral infection in STAT-1 -/- deficient female mice. Journal of Food Protection. 74:209-214.
Potential virus detection and intervention methods for molluscan shellfish - (Abstract Only)
Kingsley, D.H. 2010. Potential virus detection and intervention methods for molluscan shellfish. [abstract]. Indiana Molecular Biology Institute, Indiana University. p. 1.
High pressure processing inactivates human norovirus within oysters - (Abstract Only)
Kingsley, D.H., Leon, J.S., Richards, G.P., Flick, G., Lyon, G.M., Abdulhafid, G., Sobolik, J., Creadore, A., Seitz, S., Smith, E., Fernandez, M., Moe, C. 2010. High pressure processing inactivates human norovirus within oysters [abstract]. Annual Meeting at American Society for Virology. p. 1.
A Potential Mechanism of Virus Persistence within Bivalve Shellfish. - (Abstract Only)
Kingsley,D.,Provost,K. 2010.A Potential Mechanism of Virus Persistance within Bivalve Shellfish [abstract].American Society of Virology.Bozeman,Montana.p.1.
Retention of Enteric Viruses by the Hemocytes of the Eastern Oyster (Crassostrea virginica) - (Abstract Only)
Kingsley,D.,Provost,K.,Anderson,R.,Ozbay,G.2010.Retention of enteric viruses by the hemocytes of the Eastern Oyster (Crassostrea virginica)[abstract].IFT Annual Meeting.Chicago,IL.p.1.
Analysis of norovirus contamination of seafood - (Abstract Only)
Arntzen, C., Kingsley, D.H., Herbst-Kralovetz, M., Lay, M., Sarker, S., Atmar, R., Estes, M., Nickerson, C. 2010. Analysis of norovirus contamination of seafood. [abstract]. Institute of Food Technologists. Annual Meeting and NIFA NRI/AFRI Meeting. p. 1.
Retention of Enteric Viruses by the Hemocytes of the Eastern Oyster (Crassostrea virginica) - (Abstract Only)
Ozbay,G.,Kingsley,D.,Provost,K.,Anderson,R.2010.Retention of Enteric Viruses by the Hemocytes of the Eastern Oyster (Crassostrea virginica)[abstract].Institute of Food Technologists Meeting. Chicago,IL.p.1.
Rapid detection of hepatitis A virus and murine norovirus in hemocytes of contaminated oysters - (Abstract Only)
Dancho,B.,Kingsley,D. 2010. Rapid detection of hepatitis A virus and murine norovirus in hemocytes of contaminated oysters [abstract].American Society of Virology.Bozeman, Montana. p.1.
Novel Platform Technologies for Analysis of Norovirus Contamination of Sea Food - (Abstract Only)
Arntzenl,C.,Kingsley,D.,Herbst-Kralovetz,M.,Sarker,S.,Dienhalt,C.,Nickerson,C. 2010. Novel platform technologies for anaylsis of Norovirus contamination of Seafood [abstract].IFT Meeting.Chicago,IL. p.1.
High Pressure Inactivation of HAV within Mussels - (Peer Reviewed Journal)
Terio, V., Tantillo, G., Martella, V., Dipinto, P., Buonavoglia, C., Kingsley, D.H. 2010. High Pressure Inactivation of HAV within Mussels. Food and Environmental Virology. 2:83-88.
Challenges for Norovirus Detection within Shellfish - (Abstract Only)
Kingsley,D.,2009.Challenges for Novovirus detection within shellfish [abstract].Purdue/ARS Meeting.West Lafayette, Indiana.p.1.
Norovirus: Human Health and Food-borne Implications - (Abstract Only)
Kingsley,D.2009.Norovirus: Human Health and Foodborne implications [abstract].Anne Arundel County Health Laboratory.Annapolis,MD. p.1.
High pressure inactivation of HAV within oysters: comparison of shucked oysters with whole in shell meats - (Peer Reviewed Journal)
Kingsley, D.H., Calci, K., Holliman, S., Dancho, B.A., Flick, G. 2009. High pressure inactivation of HAV within oysters: comparison of shucked oysters with whole in shell meats. Food and Environmental Virology. 1:137-140.
Food-Borne Noroviruses - (Book / Chapter) - (17-Jun-09)
Detection, isolation, and persistence of viruses within bivalve mollusks - (Abstract Only)
Kingsley,D. 2009.Detection,isolation and persistance of viruses within bivalve mollusks [abstract]. University of Bari,Italy. p. 1.
Inactivation of HAV and norovirus surrogates within raw shellfish and other foods - (Abstract Only)
Kingsley,D. 2009. Inactivateion of HAV and norovirus surrogates within raw shellfish and other foods [abstract]. University of Bari,Italy. p.1.
Influence of pH, Salt and Temperature on Pressure Inactivation of Hepatitis A virus - (Peer Reviewed Journal)
Kingsley, D.H., Chen, H. 2009. Influence of pH, Salt and Temperature on Pressure Inactivation of Hepatitis A virus. Food Microbiology. 130:61-64.
Evaluation of High Pressure Effects on Food-borne Viruses - (Proceedings) - (10-Nov-08)
High Pressure Inactivation of Food-borne Viruses - (Abstract Only)
Kingsley,D. 2008.High Pressure Inactivation of Foodborne viruses [abstract].Conference on High Pressure Bioscience and Biotechnology.LaJolla,CA.p.1.
Pressure Inactivation of Vibrio parahaemolyticus in Oysters- Influence of Pressure level and Treatment Temperature - (Peer Reviewed Journal)
Kural, A.G., Shearer, A., Kingsley, D.H., Chen, H. 2008. Pressure Inactivation of Vibrio parahaemolyticus in Oysters- Influence of Pressure level and Treatment Temperature. International Journal of Food Microbiology. 127:1-5.
PERSISTANCE OF VIRUSES IN OYSTER (CRASSOSTREA VIRGINICA) HEMOCYTES - (Abstract Only)
Ozbay,G.,Kingsley,D.2008 Persistance of Viruses in Oyster (Crassostrea Virginica)Hemocytes.Join 1890 AEA/ARD Land Grant Conference.Memphis,TN. p.1.
Aqueous Matrix Compositions Influence Virus Inactivation by High Pressure Processing - (Peer Reviewed Journal)
Kingsley, D.H., Chen, H. 2008. Aqueous Matrix Compositions Influence Virus Inactivation by High Pressure Processing. Journal of Food Protection. 71(1):598-603.
AN RNA EXTRACTION PROTOCOL FOR SHELLFISH-BORNE VIRUSES - (Peer Reviewed Journal)
Kingsley, D.H. 2007. An rna extraction protocol for shellfish-borne viruses. Journal of Virological Methods. Vol.141:58-62.
Inactivation of a Norovirus by High Pressure Processing - (Peer Reviewed Journal)
Kingsley, D.H., Holliman, D., Calci, K., Chen, H., Flich, G. 2007. Inactivation of a Norovirus by High Pressure Processing. Applied and Environmental Microbiology. 73:581-585.
INACTIVATION OF HEPATITIS A VIRUS BY HIGH PRESSURE PROCESSING: THE ROLE OF TEMPERATURE AND PRESSURE OSCILLATION - (Peer Reviewed Journal)
Kingsley, D.H., Guan, D., Hoover, D.G., Chen, H. 2006. Inactivation of hepatitis a virus by high pressure processing: the role of temperature and pressure oscillation. Journal of Food Protection. 69:2454-2459
TEMPERATURE AND TREATMENT TIME INFLUENCES PRESSURE INACTIVATION OF FELINE CALICIVIRUS, A NOROVIRUS SURROGATE - (Peer Reviewed Journal)
Chen, H., Hoover, D., Kingsley, D.H. 2005. Temperature and treatment time influences pressure inactivation of feline calicivirus, a norovirus surrogate. Journal of Food Protection. 68: 2389-2394.
PRESSURE INACTIVATION OF HEPATITIS A VIRUS IN STRAWBERRY PUREE AND SLICED GREEN ONIONS - (Peer Reviewed Journal)
Kingsley, D.H., Guan, D., Hoover, D.G. 2005. Pressure inactivation of hepatitis a virus in strawberry puree and sliced green onions. Journal of Food Protection. 68:1748-1751.
HIGH PRESSURE INACTIVATION OF HEPATITIS A VIRUS WITHIH OYSTERS - (Peer Reviewed Journal)
Calci, K.R., Tezloff, R.C., Kingsley, D.H., Meade, G.K. 2005. High pressure inactivation of hepatitis a virus withih oysters. Applied and Environmental Microbiology. Vol. 71. No.1
HIGH HYDROSTATIC PRESSURE AS AN INTERVENTION STRATEGY FOR VIRUS-CONTAMINATED SHELLFISH - (Abstract Only)
Kingsley, D.H., Calci, K., Hoover, D. 2004. High hydrostatic pressure as an intervention strategy for virus-contaminated shellfish. American Society for Virology. P33-1. pg. 266.
INACTIVATION OF SELECTED PICORNAVIRUS BY HIGH HYDROSTATIC PRESSURE - (Peer Reviewed Journal)
Kingsley, D.H., Chen, H., Hoover, D.G. 2004. Inactivation of selected picornavirus by high hydrostatic pressure. Virus Research. Vol. 102. pg. 221-224. 2004.
A SYBR GREEN REAL-TIME RT-PCR METHOD TO DETECT AND QUANTITATE NORWALK VIRUS IN STOOLS - (Peer Reviewed Journal)
RICHARDS, G.P., WATSON, M.A., KINGSLEY, D.H. A SYBR GREEN REAL-TIME RT-PCR METHOD TO DETECT AND QUANTITATE NORWALK VIRUS IN STOOLS. JOURNAL OF VIROLOGICAL METHODS. 2004. vol. 116. pg. 67-70.
HIGH HYDROSTATIC PRESSURE PROCESSING: A POTENTIAL SOLUTION FOR SHELLFISH-BORNE VIRUSES - (Abstract Only)
KINGSLEY, D.H. HIGH HYDROSTATIC PRESSURE PROCESSING: A POTENTIAL SOLUTION FOR SHELLFISH-BORNE VIRUSES. MEETING ABSTRACT. 2003. p. 65-74.
PRESSURE INACTIVATION KINETICS OF BACTERIOPHAGE LAMBDA CI 857 - (Peer Reviewed Journal)
Chen, H., Joerger, R.D., Hoover, D.G., Kingsley, D.H. 2004. Pressure inactivation kinetics of bacteriophage lambda ci 857. Journal of Food Protection. 67:505-511.
MITIGATION OF HAV IN SHUCKED OYSTERS USING HIGH HYDROSTATIC PRESSURE TREATMENT - (Abstract Only)
CALCI, K.R., KINGSLEY, D.H., REDDY, R.N. MITIGATION OF HAV IN SHUCKED OYSTERS USING HIGH HYDROSTATIC PRESSURE TREATMENT. MEETING ABSTRACT. 2003. P275.
VIRUS AND VIBRIO INITIATIVES WITHIN THE USDA MICROBIAL FOOD SAFETY RESEARCH UNIT - (Other)
KINGSLEY, D.H. VIRUS AND VIBRIO INITIATIVES WITHIN THE USDA MICROBIAL FOOD SAFETY RESEARCH UNIT. UNITED STATES JAPAN NATURAL RESOURCES PROTEIN PANEL. N1-N13
MOLECULAR DETECTION AND HIGH PRESSURE SANITIZATION OF SHELLFISH-BORNE VIRUSES - (Proceedings)
KINGSLEY, D.H. MOLECULAR DETECTION AND HIGH PRESSURE SANITIZATION OF SHELLFISH-BORNE VIRUSES. MEETING ABSTRACT. 2003. p. 475-483.
PERSISTENCE OF HEPATITIS A VIRUS WITHIN OYSTERS - (Peer Reviewed Journal)
KINGSLEY, D.H., RICHARDS, G.P. PERSISTENCE OF HEPATITIS A VIRUS WITHIN OYSTERS. JOURNAL OF FOOD PROTECTION. 2003. Vol. 66 (2) p. 331-334
DETECTION OF BOTH HEPATITIS A VIRUS AND NORWALK-LIKE VIRUS IN IMPORTED CLAMS ASSOCIATED WITH FOOD-BORNE ILLNESS - (Peer Reviewed Journal) - (01-May-02)
INACTIVATION OF HEPATITIS A AND A CALICIVIRUS BY HIGH HYDROSTATIC PRESSURE - (Peer Reviewed Journal) - (25-Apr-02)
ARS News Articles
Last Modified: 10/31/2014
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