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David H Kingsley
Microbial Food Safety Research Unit
Molecular Biologist

Phone: (302) 857-6406
Fax: (302) 857-6451
Room 32
JAMES W.W. BAKER CENTER
DSU, 1200 N DUPONT HIGHWAY
DOVER, DE, 19901-2277

Projects
Molecular & Biochemical Detection & Intervention Methods for Bacterial and Viral Pathogens in Aquaculture Products
Appropriated (D)
  Accession Number: 410793
Development of Methods and Strategies to Improve the Microbiological Safety of Aquaculture Products
Nonfunded Cooperative Agreement (N)
  Accession Number: 411443
Norwalk Virus Inactivation by High Hydrostatic Pressure Processing: a Comprehensive and Integrated Program for Research and Outreach
Nonfunded Cooperative Agreement (N)
  Accession Number: 410249
Evaluation of Virus Inactivation by Hydrostatic Pressure
Nonfunded Cooperative Agreement (N)
  Accession Number: 408306
Validation of the Effect of Interventions and Process on Persistence of Pathogens on Food
Nonfunded Cooperative Agreement (N)
  Accession Number: 414633
Development of a Robust Assay for Infective Noroviruses, for Use in Food Safety Diagnostics
Reimbursable (R)
  Accession Number: 414903

Publications
High pressure inactivation of HAV within oysters: comparison of shucked oysters with whole in shell meats - (Peer Reviewed Journal) - (19-Aug-09)
Influence of pH, Salt and Temperature on Pressure Inactivation of Hepatitis A virus - (Peer Reviewed Journal)
Kingsley, D.H., Chen, H. 2009. Influence of pH, Salt and Temperature on Pressure Inactivation of Hepatitis A virus. Food Microbiology. 130:61-64.
Evaluation of High Pressure Effects on Food-borne Viruses - (Proceedings/Symposium) - (10-Nov-08)
Pressure Inactivation of Vibrio parahaemolyticus in Oysters- Influence of Pressure level and Treatment Temperature - (Peer Reviewed Journal)
Kural, A.G., Shearer, A., Kingsley, D.H., Chen, H. 2008. Pressure Inactivation of Vibrio parahaemolyticus in Oysters- Influence of Pressure level and Treatment Temperature. International Journal of Food Microbiology. 127:1-5.
Aqueous Matrix Compositions Influence Virus Inactivation by High Pressure Processing - (Peer Reviewed Journal)
Kingsley, D.H., Chen, H. 2008. Aqueous Matrix Compositions Influence Virus Inactivation by High Pressure Processing. Journal of Food Protection. 71(1):598-603.
An Rna Extraction Protocol for Shellfish-Borne Viruses - (Peer Reviewed Journal)
Kingsley, D.H. 2007. An rna extraction protocol for shellfish-borne viruses. Journal of Virological Methods. Vol.141:58-62.
Inactivation of a Norovirus by High Pressure Processing - (Peer Reviewed Journal)
Kingsley, D.H., Holliman, D., Calci, K., Chen, H., Flich, G. 2007. Inactivation of a Norovirus by High Pressure Processing. Applied and Environmental Microbiology. 73:581-585.
Inactivation of Hepatitis a Virus by High Pressure Processing: the Role of Temperature and Pressure Oscillation - (Peer Reviewed Journal)
Kingsley, D.H., Guan, D., Hoover, D.G., Chen, H. 2006. Inactivation of hepatitis a virus by high pressure processing: the role of temperature and pressure oscillation. Journal of Food Protection. 69:2454-2459
Temperature and Treatment Time Influences Pressure Inactivation of Feline Calicivirus, a Norovirus Surrogate - (Peer Reviewed Journal)
Chen, H., Hoover, D., Kingsley, D.H. 2005. Temperature and treatment time influences pressure inactivation of feline calicivirus, a norovirus surrogate. Journal of Food Protection. 68: 2389-2394.
Pressure Inactivation of Hepatitis a Virus in Strawberry Puree and Sliced Green Onions - (Peer Reviewed Journal)
Kingsley, D.H., Guan, D., Hoover, D.G. 2005. Pressure inactivation of hepatitis a virus in strawberry puree and sliced green onions. Journal of Food Protection. 68:1748-1751.
High Pressure Inactivation of Hepatitis a Virus Withih Oysters - (Peer Reviewed Journal)
Calci, K.R., Tezloff, R.C., Kingsley, D.H., Meade, G.K. 2005. High pressure inactivation of hepatitis a virus withih oysters. Applied and Environmental Microbiology. Vol. 71. No.1
High Hydrostatic Pressure As An Intervention Strategy for Virus-Contaminated Shellfish - (Abstract)
Kingsley, D.H., Calci, K., Hoover, D. 2004. High hydrostatic pressure as an intervention strategy for virus-contaminated shellfish. American Society for Virology. P33-1. pg. 266.
Inactivation of Selected Picornavirus by High Hydrostatic Pressure - (Peer Reviewed Journal)
Kingsley, D.H., Chen, H., Hoover, D.G. 2004. Inactivation of selected picornavirus by high hydrostatic pressure. Virus Research. Vol. 102. pg. 221-224. 2004.
A Sybr Green Real-Time Rt-Pcr Method to Detect and Quantitate Norwalk Virus in Stools - (Peer Reviewed Journal)
RICHARDS, G.P., WATSON, M.A., KINGSLEY, D.H. A SYBR GREEN REAL-TIME RT-PCR METHOD TO DETECT AND QUANTITATE NORWALK VIRUS IN STOOLS. JOURNAL OF VIROLOGICAL METHODS. 2004. vol. 116. pg. 67-70.
High Hydrostatic Pressure Processing: a Potential Solution for Shellfish-Borne Viruses - (Abstract)
KINGSLEY, D.H. HIGH HYDROSTATIC PRESSURE PROCESSING: A POTENTIAL SOLUTION FOR SHELLFISH-BORNE VIRUSES. MEETING ABSTRACT. 2003. p. 65-74.
Pressure Inactivation Kinetics of Bacteriophage Lambda Ci 857 - (Peer Reviewed Journal)
Chen, H., Joerger, R.D., Hoover, D.G., Kingsley, D.H. 2004. Pressure inactivation kinetics of bacteriophage lambda ci 857. Journal of Food Protection. 67:505-511.
Mitigation of Hav in Shucked Oysters Using High Hydrostatic Pressure Treatment - (Abstract)
CALCI, K.R., KINGSLEY, D.H., REDDY, R.N. MITIGATION OF HAV IN SHUCKED OYSTERS USING HIGH HYDROSTATIC PRESSURE TREATMENT. MEETING ABSTRACT. 2003. P275.
Virus and Vibrio Initiatives Within the USDA Microbial Food Safety Research Unit - (Other)
KINGSLEY, D.H. VIRUS AND VIBRIO INITIATIVES WITHIN THE USDA MICROBIAL FOOD SAFETY RESEARCH UNIT. UNITED STATES JAPAN NATURAL RESOURCES PROTEIN PANEL. N1-N13
Molecular Detection and High Pressure Sanitization of Shellfish-Borne Viruses - (Proceedings/Symposium)
KINGSLEY, D.H. MOLECULAR DETECTION AND HIGH PRESSURE SANITIZATION OF SHELLFISH-BORNE VIRUSES. MEETING ABSTRACT. 2003. p. 475-483.

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Last Modified: 11/21/2009
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